SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene4020

Length: 1260 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene4020 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	putative ATP-dependent RNA helicase [Oryza sativa]	-	-	50%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	29%
Blast2go	dead-box atp-dependent rna helicase 17-like	-	-	66%

Source	GOs	Term	Type	e value	Identity
Blast2go	helicase activity	GO:0004386	Molecular Function	0.0	66%
Blast2go	binding	GO:0005488	Molecular Function	0.0	66%

Source	InterPros	Term	Type	Identity
Sma3	RNA helicase, ATP-dependent, DEAD-box, conserved site	IPR000629	-	-
Sma3	Helicase, C-terminal	IPR001650	-	-
Sma3	DNA/RNA helicase, DEAD/DEAH box type, N-terminal	IPR011545	-	-
Sma3	Helicase, superfamily 1/2, ATP-binding domain	IPR014001	-	-
Sma3	RNA helicase, DEAD-box type, Q motif	IPR014014	-	-
Sma3		IPR014021	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: B8LKW8

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 113 aas, your sequence is shorter than subject: 331 - 802

Fln protein: FDDEYQRQNLLLSATLNEEVNDLAKISLNNPVTVGLKERIFSQGKTKLYSGLSAEALTNEWALPGTCSSASEYRFPAKLVQSYIKVPCRLRFVVLLALLRSQFEAEPSRKVVVFFSTCDAVDFHYAAISEFHWVSHFQEVDEEKGRFIKCKTFRLHGNMAQAERKETFHGYNEEKCALLLCTDVAARGLDFPKVGCIIQYDSPGDANEYVHRVGRTARLDQKGEAVLFLQPIELDYLKELQKHGVPLNELSVQNVLDHIQVRGRTYNKQAKWSAVDMHPGALAMQKALESFIMMEESMKQLAKDAFCSFVRAYAAHRGDLKRYFRLSSFT*

Protein Length: 332

Fln nts: TTTGATGATGAGTACCAGAGGCAGAACTTGCTCTTGTCAGCAACACTGAATGAGGAAGTTAATGATCTTGCAAAAATAAGCTTGAACAATCCTGTTACGGTTGGGCTGAAAGAGAGAATTTTTTCTCAAGGTAAGACCAAATTATATTCTGGTCTTTCTGCAGAAGCACTAACAAATGAATGGGCTCTGCCTGGAACTTGTAGTTCAGCATCAGAATACAGATTTCCAGCTAAGTTAGTTCAGAGTTACATCAAGGTGCCATGTCGTTTAAGGTTTGTAGTGTTGTTGGCTCTTTTGCGATCTCAATTTGAAGCAGAACCTTCTCGCAAGGTTGTTGTTTTCTTCTCAACATGTGATGCAGTGGATTTCCACTATGCTGCCATAAGTGAGTTTCACTGGGTTTCACATTTTCAAGAGGTGGATGAAGAAAAGGGAAGATTCATAAAATGCAAAACATTTCGTCTACATGGAAATATGGCACAAGCAGAACGAAAGGAAACTTTTCATGGATATAATGAAGAAAAATGTGCTCTTCTCTTGTGCACTGATGTAGCAGCAAGAGGCTTGGACTTTCCGAAAGTTGGATGCATTATTCAGTATGACTCTCCAGGAGATGCAAATGAATATGTGCACAGGGTTGGTAGAACAGCTCGTCTAGACCAGAAAGGTGAAGCTGTGTTATTTTTGCAGCCTATTGAGTTGGATTATTTGAAAGAATTGCAGAAGCATGGCGTGCCCTTGAATGAGCTTTCAGTTCAAAATGTTTTGGATCATATTCAAGTGCGTGGAAGGACATATAATAAGCAAGCAAAATGGTCTGCAGTAGATATGCATCCCGGGGCATTGGCTATGCAGAAGGCTTTGGAATCATTCATTATGATGGAGGAAAGTATGAAACAGCTTGCAAAAGATGCATTCTGTTCTTTTGTACGTGCATATGCAGCCCACCGAGGTGATCTTAAAAGATATTTCAGGTTAAGCAGCTTCACTTAGGGCATGTTGCAAAGAGCTTTGGGCTAAAAGACCAGCCTTCGTTGGTTGGGAAATCATTTAACAAGCAAGCCAAGAAAAGGGAGAGAAGTCAAAAGTTTAGGAAAGGTCCCAAAAAAATGCGACTTACACGAGTCACGTCAGTTGACAAATGAAGTGTTCTTTTGTATTATCTATATTTGGTACATAGAAGCTTCAGTATTGGGGTCCTCATTTTCAGCATGATGTGCTCAATTGATGGTTGTATGAGGCATGTCAGCAAAAGTTTTG

Fln Alignment:

Contig4020___QRQNLLLSATLNEEVNDLAKISLNNPVTVGLKERIFSQGKTKLYSGLSAEALTNEWALPGTCSSASEYRFPAKLVQSYIKVPCXXXXXXXXXXXRSQFEAEPSRKVVVFFSTCDAVDFHYAAISEFHWVSHFQEVDEEKGRFIKCKTFRLHGNMAQAERKETFHGYNEEKCALLLCTDVAARGLDFPKVGCIIQYDSPGDANEYVHRVGRTARLDQKGEAVL
B8LKW8______________ERQSMLFSATMPGWVKKLSRKHLNNPMTIDL----VGENEEKLAEGIKL------YAVATTTSSNSKRSILGDLITVFAK----------------------GGKTIVFTQTKRDAD----------------EISLGLSSTIACEA--LHGDISQHQRERTLAGFREGKFSALVATDVAARGLDITNVDLVIHYEFPNDPETFVHRSGRTGRAGKEGIAIL

SNPs (tot: 6)

Position	A %	C %	G %	T %	Probability
868	33		66		0.997452
871	66			33	0.997452
885	33	66			0.997452
919	33		66		0.997452
1042	14			85	0.993533
1048		14		85	0.993533

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain