SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene4986

Length: 1885 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene4986 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein [Arabidopsis thaliana] emb\|CAA23048.1\| putative polygalacturonase [Arabidopsis thaliana] emb\|CAB81300.1\| putative polygalacturonase [Arabidopsis thaliana] gb\|AAL32775.1\|	-	-	62%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	51%
Blast2go	glycoside hydrolase family 28 protein	-	-	76%

Source	ECs	Term	Type	e value	Identity
Blast2go	Polygalacturonase.	EC:3.2.1.15	-	0.0	76%

Source	KEGGs	Term	Identity
Blast2go	Pentose and glucuronate interconversions	00040	76%
Blast2go	Starch and sucrose metabolism	00500	76%
Blast2go	Metabolic pathways	01100	76%

Source	GOs	Term	Type	Identity
Blast2go	carbohydrate metabolic process	GO:0005975	Biological Process	76%
Blast2go	cellular cell wall organization	GO:0007047	Biological Process	76%
Blast2go	polygalacturonase activity	GO:0004650	Molecular Function	76%
Blast2go	endomembrane system	GO:0012505	Cellular Component	76%
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	76%

Source	InterPros	Term	Type	Identity
Sma3	Glycoside hydrolase, family 28	IPR000743	-	-
Sma3	Sugar transporter, conserved site	IPR005829	-	-
Sma3	Parallel beta-helix repeat	IPR006626	-	-
Sma3	Pectin lyase fold	IPR012334	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LQW0

Fln msg:

Fln protein: MIIPVAMWSPKMLRVIFILVLIQWGFAVSYGKKGFSRGIRVGIEPKTCIGIVPNKHRENVFSIRYFGAVGDGKTLNTKAFRTAISHIQNLNSRGKGGTLLYIPSGVWLTETFNLTSHLTLYLAKDAVLKASQDTNGWLVIDPLPSYGRGRELPGGRYISFIHGDGLEDVVITGQNGTIDGQGEVWWNMWRDGTLKYTRGGLVELMNSKDVIISNLVLLNSPFWNVHPIYCSNVVVRGLTIIAPRDSPNTDGIDPDSSSNVCIEDCYVSNGDDGIAIKSGWDEYGISFNRPSSNIIVRRITISTPFSGIAIGSETSGGIRDILVENISIYSSTVGIRVKTNVGRGGIIRNITFSHIYLDNVGTGIKFSGNTGDHPDARYNPMALPVVGDIAVLNVVGNSIKTAGSLVGLQRSPFSNICLANIHIQEKVGRSTGSSWDCKDVEGSSIGVTPSPCQQLQSPGSSFCNNAFQLHNFDP*

Protein Length: 476

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTTGTTTTTAGATTTTTCTGCTTCAAATCTG_-_ATGATTATTCCTGTCGCAATGTGGAGTCCAAAGATGCTGCGAGTGATCTTCATTTTGGTTTTGATCCAATGGGGTTTCGCAGTTTCTTATGGCAAGAAGGGGTTTTCCCGTGGCATTAGGGTTGGAATAGAACCGAAAACGTGTATCGGAATAGTTCCGAACAAACATCGGGAAAACGTTTTCTCCATCAGATATTTTGGAGCTGTAGGCGACGGGAAAACTCTCAACACCAAAGCTTTTCGGACGGCAATCTCTCACATTCAAAATCTAAACAGCAGAGGCAAAGGCGGCACATTGCTTTATATTCCGAGCGGAGTTTGGCTTACAGAAACTTTCAATCTTACCAGTCATTTGACTCTGTATTTGGCTAAGGATGCCGTTTTAAAGGCATCACAGGACACGAATGGATGGCTCGTAATCGATCCTCTTCCCTCTTACGGTAGAGGCAGAGAACTCCCAGGTGGAAGGTATATTAGCTTTATACATGGAGACGGACTGGAAGATGTCGTCATTACAGGGCAAAATGGCACTATTGATGGCCAGGGTGAAGTTTGGTGGAATATGTGGAGAGATGGCACCTTGAAATACACGAGAGGCGGTCTAGTTGAGCTGATGAACTCAAAAGACGTTATAATATCGAATCTTGTTCTGCTGAATTCGCCTTTCTGGAATGTACATCCCATCTACTGCAGCAACGTTGTGGTCCGGGGATTGACGATTATAGCTCCGCGGGATTCGCCTAACACTGACGGAATCGATCCAGATTCCAGCTCGAATGTCTGCATCGAAGACTGCTATGTGAGCAACGGTGACGACGGGATAGCCATCAAGAGTGGGTGGGATGAGTACGGAATATCTTTCAACCGTCCGAGCTCCAACATTATTGTCCGGAGGATAACCATTTCAACGCCTTTCTCAGGAATAGCCATCGGCAGCGAGACGTCCGGCGGAATCCGCGACATTCTGGTAGAAAATATCAGCATCTACAGCTCGACAGTCGGAATCCGAGTCAAGACCAACGTAGGAAGAGGCGGGATCATTCGCAATATCACATTTTCCCACATATACCTGGACAATGTGGGCACTGGAATCAAATTTTCAGGCAATACGGGCGATCATCCCGATGCTCGATATAACCCTATGGCTCTTCCGGTCGTCGGAGATATCGCGGTGCTGAATGTGGTGGGCAATAGCATCAAAACCGCGGGTTCCCTGGTGGGATTGCAGCGTTCTCCATTTTCGAACATTTGTTTAGCAAACATTCATATTCAAGAAAAGGTTGGACGGTCAACTGGTTCTTCATGGGATTGCAAGGATGTGGAAGGAAGTTCCATTGGCGTAACTCCTAGCCCCTGTCAGCAACTTCAGAGCCCAGGATCTTCGTTCTGCAACAACGCTTTCCAACTTCATAATTTTGATCCATAA___ATTTTATTTTCCCCTCGGCTACTGGTCGAGTCCTGGGAATCATTTCTGAAATGAGCTGCAATTTTGAAATGCATTTCGTTATTTACATAATAATTATCTGTTGGTGTGAATGTGATGATGCAAGGGAATAAAACTTTTTGGAAAGTCAAAAAAGCTGTATTTGCTTACGCTAAATGTCAAATAAGTGATTAGATTAGTTAGCTGTGTCCCTACCACATGCTTTCCTTGTCTGGTAAAATAGACAGCCAAGCATGGCAAAAGTGGAAGGATGCTTATTGTTATATAGGCTAACACTAACAGTCAAGCCATGGTGGCCCATGGCTCTTAATTGCTATGAATGCGGGATAGCGCGTAAGTTTGTTTTTATTATCTTAAATGTCAATTCGTAAGGTCATTCACAACTTT

Fln Alignment:

Contig4986___IVPNKHRENVFSIRYFGAVGDGKTLNTKAFRTAISHIQNLNSRGKGGTLLYIPSGVWLTETFNLTSHLTLYLAKDAVLKASQDTNGWLVIDPLPSYGRGRELPGGRYISFIHGDGLEDVVITGQNGTIDGQGEVWWNMWRDGTLKYTRGGLVELMNSKDVIISNLVLLNSPFWNVHPIYCSNVVVRGLTIIAPRDSPNTDGIDPDSSSNVCIEDCYVSNGDDGIAIKSGWDEYGISFNRPSSNIIVRRITISTPFSG-IAIGSETSGGIRDILVENISIYSSTVGIRVKTNVGRGGIIRNITFSHIYLDNVGTGIKFSGNTGDHPDARYNPMALPVVGDIAVLNVVGNSIKTAGSLVGLQRSPFSNICLANIHIQEKVGRSTGSSWDCKDVEGSSIGVTPSPCQQLQ---SPGSSFCNNAFQL
B8LQW0______________VAPSCRAQSV-SIAEFGGVGDGTTVNTEAFRKAIEHLSEFSETGGGQ--LYIPPGRWLTGSFNLTDHFTLYLHKEAVILGSQDLEDWPLIPPLPSYGMGRDAPGPRYSSLINGYNLTDVVITGDNGTIDGQGAIWWQKFHKKILKNTRGYLVELMYSKDIIISNLTFLNSPAWNLHPVYSSNILIQYVTILAPLDSPNTDGIDPDSCSYVRIEDCYVVSGDDIVAIKSGWDEYGISFGMPSQHIVIRRLVGISPTSAIIALGSEMSGGIQDVRAEDIQAINSETGIRIKTSPGRGGFVKDIFVNRMTMVNMKWAFTMTGSYGSHPDNKYDPNALPAVERISYSNIVATNVSVAGKLDGIANAPFKDICLTNVTI-TMAAKSKKYPWNCTYIHGLSNAVYPQPCSLLEERPAEGDAFCPSPQEL

SNPs (tot: 14)

Position	A %	C %	G %	T %	Probability
1489	88		11		0.923564
1491		11	88		0.923564
1493			11	88	0.923564
1494		11		88	0.923564
1495		11	88		0.923564
1498	88		11		0.923564
1501		11		88	0.923564
1502	11			88	0.923564
1508	88	11			0.923564
1640		16		83	0.994512
1641	16	83			0.994512
1643			83	16	0.994512
1714		33		66	0.997452
1715	66	33			0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain