SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene3475

Length: 2494 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene3475
GCTGATGACAATGGGGAAGGTGGTACATTTGCATTATATTCTTTATTATGCAGGCATGCCAATATTAGTCTGTTGCCAAACCGTCAGCTTGTTGATGAGGAAGTCTCCACCTACAAATTGGAATATCCCCCTGAGACAAAGAGTGGGTCAAGGGTTAAAAAGATTCTTGAAAATCATAAGAATTTGCATACTGCATTGCTAATCGTGGTTCTACTTGGCACCTGTATGGTTAGTATCCCATCTCCAATAACCATACAGGGGAACACCATGAATATGCAGTTGTTCCTATCACATGCTTCATATTGGTTTGTCTGTTTGCCTTGCAACACTACGGTACACACAGGGTGGGTTCAATATTTGCACCTATTATTGTGGCTTGGCTTCTGTGCATTAGTGCACTTGGTCTCTACAACATCATCCACTGGAACCCACATGTTTATCGAGCCCTCTCGCCATATTATATGTACAAATTTTTAAGGAAGACGAGAAAGGCAGGTTGGATGTCCTTGGGTGGCATTCTGTTGTGCATCACAGGTTCAGAGGCAATGTTTGCTGATCTTGGACACTTCTCCCATTTGTCTATCAAGATTGCTTTCACTTCTATAGTTTATCCTGCTTTGATTTTAGCTTATATGGGTCAAGCTGCTTATCTCTCAAAACATCATCATATGGGAAATAGCTATGCAATTGGTTTCTATGTATCTGTTCCAGAGGCTGTGCGGTGGCCAGTACTAGGAGTTGCGATACTTGCAGCAGTGGTTGGAAGTCAAGCAATCATCACTGGGACATTTTCAATTATCAACCAGAGCTTGGCACTGGGTTGCTTTCCTAGGGTGAAAGTAGTGCATACTTGTGACAAAATACATGGACGGATATACATTCCAGAAATCAACTGGATTTTGATGATCCTATGTCTAGCTGTTACTATTGGTTTTCAAGATACCAAACGAATGGGAAATGCATCTGGTTTAGCTGTCATCACAGTCATGCTAGTTACTACCTGCCTTACATCATTGGTAATAATTATATGCTGGCATAAGAGTATTCTACTAGCAGTATGCTTTCTACTTTTCTTTGGATCTATCGAAGCCCTCTACTTCTCAGCTTCTCTCATTAAGTTTCTAGAAGGGGCCTGGGTACCTATTCTTCTGGCTCTCATATTCATGACTATCATGTATGTGTGGCATTATGGGACAATAAAGAAGTATGACTTCGATTTGCAAAACAAGGTATCCATAAAATGGCTGCTTTCACTTGGTCCTAGTTTGGGGATTGTGCGTGTCCCTGGAATTGGCCTTATGTACACAGAACTTGTCACTGGGATACCTGCAAATTTTACACATTTTGTAACAAATCTTCCAGCATTCCATCAGGTGCTTGTCTTTATATGCATTAAATCGGTGCCAGTGCCGTATGTTCCCGCAGAAGAACGGTTTCTCATTGGTCGCGTAGGGCCTAAAGAGTACAGGTTATACAGATGCATTGTTCGATATGGTTACCGCGATGTCCATGGGGATACTGATGATTTTGAGGATCAGCTTATTCTCAACCTCGGAGAGTTTATTCGTTCAGAAGCAAAACATTCTTGCTCAAGTGACAATTACTCTGCAGACTCTGCAGATGGAAGAATGGCTGTGATTGGAACCCCTGTGCAAGGCTTGGAGAGCATTCTAAGTTACGAAACAACAGAAGAGAGCATACAGTCTGTTAATCTATCCCATCTTCCGTCACAGACCATCCAAAGCATACAGGATCTTATGGAAACTGACTCTCCTCATCTGGCTCGGAGGAGGAAAGTCCGATTTTTGCTGCCTAGCAGTCCTGATATTGATTCTGATGTAAAGGATGAGTTGCAAGAACTCTTTCAGGCTAGGGAATCTGGAGTTGCCTTTATATTAGGGCACTCTTACGTTAAAGCAAAAAATGACTCGTCTTTTTTGAAAAAGCTTGCCATTAATTGTGCGTACAATTTTCTCAGAAAAAATTGCAGGGGACCTTCAGTGGCATTGCGCATTCCCCATATCTCACTTTTAGAGGTTGGTATGGTTTATCTCGTTTGAGTCAGGATCTATATTCCCTGTTGCTGCTTCCAAAGAACATGGGCATCACGTGGCAGTTTTGATCTCTTTTCCTGAGTTTGGTACGGATTATCTTGATTGGGTCAGAGTATCATTGTACCCTAATGCAGTGGCCCATGCACATAGATACAGTCCAAACATTTTCTGATGCGCAGCGTGGAGGCAGTACATATTCTGAAAGGAATTGGTACAAATTTAGCTTAACATCGATGCATTATAAGCATCAACTTTAAAGGAAACAAATTCAGCTTTAAGAGGCCTGAAAATGTAACAACATTATAAAGCTTTCAGGCGCTGCATATTTTCTCCCGACTAGCATTAATTAGAATAGACACATTAGTGAATTTCTTGGAATGTAAGTAGCCTGTTTACATTCTGCTTGGTAACCTTGAAAAAGAATTCTAACATAGGTTTTC

Ace file of the UniGene sp_v2.0_unigene3475 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Potassium transporter HAK2p n=1 Tax=Mesembryanthemum crystallinum RepID=Q94KC6_MESCR	-	-	66%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	92%
Blast2go	potassium transporter 2	-	-	81%

Source	GOs	Term	Type	Identity
Blast2go	potassium ion transmembrane transporter activity	GO:0015079	Molecular Function	81%
Blast2go	plasma membrane	GO:0005886	Cellular Component	81%
Blast2go	potassium ion transmembrane transport	GO:0071805	Biological Process	81%

Source	InterPros	Term	Type	Identity
Sma3	Zinc finger, NF-X1-type	IPR000967	-	-
Sma3	Single-stranded nucleic acid binding R3H	IPR001374	-	-
Sma3	Peptidase aspartic, active site	IPR001969	-	-
Sma3	Multicopper oxidase, copper-binding site	IPR002355	-	-
Sma3	Immunoglobulin/major histocompatibility complex, conserved site	IPR003006	-	-
Sma3	K+ potassium transporter	IPR003855	-	-
Sma3	Zinc finger, C2H2	IPR007087	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3		IPR018519	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A8F8

Fln msg: your sequence is longer than subject: 533 - 250

Fln protein: MYKFLRKTRKAGWMSLGGILLCITGSEAMFADLGHFSHLSIKIAFTSIVYPALILAYMGQAAYLSKHHHMGNSYAIGFYVSVPEAVRWPVLGVAILAAVVGSQAIITGTFSIINQSLALGCFPRVKVVHTCDKIHGRIYIPEINWILMILCLAVTIGFQDTKRMGNASGLAVITVMLVTTCLTSLVIIICWHKSILLAVCFLLFFGSIEALYFSASLIKFLEGAWVPILLALIFMTIMYVWHYGTIKKYDFDLQNKVSIKWLLSLGPSLGIVRVPGIGLMYTELVTGIPANFTHFVTNLPAFHQVLVFICIKSVPVPYVPAEERFLIGRVGPKEYRLYRCIVRYGYRDVHGDTDDFEDQLILNLGEFIRSEAKHSCSSDNYSADSADGRMAVIGTPVQGLESILSYETTEESIQSVNLSHLPSQTIQSIQDLMETDSPHLARRRKVRFLLPSSPDIDSDVKDELQELFQARESGVAFILGHSYVKAKNDSSFLKKLAINCAYNFLRKNCRGPSVALRIPHISLLEVGMVYLV*

Protein Length: 534

Fln nts: GCTGATGACAATGGGGAAGGTGGTACATTTGCATTATATTCTTTATTATGCAGGCATGCCAATATTAGTCTGTTGCCAAACCGTCAGCTTGTTGATGAGGAAGTCTCCACCTACAAATTGGAATATCCCCCTGAGACAAAGAGTGGGTCAAGGGTTAAAAAGATTCTTGAAAATCATAAGAATTTGCATACTGCATTGCTAATCGTGGTTCTACTTGGCACCTGTATGGTTAGTATCCCATCTCCAATAACCATACAGGGGAACACCATGAATATGCAGTTGTTCCTATCACATGCTTCATATTGGTTTGTCTGTTTGCCTTGCAACACTACGGTACACACAGGGTGGGTTCAATATTTGCACCTATTATTGTGGCTTGGCTTCTGTGCATTAGTGCACTTGGTCTCTACAACATCATCCACTGGAACCCACATGTTTATCGAGCCCTCTCGCCATATTAT_-_ATGTACAAATTTTTAAGGAAGACGAGAAAGGCAGGTTGGATGTCCTTGGGTGGCATTCTGTTGTGCATCACAGGTTCAGAGGCAATGTTTGCTGATCTTGGACACTTCTCCCATTTGTCTATCAAGATTGCTTTCACTTCTATAGTTTATCCTGCTTTGATTTTAGCTTATATGGGTCAAGCTGCTTATCTCTCAAAACATCATCATATGGGAAATAGCTATGCAATTGGTTTCTATGTATCTGTTCCAGAGGCTGTGCGGTGGCCAGTACTAGGAGTTGCGATACTTGCAGCAGTGGTTGGAAGTCAAGCAATCATCACTGGGACATTTTCAATTATCAACCAGAGCTTGGCACTGGGTTGCTTTCCTAGGGTGAAAGTAGTGCATACTTGTGACAAAATACATGGACGGATATACATTCCAGAAATCAACTGGATTTTGATGATCCTATGTCTAGCTGTTACTATTGGTTTTCAAGATACCAAACGAATGGGAAATGCATCTGGTTTAGCTGTCATCACAGTCATGCTAGTTACTACCTGCCTTACATCATTGGTAATAATTATATGCTGGCATAAGAGTATTCTACTAGCAGTATGCTTTCTACTTTTCTTTGGATCTATCGAAGCCCTCTACTTCTCAGCTTCTCTCATTAAGTTTCTAGAAGGGGCCTGGGTACCTATTCTTCTGGCTCTCATATTCATGACTATCATGTATGTGTGGCATTATGGGACAATAAAGAAGTATGACTTCGATTTGCAAAACAAGGTATCCATAAAATGGCTGCTTTCACTTGGTCCTAGTTTGGGGATTGTGCGTGTCCCTGGAATTGGCCTTATGTACACAGAACTTGTCACTGGGATACCTGCAAATTTTACACATTTTGTAACAAATCTTCCAGCATTCCATCAGGTGCTTGTCTTTATATGCATTAAATCGGTGCCAGTGCCGTATGTTCCCGCAGAAGAACGGTTTCTCATTGGTCGCGTAGGGCCTAAAGAGTACAGGTTATACAGATGCATTGTTCGATATGGTTACCGCGATGTCCATGGGGATACTGATGATTTTGAGGATCAGCTTATTCTCAACCTCGGAGAGTTTATTCGTTCAGAAGCAAAACATTCTTGCTCAAGTGACAATTACTCTGCAGACTCTGCAGATGGAAGAATGGCTGTGATTGGAACCCCTGTGCAAGGCTTGGAGAGCATTCTAAGTTACGAAACAACAGAAGAGAGCATACAGTCTGTTAATCTATCCCATCTTCCGTCACAGACCATCCAAAGCATACAGGATCTTATGGAAACTGACTCTCCTCATCTGGCTCGGAGGAGGAAAGTCCGATTTTTGCTGCCTAGCAGTCCTGATATTGATTCTGATGTAAAGGATGAGTTGCAAGAACTCTTTCAGGCTAGGGAATCTGGAGTTGCCTTTATATTAGGGCACTCTTACGTTAAAGCAAAAAATGACTCGTCTTTTTTGAAAAAGCTTGCCATTAATTGTGCGTACAATTTTCTCAGAAAAAATTGCAGGGGACCTTCAGTGGCATTGCGCATTCCCCATATCTCACTTTTAGAGGTTGGTATGGTTTATCTCGTTTGA___GTCAGGATCTATATTCCCTGTTGCTGCTTCCAAAGAACATGGGCATCACGTGGCAGTTTTGATCTCTTTTCCTGAGTTTGGTACGGATTATCTTGATTGGGTCAGAGTATCATTGTACCCTAATGCAGTGGCCCATGCACATAGATACAGTCCAAACATTTTCTGATGCGCAGCGTGGAGGCAGTACATATTCTGAAAGGAATTGGTACAAATTTAGCTTAACATCGATGCATTATAAGCATCAACTTTAAAGGAAACAAATTCAGCTTTAAGAGGCCTGAAAATGTAACAACATTATAAAGCTTTCAGGCGCTGCATATTTTCTCCCGACTAGCATTAATTAGAATAGACACATTAGTGAATTTCTTGGAATGTAAGTAGCCTGTTTACATTCTGCTTGGTAACCTTGAAAAAGAATTCTAACATAGGTTTTC

Fln Alignment:

Contig3475___MYTELVTGIPANFTHFVTNLPAFHQVLVFICIKSVPVPYVPAEERFLIGRVGPKEYRLYRCIVRYGYRDVHGDTDDFEDQLILNLGEFIRSEAKHSCSSDNYSADSADGRMAVIGTPVQGLESILSYETTEESIQSVNLSHLPSQTIQSIQDLMETDSPHLARRRKVRFLLPSSPDIDSDVKDELQELFQARESGVAFILGHSYVKAKNDSSFLKKLAINCAYNFLRKNCRGPSVALRIPHISLLEVGMVYLV
D5A8F8______________MYTELVTGIPANFTHFVTNLPAFHQVLIFICIKSVPVPYVPPEERFLIGRVGPKEYRLYRCIVRYGYRDVHGDTDHFEDQLILNLGEFIRSEAKHSCSSDSYSAD---GRMAVIGTPVHGMESILSYETAEESIQSVNLSYLGSQTIQSIQDLIETDSPHLARRKKVRFLLPSSPDIDPDVKDELQELFQAREAGIAFILGHSYVKAKNDSSFLKKLAINAGYNFLRKNCRGPSVALRIPHISLLEVGMVYLV

SNPs (tot: 14)

Position	A %	C %	G %	T %	Probability
2032	90	10			0.90298
2036	88			11	0.923564
2061	84			15	0.999986
2062			15	84	0.999986
2066	15		84		0.999986
2067		15	84		0.999986
2068	15			84	0.999986
2069			15	84	0.999986
2073	15		84		0.999986
2074			15	84	0.999986
2076			84	15	0.999986
2078		15		84	0.999986
2081		15		84	0.999986
2130	90			10	0.90298

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain