SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene3946

Length: 524 nt

Origin of reads: Biogeco2 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene3946
CCAAAATATGTCCTTGGTTCAAAGGAAAACAGTAAACATGTGATCGCCACAAACATGCAAATAACCATCAAGTTATACAGTGATAGACGCATGAATAATCTGTAGCATGCTAGCATTTAGTAAGTTGTAAGAAGGTTTTGGAAACTACTATCAATACAAGAAATACATATCTAGAAAAGACATATACCGGACATGCAGCCAATTCCATATGCCAGATCTGTTGTCCTGCATGCTCTCTGACGTTGACAGGCAAGGTGAAGGTGAATCTTTGATGGACAACGATATTAGTGACAGCTCTGTTCTGCTTTTATGTGCAGGTCACAAAACTACCAGCTCAACTTTGACAACACTGCGGATATTTCTGGCAGAGTAGACTCGCTGAGTAGCTGCTAGGAAGAAATATGATATGGTAATTCCATATTGACGAGTCTCCGAATCTGCCTGTACATATTAAATCAATCATTATCACTTTGTTTTCAATTACTAGCCTTCTGCCCATTGTCAATAACTCTTTTTGAATATAC

Ace file of the UniGene sp_v2.0_unigene3946 (antisense)

If you push the Download ace button, you will download an ace file of this UniGene.

To watch this ACE file you will need an ACE viewer program as Tablet:

Go to the Tablet ACE viewer Web

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Cytochrome P450 716B2 n=2 Tax=Picea sitchensis RepID=C16B2_PICSI	-	-	8.0e-08	65%
FL-Next	sp=Cytochrome P450 716B1; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	65%
Blast2go	c16b1_picsi ame: full=cytochrome p450 716b1 ame: full=cytochrome p450 cypa1	-	-	2.44593e-08	72%

Source	GOs	Term	Type	e value	Identity
Blast2go	oxidoreductase activity	GO:0016491	Molecular Function	2.44593e-08	72%
Blast2go	iron ion binding	GO:0005506	Molecular Function	2.44593e-08	72%
Blast2go	membrane	GO:0016020	Cellular Component	2.44593e-08	72%

Source	InterPros	Term	Type	Identity
Sma3	Cytochrome P450	IPR001128	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3		IPR017973	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: Q50EK1

Fln msg: Unexpected stop codon in the beginning of your sequence, Unexpected STOP codon at 3' end. Distance to subject end: 120 aas, your sequence is shorter than subject: 124 - 493

Fln protein: QNMSLVQRKTVNM*SPQTCK*PSSYTVIDA*IICSMLAFSKL*EGFGNYYQYKKYISRKDIYRTCSQFHMPDLLSCMLSDVDRQGEGESLMDNDISDSSVLLLCAGHKTTSSTLTTLRIFLAE*

Protein Length: 125

Fln nts: CCAAAATATGTCCTTGGTTCAAAGGAAAACAGTAAACATGTGATCGCCACAAACATGCAAATAACCATCAAGTTATACAGTGATAGACGCATGAATAATCTGTAGCATGCTAGCATTTAGTAAGTTGTAAGAAGGTTTTGGAAACTACTATCAATACAAGAAATACATATCTAGAAAAGACATATACCGGACATGCAGCCAATTCCATATGCCAGATCTGTTGTCCTGCATGCTCTCTGACGTTGACAGGCAAGGTGAAGGTGAATCTTTGATGGACAACGATATTAGTGACAGCTCTGTTCTGCTTTTATGTGCAGGTCACAAAACTACCAGCTCAACTTTGACAACACTGCGGATATTTCTGGCAGAGTAGACTCGCTGAGTAGCTGCTAGGAAGAAATATGATATGGTAATTCCATATTGACGAGTCTCCGAATCTGCCTGTACATATTAAATCAATCATTATCACTTTGTTTTCAATTACTAGCCTTCTGCCCATTGTCAATAACTCTTTTTGAATATAC

Fln Alignment:

Contig3946___DLLSCMLSDVDRQGEGESLMDNDISDSSVLLLCAGHKTTSSTLTTLRIFLAE
Q50EK1______________DLLSFLLSNVDEQGE--SLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAE

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
93	33		66		0.997452
110	75		25		0.996473
139		50		50	1.0
211		83		16	0.994512
219	55	22	11	11	0.999999
220	11		88		0.923564
222	11	88			0.923564
223			22	11	0.999999
224	66	11		22	0.999999
227	66	33			1.0
229	66			33	1.0
230			66	33	1.0
231	66		33		1.0
238	88		11		0.923564
265	88		11		0.923564
266		88		11	0.923564
268			11	88	0.923564
270		87	12		0.992554
277		88	11		0.923564
376	66		33		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain