SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene4976

Length: 1997 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene4976
ATGGGGAAAGAATTGGGGAGAGAAGCCTTCTATTAAAAGCCATGGCAGGCACTTCTGCACTTTATCTTCTCTATCTTTAGCTGAACAGAGCGAAGCAAAAGAAGAAGAAGACAATGGACCGCCAATTCTCATTCTCTCCTGCAGACGATGTCGAAGGGAACTCCATGGCTGTAATAACCGAAGAAGAAGAAGAAAGCCCTGTGGTTTCAGGGCGAATGAGCCGTAGCGCCTCAGAATGGGCCTTCCAGAAGTTTCTCAGTTTTGATGGTTCCGAGATTCCGTCAGAAGATGGAGAAGGCGAACAGAAGCCTCTCGGTGTTAAAGATCCTCTGCTTCACGGTCATATGGACAACGTCTCTGCGGCGCTTAGTCCTCTCTTTGCCGAAGTCAAGGATGAGGTTCTTCTTCCTACAGACCCTCGAGATTACGAGACCTTCCTCAAGCGGAGGCTTAACCTTGCCTGCGCGGCAGTCGCCTTCACTCGGGTTACAGGAATTAGCTCTCCAGGCCTTGGTCCCTCAACAGTGGATGCAAACCAATCTCAGAACACTTTAGGATCAGAAAGAGCTGCATCATTGGTATCTCAATCTTCCGCCACAGATGCCAGAGCTATATGTGTTGGAGCTGCGAGCAGCACTGCTTCGGGACCAATTGGTATTCCAGCACTTCCTCCGCAGCCTAAAAGCGGAAATACTCAAGTGAGAACAACTAGTGGATCCTCAAGAGAGCAGTCAGATGATGATGACCAAGAAGTGGGACCCAGTGAACAAAGCATGGATCCCAGTCATGTGAAACGGGAGAGAAGAATGCTGTCAAATAGAGAGTCAGCAAGGCGATCAAGGAGAAGAAAACAAGCACACTTGACTGATCTTGAGATACAAGTTGCCCAGTTAAGAGTAGAAAACTCCTCTTTATTTAATCGCTTTACGGAGATCAATCAAAAGTACAATGAAGCTTCTGTTGATAATAGGGTACTGAAGTCTGATGTGGAAGCACTGAGAGCAAAGGTGAAGATGGCTGAATCTATGGTGCCCAAGGCAGTTGCTGCAGCAACTGCAGGGTTTCCACCTCAAAATTCATCACAGGTTCAATCAGCTGTCGGCCTACGATACAGAACTGGAATTTTTGACTCGCCTGCAACCAATGTCTTGCAAGGAGAGGATGGTCTTTACATGAATATAGCACAATCCACTGGAACCGGAGGTTCATCACTGACTTGTGCTATATCTGACATGAATCAACAGAACAAGGAATTGCAGATTCAACACACTGGGAGTAAAATGGGGCGTACACCCTCAATGCAGCGGGTTGCAAGCTTGGAACATTTACAAAAACGTATCCGTGGGGGAATGACTTGTGGATCCATGCCATGGGGTGGAGCTTGGGATGTTGATGGCTCACAAGTGATTGAGCACAGTGAACACTAACATGAATTCTGTATGACCATGAACCTCAAACTTCCAAGTCGTCTCTCTTCGGTGTCTAAAGTGTATTGTTTCACAACCTCTGGATTGACCCTATAAATCGATATCACATGCTAGCATAAACGGGCTATAATATTTTGAATGGTACATAGACGTGACATATTCATTGGATTGAGTTCAGGCGTACGGAATATATATGCTGTGTGCTTGTCTTGCACAAAAAATGCAAGACTAAGTATTTGCTAATGTTGCTTTTGTTCAAATGTACATATTTACCCTCATCATAACTTTGATGTGCATTGCTGGTTTTACGGAATTGTTGTAGCAGTCGGTTTCCTTCCGTACCCACTTAGCGGTGCGTGGGCTTCCTTTGGTTGAAAATGTTGAAGGTATCACATTAAAATCGAGCTACACATACATCAAGACTATTCGATGTACAGTCTAATTCCAAAAATAGTTAATAGGTAAGAGAAGAACCCCTGCTGGCATGACTCATGAGAAAGGGGTGAAAACACAACTGCGTCAGCCCATACTTGAATGAATGAATGCTTTTTAATAACGTCCAGGAGACCT

Ace file of the UniGene sp_v2.0_unigene4976 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	BZIP transcription factor n=2 Tax=core eudicotyledons RepID=Q93YM7_TOBAC	-	-	35%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	87%
Blast2go	light-inducible protein cprf2-like	-	-	56%

Source	GOs	Term	Type	Identity
Blast2go	regulation of transcription, DNA-dependent	GO:0006355	Biological Process	56%
Blast2go	sequence-specific DNA binding transcription factor activity	GO:0003700	Molecular Function	56%
Blast2go	sequence-specific DNA binding	GO:0043565	Molecular Function	56%
Blast2go	protein dimerization activity	GO:0046983	Molecular Function	56%
Blast2go	nucleus	GO:0005634	Cellular Component	56%

Source	InterPros	Term	Type	e value	Identity
Sma3	bZIP transcription factor, bZIP-1	IPR011616	-	0.0	-
Sma3	Basic-leucine zipper domain	IPR004827	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LQ62

Fln msg: your sequence is longer than subject: 438 - 357

Fln protein: MDRQFSFSPADDVEGNSMAVITEEEEESPVVSGRMSRSASEWAFQKFLSFDGSEIPSEDGEGEQKPLGVKDPLLHGHMDNVSAALSPLFAEVKDEVLLPTDPRDYETFLKRRLNLACAAVAFTRVTGISSPGLGPSTVDANQSQNTLGSERAASLVSQSSATDARAICVGAASSTASGPIGIPALPPQPKSGNTQVRTTSGSSREQSDDDDQEVGPSEQSMDPSHVKRERRMLSNRESARRSRRRKQAHLTDLEIQVAQLRVENSSLFNRFTEINQKYNEASVDNRVLKSDVEALRAKVKMAESMVPKAVAAATAGFPPQNSSQVQSAVGLRYRTGIFDSPATNVLQGEDGLYMNIAQSTGTGGSSLTCAISDMNQQNKELQIQHTGSKMGRTPSMQRVASLEHLQKRIRGGMTCGSMPWGGAWDVDGSQVIEHSEH*

Protein Length: 439

Fln nts: ATGGGGAAAGAATTGGGGAGAGAAGCCTTCTATTAAAAGCCATGGCAGGCACTTCTGCACTTTATCTTCTCTATCTTTAGCTGAACAGAGCGAAGCAAAAGAAGAAGAAGACA_-_ATGGACCGCCAATTCTCATTCTCTCCTGCAGACGATGTCGAAGGGAACTCCATGGCTGTAATAACCGAAGAAGAAGAAGAAAGCCCTGTGGTTTCAGGGCGAATGAGCCGTAGCGCCTCAGAATGGGCCTTCCAGAAGTTTCTCAGTTTTGATGGTTCCGAGATTCCGTCAGAAGATGGAGAAGGCGAACAGAAGCCTCTCGGTGTTAAAGATCCTCTGCTTCACGGTCATATGGACAACGTCTCTGCGGCGCTTAGTCCTCTCTTTGCCGAAGTCAAGGATGAGGTTCTTCTTCCTACAGACCCTCGAGATTACGAGACCTTCCTCAAGCGGAGGCTTAACCTTGCCTGCGCGGCAGTCGCCTTCACTCGGGTTACAGGAATTAGCTCTCCAGGCCTTGGTCCCTCAACAGTGGATGCAAACCAATCTCAGAACACTTTAGGATCAGAAAGAGCTGCATCATTGGTATCTCAATCTTCCGCCACAGATGCCAGAGCTATATGTGTTGGAGCTGCGAGCAGCACTGCTTCGGGACCAATTGGTATTCCAGCACTTCCTCCGCAGCCTAAAAGCGGAAATACTCAAGTGAGAACAACTAGTGGATCCTCAAGAGAGCAGTCAGATGATGATGACCAAGAAGTGGGACCCAGTGAACAAAGCATGGATCCCAGTCATGTGAAACGGGAGAGAAGAATGCTGTCAAATAGAGAGTCAGCAAGGCGATCAAGGAGAAGAAAACAAGCACACTTGACTGATCTTGAGATACAAGTTGCCCAGTTAAGAGTAGAAAACTCCTCTTTATTTAATCGCTTTACGGAGATCAATCAAAAGTACAATGAAGCTTCTGTTGATAATAGGGTACTGAAGTCTGATGTGGAAGCACTGAGAGCAAAGGTGAAGATGGCTGAATCTATGGTGCCCAAGGCAGTTGCTGCAGCAACTGCAGGGTTTCCACCTCAAAATTCATCACAGGTTCAATCAGCTGTCGGCCTACGATACAGAACTGGAATTTTTGACTCGCCTGCAACCAATGTCTTGCAAGGAGAGGATGGTCTTTACATGAATATAGCACAATCCACTGGAACCGGAGGTTCATCACTGACTTGTGCTATATCTGACATGAATCAACAGAACAAGGAATTGCAGATTCAACACACTGGGAGTAAAATGGGGCGTACACCCTCAATGCAGCGGGTTGCAAGCTTGGAACATTTACAAAAACGTATCCGTGGGGGAATGACTTGTGGATCCATGCCATGGGGTGGAGCTTGGGATGTTGATGGCTCACAAGTGATTGAGCACAGTGAACACTAA___CATGAATTCTGTATGACCATGAACCTCAAACTTCCAAGTCGTCTCTCTTCGGTGTCTAAAGTGTATTGTTTCACAACCTCTGGATTGACCCTATAAATCGATATCACATGCTAGCATAAACGGGCTATAATATTTTGAATGGTACATAGACGTGACATATTCATTGGATTGAGTTCAGGCGTACGGAATATATATGCTGTGTGCTTGTCTTGCACAAAAAATGCAAGACTAAGTATTTGCTAATGTTGCTTTTGTTCAAATGTACATATTTACCCTCATCATAACTTTGATGTGCATTGCTGGTTTTACGGAATTGTTGTAGCAGTCGGTTTCCTTCCGTACCCACTTAGCGGTGCGTGGGCTTCCTTTGGTTGAAAATGTTGAAGGTATCACATTAAAATCGAGCTACACATACATCAAGACTATTCGATGTACAGTCTAATTCCAAAAATAGTTAATAGGTAAGAGAAGAACCCCTGCTGGCATGACTCATGAGAAAGGGGTGAAAACACAACTGCGTCAGCCCATACTTGAATGAATGAATGCTTTTTAATAACGTCCAGGAGACCT

Fln Alignment:

Contig4976___MDRQFSFSPADDVEGNSMAVITEEEEE-SPVVSGRMSRSASEWAFQKFLSFDGSEIPSEDGEGEQKPLGVKDPLLHGHMDNVSAALSPLFAEV-----KDEVLLPTDPRDYETFLKRRLNLACAAVAFTRVTGISSPGLGPSTVDANQSQNTLGSE-RAASLVSQSSATDARAICVGAASSTASGPIGIPALPPQPKSGNTQVRTTXXXXXXXXXXXXXEVGPSEQSMDPSHVKRERRMLXXXXXXXXXXXRKQAHLTDLEIQVAQLRVENSSLFNRFTEINQKYNEASVDNRVLKSDVEALRAKVKMAESMVPKAVAAATAGFPPQNSSQVQSAVGLRYRTGIFDSPAT
B8LQ62______________MDRQFSFSPADDVEGNSMAVITEEEEEESPVVSGRMSRSASEWAFQKFLNYDGSEIPSEDGEGDHKTLGLKDPLPHGRMDNLSAPLSPLFGEVGDELRKDEVPPPTDPRDHQTFLKRRLNLACAAVAFTRVTGISSPGPGPSTVDANQSQNTLGSEGRAASLVSQSSATDARAIYIGAVSSTGSGPIGIPALPPKPKGGNTQVRTTSGSSREQSDDDDQEVGPSEQSMDPSHLKRVRRKLSNRESARRSRRRKQAHLNDLEIQVAQLRVENSSLFKRFTEINQKYSGASVDNRVLKSDVEALRAKVKMVESMVPK---AATAGFPFQNTSQVQSAVSLRYTTGIFDSPAT

SNPs (tot: 33)

Position	A %	C %	G %	T %	Probability
360	66		33		0.997452
703		14	85		0.993533
704		14	85		0.993533
705		14	71		0.984337
903		85		14	0.993533
1007		25		75	0.996473
1023	33		66		0.997452
1025	66		33		0.997452
1026		66		33	0.997452
1147		25		75	0.996473
1299	25	75			0.996473
1301		25		75	0.996473
1310		75		25	0.996473
1636		50	50		1.0
1657	88		11		0.923564
1718	22		77		0.999999
1720	77		22		0.999999
1767	77		22		0.999999
1798	66		33		1.0
1858	88	11			0.923564
1859		11	88		0.923564
1860	11		88		0.923564
1861	88			11	0.923564
1935	66	33			1.0
1936	66	33			1.0
1937	66	33			1.0
1938		33	66		1.0
1974	33		66		0.997452
1976	33	66			0.997452
1977		33		66	0.997452
1978		33		66	0.997452
1980	33			66	0.997452
1981	33	66			0.997452

SSRs (tot: 3)

Start position	End position	Sequence	Length
26	39	CATT CATT CATT CA	14
1804	1818	TTC TTC TTC TTC TTC	15
1887	1899	TCT TCT TCT TCT T	13

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain