SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene3713

Length: 2219 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene3713
CTTTGGCCATAGTAAATAGCGCTGGTGGGTAACAAGCGCTATTTACTATGGCCAAAGGGTCTTACGGAGCCTTTCATTGTTTCTTCTGTTGAAGCATGTGATTCAACAGAAGAAACAATGTAAGGGTCTTAGGAGCCTTTCATTGTTTCTTCTGTTTGAATCACATGCTTCAAATTTTGAGGATGAGGATGAAGATGTAACGGCAGAGAGGGTGAGAGTTCTATCTGGTGCAGCGAAAGAGAGCATAATTTACCTACAGAATCTCAGAAAGATATATCCATCAAGTGGAAATCAGCCAGAGAAGGTTGCAGTTGATTCCTTGAGCTTTGCAGTTCAAGAAGGAGAGTGTTTTGGATTTCTCGGCACAAATGGTGCTGGAAAGAGTACAACACTGTCAATGCTATGTGGCGAAGAGCATCCTACGGGAGGAACTGCATATATTTTTGGCAAGGATATTCTTGCACATCCAAAGGCAGCACATCAGCATGTAGGTTATTGTCCTCAGTTTGATGCCCTTCTAGAGTTACTTACTGTTCGAGAACATCTCGAGCTCTATGCACGGATCAAGGGAGTTTCAAAGGAAATATTAGATCAAGTTGTCGAAGGAAAGCTCGCAGAATTTGATTTATGGCCACAAGCAAACAAACCTTCCTGTTCTCTAAGTGGTGGCAATAAACGCAAGTTATCAGTGGCCATAGCAATGATTGGAGATCCTCCTCTCGTCATTTTAGATGAACCTTCAACGGGTATGGACCCTATTGCAAAGCGATTTATGTGGGATGTCATCTCACGTATATCAACTAGGCAAGGGAAAACAGCAGTTATCCTGACAACACATAGCATGAATGAAGCACAGGCCCTTTGTACACGCATTGGAATTATGGTTGGGGGGCGGCTCTGTTGTCTTGGAAAGTCCTCAACATTTGAAAACACGCTTTGGCAACTATCTTGAACTTGAGGTGAAACCTGTGGATGTTAGTGTGGTGGAATTAGATAACTTGTGCAATACTCTACATGATATGTTTTTTGACTTTCCGAGCCATTCTAGAGGTATCTTGAGTGATCTGGAAATTTGTATTGGAGGATCAGAGGCTGGCTCCTCCACTCACATGTCAGAAACTAATTTGTCACATGAAATGATCATTGTTGCGGGGCGTAGACTAGGCAATGAGGAGTATGTGCAAAACCTAATTTCAAACAGCCCTTGCATCGATGGAGTTTTTAGTGAGCAATTATCAGAACAATTATTCCGTGATGGTGGCATTCCATTGAGGGTTTTTTGTGAGTGGTGGCTAGCAAAAGAGCATGTAGCAAGAATCAATTCTTTTGTTTTTTCATCATTTCCTGGTGCAACGTTTCAAGGGCGCAATGGCTACAGCCTTAGTTATCAGCTACCTTATCGAGAAGGGGCATCTCTTGCAGACATATTTGGTCATTTGGAAATGAACAGAGAAATAGCTGGTATTGCTGAATATAGCATTGGTCAGTCGACGTTGGAGACGATTTTCAATCATTTTGCCGCAAACCAGTAAGGAAGCTTAAGTTTCAACTTAGGCTTCTAATGGTGTAGTAGAAAACGTTAGTTATAACTTATGTGGCGTCTAGCTGCAAACATTTTCAAGATGAAGATAGAATATGTGATTTTGTAAAAAGTAGAGGTTAATTTGATTTTTGACAACTAAACAGGATAATGTGAGATATATGTAATATAGTGGCCCCTCAAATATTTATGGATAGTGAAGACCTGCATTAGACATAATCTGTACCTTTTATGCTTCTATTGCATTACCAGATTTGCAGCTCACTCATATCTTGCCGTTTGGCAGTGAAAAGCCTTTTGACAATAAAAAATTTGTAGCTTCTGGAATTTCTGTTAACCCAGATTCTATACCTCCACTTCTTGTATTACTACTCAAATTAGAGATCTTCAGGTAATGAAAAGGAAATCAGATCAGGAACACAGCAATTGGTCTTTCAATTTGTACACAATTAAAGATGTACATAAATTCTTCATTTACGTGAGGAGCTCCTGTACTCTATGTATGTATTTGAAGTATAATCTAGTCACGCACACATGCTATCTACAAGAATACAAAGGATAACCACTTGCATTGCCTTGTTAGCTACGGTGCAGTCTACTCAATTGTAGTCTTTTGTCATCGAGAAATTAGAAACGAGAAATTCTCTATGGAAGATTTTGTAAGTGAACTTATCTGTAG

Ace file of the UniGene sp_v2.0_unigene3713 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	ATP-binding cassette transporter AtABCA1 [Arabidopsis thaliana]	-	-	74%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	38%
Blast2go	abc transporter a family member 1	-	-	79%

Source	ECs	Term	Type	e value	Identity
Blast2go	Sulfate-transporting ATPase.	EC:3.6.3.25	-	0.0	79%
Blast2go		EC:3.6.3.3-	-	0.0	79%

Source	GOs	Term	Type	Identity
Blast2go	ATP catabolic process	GO:0006200	Biological Process	79%
Blast2go	sulfate transmembrane-transporting ATPase activity	GO:0015419	Molecular Function	79%
Blast2go	amino acid transmembrane transporter activity	GO:0015171	Molecular Function	79%
Blast2go	ATP binding	GO:0005524	Molecular Function	79%
Blast2go	ferric-transporting ATPase activity	GO:0015408	Molecular Function	79%
Blast2go	integral to membrane	GO:0016021	Cellular Component	79%
Blast2go	vacuolar membrane	GO:0005774	Cellular Component	79%

Source	InterPros	Term	Type	Identity
Sma3	Glycoside hydrolase, chitinase active site	IPR001579	-	-
Sma3	ABC transporter-like	IPR003439	-	-
Sma3	AAA+ ATPase domain	IPR003593	-	-
Sma3	EGF-like region, conserved site	IPR013032	-	-
Sma3	ABC transporter, conserved site	IPR017871	-	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: C0PRA1

Fln msg: Unexpected stop codon in the beginning of your sequence, query STOP codon too far from subject stop. Distance to subject end: 223 aas, putative chimeric sequence, your sequence is shorter than subject: 317 - 788

Fln protein: FGHSK*RWWVTSAIYYGQRVLRSLSLFLLLKHVIQQKKQCKGLRSLSLFLLFESHASNFEDEDEDVTAERVRVLSGAAKESIIYLQNLRKIYPSSGNQPEKVAVDSLSFAVQEGECFGFLGTNGAGKSTTLSMLCGEEHPTGGTAYIFGKDILAHPKAAHQHVGYCPQFDALLELLTVREHLELYARIKGVSKEILDQVVEGKLAEFDLWPQANKPSCSLSGGNKRKLSVAIAMIGDPPLVILDEPSTGMDPIAKRFMWDVISRISTRQGKTAVILTTHSMNEAQALCTRIGIMVGGRLCCLGKSSTFENTLWQLS*

Protein Length: 318

Fln nts: CTTTGGCCATAGTAAATAGCGCTGGTGGGTAACAAGCGCTATTTACTATGGCCAAAGGGTCTTACGGAGCCTTTCATTGTTTCTTCTGTTGAAGCATGTGATTCAACAGAAGAAACAATGTAAGGGTCTTAGGAGCCTTTCATTGTTTCTTCTGTTTGAATCACATGCTTCAAATTTTGAGGATGAGGATGAAGATGTAACGGCAGAGAGGGTGAGAGTTCTATCTGGTGCAGCGAAAGAGAGCATAATTTACCTACAGAATCTCAGAAAGATATATCCATCAAGTGGAAATCAGCCAGAGAAGGTTGCAGTTGATTCCTTGAGCTTTGCAGTTCAAGAAGGAGAGTGTTTTGGATTTCTCGGCACAAATGGTGCTGGAAAGAGTACAACACTGTCAATGCTATGTGGCGAAGAGCATCCTACGGGAGGAACTGCATATATTTTTGGCAAGGATATTCTTGCACATCCAAAGGCAGCACATCAGCATGTAGGTTATTGTCCTCAGTTTGATGCCCTTCTAGAGTTACTTACTGTTCGAGAACATCTCGAGCTCTATGCACGGATCAAGGGAGTTTCAAAGGAAATATTAGATCAAGTTGTCGAAGGAAAGCTCGCAGAATTTGATTTATGGCCACAAGCAAACAAACCTTCCTGTTCTCTAAGTGGTGGCAATAAACGCAAGTTATCAGTGGCCATAGCAATGATTGGAGATCCTCCTCTCGTCATTTTAGATGAACCTTCAACGGGTATGGACCCTATTGCAAAGCGATTTATGTGGGATGTCATCTCACGTATATCAACTAGGCAAGGGAAAACAGCAGTTATCCTGACAACACATAGCATGAATGAAGCACAGGCCCTTTGTACACGCATTGGAATTATGGTTGGGGGGCGGCTCTGTTGTCTTGGAAAGTCCTCAACATTTGAAAACACGCTTTGGCAACTATCTTGA___ACTTGAGGTGAAACCTGTGGATGTTAGTGTGGTGGAATTAGATAACTTGTGCAATACTCTACATGATATGTTTTTTGACTTTCCGAGCCATTCTAGAGGTATCTTGAGTGATCTGGAAATTTGTATTGGAGGATCAGAGGCTGGCTCCTCCACTCACATGTCAGAAACTAATTTGTCACATGAAATGATCATTGTTGCGGGGCGTAGACTAGGCAATGAGGAGTATGTGCAAAACCTAATTTCAAACAGCCCTTGCATCGATGGAGTTTTTAGTGAGCAATTATCAGAACAATTATTCCGTGATGGTGGCATTCCATTGAGGGTTTTTTGTGAGTGGTGGCTAGCAAAAGAGCATGTAGCAAGAATCAATTCTTTTGTTTTTTCATCATTTCCTGGTGCAACGTTTCAAGGGCGCAATGGCTACAGCCTTAGTTATCAGCTACCTTATCGAGAAGGGGCATCTCTTGCAGACATATTTGGTCATTTGGAAATGAACAGAGAAATAGCTGGTATTGCTGAATATAGCATTGGTCAGTCGACGTTGGAGACGATTTTCAATCATTTTGCCGCAAACCAGTAAGGAAGCTTAAGTTTCAACTTAGGCTTCTAATGGTGTAGTAGAAAACGTTAGTTATAACTTATGTGGCGTCTAGCTGCAAACATTTTCAAGATGAAGATAGAATATGTGATTTTGTAAAAAGTAGAGGTTAATTTGATTTTTGACAACTAAACAGGATAATGTGAGATATATGTAATATAGTGGCCCCTCAAATATTTATGGATAGTGAAGACCTGCATTAGACATAATCTGTACCTTTTATGCTTCTATTGCATTACCAGATTTGCAGCTCACTCATATCTTGCCGTTTGGCAGTGAAAAGCCTTTTGACAATAAAAAATTTGTAGCTTCTGGAATTTCTGTTAACCCAGATTCTATACCTCCACTTCTTGTATTACTACTCAAATTAGAGATCTTCAGGTAATGAAAAGGAAATCAGATCAGGAACACAGCAATTGGTCTTTCAATTTGTACACAATTAAAGATGTACATAAATTCTTCATTTACGTGAGGAGCTCCTGTACTCTATGTATGTATTTGAAGTATAATCTAGTCACGCACACATGCTATCTACAAGAATACAAAGGATAACCACTTGCATTGCCTTGTTAGCTACGGTGCAGTCTACTCAATTGTAGTCTTTTGTCATCGAGAAATTAGAAACGAGAAATTCTCTATGGAAGATTTTGTAAGTGAACTTATCTGTAG

Fln Alignment:

Contig3713___SNFEDEDEDVTAERVRVLSGAAKESI-----IYLQNLRKIYPSSGNQ---------PEKVAVDSLSFAVQEGECFGFLGTNGAGKSTTLSMLCGEEHPTGGTAYIFGKDILAHPKAAHQH--VGYCPQFDALLELLTVREHLELYARIKGVSKEILDQVVEGKLAEFDLWPQANKPSCSLSGGNKRKLSVAIAMIGDPPLVILDEPSTGMDPIAKRFMWDVISRISTRQGKTAVILTTHSMNEAQALCTRIGIMVGGRLCCLGKS
C0PRA1______________SESSPDDEDVATEESLVRQQVTENTLGSNVAVQVRGLLKTFPGIRNMVGCCKCRKTAPHHAVKGLWINIEKDKLFCLLGPNGAGKTTTINCLTGITPITAGDALIYGHSIKSTSGMSNIRSVMGVCPQFDILWDALSAQEHLYLFAGIKGMPRPAIKSDAENLLSQVKLSEAAHIRAGSYSGGMKRRLSVAIALIGDPKLVFLDEPTTGMDPVTRRYVWDIIE--DAKKGR-AIVLTTHSMEEADILSDRIAIMARGKLRCIGTS

SNPs (tot: 55)

Position	A %	C %	G %	T %	Probability
509		11		88	0.923564
510	88	11			0.923564
511		11		88	0.923564
661	88		11		0.923564
688		11		88	0.923564
694	11		88		0.923564
831		12	87		0.992554
832	87	12			0.992554
833		12		87	0.992554
835	87			12	0.992554
867		14		85	0.993533
1060		10		90	0.90298
1062		10		90	0.90298
1191	10	90			0.90298
1192	90	10			0.90298
1234		54		45	1.0
1323	85	14			0.993533
1324		14	85		0.993533
1327		14	85		0.993533
1328	14	85			0.993533
1329		85		14	0.993533
1330	14	85			0.993533
1361			83	16	0.994512
1363	20	80			0.995493
1364		80	20		0.995493
1367	20			80	0.995493
1368		20	80		0.995493
1369	11	88			0.923564
1370			11	88	0.923564
1371		11		88	0.923564
1374	11			88	0.923564
1376		88		11	0.923564
1378		11		88	0.923564
1381			11	88	0.923564
1543	15	84			0.999986
1627	87	12			0.992554
1628	87	12			0.992554
1630	12			87	0.992554
1773		77	22		0.999999
1774	22	77			0.999999
1775			22	77	0.999999
1778	22	77			0.999999
1781	71		28		1.0
1782	71	28			1.0
1786			28	71	1.0
1787	71		28		1.0
1791		28		71	1.0
1793	28	71			1.0
1794	71	28			1.0
1804	20		80		0.995493
1888	14			85	0.993533
1986		50	50		1.0
2071	75	25			0.996473
2073		66		33	0.997452
2074	66			33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain