SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene3756

Length: 1277 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene3756

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Drf_FH1 multi-domain protein	-	-	2.0e-07	38%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	83%
Blast2go	unknown [Picea sitchensis]	-	-	6.30577e-32	89%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5A8Y8

Fln msg: Overlapping hits, possible frame ERROR between 294 and 276, W1: There is no M at the beginning, your sequence is longer than subject: 124 - 90

Fln protein: WECHDLESHNSQCHLVLTSLFNLQEWDSTANHPCQQLHQFLGNKILKxxxxxxxxYSNP*KLLEKVGMNFLIALVSICIRLCNCSCTAFVFYSWRLVQICTKFLRSHFAHIIATIYFMHGLKY*

Protein Length: 125

Fln nts: CGTCGTGACCTTGGCTGTCACCACCTCCACCACCAGTACAGTATATGCCCTGTAATGGGAATATGCTGAGCATGCCCTACGGGTATGGTAGTGGTTTGCCACCACCTCCCCCCTTACCTGGACATCCAATA_-_ATGGGAGTGCCACGACCTGGAATCTCACAACAGTCAGTGCCACCTGGTCCTTACCAGCCTTTTCAACCTCCAGGAATGGGATTCTACAGCCAACCACCCTTGCCAACAGCTCCACCAGTTCCTCGGCAATAAAATTCTCAAGxxxxxxxxxxxxxxxxxxxxxxxxTATAGCAACCCTTGAAAGTTACTTGAGAAAGTTGGCATGAATTTCCTTATTGCTTTGGTGAGCATCTGTATTAGATTATGTAATTGTTCATGCACAGCATTTGTTTTTTATTCCTGGCGGCTAGTCCAGATCTGTACAAAATTTTTGAGGTCCCATTTTGCACATATCATTGCAACAATCTATTTTATGCACGGCCTAAAATACTAATTGCTGCATATTGTGCATGCAATTGATACATAATTATTCACCCCCTATTTCCAATGAAGCAAAAAAGCAAACGAGAGGAAAATTGAAGGGGAGTTGCACGCAAATTGCGATAGATGGAAGTATGAGTAGTGTTTCATGTTTTTGAGTTACTAAAGCAATCATCCTAAAATTTCAAGGAGGGAAGAAATGTGATTCTGGAGGGGATTCCATGGCACGTTGTAATTTATCATTTTAGTACGATTTAATTTTTACATGCAGAGATCCCATGCCTGCTCCTCTTGACCTGGGAACTTGTGCTCCAAGGTTTAATTTGTACTTTCAAATGAACAACAGGTCACTACAGAGAATGTGCAACTTGCAAAGCGTACATTACCTTCGTTCCCATTTGTTTACAAGTTTCATTCGAGTATTATACAGCTGACAATGTTTTCCAGCAAAAACAGTGTTAAGATAACATTGGTTCTAAGACCTCACAAATCCCAAATTCTACTTAAAACTCCAGATTTCTTGTTATAAGAGAACAGATTTGAACTTTACCAAGGGAACCGGAAGTTTTCCTTTGGTATCACTACTATTTCTTGTTCATCTCAGGGATGTGAATTCATATCTATTTTGAATCCCGGTTTCAGTGACGTCAAGCATAGAATGTGAATTACAGCTTCACAACTTGAATATTTCTTACATGTAATTTTATGACGACAGATTTACTTCACATTTGAGGTTCCCATTTTGGAACAATAGTTCGCAAATATCTAAGTTCAATGTAGTTATTTA

Fln Alignment:

Contig3756___LESHNSQCHLVLTSLFNLQEWDSTANHPCQQLHQFLGNKILKxxxxxxxYSNP*KLLEKVGMNFLIALVSICIRLCNCSCTAFVFY
D5A8Y8______________MESLNSRCHLVLTSLFNLQGWDSTANHPCQQFHQFLGNKILKxxxxxxxCSNPRKLLEKVGMSFLIALVSICIRLCNCSCTAFVFF

SNPs (tot: 39)

Position	A %	C %	G %	T %	Probability
484			14	85	0.993533
485			14	85	0.993533
629	50		50		1.0
647		14		85	0.993533
648	14			85	0.993533
649		14		85	0.993533
651		14	85		0.993533
762	80		20		0.995493
764		20	80		0.995493
765		80	20		0.995493
766	80			20	0.995493
769		20	80		0.995493
770	80	20			0.995493
772		80		20	0.995493
773		80	20		0.995493
774		80	20		0.995493
775	80	20			0.995493
781		20	80		0.995493
782	20	80			0.995493
785		83	16		0.994512
788			16	83	0.994512
795			83	16	0.994512
807	16			83	0.994512
809		83		16	0.994512
810	83	16			0.994512
966	16			83	0.994512
967		16		83	0.994512
968	16		83		0.994512
969			83	16	0.994512
993	83	16			0.994512
994			16	83	0.994512
1002	83		16		0.994512
1003	83		16		0.994512
1004	83	16			0.994512
1005	83			16	0.994512
1006		83	16		0.994512
1010	83			16	0.994512
1011		16	83		0.994512
1025	85		14		0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain