SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene3556

Length: 2302 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene3556
CATCCCAAAAATAAAAGCGTCGTGACCTTGGCTGTCACTCAGGTTTTTCTTAATCCCACTTTGTGTTCTTTGTCTTTTTGTTGTCCTGCTCCTTGACCCAACCCAGAGGCGGTGTACTCCTGCCGCTGTTGCAGCACGAAATGACTCCGAGCTATCAAAGTCACATCATTTTCTACCAAATCACACTGGACTAGATCTGCGTTTTGTTCTCAGGCACATTGAATTCAATGGTGAAGAAAGTTTCAAAAGGGTTATCCAGGTGGTGATCTCTGAATGGATAAATGCCTTTAGAATTGCTGACAGATTGAGTAATTTTAAATTCTATTCAGTTGAGTCACGGGTGTGAGATTGGAATGCCAAGGGACACTGGATCTGTGAGGACTGCAAGAATGTGGGTGCCCATCAGGTGTTTAGGCCTTGACTGGTTGGTTCTGCGCACCATCTGGCCGGGATACTTTCATCTGATTCAGCACAGGGAGACCGGGATAGATTTTTCGTAGAAGTGCGGACATTGTCCATCTGGGTATCACAGAATTGATCTAGTGACTACCCAGTTGGCAGCGGAAACTTCTCAATATTTAGATCGACCCCTCTTCATGCATACAATGAGGAGGAGAAACACAGATTTTCGACGTCCAGTGCGGAGGCGATATTCCCGTGGATTGCTGGCAGGTCTTGGGGGATTGCTGATTCTTTTTTTCATTGTAGTTTTGAGCCATCACCGGCAGCCAACTTCTGATGCCAAAAATCCGCAGACAGATGGAGTGAAAAGATGGAGTGTAACAGAAGGCAAAGATGTCACAGAAGAGTTGCTAAGTGCCAAGTCTCTTACAAGGCAGTTGGGGGATCAGATGACCCTTGCTAAAGCATATGTTGTCATTGCAAAAGAAAACAACAATTTGCAACTTGCATGGGATTTTAGTGCTCAGATAAGGGGCTGCCAACTATTACTATCTAAAGCTGCTACCAGGGGCAGTGAGGTTACCATGGAGGAAGCAGAACCCATAATTCGACAGTTATCATTGTTGATTTATCAAGCTAAGGAGCTTCACTATGACAGTGCTACTATGATCATGAAATTGAAGGCTCAGATACAAGCGCTGGAAGATCGTGCAAATGCTGCAACTATACAAAGTACAACATTTGGACAATTGGCTGCAGAAGCAGTGCCCAAGGGTCTTTACTGTTTTGGCACAATGCTATCTAAGGAATGGTCAAAAAATCATAAACTACAAAAATACATGGATCAGCAGAATCATTCACCTAAACTCACAGATAATAAATTATATCACTTTTGTGTGTTTTCTGATAATGTGCTTGCTACATCTGTAGTCGTGAACTCCACGGTTCAGAATGCCGATTATCCAGAACAGCTCGTCTTCCATATTGTCACAGATGAAATCAATCATGGGGCCATGCAAGCTTGGTTTGCTATGAATGACTATAGGGGAGCAACCATAGAAGTGCAGAATGTGGATGACTTTAACTGGCTAAATGCATCTTATGTTCCTGTGTTGAAGCAGCTACGGGACGAGGAAACACAAAGTTACTACTTTAAAGGTTCCTCAGATATGAAGAGTGCAATTAAGTTTCGTAACCCAAAATATCTTTCCATGTTGAACCACTTAAGGTTCTATATTCCTGAAGTATATCCTTCTCTGGAAAAAGTGATCTTTCTGGATGATGATGTAGTAGTTCAGAAAGACTTGACACCACTTTTCTCTATAGACTTGCATGGGAATGTCAATGGGGCGGTTGAAACTTGCCTTGAGACATTTCACCGGTACCATAAATATCTCAATTTCTCCCACCCCATGATTCGTTCTCACTTTGATGCAGATGCTTGTGGATGGGCCTTTGGGATGAATGTGTTTGACCTTGTTACATGGAAGAAGCTCAATGTTACAAGCCGATATCACTATTGGCAAGAGCAGAATGTTGATAGGACACTTTGGAAACTTGGGACTTTGCCTCCAGGCTTGCTTACCTTCTATGGTCTCACTGAGCCACTTGATCGTCGTTGGCATATACTAGGCCTAGGTTATGATCCAAATGTGGACATGCAACTGATAGAAAGTGGAGCTGTTTTGCATTATAATGGGAACATGAAACCATGGTTGAAACTGGCAATGAACAGGTACAAATCTTTTTGGGAAAAGTATGTGAATTACTTGCATCCATTTCTGCAGAAGTGCAACATCCATTAATGTTGGCCTAGCATCATATGCTCTTCTGTTATTAGCCAACCATTACAAGGCTAATTATGATTTTCATAGGTTCTGTAAATGACCCATTGTGCCAG

Ace file of the UniGene sp_v2.0_unigene3556 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Glycosyltransferase, CAZy family GT8 n=2 Tax=Selaginella moellendorffii RepID=D8RE39_SELML	-	-	72%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	21%
Blast2go	cazy family gt8	-	-	80%

Source	ECs	Term	Type	e value	Identity
Blast2go	Transferases, Transferring phosphorous-containing groups, Protein-serine/threonine kinases.	EC:2.7.11.-	-	0.0	80%

Source	GOs	Term	Type	Identity
Blast2go	protein phosphorylation	GO:0006468	Biological Process	80%
Blast2go	ATP binding	GO:0005524	Molecular Function	80%
Blast2go	protein serine/threonine kinase activity	GO:0004674	Molecular Function	80%
Blast2go	transferase activity, transferring glycosyl groups	GO:0016757	Molecular Function	80%

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Glycosyl transferase, family 8	IPR002495	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NYU2

Fln msg: your sequence is longer than subject: 537 - 381

Fln protein: MHTMRRRNTDFRRPVRRRYSRGLLAGLGGLLILFFIVVLSHHRQPTSDAKNPQTDGVKRWSVTEGKDVTEELLSAKSLTRQLGDQMTLAKAYVVIAKENNNLQLAWDFSAQIRGCQLLLSKAATRGSEVTMEEAEPIIRQLSLLIYQAKELHYDSATMIMKLKAQIQALEDRANAATIQSTTFGQLAAEAVPKGLYCFGTMLSKEWSKNHKLQKYMDQQNHSPKLTDNKLYHFCVFSDNVLATSVVVNSTVQNADYPEQLVFHIVTDEINHGAMQAWFAMNDYRGATIEVQNVDDFNWLNASYVPVLKQLRDEETQSYYFKGSSDMKSAIKFRNPKYLSMLNHLRFYIPEVYPSLEKVIFLDDDVVVQKDLTPLFSIDLHGNVNGAVETCLETFHRYHKYLNFSHPMIRSHFDADACGWAFGMNVFDLVTWKKLNVTSRYHYWQEQNVDRTLWKLGTLPPGLLTFYGLTEPLDRRWHILGLGYDPNVDMQLIESGAVLHYNGNMKPWLKLAMNRYKSFWEKYVNYLHPFLQKCNIH*

Protein Length: 538

Fln nts: CATCCCAAAAATAAAAGCGTCGTGACCTTGGCTGTCACTCAGGTTTTTCTTAATCCCACTTTGTGTTCTTTGTCTTTTTGTTGTCCTGCTCCTTGACCCAACCCAGAGGCGGTGTACTCCTGCCGCTGTTGCAGCACGAAATGACTCCGAGCTATCAAAGTCACATCATTTTCTACCAAATCACACTGGACTAGATCTGCGTTTTGTTCTCAGGCACATTGAATTCAATGGTGAAGAAAGTTTCAAAAGGGTTATCCAGGTGGTGATCTCTGAATGGATAAATGCCTTTAGAATTGCTGACAGATTGAGTAATTTTAAATTCTATTCAGTTGAGTCACGGGTGTGAGATTGGAATGCCAAGGGACACTGGATCTGTGAGGACTGCAAGAATGTGGGTGCCCATCAGGTGTTTAGGCCTTGACTGGTTGGTTCTGCGCACCATCTGGCCGGGATACTTTCATCTGATTCAGCACAGGGAGACCGGGATAGATTTTTCGTAGAAGTGCGGACATTGTCCATCTGGGTATCACAGAATTGATCTAGTGACTACCCAGTTGGCAGCGGAAACTTCTCAATATTTAGATCGACCCCTCTTC_-_ATGCATACAATGAGGAGGAGAAACACAGATTTTCGACGTCCAGTGCGGAGGCGATATTCCCGTGGATTGCTGGCAGGTCTTGGGGGATTGCTGATTCTTTTTTTCATTGTAGTTTTGAGCCATCACCGGCAGCCAACTTCTGATGCCAAAAATCCGCAGACAGATGGAGTGAAAAGATGGAGTGTAACAGAAGGCAAAGATGTCACAGAAGAGTTGCTAAGTGCCAAGTCTCTTACAAGGCAGTTGGGGGATCAGATGACCCTTGCTAAAGCATATGTTGTCATTGCAAAAGAAAACAACAATTTGCAACTTGCATGGGATTTTAGTGCTCAGATAAGGGGCTGCCAACTATTACTATCTAAAGCTGCTACCAGGGGCAGTGAGGTTACCATGGAGGAAGCAGAACCCATAATTCGACAGTTATCATTGTTGATTTATCAAGCTAAGGAGCTTCACTATGACAGTGCTACTATGATCATGAAATTGAAGGCTCAGATACAAGCGCTGGAAGATCGTGCAAATGCTGCAACTATACAAAGTACAACATTTGGACAATTGGCTGCAGAAGCAGTGCCCAAGGGTCTTTACTGTTTTGGCACAATGCTATCTAAGGAATGGTCAAAAAATCATAAACTACAAAAATACATGGATCAGCAGAATCATTCACCTAAACTCACAGATAATAAATTATATCACTTTTGTGTGTTTTCTGATAATGTGCTTGCTACATCTGTAGTCGTGAACTCCACGGTTCAGAATGCCGATTATCCAGAACAGCTCGTCTTCCATATTGTCACAGATGAAATCAATCATGGGGCCATGCAAGCTTGGTTTGCTATGAATGACTATAGGGGAGCAACCATAGAAGTGCAGAATGTGGATGACTTTAACTGGCTAAATGCATCTTATGTTCCTGTGTTGAAGCAGCTACGGGACGAGGAAACACAAAGTTACTACTTTAAAGGTTCCTCAGATATGAAGAGTGCAATTAAGTTTCGTAACCCAAAATATCTTTCCATGTTGAACCACTTAAGGTTCTATATTCCTGAAGTATATCCTTCTCTGGAAAAAGTGATCTTTCTGGATGATGATGTAGTAGTTCAGAAAGACTTGACACCACTTTTCTCTATAGACTTGCATGGGAATGTCAATGGGGCGGTTGAAACTTGCCTTGAGACATTTCACCGGTACCATAAATATCTCAATTTCTCCCACCCCATGATTCGTTCTCACTTTGATGCAGATGCTTGTGGATGGGCCTTTGGGATGAATGTGTTTGACCTTGTTACATGGAAGAAGCTCAATGTTACAAGCCGATATCACTATTGGCAAGAGCAGAATGTTGATAGGACACTTTGGAAACTTGGGACTTTGCCTCCAGGCTTGCTTACCTTCTATGGTCTCACTGAGCCACTTGATCGTCGTTGGCATATACTAGGCCTAGGTTATGATCCAAATGTGGACATGCAACTGATAGAAAGTGGAGCTGTTTTGCATTATAATGGGAACATGAAACCATGGTTGAAACTGGCAATGAACAGGTACAAATCTTTTTGGGAAAAGTATGTGAATTACTTGCATCCATTTCTGCAGAAGTGCAACATCCATTAA___TGTTGGCCTAGCATCATATGCTCTTCTGTTATTAGCCAACCATTACAAGGCTAATTATGATTTTCATAGGTTCTGTAAATtACCCATTGTGCCAG

Fln Alignment:

Contig3556___SVVVNSTVQNADYPEQLVFHI-VTDEINHGAMQAWFAMNDYRGATIEVQNVDDFNWLNASYVPVLKQLRDEETQSYYFKGSSDMKSAIKFRNPKYLSMLNHLRFYIPEVYPSLEKVIFLDDDVVVQKDLTPLFSIDLHGNVNGAVETCLETFHRYHKYLN--FSHPMI-RSHFDADACGWAFGM-----------NVFDLVTWKKLNVTSRYHYWQEQNVDRTLWKLGTLPPGLLTFYGLTEPLDRRWHILGLGYDPNVDMQLIESGAVLHYNGNMKPWLKLAMNR
A9NYU2______________AVLVNSTISNSRDPGNIYFYLFIPDDWEEERSRSIFSLL-FRKSNIFIYRHSVIR------------------EKAFASGGEDSKSDFPYFLP----------FYIPKIYQNLRRFIYAVPDIIVKGKVEELFQVNLTNSPVAAVEDCSHNF----EYINAKSSRPWVAQTPYAKNTCILDHSVLLVNVELLAKENFMEVTKWKKLFHTGGRNDQSDQAI-------------MLALNGNYTKLDASWNSRESRFS-GIDSDV----KIFHFDGEKKPWLKQAEGR

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
1855	66		33		0.997452
1856	66	33			0.997452
1858	33			66	0.997452
2250	85			14	0.993533
2251	85		14		0.993533
2256	14		85		0.993533
2257		14	85		0.993533
2259			14	85	0.993533
2260		14		85	0.993533
2264	85		14		0.993533
2265	85			14	0.993533
2266	85	14			0.993533
2268	71	28			1.0
2269		71	14		0.984337
2270	28	57			1.0
2271		28		71	1.0
2283	83			16	0.994512
2290		83		16	0.994512
2291			80	20	0.995493
2293		80		20	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain