SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene4700

Length: 2244 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene4700
ATCCACTGCAGAAGATCTGGACCGTTTTCATAAAGAATTGCAAATTTTATGCAAGTTGGATCATCCTAGCATAGCGAATTTGGTGGCAGCACATGCAAGACCCCCAAATTACATGTTTTTCTTCCCCTTCTATGAGAATGGTAACTTGGCTCAAGCTTTGCATGCTAAGGAATGGAATCCCTCCGTGGAGCAAATATTGACAATAATAATACAACTAGCCAGGGCTTTGCAGTACCTTCATAAGCTTGGAATTGTCCATAGGGATGTCAAACCTGCAAATATTCTTCTTGACAACAAATTGGAAGCACACCTTGCGGATTTTGGATTGGCAGTCTATGGAAAAGATCTTGAAAAGACAACTGTACAAAATTGGAAGTCATCTGGGAAACCAACTGGGGGTTTCCATAAAAAGAACATGGTTGGAACACTTATTTATATGGCACCCGAAGTACTCAAAAAAGAAATACATACTGAGAAGTCAGATATATATAGTTTTGCAATCACAATCAATGAACTTGTGACAGGTGTTGTTCCTTACACTGACCTTCAGACTGAGGCTCAGGCTCACACAGTACTAGAAATGACTTATACTGAACAACAACTTACAGCAGCTATTACCTCTGAAGGATTGCGCCCAGTACTTGCTGCTCCAGAGTCAGGGCTCCCATCCAGTCTATTGTCACTAATAAAGCTATGCTGGGATGACAATCCTGTGCAGCGCCCTTCCATTGATTTTTTGGTTAAAAGATTGGAATCAATTATTGCCCAATATAAGCATGAGGTACTAGAAAATAAGAACAGAGATGTTAATACTCTTTTGGATGCAAACCAACAGTGTCCAGCTCCATTTAACTATAATGATCAAAATCAAGTGGATATCAATTGGTCATATCAGGGTGCAATAACGGCAGAGAAAATCCAATCCGATGATCAGTTTTCTGTTAAGAAATGGATTAAAACTTTAGCCGAACATCCTGTGTATCATCCAACATTATCATGGGGTTCATTTGCCACTTGTGGTAGGCGAGAAATGATGGAGGACACACACTTTTTGTTGCCTCATTTGGGAAATGAGAAACATGCCTTTGCATTTGGGATCTTTGATGGTCATCGAGGTGCTGCAGCTGCACAGTTTGCAGTAAATGCCATACCAGGAATTTTGCATTCATTTGGTTCATTCCAAAGCCCATCAGAAGCATTGGTGAATGCATTTATTAGAACAGACATCTTGTTTCGTAATGAACTGGAATTGCATTGGTTATACAACAAAAGGTCAACGTAAGGATTGGCACCCTGGTTGCACTGCTGCAGTAGCATTGATTGTAGAAAACAGAGTTTTCGTAGCAAATGCTGGTGACTGTAGGACAATTTTGTGCAGAAAAGGACATGCAGTTGCTCTTAGTAAGGACCATGTTGCAAGCTGCATGGAAGAAAGAGATAGAGTCATCAAAGCAGGGGGACAAGTTAAATGGCAAGTAGATACTTGGCGTGTTGGTTCAGCTGCTCTCCAGGTTACACGTTCCATTGGTGACGACGATGTGAAGCCAGCAGTAACTGCGGAGCCAGAAATTACAGAGACTCTATTATCAACAGATGATGAATTCTTGGTGATGGCAAGTGATGGACTTTGGGATACAATAAGCAATGAAGAAGTGATATCTCTTATGAAAGATACTGTTAAAGAACCCGGCATGTGCTCTAAAAGACTTGCAACCACGGCCGTGGAAAGAGGGAGTAAAGATAATGTAACAGTAATTGTTATATTTCTTCGTCCAGTTTCAACTGTAGAGCGAATCTACTGAATATCACTATAAAGTCCGAGTTAGCGAGGAACACAAGGCTTTTTTGCTTTTCTTAAGTTGATTTGATCTGATAATTTGGTGTTCAGGTCAGGCAATGTCATTTTTTGATTCCTTGACGTACTCGTCGTTCTATGTCTCCCGTTTAATTTATAGAAATAAGAGTAAAATTATTGATGAGCATTTTACTAAAAGGATCTTGCCTTTATTGTTTTAATGGTTTGAATAACAATAATATCGGATTTATCATGCGGCGAGAGATATCAAAATCTGAACTTTTTCTCAGGTTTTGTACAAAATAAAGAGGTTTGTATCTTGAGAGATTTGATCCCTTTCAGTGTATGATAGATCCGGTGTTAAGATGGATTAGATGGATACAATGTCATGTTTTTAGAATTATTATTATGTATATGTTTGAGATAAACTTCATACTTTGATAAAATTT

Ace file of the UniGene sp_v2.0_unigene4700 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	putative protein phosphatase 2C 31 [Arabidopsis thaliana] sp\|Q940A2.1\|P2C31_ARATH RecName: Full=Protein kinase and PP2C-like domain-containing protein; Includes: RecName: Full=Probable serine/threonine protein kinase At2g40860; Includes: RecName: Full=Pro	-	-	55%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	32%
Blast2go	protein	-	-	72%

Source	ECs	Term	Type	e value	Identity
Blast2go	Transferases, Transferring phosphorous-containing groups, Protein-serine/threonine kinases.	EC:2.7.11.-	-	0.0	72%

Source	GOs	Term	Type	Identity
Blast2go	phosphorylation	GO:0016310	Biological Process	72%
Blast2go	transport	GO:0006810	Biological Process	72%
Blast2go	protein serine/threonine phosphatase activity	GO:0004722	Molecular Function	72%
Blast2go	protein serine/threonine kinase activity	GO:0004674	Molecular Function	72%

Source	InterPros	Term	Type	Identity
Sma3	Protein phosphatase 2C, manganese/magnesium aspartate binding site	IPR000222	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Protein phosphatase 2C-like	IPR001932	-	-
Sma3	Sugar transporter, conserved site	IPR005829	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3		IPR014045	-	-
Sma3	Protein phosphatase 2C	IPR015655	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NWY3

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 154 aas, putative chimeric sequence, No stop codon before M and low homology subject, your sequence is longer than subject: 390 - 281

Fln protein: MFFFPFYENGNLAQALHAKEWNPSVEQILTIIIQLARALQYLHKLGIVHRDVKPANILLDNKLEAHLADFGLAVYGKDLEKTTVQNWKSSGKPTGGFHKKNMVGTLIYMAPEVLKKEIHTEKSDIYSFAITINELVTGVVPYTDLQTEAQAHTVLEMTYTEQQLTAAITSEGLRPVLAAPESGLPSSLLSLIKLCWDDNPVQRPSIDFLVKRLESIIAQYKHEVLENKNRDVNTLLDANQQCPAPFNYNDQNQVDINWSYQGAITAEKIQSDDQFSVKKWIKTLAEHPVYHPTLSWGSFATCGRREMMEDTHFLLPHLGNEKHAFAFGIFDGHRGAAAAQFAVNAIPGILHSFGSFQSPSEALVNAFIRTDILFRNELELHWLYNKRST*

Protein Length: 391

Fln nts: ATCCACTGCAGAAGATCTGGACCGTTTTCATAAAGAATTGCAAATTTTATGCAAGTTGGATCATCCTAGCATAGCGAATTTGGTGGCAGCACATGCAAGACCCCCAAATTAC_-_ATGTTTTTCTTCCCCTTCTATGAGAATGGTAACTTGGCTCAAGCTTTGCATGCTAAGGAATGGAATCCCTCCGTGGAGCAAATATTGACAATAATAATACAACTAGCCAGGGCTTTGCAGTACCTTCATAAGCTTGGAATTGTCCATAGGGATGTCAAACCTGCAAATATTCTTCTTGACAACAAATTGGAAGCACACCTTGCGGATTTTGGATTGGCAGTCTATGGAAAAGATCTTGAAAAGACAACTGTACAAAATTGGAAGTCATCTGGGAAACCAACTGGGGGTTTCCATAAAAAGAACATGGTTGGAACACTTATTTATATGGCACCCGAAGTACTCAAAAAAGAAATACATACTGAGAAGTCAGATATATATAGTTTTGCAATCACAATCAATGAACTTGTGACAGGTGTTGTTCCTTACACTGACCTTCAGACTGAGGCTCAGGCTCACACAGTACTAGAAATGACTTATACTGAACAACAACTTACAGCAGCTATTACCTCTGAAGGATTGCGCCCAGTACTTGCTGCTCCAGAGTCAGGGCTCCCATCCAGTCTATTGTCACTAATAAAGCTATGCTGGGATGACAATCCTGTGCAGCGCCCTTCCATTGATTTTTTGGTTAAAAGATTGGAATCAATTATTGCCCAATATAAGCATGAGGTACTAGAAAATAAGAACAGAGATGTTAATACTCTTTTGGATGCAAACCAACAGTGTCCAGCTCCATTTAACTATAATGATCAAAATCAAGTGGATATCAATTGGTCATATCAGGGTGCAATAACGGCAGAGAAAATCCAATCCGATGATCAGTTTTCTGTTAAGAAATGGATTAAAACTTTAGCCGAACATCCTGTGTATCATCCAACATTATCATGGGGTTCATTTGCCACTTGTGGTAGGCGAGAAATGATGGAGGACACACACTTTTTGTTGCCTCATTTGGGAAATGAGAAACATGCCTTTGCATTTGGGATCTTTGATGGTCATCGAGGTGCTGCAGCTGCACAGTTTGCAGTAAATGCCATACCAGGAATTTTGCATTCATTTGGTTCATTCCAAAGCCCATCAGAAGCATTGGTGAATGCATTTATTAGAACAGACATCTTGTTTCGTAATGAACTGGAATTGCATTGGTTATACAACAAAAGGTCAACGTAAGGATTGGCACCCTGGTTGCACTGCTGCAGTAGCATTGATTGTAGAAAACAGAGTTTTCGTAGCAAATGCTGGTGACTGTAGGACAATTTTGTGCAGAAAAGGACATGCAGTTGCTCTTAGTAAGGACCATGTTGCAAGCTGCATGGAAGAAAGAGATAGAGTCATCAAAGCAGGGGGACAAGTTAAATGGCAAGTAGATACTTGGCGTGTTGGTTCAGCTGCTCTCCAGGTTACACGTTCCATTGGTGACGACGATGTGAAGCCAGCAGTAACTGCGGAGCCAGAAATTACAGAGACTCTATTATCAACAGATGATGAATTCTTGGTGATGGCAAGTGATGGACTTTGGGATACAATAAGCAATGAAGAAGTGATATCTCTTATGAAAGATACTGTTAAAGAACCCGGCATGTGCTCTAAAAGACTTGCAACCACGGCCGTGGAAAGAGGGAGTAAAGATAATGTAACAGTAATTGTTATATTTCTTCGTCCAGTTTCAACTGTAGAGCGAATCTACTGAATATCACTATAAAGTCCGAGTTAGCGAGGAACACAAGGCTTTTTTGCTTTTCTTAAGTTGATTTGATCTGATAATTTGGTGTTCAGGTCAGGCAATGTCATTTTTTGATTCCTTGACGTACTCGTCGTTCTATGTCTCCCGTTTAATTTATAGAAATAAGAGTAAAATTATTGATGAGCATTTTACTAAAAGGATCTTGCCTTTATTGTTTTAATGGTTTGAATAACAATAATATCGGATTTATCATGCGGCGAGAGATATCAAAATCTGAACTTTTTCTCAGGTTTTGTACAAAATAAAGAGGTTTGTATCTTGAGAGATTTGATCCCTTTCAGTGTATGATAGATCCGGTGTTAAGATGGATTAGATGGATACAATGTCATGTTTTTAGAATTATTATTATGTATATGTTTGAGATAAACTTCATACTTTGATAAAATTT

Fln Alignment:

Contig4700___SWGSFATCGRREMMEDTHFLLPHLGNEKHAFAFGIFDGHRGAAAAQFAVNAIPGILHSFGSFQSPSE-ALVNAFIRTDILFRN
A9NWY3______________SYGYASSPGKRSSMEDFFDTQICEVDGQIVGLFGVFDGHGGARAAEYVKQKLFANLISHPKFISDTKLAIADAYKQTDKEFLN

SNPs (tot: 40)

Position	A %	C %	G %	T %	Probability
804	88			11	0.923564
932		12		87	0.992554
933	12		87		0.992554
934		87		12	0.992554
978			25	75	0.996473
979	25			75	0.996473
987		25		75	0.996473
989	75		25		0.996473
994			20	80	0.995493
998	80	20			0.995493
999			20	80	0.995493
1000	20			80	0.995493
1002		80	20		0.995493
1085			83	16	0.994512
1086		83	16		0.994512
1088	83		16		0.994512
1089	83			16	0.994512
1091		16		83	0.994512
1092	16			83	0.994512
1095	16			83	0.994512
1100		20		80	0.995493
1102	20		80		0.995493
1104	80		20		0.995493
1282	14		85		0.993533
1601		25	75		0.996473
2035		14		85	0.993533
2044		12		87	0.992554
2134		85		14	0.993533
2135	85		14		0.993533
2136	14			85	0.993533
2140	83	16			0.994512
2141	16			83	0.994512
2142			16	83	0.994512
2143		16		83	0.994512
2144		16	83		0.994512
2145	16		83		0.994512
2146	16		83		0.994512
2151	16			83	0.994512
2152		16		83	0.994512
2156		16		83	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain