SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene4429

Length: 2245 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene4429
CGTCGTGACCTTGGCTGTCACTCAATTTTGACGTCGTGACCTTGGCTGTCACTCATTTTTCTGTCGGCATGTAGATCAATGTTTATTCCACGTTTCTGAATACTATAAATTGAGGAATAAGCCCCACAGGCATTTCACGAAGTCGAAGCAAGAATTTTCCTCAGCCAAGAGCGCGGAGAGAAGAGTAAGATTGTGTACAGAAGCGCCCAGGAAGAAAGAGGGCTGCAGCACAAAGGGCCACGGATTCAGAAGGATACAGGAAATGAGAGGAATGAAATTTCATTTGGGTTTAGTAATCACTGTAATATTTGCAATTAGTGTAAGTGTTAAGGGCCAACTGCATGATTTTTCTAATGGAGAGTTTGATGTGCGCCAATATCTCTCCACTTCATCCAGATATGGTGCTGGAAAAGAAGCAATGAATGATGATTATCAAATTTCTGCAGTGCCTCCCAATGGATGTACTCCAATTCTTTTAAATCTTGTGGCCAGGCATGGAACTCGTGCACCGACCAAGAAGCGCATAAAGGAGCTAGTGCAATTATCTATACGTTTGGAGAGTCTTTTGAAGTCTGCAGATGATACACTTACAGGGGAAACACATGGTTCACAACAAATACCATCATGGCTGAGGGGCTGGCAATGTCCCTGGGACATAAAGAAAACAAATGGAGAATTAACACGTGAAGGTGAGGAAGAGTTGTATAATCTGGGAATGAGGTTAAGGGAAAGGTTTCCCCAGATATTTGATGAAGATTATCATCCTGATGTTTACTTGATAAGTGCAACTCAGGTCCCTCGTGCTTCAGCTAGTGCAGTTGCATTTGGCATGGGGCTTTTTGCTGGGAAAGGAGTTCTTGGAGCAGGCCAGCATCGTGCATTTGCTGTTATAACTGACAGTCGAGCAAATGATATCCATTTACGATTTCATGATACTTGCCAAACATACAAGGAGTTCAAGAAGAAGCGCAAGCCTGCTTTAAAAGGATTACTATTTCCGGTTTATGCTGAAGTTTCAGCTTCATTGGTAAAACGCTACAATCTGAACTTTACAAAAGATGATGTGGCTTTACTTTGGTTTCTTTGCAGGCAGGAAGCAGCTTTACTAGACAAAACTGATCAAGCGTGTGGACTTTTTACCCAAATGGAGGTTGAACTACTTGAATGGGTTGATGATTTGGTGCTACATTATCTTAAAGGATATGGAGAATCAATAAATTATCAGATGGGGGTACCATTACTACAAAATATTGTGCAGTCAATGGAGCAAGCTATTTTAATGAAAAATTCCAGCAGCTGTGGAGAAGGCTCGGTTACGATTTGCTCATGCAGAGACCATCATACCGTTCTCTTGTCTCCTTGGTCTATTTCTTTCTGCCTCTGATACAGAAAGAATTCAAAGGGAGCAGCCTTTGGAGCCTCCACCAAAACCCCCTCAAGAGCGGAGATGGAGAGGAAGTACAGTTGCACCTTTTGCAGGAAACAACATGCTTGTCCTATACAAATGCCCAGTCCAAGGGGGTAGTACTGGAAAGGTTATGGCGCCAGAAGAACCGAGTAACAGCTTTTTTGTTCAAATTTTACACAATGAAAAGCCAGTCTCTATTCCGGCTTGCAATGGAACTTATTTTTGCCCATTTGAAGATTTTAAGGAAAGAGTTGCAGGACCACATCTGAGACACACATTTGAATCATTGTGTACTGTTAAGGTTAAAAATGCAGATTGCCCCTTAACTTGCAGGATATCTAGGTTTTTCCAGTGGTTATTTCTAAGAAAGGGATACAAGCTCCATTCTTATGAAGATAAAGATGAGTTGTGATAGTTTCATTTCATGTTTTAGGAAGTTTTGAACTTGAAATTATTTTTGTTAATCTTAATTGCCATTTTATCACCTTAATCTCAACTAGTAATATTGCTAAAAACATTACGAAATATTAAGTGTTGTTCCATAACTTCGACAGAGAGATTTTGTGCACACTTAATTTCAGCATTGCTCCGGAACCGTGGAGTTAGCATGCATTGCTTTACAACGATATGTTACATGAGTTAGATAAGATTGTGATGGGGCTTTGAAAGCATGACATTGTTAGCGATTTTACTGTTCGTCTTTACGTCTTAAACTAGCATATAAATATCTTTGGGATATATTCATTAGCCTAAGAAACATGTAGTTAGTTGCGTGGATTGTAAATGTTTTTACTGTATGTTTTTCTTTTATGTGAATAATGTAGAGTAATGTGTTAG

Ace file of the UniGene sp_v2.0_unigene4429 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	pfam00328, His_Phos_2, Histidine phosphatase superfamily (branch 2)	-	-	6.0e-32	26%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	93%
Blast2go	protein	-	-	0.0	70%

Source	ECs	Term	Type	e value	Identity
Blast2go	Hydrolases, Acting on ester bonds, Phosphoric monoester hydrolases.	EC:3.1.3.-	-	0.0	70%

Source	KEGGs	Term	Identity
Blast2go	Fructose and mannose metabolism	00051	70%
Blast2go	Ascorbate and aldarate metabolism	00053	70%
Blast2go	Lipopolysaccharide biosynthesis	00540	70%
Blast2go	Ether lipid metabolism	00565	70%
Blast2go	Sphingolipid metabolism	00600	70%
Blast2go	Thiamine metabolism	00730	70%
Blast2go	Riboflavin metabolism	00740	70%
Blast2go	Nicotinate and nicotinamide metabolism	00760	70%
Blast2go	Metabolic pathways	01100	70%
Blast2go	Biosynthesis of secondary metabolites	01110	70%

Source	GOs	Term	Type	e value	Identity
Blast2go	phosphatase activity	GO:0016791	Molecular Function	0.0	70%

Source	InterPros	Term	Type	e value	Identity
Sma3	Histidine phosphatase superfamily, clade-2	IPR000560	-	0.0	-
Sma3	Histidine acid phosphatase, eukaryotic	IPR016274	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ACK4

Fln msg: Warning!, your query overlaps and the subject is separated, your sequence is longer than subject: 520 - 228

Fln protein: MRGMKFHLGLVITVIFAISVSVKGQLHDFSNGEFDVRQYLSTSSRYGAGKEAMNDDYQISAVPPNGCTPILLNLVARHGTRAPTKKRIKELVQLSIRLESLLKSADDTLTGETHGSQQIPSWLRGWQCPWDIKKTNGELTREGEEELYNLGMRLRERFPQIFDEDYHPDVYLISATQVPRASASAVAFGMGLFAGKGVLGAGQHRAFAVITDSRANDIHLRFHDTCQTYKEFKKKRKPALKGLLFPVYAEVSASLVKRYNLNFTKDDVALLWFLCRQEAALLDKTDQACGLFTQMEVELLEWVDDLVLHYLKGYGESINYQMGVPLLQNIVQSMEQAILMxxIPAAVEKARLRFAHAETIIPFSCLLGLFLSASDTERIQREQPLEPPPKPPQERRWRGSTVAPFAGNNMLVLYKCPVQGGSTGKVMAPEEPSNSFFVQILHNEKPVSIPACNGTYFCPFEDFKERVAGPHLRHTFESLCTVKVKNADCPLTCRISRFFQWLFLRKGYKLHSYEDKDEL*

Protein Length: 521

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTTGACGTCGTGACCTTGGCTGTCACTCATTTTTCTGTCGGCATGTAGATCAATGTTTATTCCACGTTTCTGAATACTATAAATTGAGGAATAAGCCCCACAGGCATTTCACGAAGTCGAAGCAAGAATTTTCCTCAGCCAAGAGCGCGGAGAGAAGAGTAAGATTGTGTACAGAAGCGCCCAGGAAGAAAGAGGGCTGCAGCACAAAGGGCCACGGATTCAGAAGGATACAGGAA_-_ATGAGAGGAATGAAATTTCATTTGGGTTTAGTAATCACTGTAATATTTGCAATTAGTGTAAGTGTTAAGGGCCAACTGCATGATTTTTCTAATGGAGAGTTTGATGTGCGCCAATATCTCTCCACTTCATCCAGATATGGTGCTGGAAAAGAAGCAATGAATGATGATTATCAAATTTCTGCAGTGCCTCCCAATGGATGTACTCCAATTCTTTTAAATCTTGTGGCCAGGCATGGAACTCGTGCACCGACCAAGAAGCGCATAAAGGAGCTAGTGCAATTATCTATACGTTTGGAGAGTCTTTTGAAGTCTGCAGATGATACACTTACAGGGGAAACACATGGTTCACAACAAATACCATCATGGCTGAGGGGCTGGCAATGTCCCTGGGACATAAAGAAAACAAATGGAGAATTAACACGTGAAGGTGAGGAAGAGTTGTATAATCTGGGAATGAGGTTAAGGGAAAGGTTTCCCCAGATATTTGATGAAGATTATCATCCTGATGTTTACTTGATAAGTGCAACTCAGGTCCCTCGTGCTTCAGCTAGTGCAGTTGCATTTGGCATGGGGCTTTTTGCTGGGAAAGGAGTTCTTGGAGCAGGCCAGCATCGTGCATTTGCTGTTATAACTGACAGTCGAGCAAATGATATCCATTTACGATTTCATGATACTTGCCAAACATACAAGGAGTTCAAGAAGAAGCGCAAGCCTGCTTTAAAAGGATTACTATTTCCGGTTTATGCTGAAGTTTCAGCTTCATTGGTAAAACGCTACAATCTGAACTTTACAAAAGATGATGTGGCTTTACTTTGGTTTCTTTGCAGGCAGGAAGCAGCTTTACTAGACAAAACTGATCAAGCGTGTGGACTTTTTACCCAAATGGAGGTTGAACTACTTGAATGGGTTGATGATTTGGTGCTACATTATCTTAAAGGATATGGAGAATCAATAAATTATCAGATGGGGGTACCATTACTACAAAATATTGTGCAGTCAATGGAGCAAGCTATTTTAATGxxxxxxATTCCAGCAGCTGTGGAGAAGGCTCGGTTACGATTTGCTCATGCAGAGACCATCATACCGTTCTCTTGTCTCCTTGGTCTATTTCTTTCTGCCTCTGATACAGAAAGAATTCAAAGGGAGCAGCCTTTGGAGCCTCCACCAAAACCCCCTCAAGAGCGGAGATGGAGAGGAAGTACAGTTGCACCTTTTGCAGGAAACAACATGCTTGTCCTATACAAATGCCCAGTCCAAGGGGGTAGTACTGGAAAGGTTATGGCGCCAGAAGAACCGAGTAACAGCTTTTTTGTTCAAATTTTACACAATGAAAAGCCAGTCTCTATTCCGGCTTGCAATGGAACTTATTTTTGCCCATTTGAAGATTTTAAGGAAAGAGTTGCAGGACCACATCTGAGACACACATTTGAATCATTGTGTACTGTTAAGGTTAAAAATGCAGATTGCCCCTTAACTTGCAGGATATCTAGGTTTTTCCAGTGGTTATTTCTAAGAAAGGGATACAAGCTCCATTCTTATGAAGATAAAGATGAGTTGTGA___TAGTTTCATTTCATGTTTTAGGAAGTTTTGAACTTGAAATTATTTTTGTTAATCTTAATTGCCATTTTATCACCTTAATCTCAACTAGTAATATTGCTAAAAACATTACGAAATATTAAGTGTTGTTCCATAACTTCGACAGAGAGATTTTGTGCACACTTAATTTCAGCATTGCTCCGGAACCGTGGAGTTAGCATGCATTGCTTTACAACGATATGTTACATGAGTTAGATAAGATTGTGATGGGGCTTTGAAAGCATGACATTGTTAGCGATTTTACTGTTCGTCTTTACGTCTTAAACTAGCATATAAATATCTTTGGGATATATTCATTAGCCTAAGAAACATGTAGTTAGTTGCGTGGATTGTAAATGTTTTTACTGTATGTTTTTCTTTTATGTGAATAATGTAGAGTAATGTGTTAG

Fln Alignment:

Contig4429___HYLKGYGESINYQMGVPLLQNIVQSMEQAILMKxxxKIPAAVEKARLRFAHAETIIPFSCLLGLFLSASDTERIXXXXXXXXXXXXXXXXRWRGSTVAPFAGNNMLVLYKCPVQGGSTGKVMAPEEPSNSFFVQILHNEKPVSIPACNGTYFCPFEDFKERVAGPHLRHTFESLCTVKVKNADCPLTCRISRFFQWLFLRKGYKLHSYEDKDEL
D5ACK4______________HYLKGYGESINYRMGVPLLQNILQSMEQAILMKxxxQIPAAVEKARLRFAHAETIIPFTCLLGLFLSASDIERIQSEQPLEPPSKPPQQRRWRGSTVAPFAANTMLVLYKCPAQEGSTGKVMVPEELTNSFFVQILHNEEPVSIPGCNGSYFCPFEVFKERVAGPHLRHTFESLCTVKVTHADCPLTCRISRFFQWLFLRKGYAPHSCEDKDEL

SNPs (tot: 33)

Position	A %	C %	G %	T %	Probability
885		66		33	0.997452
1197	90	10			0.90298
1198		10		90	0.90298
1245	87	12			0.992554
1273		50	50		1.0
1338		75		25	0.996473
1367	75			25	0.996473
1390	25			75	0.996473
1392		25	75		0.996473
1394	25	75			0.996473
1395	25			75	0.996473
1397		75	25		0.996473
1402	75	25			0.996473
1407			25	75	0.996473
2142	53		7	38	1.0
2146	15	84			0.999986
2173	86		13		0.999973
2178	86	13			0.999973
2181	13			86	0.999973
2191		14		85	0.999981
2207	88	11			0.923564
2208		44	55		1.0
2209		44		55	1.0
2210	33	11	55		1.0
2211	66	33			1.0
2212		88		11	0.923564
2215		62		37	1.0
2216	37	62			1.0
2217	62	37			1.0
2218	62			37	1.0
2219			62	37	1.0
2222		62	37		1.0
2226	57			42	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain