SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene4310

Length: 1690 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene4310 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Sur2 hydroxylase/desaturase, putative n=1 Tax=Ricinus communis RepID=B9SM94_RICCO	-	-	72%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Blast2go	sur2 hydroxylase	-	-	86%

Source	ECs	Term	Type	e value	Identity
Blast2go	Methylsterol monooxygenase.	EC:1.14.13.72	-	0.0	86%

Source	KEGGs	Term	Identity
Blast2go	Steroid biosynthesis	00100	86%
Blast2go	Biosynthesis of alkaloids derived from terpenoid and polyketide	01066	86%
Blast2go	Metabolic pathways	01100	86%

Source	GOs	Term	Type	Identity
Blast2go	fatty acid biosynthetic process	GO:0006633	Biological Process	86%
Blast2go	sphingoid biosynthetic process	GO:0046520	Biological Process	86%
Blast2go	photomorphogenesis	GO:0009640	Biological Process	86%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	86%
Blast2go	sphingosine hydroxylase activity	GO:0000170	Molecular Function	86%
Blast2go	iron ion binding	GO:0005506	Molecular Function	86%
Blast2go	C-4 methylsterol oxidase activity	GO:0000254	Molecular Function	86%
Blast2go	Golgi apparatus	GO:0005794	Cellular Component	86%
Blast2go	integral to membrane	GO:0016021	Cellular Component	86%
Blast2go	endoplasmic reticulum	GO:0005783	Cellular Component	86%

Source	InterPros	Term	Type	e value	Identity
Sma3	Fatty acid hydroxylase	IPR006694	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A9A2

Fln msg:

Fln protein: MVFWEGYVSDETMGALAPIVVYWIYAGAYQMLPPLDRYRMHSRKEEELRNLVSLPTVIKGVLLQQLVQATVALSLFSMTSKVGLTGKSLQPSFLVQIFQVLIAMFVMDTWQYFVHRYMHQNKFLYRHVHSQHHKLVVPYAIGALYNHPLEGLLLDTLGGAISFLVSGMTAQTAVFFFTFAVIKTIDDHCGLWLPGNIFHILFQNNTAYHDIHHQLQGTKYNYSQPFFVLWDKLLGTYMPYTLEKRPDGGFEARLLKE*

Protein Length: 259

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATCAGGTTTTTTCTGTTAATGATATCTATGAGACGGAGACAGAGAGATCTGGCAAAGAGAAACGTTTGGAAAATAAATTAAAGAACATCGAATTCCGGCAATTGACGTGATTTATCGTTCGTCTGGTCTCGTCCTTCTCCCGCTTTGTGTGGATTGAGCCAGTCAGTGCTGTTGGTCGATCCCATAGATCTGGAGCTGATGATGCCCTGAAAGGCGGACGGAAGAAGCAAAACCCAACTCAACTTTACTGAGCAAAACAGCAATGTGGGGTTGTAGCTTCGAATCTCAGCCGCATTTACATTAATCCGTCCGTTATCTTCTCTTGTTGGGGCACTTGAAGGAAAGAAAGT_-_ATGGTTTTCTGGGAGGGATATGTGAGCGATGAAACAATGGGGGCGCTGGCGCCGATTGTTGTGTACTGGATATATGCGGGAGCTTATCAGATGTTACCGCCGTTAGATCGTTATCGGATGCATTCTCGCAAGGAGGAGGAGCTTCGTAATCTGGTGTCTCTTCCCACTGTAATTAAAGGCGTTCTTCTGCAGCAGCTCGTTCAGGCCACCGTTGCGCTTTCGCTATTTTCGATGACATCAAAGGTGGGTCTAACTGGGAAGTCATTGCAACCATCATTTCTCGTTCAGATTTTTCAAGTGTTAATTGCAATGTTCGTAATGGATACTTGGCAGTACTTTGTGCACCGCTACATGCATCAGAACAAGTTTCTATATCGTCATGTTCATTCACAACACCACAAGCTGGTAGTGCCTTATGCCATTGGTGCTTTATACAATCATCCACTTGAGGGGCTTCTGCTTGATACTCTAGGTGGCGCAATTTCATTTCTTGTCTCAGGAATGACAGCACAGACTGCTGTGTTTTTTTTTACATTTGCGGTTATTAAAACCATCGATGATCATTGTGGGCTTTGGTTACCCGGGAATATATTCCACATACTGTTTCAAAATAATACTGCTTATCATGATATCCACCATCAGCTTCAAGGGACCAAGTATAATTATTCACAACCCTTTTTTGTGTTGTGGGATAAGCTTCTTGGCACTTATATGCCATACACCCTTGAAAAGCGACCTGATGGAGGCTTTGAAGCAAGACTACTTAAGGAATAA___TCTTTTTTCTAGAGTGCAAGGATTTTGACAGTGTCACAAGGTGACGGTGGATATCCAAGTGTCATATGCAATCTCTGCCAGGCATTTGAAACTTAGCTCATGTGCACTTACTGAAATTGGGGCAGTCACAAGAAAATACAGTGCTGTTTTCAGATTCTACTTGCCTTCTAAATATCAGAGGAAAATGGCTGTGACTGAGAGGGTAACCTACACTCATAGTTGTCTACTGCTTGTCACAGTGTACCACTGTTATAAGTACGATTTCAGTTTCTTTAGGAAAATGCAGTGTATTATGATGTTTTTCCATTGTGTGAGTTAATGATGAGCATTATTTTTTATATTTTTCATTCCTTATTAACGTGCTAGTTGGATAGCAATTGGCCATCAGTGGCATGAATAGACGTATTAAACTTGATGTTGTTTATATCTATTTAATGGCCAGAACTCATTCTATGTATAATCTTCACTTGAATGAAGAGAAGTGCTTTGTATTTTGGCTATCGGGGTGATATTTCTTCTGTACCTAAGCCAATTCTTACATA

Fln Alignment:

Contig4310___MGALAPIVVYWIYAGAYQMLPPLDRYRMHSRKEEELRNLVSLPTVIKGXXXXXXXXXXXXXXLFSMTSKVGLTGKSLQPSFLVQIFQVLIAMFVMDTWQYFVHRYMHQNKFLYRHVHSQHHKLVVPYAIGALYNHPLEGLLLDTLGGAISFLVSGMTAQXXXXXXXXXVIKTIDDHCGLWLPGNIFHILFQNNTAYHDIHHQLQGTKYNYSQPFFVLWDKLLGTYMPYTLEKRPDGGFEARLLKE
D5A9A2______________MGALAPIVVYWIYAGAYQMLPRLDRYRMHSRKEEELRNLVSLPTVIKGVLLQQLVQATVALSLFAMTSKVGLTGKSLQPSFLVQICQALIAMLVMDTWQYFVHRYMHQNTFLYRHVHSQHHRLVVPYAIGALYNHPLEGLLLDTLGGAISFLVSGMTAQTAVFFFSFAVIKTVDDHCGLWLPGNIFHALFQNNTAYHDIHHQLQGTKYNYSQPFFVMWDKLLGTHMPYTLEKRPDGGFEARPLKE

SNPs (tot: 37)

Position	A %	C %	G %	T %	Probability
868	11	88			0.923564
870	11	88			0.923564
871		88		11	0.923564
1021		16		83	0.994512
1085			87	12	0.992554
1087	12	87			0.992554
1204	20	80			0.995493
1205		80	20		0.995493
1208		20		80	0.995493
1210	80		20		0.995493
1212	20		80		0.995493
1214			20	80	0.995493
1215		80	20		0.995493
1217	80	20			0.995493
1221	20		80		0.995493
1264	75			25	0.996473
1265	75		25		0.996473
1266	25			75	0.996473
1267		66		33	0.997452
1269	33		66		0.997452
1275	33		66		0.997452
1276		66	33		0.997452
1278		66		33	0.997452
1280	33	33			0.997753
1282		66	33		0.997452
1284		33		66	0.997452
1285			33	66	0.997452
1531		10	90		0.999898
1532		15	85		1.0
1533	80	15		5	1.0
1534		10	85		0.999793
1536	85	5		10	0.999793
1541	11		88		0.999937
1563	84	15			0.999986
1564		84		15	0.999986
1568	88	11			0.923564
1569	88			11	0.923564

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain