SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene3899

Length: 1958 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uma v2

UniGene Fasta

>sp_v2.0_unigene3899
GAAGGGATCCATTTGTATTCTAGGTCCTCTTCCCTATTTCTAACTAATTCCAAAATTTGACTGCATATTCATTCATTTGAACTGCTGGTTATTGATTCGTTTTCTTGGTCTTCTCCACTCCAAATCAATTTTCAATGCGTTGCAGGTGCCAAGTTCTGCGAACGGATGGCATTTTATGGAGTGGACACAAATTTAGTGACTTATTTGACTGGAGAACTGAATAAGAGCAACGCTGTGGCAGCAACTATTTCAACAACCTGGTTAGGCACATGCAATGTCACACCACTTCTGGGAGCAATGTCAGATGCATACCTGGGGCGCTACAAGACCATGGTTATTGCATCCACGCTCTATTTCACTGGATTAATCACACTGACATTGGTGAGTGTCTTGAAGCTCCCAGCTATTGTATTCTACGTGGGACTTTATGTGATGGCTTTGGGAAGTGGAAGCTTCATGCCATGCATTGTGGCTTTTGGAGCAGATCAATTCAATGACACCAATACAAAAGAAAGGATGATAAAGAGTTCATTTTTCAATTGGTTCTTCTTTGTCAGTAATTTTGGGACCCTGCTTTCTAGTACTGGTGTGGTATGGGTGGAAGACAATGTAAGCTGGGCACTGGGCTTTGGGATTTCAACTCTAATCATGGGATTTGCTATGGGGTCTTTCTACTCTGGTACACCTATTTACAGACACCAAAAACCAGGTGGCAGTCCCTTCACACGAATATGTCAGGTGATTGTTGCCTCTTTGCATAAACTACGAGTGCAGGTCCCCTGTGATAAGAGCCTCCTTTATGAAAAGGAACACAAGGAGGAATCCGCTGTTCCTGGAAGTCGAAAACTAAATTACACAGATGAATTCAAGTGTTTAGATAAGGCAGCAACAGTAAGCGGTGATGAGAGCAACAGTGGTGAGTTGTGCTTGAATGGTTGGAGGATTTGTACAGTAACCCAAGTGGAAGAAGTGAAAATATTGCTTCGAATGGTACCCATATGGGCAACTGCTATAATATATACATCAGCATACGCCCAGGCACACACCATGTTCGTGGAACAGGGCAAGACAATGAACAATAGCATAGGTGGCCATTTCAATATACCTGCGGCTTCACTGTACATGTTCAACAGTGTAACCTGCATGCTCTGGGTTCTACTATATGATTTTAGCATCGCACCAGCAATGAGAAAATTCAAGGGAAACGAGAAGGGGTTCTCCGAGTTGCAACGAATGGGTATAGGTAAGTTATTCGCCATGTTTTCTATGGTGTGTGCTGCACTTCTTGAAATCAAACGATTGCAAGTTGTGAGGAGTCACGACCTGGTTCATGATTCAGAGACACCAGTTCCTCTAAGCATTTTGTGGCAGATACCACAGTATGTATTGTTAGGCGTTGCAGAGACATTTGTATTGGCAGGGCAACTGGAGTTCTTCTACGAAGAGGCCCCTGATGCCATGCGAACCCTGTGCAGTGCTCTGTCACATCTTAGTACGGCATTCGGTAACTATTTTAGTTCGGTATTAGTGATTGTTGTAATGCTTATTACCACCAGAGGCGGGAGTGTAGGATGGATACCCAATAACTTGAATGAAGGTCACCTGGACTACTACTTTTGGCTGTGGGCTGCACTGAGTTTCCTCAATTTGGCTCTATATGTTGCTGCTGCGCGTAAATACAGAAGTAAATATGTGGCCCATGAGATGAGGGATGTTTTCATTTGAATACAATCCTCATAGTCAATGTATAATAACATGCGTCTTGCCAAGTTTCTTGACCAGTTATAAATGTTGTAAGAAATGTATAATAACCTGTGTCTTCCCAAGTTTCTTGACCAGTTATAAATGTTGGAAGAAATGTTCACCAGTTATAAATAAATAGCCCCATGGCTCCAAGGGTGTTGTCAGAACGCTTCTCGGATAGAATTTGTCTGAATAATTTCTTAGTTTAATTCTTA

Ace file of the UniGene sp_v2.0_unigene3899

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	peptide transporter [Hakea actites]	-	-	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	57%
Blast2go	peptide transporter ptr2-like	-	-	70%

Source	GOs	Term	Type	e value	Identity
Blast2go	transport	GO:0006810	Biological Process	0.0	70%
Blast2go	membrane	GO:0016020	Cellular Component	0.0	70%

Source	InterPros	Term	Type	Identity
Sma3	Oligopeptide transporter	IPR000109	-	-
Sma3	2-oxo acid dehydrogenase, lipoyl-binding site	IPR003016	-	-
Sma3	Aldehyde dehydrogenase, conserved site	IPR016160	-	-
Sma3	PTR2 family proton/oligopeptide symporter, conserved site	IPR018456	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NVR2

Fln msg: atg_distance in limit (1-15): atg_distance = 13, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 520 - 594

Fln protein: MAFYGVDTNLVTYLTGELNKSNAVAATISTTWLGTCNVTPLLGAMSDAYLGRYKTMVIASTLYFTGLITLTLVSVLKLPAIVFYVGLYVMALGSGSFMPCIVAFGADQFNDTNTKERMIKSSFFNWFFFVSNFGTLLSSTGVVWVEDNVSWALGFGISTLIMGFAMGSFYSGTPIYRHQKPGGSPFTRICQVIVASLHKLRVQVPCDKSLLYEKEHKEESAVPGSRKLNYTDEFKCLDKAATVSGDESNSGELCLNGWRICTVTQVEEVKILLRMVPIWATAIIYTSAYAQAHTMFVEQGKTMNNSIGGHFNIPAASLYMFNSVTCMLWVLLYDFSIAPAMRKFKGNEKGFSELQRMGIGKLFAMFSMVCAALLEIKRLQVVRSHDLVHDSETPVPLSILWQIPQYVLLGVAETFVLAGQLEFFYEEAPDAMRTLCSALSHLSTAFGNYFSSVLVIVVMLITTRGGSVGWIPNNLNEGHLDYYFWLWAALSFLNLALYVAAARKYRSKYVAHEMRDVFI*

Protein Length: 521

Fln nts: GAAGGGATCCATTTGTATTCTAGGTCCTCTTCCCTATTTCTAACTAATTCCAAAATTTGACTGCATATTCATTCATTTGAACTGCTGGTTATTGATTCGTTTTCTTGGTCTTCTCCACTCCAAATCAATTTTCAATGCGTTGCAGGTGCCAAGTTCTGCGAACGG_-_ATGGCATTTTATGGAGTGGACACAAATTTAGTGACTTATTTGACTGGAGAACTGAATAAGAGCAACGCTGTGGCAGCAACTATTTCAACAACCTGGTTAGGCACATGCAATGTCACACCACTTCTGGGAGCAATGTCAGATGCATACCTGGGGCGCTACAAGACCATGGTTATTGCATCCACGCTCTATTTCACTGGATTAATCACACTGACATTGGTGAGTGTCTTGAAGCTCCCAGCTATTGTATTCTACGTGGGACTTTATGTGATGGCTTTGGGAAGTGGAAGCTTCATGCCATGCATTGTGGCTTTTGGAGCAGATCAATTCAATGACACCAATACAAAAGAAAGGATGATAAAGAGTTCATTTTTCAATTGGTTCTTCTTTGTCAGTAATTTTGGGACCCTGCTTTCTAGTACTGGTGTGGTATGGGTGGAAGACAATGTAAGCTGGGCACTGGGCTTTGGGATTTCAACTCTAATCATGGGATTTGCTATGGGGTCTTTCTACTCTGGTACACCTATTTACAGACACCAAAAACCAGGTGGCAGTCCCTTCACACGAATATGTCAGGTGATTGTTGCCTCTTTGCATAAACTACGAGTGCAGGTCCCCTGTGATAAGAGCCTCCTTTATGAAAAGGAACACAAGGAGGAATCCGCTGTTCCTGGAAGTCGAAAACTAAATTACACAGATGAATTCAAGTGTTTAGATAAGGCAGCAACAGTAAGCGGTGATGAGAGCAACAGTGGTGAGTTGTGCTTGAATGGTTGGAGGATTTGTACAGTAACCCAAGTGGAAGAAGTGAAAATATTGCTTCGAATGGTACCCATATGGGCAACTGCTATAATATATACATCAGCATACGCCCAGGCACACACCATGTTCGTGGAACAGGGCAAGACAATGAACAATAGCATAGGTGGCCATTTCAATATACCTGCGGCTTCACTGTACATGTTCAACAGTGTAACCTGCATGCTCTGGGTTCTACTATATGATTTTAGCATCGCACCAGCAATGAGAAAATTCAAGGGAAACGAGAAGGGGTTCTCCGAGTTGCAACGAATGGGTATAGGTAAGTTATTCGCCATGTTTTCTATGGTGTGTGCTGCACTTCTTGAAATCAAACGATTGCAAGTTGTGAGGAGTCACGACCTGGTTCATGATTCAGAGACACCAGTTCCTCTAAGCATTTTGTGGCAGATACCACAGTATGTATTGTTAGGCGTTGCAGAGACATTTGTATTGGCAGGGCAACTGGAGTTCTTCTACGAAGAGGCCCCTGATGCCATGCGAACCCTGTGCAGTGCTCTGTCACATCTTAGTACGGCATTCGGTAACTATTTTAGTTCGGTATTAGTGATTGTTGTAATGCTTATTACCACCAGAGGCGGGAGTGTAGGATGGATACCCAATAACTTGAATGAAGGTCACCTGGACTACTACTTTTGGCTGTGGGCTGCACTGAGTTTCCTCAATTTGGCTCTATATGTTGCTGCTGCGCGTAAATACAGAAGTAAATATGTGGCCCATGAGATGAGGGATGTTTTCATTTGAATACAATCCTCATAGTCAATGTATAATAACATGCGTCTTGCCAAGTTTCTTGACCAGTTATAAATGTTGTAAGAAATGTATAATAACCTGTGTCTTCCCAAGTTTCTTGACCAGTTATAAATGTTGGAAGAAATGTTCACCAGTTATAAATAAATAGCCCCATGGCTCCAAGGGTGTTGTCAGAACGCTTCTCGGATAGAATTTGTCTGAATAATTTCTTAGTTTAATTCTTA

Fln Alignment:

Contig3899___VAGAKFCERMAFYGVDTNLVTYLTGELNKSNAVAATISTTWLGTCNVTPLLGA-MSDAYLGRYKTMVIASTLYFTGLITLTL---VSVLKLPAI--------------VFYVGLYVMALGSGSFMPCIVAFGADQFNDTNTKERMIKXXXXXXXXXXXXXGTLLSSTGVVWVEDNVSWALGFGISTLIMGFAMGSFYSGTPIYRHQKPGGSPFTRICQVIVASLHKLRVQVPCDKSLLYEKEHKEESAVPGSRKLNYTDEFKCLDKAATVSGDESNSGELCLNGWRICTVTQVEEVKILLRMVPIWATAIIYTSAYAQAHTMFVEQGKTMNNSIGGHFNIPAASLYMFNSVTCMLWVLLYDFSIAPAMRKFKGNEKGFSELQRMGIGKLFAMFSMVCAALLEIKRLQVVRSHDLVHDSETPVPLSILWQIPQYVLLGVAETFVLAGQLEFFYEEAPDAMRTLCSALSHLSTAFGNYFSSVLVIVVMLITTRGGSVGWIPNNLNEGHLDYYFWLWAALSFLNLALYVAAARKYRSK
A9NVR2______________ILGNECCERLAYYGINTNLVTYLTKRLHQGNAAAAKSVTTWAGTCYLTPLFGAVLADAYWGRYWTIAAFSTIYFIGMATLTLSASVSSLKPPSCIGSDCPTANLAQYGVFFLGLYLIALGTGGIKPCVSSFGADQFDDTDPKEKKKKGSFFNWFYFSINVGALVSSSVLVWVQDNVGWGWGFGIPTLFMGLAIGSFFSGTPLYRLQKPGGSPVTRMCQVVVASLRKLRKTVPLDHSHLYEVQDRN-SAIQGSRKLEHTDEFKCLDKSAIITDDDVRKGGFS-NPWRLCTVTQVEEMKILLRMFPIWATGIVFAAVYSQISTMFVEQGMTLDTSIGSRFHIPPASLSVFDVVSVMIWVPVYDRVIVPIVSKYTKRERGFTELQRMGIGLFISILAMVAAALVEIRRLNIVKTYDLVYDKGTPVPMSIFWQIPQYFLVGASEVFTFVGQLEFFYDQSPDAMRSLCSALSLLTTALGNYLSSLIVTIVMFATTKGGNIGWIPDNLNEGHLDYYFWVLASLSVLNLLVYVSCAHRYKYK

SNPs (tot: 21)

Position	A %	C %	G %	T %	Probability
744	20		80		0.995493
869	87		12		0.992554
891	50		50		1.0
918		75	25		1.0
920	25		75		1.0
982	87	12			0.992554
999	71		28		1.0
1012	85		14		0.993533
1015		14	85		0.993533
1016	14	85			0.993533
1017			14	85	0.993533
1075	80		20		0.995493
1092	85			14	0.993533
1137	20	80			0.995493
1139		20	80		0.995493
1603	25		75		0.996473
1604		25	75		0.996473
1606		75		25	0.996473
1639		66		33	0.997452
1759	33	66			1.0
1808	25		75		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain