SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene4181

Length: 2485 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene4181
CGTCGTGACCTTGGCTGTCATGGGAATCCTCTTTCTCTTACAGGGCTTGGATGATCAAAGCGTTCACTCGCCCAGGTGGAGAGTTTCTAACTGCTCTGCTCTGCTCTGTTCACTCTTATGTGGATCTGCAATTTGTCCCCGAGAGCAGATCGAGTATAGTTTCATTAGGTTCGTTTGTTGCAGTATATGAAGCGGTTTTCATCCCTACAAAGGTCTGTTTTTCCATAGAAAAAGTTGCATTTTCTTACATTTTTATGAAGTGATTTGTGAGCTCTAGGAAGCAGCTTTCTGCATTGACCAGGATTTGGTATTTAGTTTCTATTTGGCCAAGTTGTCGAATCGCAATCAAGGTTGAAAAGGCCTTTCGGACCATATATTTCATGCGTCTAGGGACCTTATTAATGAAGAAAGAAAACCATAGGTTATAGTTGTGATAAACTATAGATTAACCTAGTACCATTATTTGGCAGTCCGTTTATTTAATCTGAAAAAATGGGGTCCTGCAAAGAATGGAGTGAGGAAAAAATGACATCGCGAAATGCCCTTGAAGGTCTGATGCCTGCAAAACTAAGGCAAATGGTAGGAAAAAAATTTCAAGCCATGAAAGTGGAAAGTCTTCTTCACACTAGATTGCTCACATACTTGGGTTATGCGGTTGCACTCATTTTACTTTTCTCATATTCCTGGTACGTGGGGAATCTGATCACAATGGACAACTTGTATGCTCTGTGGAAGGCCGGCAAGGTGCACAAAAATAGTCTTATAGTGTTATTTTGGGTGGTGGCATGCCTGGGTGTTGTTTGGTGTATGTCTAGGCCAAGAAAAATCTACATGCTGGATTTCGCCTGCTTCAAAGCAGCGGAAGATCGAAAATGCACCCTTGAACTTGCAGAGTATTTTGTGAGAAGATCAGAAAGGTTTTCCATGGACAACATGTTGTTCCAGAAGAAGGTGTTCGAAAAGTGTGGAGTTGGGGAAGAAACATACATACCCCCCCTTCATATTCAAAGACAACTACGAGGATATAACGTTGGAAGCTGCTTGGGAGGAGACAGGGGAGGTCGTTTATGGTGCTGTTGATGAGGTAATGCACAAGCTTAATATGGAAGCCGGTGATATAGGAATTATTGTGACAGTCTGTAGCGTTTTTAACCCCATTCCATCTCTGGCTTCTCACATAATGAATCATTACAAAATGAAGGAGGACACCAAATGCTTGCATCTGGCAGGAATGGGATGCAGCTCTGGAGTGATAGGAATCGATCTAGCAAGCAGTCTGCTCAAAACTTCCCGCGCAAACACAAATGCCCTTGTGGTAATCACTGAAAACATTTCTCTCAACTGGTATTTTGGAAACAACAAATCAATGCTGGTTACCAACTGTCTATTTCGTATGGGGTGCGCAGCTCTGATTCTGTCCAATAATCCCGGGGATAGAAACAGGGCAAAGCTGGAGCTGATTCATTCGCTGAGAACCCATACAGGCGCAGATGAAAAGGCCTACACTGCAGCATTTCAGGAGGAAGATGAAGAAGGCTATGTGGGAATCGGTCTCAAGAAAGAGTTGGTACCAGTGGCCAGTGAAGGCCTGGAGGCTCACATTACTGCTCTAGCTCCAATGGTGATGCCTTGTTCAGAGAAATTGCTCTACTTGCTGTATCTTGTGAAAAGGAAGGTCTTCAAAATGGATGTGAAGCCTTTTGTTCCCAACCTCACTCTGGCTTTTGAGCATTTCTGCATTCATACTGGAGGCAAGGCTGTCATTAACTCCATTGGCAAGCACCTTCGATTGAATGACTACTTGCTTGAGCCAGCGCGAATGGTTCTCCACAGATTTGGTAACACCTCCTGCAGTCTGGTGCTCTATGAGTTGGCTTACATGGAGGCCAAGAACAGGATTAAGAAAGGGGACAGGATATGGATGGTCTCCTTTGGGACTGGATTCAAGTGCAACACTCTTGTTTGGAAGGCCCTTCTTGATGTTGGAGACCCTGCTTACAACCCTTGGAATGACTGCATCCACAGATATCCAGTGAAATTATCATGAGATCTCATCCACATCACATCTCCATTCGCTATTATGGATTGATTCTATACTCTGCTGGATCAGTCCCACCCAAATCCTTTTGAGTATATATATATATATTATATCTGTATCTCTGTTGCCCATTGTTTAATCTTTGATGTGTTTTGGCCCTTGCTCGCCAAATAAAGATGCAGCACCCGTACAAATGCTGCCATTTGCTGATATTATGTTTATGCCTGATGTTTTTAACTATAACTCTCCAGTTATATACCACACAGTGGATCATCTGGAACATATTTGCAGCACTGCCCATATCAGCATGGATTGGCAGATTCTGCTCCCAACTTTTTCACCATTTCAATTAATTACGTTATTGATTGTTTTTATTTCCTTTTCCACACTTATAAATGGAATTTGGTTGATCTACTATGCACACTTTCTATGTGGAACCTTCAAGAA

Ace file of the UniGene sp_v2.0_unigene4181 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	3-ketoacyl-CoA synthase n=2 Tax=Selaginella moellendorffii RepID=D8REC4_SELML	-	-	50%
FL-Next	sp=3-ketoacyl-CoA synthase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	36%
Blast2go	3-ketoacyl- synthase 11-like	-	-	71%

Source	GOs	Term	Type	Identity
Blast2go	response to cold	GO:0009409	Biological Process	71%
Blast2go	very long-chain fatty acid metabolic process	GO:0000038	Biological Process	71%
Blast2go	wax biosynthetic process	GO:0010025	Biological Process	71%
Blast2go	cuticle development	GO:0042335	Biological Process	71%
Blast2go	fatty acid elongation	GO:0030497	Biological Process	71%
Blast2go	response to light stimulus	GO:0009416	Biological Process	71%
Blast2go	protein binding	GO:0005515	Molecular Function	71%
Blast2go	fatty acid elongase activity	GO:0009922	Molecular Function	71%
Blast2go	cytosolic ribosome	GO:0022626	Cellular Component	71%
Blast2go	membrane	GO:0016020	Cellular Component	71%
Blast2go	endoplasmic reticulum	GO:0005783	Cellular Component	71%

Source	InterPros	Term	Type	Identity
Sma3	ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain	IPR000194	-	-
Sma3	TonB box, conserved site	IPR010916	-	-
Sma3	Chalcone/stilbene synthase, C-terminal	IPR012328	-	-
Sma3	Very-long-chain 3-ketoacyl-CoA synthase	IPR012392	-	-
Sma3	FAE1/Type III polyketide synthase-like protein	IPR013601	-	-
Sma3	3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal	IPR013747	-	-
Sma3	Thiolase-like, subgroup	IPR016038	-	-
Sma3	Gonadotropin, beta subunit, conserved site	IPR018245	-	-
Sma3	Ribosomal protein S10, conserved site	IPR018268	-	-
Sma3	WD40 repeat, conserved site	IPR019775	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AD26

Fln msg: Separated hits, possible frame ERROR between 993 and 1028, your sequence is longer than subject: 519 - 486

Fln protein: MGSCKEWSEEKMTSRNALEGLMPAKLRQMVGKKFQAMKVESLLHTRLLTYLGYAVALILLFSYSWYVGNLITMDNLYALWKAGKVHKNSLIVLFWVVACLGVVWCMSRPRKIYMLDFACFKAAEDRKCTLELAEYFVRRSERFSMDNMLFQKKVFEKCGVGEETYIxxxxxxxxxxxxxTLEAAWEETGEVVYGAVDEVMHKLNMEAGDIGIIVTVCSVFNPIPSLASHIMNHYKMKEDTKCLHLAGMGCSSGVIGIDLASSLLKTSRANTNALVVITENISLNWYFGNNKSMLVTNCLFRMGCAALILSNNPGDRNRAKLELIHSLRTHTGADEKAYTAAFQEEDEEGYVGIGLKKELVPVASEGLEAHITALAPMVMPCSEKLLYLLYLVKRKVFKMDVKPFVPNLTLAFEHFCIHTGGKAVINSIGKHLRLNDYLLEPARMVLHRFGNTSCSLVLYELAYMEAKNRIKKGDRIWMVSFGTGFKCNTLVWKALLDVGDPAYNPWNDCIHRYPVKLS*

Protein Length: 520

Fln nts: CGTCGTGACCTTGGCTGTCATGGGAATCCTCTTTCTCTTACAGGGCTTGGATGATCAAAGCGTTCACTCGCCCAGGTGGAGAGTTTCTAACTGCTCTGCTCTGCTCTGTTCACTCTTATGTGGATCTGCAATTTGTCCCCGAGAGCAGATCGAGTATAGTTTCATTAGGTTCGTTTGTTGCAGTATATGAAGCGGTTTTCATCCCTACAAAGGTCTGTTTTTCCATAGAAAAAGTTGCATTTTCTTACATTTTTATGAAGTGATTTGTGAGCTCTAGGAAGCAGCTTTCTGCATTGACCAGGATTTGGTATTTAGTTTCTATTTGGCCAAGTTGTCGAATCGCAATCAAGGTTGAAAAGGCCTTTCGGACCATATATTTCATGCGTCTAGGGACCTTATTAATGAAGAAAGAAAACCATAGGTTATAGTTGTGATAAACTATAGATTAACCTAGTACCATTATTTGGCAGTCCGTTTATTTAATCTGAAAAA_-_ATGGGGTCCTGCAAAGAATGGAGTGAGGAAAAAATGACATCGCGAAATGCCCTTGAAGGTCTGATGCCTGCAAAACTAAGGCAAATGGTAGGAAAAAAATTTCAAGCCATGAAAGTGGAAAGTCTTCTTCACACTAGATTGCTCACATACTTGGGTTATGCGGTTGCACTCATTTTACTTTTCTCATATTCCTGGTACGTGGGGAATCTGATCACAATGGACAACTTGTATGCTCTGTGGAAGGCCGGCAAGGTGCACAAAAATAGTCTTATAGTGTTATTTTGGGTGGTGGCATGCCTGGGTGTTGTTTGGTGTATGTCTAGGCCAAGAAAAATCTACATGCTGGATTTCGCCTGCTTCAAAGCAGCGGAAGATCGAAAATGCACCCTTGAACTTGCAGAGTATTTTGTGAGAAGATCAGAAAGGTTTTCCATGGACAACATGTTGTTCCAGAAGAAGGTGTTCGAAAAGTGTGGAGTTGGGGAAGAAACATACATACCxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxAACGTTGGAAGCTGCTTGGGAGGAGACAGGGGAGGTCGTTTATGGTGCTGTTGATGAGGTAATGCACAAGCTTAATATGGAAGCCGGTGATATAGGAATTATTGTGACAGTCTGTAGCGTTTTTAACCCCATTCCATCTCTGGCTTCTCACATAATGAATCATTACAAAATGAAGGAGGACACCAAATGCTTGCATCTGGCAGGAATGGGATGCAGCTCTGGAGTGATAGGAATCGATCTAGCAAGCAGTCTGCTCAAAACTTCCCGCGCAAACACAAATGCCCTTGTGGTAATCACTGAAAACATTTCTCTCAACTGGTATTTTGGAAACAACAAATCAATGCTGGTTACCAACTGTCTATTTCGTATGGGGTGCGCAGCTCTGATTCTGTCCAATAATCCCGGGGATAGAAACAGGGCAAAGCTGGAGCTGATTCATTCGCTGAGAACCCATACAGGCGCAGATGAAAAGGCCTACACTGCAGCATTTCAGGAGGAAGATGAAGAAGGCTATGTGGGAATCGGTCTCAAGAAAGAGTTGGTACCAGTGGCCAGTGAAGGCCTGGAGGCTCACATTACTGCTCTAGCTCCAATGGTGATGCCTTGTTCAGAGAAATTGCTCTACTTGCTGTATCTTGTGAAAAGGAAGGTCTTCAAAATGGATGTGAAGCCTTTTGTTCCCAACCTCACTCTGGCTTTTGAGCATTTCTGCATTCATACTGGAGGCAAGGCTGTCATTAACTCCATTGGCAAGCACCTTCGATTGAATGACTACTTGCTTGAGCCAGCGCGAATGGTTCTCCACAGATTTGGTAACACCTCCTGCAGTCTGGTGCTCTATGAGTTGGCTTACATGGAGGCCAAGAACAGGATTAAGAAAGGGGACAGGATATGGATGGTCTCCTTTGGGACTGGATTCAAGTGCAACACTCTTGTTTGGAAGGCCCTTCTTGATGTTGGAGACCCTGCTTACAACCCTTGGAATGACTGCATCCACAGATATCCAGTGAAATTATCATGA___GATCTCATCCACATCACATCTCCATTCGCTATTATGGATTGATTCTATACTCTGCTGGATCAGTCCCACCCAAATCCTTTTGAGTATATATATATATATTATATCTGTATCTCTGTTGCCCATTGTTTAATCTTTGATGTGTTTTGGCCCTTGCTCGCCAAATAAAGATGCAGCACCCGTACAAATGCTGCCATTTGCTGATATTATGTTTATGCCTGATGTTTTTAACTATAACTCTCCAGTTATATACCACACAGTGGATCATCTGGAACATATTTGCAGCACTGCCCATATCAGCATGGATTGGCAGATTCTGCTCCCAACTTTTTCACCATTTCAATTAATTACGTTATTGATTGTTTTTATTTCCTTTTCCACACTTATAAATGGAATTTGGTTGATCTACTATGCACACTTTCTATGTGGAACCTTCAAGAA

Fln Alignment:

Contig4181___IVLFWVVACLGVVWCMSRPRKIYMLDFACFKAAEDRKCTLELAEYFVRRSE---RFSMDNMLFQKKVFEKCGVGEETYIPxxxxxxxxxxxxTLEAAWEETGEVVYGAVDEVMHKLNMEAGDIGIIVTVCSVFNPIPSLASHIMNHYKMKEDTKCLHLAGMGCSSGVIGIDLASSLLKTSRANTNALVVITENISLNWYFGNNKSMLVTNCLFRMGCAALILSNNPGDRNRAKLELIHSLRTHTGADEKAYTAAFQEEDEEGYVGIGLKKELVPVASEGLEAHITALAPMVMPCSEKXXXXXXLVKRKVFKM-DVKPFVPNLTLAFEHFCIHTGGKAVINSIGKHLRLNDYLLEPARMVLHRFGNTSCSLVLYELAYMEAKNRIKKGDRIWMVSFGTGFKCNTLVWKALLDVGDPAYNPWNDCIHRYPVKLS
D5AD26______________ILALLMILTLLFMYYMSRPQVVYLVDFACFKPSYESKVS---REFFLRMSKDTGKFEEPSLAFQRKIVEKSGLGDETHLPxxxxxxxxxxxxSLNEARAEAEAVMFGALDELFMKSKVRAEDIGILVVNCSLFNPTPSLSSMIINYYKMRGNILSFNLGGMGCSAGIIAVDLARDLLQV-HSNTYAIVLSTENITLNWYTGNDRSMLLPNCLFRMGGSAILLSNRRTERRRSRYQLKHIVRTHKGAEDESYQCVYQGEDDNNEKGLSISRDLMSVAGHALKANITTLGPLVLPLSEQLLFLWALFCRKVLKMRDVRPYIPNFKLAFDHFFIHAGGRGVLDGVEKNLGLTKYHMEPSRMTLHRFGNTSSSSLWYELAYSEAKGRVKKGDRVWQIAFGSGFKCNSAVWKALRDVEVPLHNPWLDCIHNYPVELT

SNPs (tot: 6)

Position	A %	C %	G %	T %	Probability
2336		14		85	0.993533
2396	12	87			0.992554
2465		80		20	0.995493
2467		75	25		0.996473
2468	25	75			0.996473
2469	75		25		0.996473

SSRs (tot: 2)

Start position	End position	Sequence	Length
340	354	AT AT AT AT AT AT AT A	15
2378	2395	CAGAG CAGAG CAGAG CAG	18

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain