SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene4407

Length: 1703 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene4407 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	lanatoside 15'-O-acetylesterase [Digitalis lanata]	-	-	61%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	67%
Blast2go	gdsl esterase lipase	-	-	74%

Source	GOs	Term	Type	Identity
Blast2go	lipid metabolic process	GO:0006629	Biological Process	74%
Blast2go	hydrolase activity, acting on ester bonds	GO:0016788	Molecular Function	74%
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	74%

Source	InterPros	Term	Type	Identity
Sma3	Lipase, GDSL	IPR001087	-	-
Sma3	Histone H2A	IPR002119	-	-
Sma3	Lipase, GDSL, active site	IPR008265	-	-
Sma3	Aldehyde dehydrogenase, conserved site	IPR016160	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9P0U3

Fln msg: your sequence is longer than subject: 397 - 326

Fln protein: MWAMASTAIERKVFHLEKWIVCGAAVVGLMIFCQVESSQGKCAFPAIFNFGDSNSDTGGFYAAFPAENPPYGMTFFHKPVGRASDGRLVIDFIAQAFGLPFLSPYLQSLGSDFRHGANYASDAGTVLQPNTSQYVTGISPFYLGVQYREMLDFKVRVLDLLSEGKKLEYLPNPEVFSQGLYTLDIGQNDFTSKLGEIGIQGVKQFLPQVASQIGETVKALYKEGARTIFVANLAPIGCFPSFLTELPHNQSDLDSYGCMISYNNAVVDYNNLLREQLEEVRKVLQDASVIYVDSHAIKLEIFTNPTKHGFKYGTKACCGTGGDYNFSPEVFCSQSKTLNGIVVTASACSDPSGYVSWDGIHNTDAANIYIANEILSGKYFQPPFPLSKLCDLQPIG*

Protein Length: 398

Fln nts: CGTCGTGACCTTGGCTGTCACGTCGGGAAATTTGAGAGTTTTTCATTCTTATGCTGCGGTAAGCTTACAGGAAA_-_ATGTGGGCAATGGCTAGCACGGCCATCGAAAGAAAAGTATTCCATCTAGAGAAATGGATTGTTTGTGGTGCAGCAGTTGTGGGGCTGATGATATTCTGTCAAGTAGAAAGCAGCCAAGGGAAATGTGCATTTCCTGCAATTTTCAATTTCGGAGACTCGAATTCAGATACAGGTGGCTTCTATGCTGCTTTCCCTGCAGAAAATCCTCCCTATGGAATGACTTTCTTCCATAAGCCTGTAGGTCGTGCTTCTGATGGACGCCTTGTGATTGATTTCATAGCTCAAGCTTTCGGCCTGCCTTTTCTTAGTCCATATCTGCAGTCATTAGGCTCTGATTTTCGTCATGGGGCCAACTATGCCTCGGATGCTGGTACTGTTCTTCAACCAAATACTTCACAATATGTTACCGGAATCAGCCCTTTCTACTTGGGAGTTCAGTACAGGGAGATGCTGGATTTCAAGGTTCGAGTTCTTGATCTTCTTTCAGAAGGAAAAAAATTGGAGTATCTACCAAACCCTGAGGTATTCAGTCAAGGTCTCTACACACTTGATATTGGGCAGAATGATTTCACCTCAAAACTTGGTGAAATAGGAATTCAAGGAGTGAAGCAGTTTCTTCCCCAGGTAGCTTCACAGATTGGTGAAACTGTTAAGGCATTGTATAAAGAAGGGGCAAGGACAATATTTGTGGCAAATCTTGCACCAATCGGTTGCTTTCCTTCATTTCTGACTGAACTTCCTCATAATCAATCTGATCTGGACAGTTATGGATGTATGATATCATACAACAATGCAGTGGTGGACTATAATAACCTACTTAGGGAGCAGCTTGAAGAAGTTCGAAAGGTCTTACAGGATGCATCTGTTATATATGTTGATTCACATGCGATCAAGTTGGAGATTTTTACAAATCCAACGAAACATGGGTTCAAGTATGGAACAAAGGCCTGCTGTGGCACTGGAGGTGACTACAATTTTAGTCCAGAGGTTTTCTGCAGCCAAAGCAAGACATTAAATGGTATTGTTGTCACAGCTTCAGCATGTTCTGATCCATCAGGTTACGTGTCCTGGGATGGAATTCATAACACTGATGCTGCAAATATATATATAGCCAATGAAATTCTCAGTGGTAAATACTTTCAGCCACCATTTCCACTCAGCAAACTTTGTGATCTCCAACCTATTGGGTGA___ACTTGAGGACGAAAAGAAGCTCCATATGCTATGGAAAAAATGTATTCAATAAAGCCTTCACTTCCAAGGCCTCTCAATTCAATAGAGTTACTGATAAGCAGCATGGGCTTTGTCAATTATTAAGCCATCATGAGTTTTGGCTCTTTTTCAATTAGTCGTATTCTCTCACTCACATAATATTAATGTGCTTGGGTCATGTTTAATTCACCCTTATGGTGACCATTTGATCAATATTAAAAAATCAATTGTATCCAAGGAGGATGGGAACAAATGTTTTCAGATTGAATCAATGTCAGTGCTGTTGGGTATGATTCTTGTTGCTCATTTGGGTAGGCAGATTCAGTTGAACTTCTAAAACAAATCTGAGATAACTTGCATTTGTAAACTCTTCCAAATATTTTTATAAATGAAATTTTGTGAGATATTGTTGATTCCAAA

Fln Alignment:

Contig4407___MWAMASTAIERKVFHLEKWIVCGAAVVGLMIFCQVESSQGKCAFPAIFNFGDSNSDTGGFYAAFPAENPPYGMTFFHKPVGRASDGRLVIDFIAQA--FGLPFLSPYLQSLGSDFRHGANYASDAGTVLQPNTSQYVTGISPFYLGVQYREMLDFKVRVLDLLSEGKKLEYLPNPEVFSQGLYTLDIGQNDFTSKLGEIGIQGVKQFLPQVASQIGETVKALYKEGARTIFVANLAPIGCFPSFLTELPHNQSDLDSYGCMISYNNAVVDYNNLLREQLEEVRKVLQDASVIYVDSHAIKLEIFTNPTKHGFKYGTKACCGTGGDYNFSPEVFCSQSKTLNGIVVTASACSDPSGYVSWDGIHNTDAANIYIANEILSGKYFQPPFPLSKLCDLQPIG
A9P0U3______________MWVIESMTIERKIFHVEKWIVCAAAVVGLMIFCQVESSQGKC--------------------AFPA--------IFNFGDSNSDTGGFYAAFPAESPPYGMTF-----------FNKPAGRASDG------------------------RLVVDFL---------GKNLEYLPTPEVFSQALYTLDIGQNDFTSRLGEIGIQGVKQFLPQVASQIGETVKALYGEGARTIFVANLAPIGCFPSFLTELPHNQSDLDSYGCMISYNSAVVDYNNLLREKLEEVRKVLPNASVIYVDSHAIKLEIFTNPTKHGFKYGTKACCGTGGDYNFSPQVFCSQSKKLNGTVVTASACSDPSSYVSWDGVHNTDAANIYIANEILSGKYFQPPFPLSTLCDLQPIG

SNPs (tot: 33)

Position	A %	C %	G %	T %	Probability
221	25	75			0.996473
223		75	25		0.996473
224		75		25	0.996473
504		88		11	0.923564
567	90		10		0.90298
568	10	90			0.90298
571	90		10		0.90298
574		90	10		0.90298
575		10		90	0.90298
576		10	90		0.90298
578	10			90	0.90298
585		10	90		0.90298
586	90		10		0.90298
589	90		10		0.90298
1072	11		88		0.923564
1594	10	90			0.90298
1595	10			90	0.90298
1597			10	90	0.90298
1617	25		75		1.0
1648			80	20	1.0
1649		80		20	1.0
1663	75	8	16		0.99999
1664	75	8		16	0.99999
1665	83		16		0.999991
1669	18	81			0.999994
1670			18	81	0.999994
1672	80	20			0.999996
1675			25	75	1.0
1676			25	75	1.0
1679	40	60			1.0
1681			50	25	0.995533
1682	50	25	25		0.995533
1683	50	50			1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain