SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene3447

Length: 3031 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene3447
AATGTAACCCATTACAGTGATTTCGCCGATTGTTTTATCTGTAAAGTGGTATTTTCGCAGTGCTTACCCGCGCGCGAAGATTCTGGTAAGGGTGCACAGCATCGGCGAACTTGTTCATACAATAATCCCATTTCTCATGGAGTTGGTGTGCATTGCCGCGAGAACGAAAAGTTAGCTGTGGATCAGGACCATTGCCGCCTCCCTTGCACCTTTTTTTCCTGTAAGTTACAGAGTCTGAGGAGTAAGGGAGCATATCTGCTCTTTCCAGGATAAATATTTTAAGAAATGTCTGTACTGAAAAACCTACGCGGCCTTTTTGGTTAAATTTACCAGCTCGAAGGTGTTGAGGGAGTTCGCTGCCGCCCCGGTTATTTTCTTGGACTCTTTGAGTGATCATCAAAGTAGAGAGGAGCCAACTTGGAGCATTTTTAAGAATTGAGTTTATTCATGTGGCAAGTGTAGACCCAAGCAACCAATATCTCTGCAACATAACTGTAGAGATGCAAAGATGAGTACTCTTTTTATTGCTATTTTGGTATCTGTGCTATTTTCCCTCGCTTTTGGTAAAATTAAGAACAGGAAACTGCGTGGGGTGCCTGTCAAATCTGGAGATACTGGTTTCACCATTCGCAACAGTAAATTTCCTACTCTGTTAGAAGTACCTTTTGAAGGAGCTTCAACTCTTGCAGCCCTCTTTGAGCAGTCATGTAAACGCCATGAACATTTTCCCTTGTTTGGGACTCGAAAACTTCTTGGCAGACAAAGAGAAGTCAGTGAAGATGGAAAACCTTGTGAAAAGTTGCATCTGGGAAACTATGAATGGATAACCTATGGTTCAGCATTTAAGCGTGCTTGCCACTTTGCGTCAGGACTGATTGAACAAGGCCATAAAAGAGGACAGCGAGCTGCAATTTTTGCTGAAACTCGAGCAGAGTGGCAAATTGCTCTTCAGGGTTGCTTCCGATGCAATATCACAGTGGTTAGTCTTTATCCTTCGTTAGATGGTGAAGCTCTTGCTCACTCATTAAATGAGACGGAAGTGTCAACAGTCATATGCGACCCCAAGCAGCTGCAGAAACTTTTTGATATAAGCAGACAATTAGAGACTGTAGAACGCGTTGTTTACATGGACGACACAGGACTTATAGACAGTCCTCTTTTATCAAGAACTGATAGCGGGTTCACATTTTTATCCTTCTCTGAAATTGAGAGGCATGGGCGGCAGAATCGTGCTGAACCAAATTTACCTGTACCAGCTGACATTGCAGTTATCATGTATACAAGTGGCAGCGCAGGATTGCCTAAGGGAGTTATGATGACTCATGGAAATGTTGTAGCAACAGTTGCTGCAGTAACAACAATTTTACCTGATCTCACAGATGAAGATAAATATTTGGCATATCTACCATTAGCACATATCCTTGAATTAGCAGCCGAGTGTACAATGATTTCTGCAGGCTGTCCAATTGGTTATGGTACACCTTTAACAATACTTGATAAGTCAAACATGATCAAAAGAGGAACCAAAGGGGATGCTACAATGCTCGGCCCTACGCTTATGGCTGCAGTGCCAGCGATATTAGACCGTCTTCGAGATGGTGTAAGGGAAAAGGTCCAATCAAAGAGCAACATATCAAGGAAACTCTTTGAAATTTCGTATAGGCGTAGATTGGCGGCTATTGAAGGAAGCTGGCAAGGTGCATGGGGTCTAGAGAAGTTTCTATGGGATGAACTGGTATTTAGAAAGATACGTGCAATTCTTGGAGGGCATGTTCGCGGTTTACTTTCTGGCGGAGCTCCTCTATCTGGAGAAACTCAGCGGTTTGTGAACATATGCCTTGGGGCACCGATTTTGCAAGGATATGGTCTTACAGAAACATGTGCTGGAGCAACATTTTCAGAGTGGGATGATTCAACTGTTGGTCGTGTTGGTCCTCCACTTCCTAATTGCTATGTTAAGCTAATTGATTGGGAGGAAGGTGGCTATTTGACAACTGATTCACCAATGCCTCAAGGAGAGATTGTTATTGGTGGAGCAAATGTAACTTTGGGATATTTCAAAAGAAAGGCTGAAACTAGTCAGTTCTACAAGATGGATGAGAGAGGAATACGATGGTTTTATTCTGGCGATATAGGGAAATTCCACCCTGATGGTTGTCTTGAGATTGTTGACCGTAAGAAGAACATAGTAAAGCTTCAGCGTGGAGAACAGGTTCCCTTAGGAAAGGTTGAAGCAGTTTTGATAATGAGCCAATATGTAGAACAGATAATGTTGCATGCAGATCATCTCCATAGTTACTGTGTGGCCCTTGTGGTCCCATCCCAAAAAGTTCTTGAAAGTTGGGCCCAAAAAGAAATGCTTATTTACAATGATTTTGAAGATCTGTGCCAAAAGAAAGAAGCAATCAAAGAAATTCACATGTCAATTCTAAAGGTTGCAAAAGAGGCAAATCTCAACAACTTTGAAATTCCGAAGAAGATTAAATTACTTTCACACCCATGGACGCCTGAAAGCAACCTTGTTACAACAGCACTCAAATTGAAGCGAGAGGCTTTAACGACTACCTTTGAACAAGAACTAAATGAGTTGTACCAGTCAAGTTAGCTGTTTCTTGAGTGGTTGTCTGTAAGATTAAAGGGAGTGAGATATCCTATTTCGTGCTGTAACTTACGATAACATTGATTTTGTTTTTTACTTCATCTAATCATTTGAAAGCTATATGTATGATTTATATGTCTGATAAGTTTAAAGGGTGCTTTCATGCTTCCTTTACTGCTCGAGGCGCGACTTTTCTTAACATTTTTATTTGTAGCAGATTATGTTGTGTTTATTGTACATACAGGTCATAAAACGGCATATGAGAAATGAGAGCAGTGTAATAAGAGGGAAAGTAAGAAATAAAATGTGCTTGTGCATGCCTCGATTGCGATGTACGCTCAAATGACCATTAAGAATGTTTGTGATATGTTGTAACTAATATTGAACATATGACGATGACAAGAGATATTTTTGGATTCAGTTTCAGACATT

Ace file of the UniGene sp_v2.0_unigene3447 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	long chain acyl-CoA synthetase 9 [Arabidopsis thaliana] sp\|Q9CAP8.1\|LACS9_ARATH RecName: Full=Long chain acyl-CoA synthetase 9, chloroplastic gb\|AAG51668.1\|AC010704_12 putative acyl-CoA synthetase; 62297-59022 [Arabidopsis thaliana] gb\|AAM28876.1\|AF503759	-	-	60%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	69%
Blast2go	long chain acyl- synthetase 9	-	-	76%

Source	ECs	Term	Type	e value	Identity
Blast2go	Long-chain-fatty-acid--CoA ligase.	EC:6.2.1.3	-	0.0	76%

Source	KEGGs	Term	Type	Identity
Blast2go	Fatty acid metabolism	00071		76%
Blast2go	Metabolic pathways	01100		76%
Blast2go	Phosphoprotein phosphatase.	EC:3.1.3.16	-	76%

Source	GOs	Term	Type	Identity
Blast2go	fatty acid biosynthetic process	GO:0006633	Biological Process	76%
Blast2go	long-chain fatty acid-CoA ligase activity	GO:0004467	Molecular Function	76%
Blast2go	phosphoprotein phosphatase activity	GO:0004721	Molecular Function	76%
Blast2go	chloroplast envelope	GO:0009941	Cellular Component	76%
Blast2go	membrane	GO:0016020	Cellular Component	76%

Source	InterPros	Term	Type	e value	Identity
Sma3	AMP-dependent synthetase/ligase	IPR000873	-	0.0	-
Sma3	Lipocalin	IPR002345	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ACR7

Fln msg: your sequence is longer than subject: 699 - 197

Fln protein: MSTLFIAILVSVLFSLAFGKIKNRKLRGVPVKSGDTGFTIRNSKFPTLLEVPFEGASTLAALFEQSCKRHEHFPLFGTRKLLGRQREVSEDGKPCEKLHLGNYEWITYGSAFKRACHFASGLIEQGHKRGQRAAIFAETRAEWQIALQGCFRCNITVVSLYPSLDGEALAHSLNETEVSTVICDPKQLQKLFDISRQLETVERVVYMDDTGLIDSPLLSRTDSGFTFLSFSEIERHGRQNRAEPNLPVPADIAVIMYTSGSAGLPKGVMMTHGNVVATVAAVTTILPDLTDEDKYLAYLPLAHILELAAECTMISAGCPIGYGTPLTILDKSNMIKRGTKGDATMLGPTLMAAVPAILDRLRDGVREKVQSKSNISRKLFEISYRRRLAAIEGSWQGAWGLEKFLWDELVFRKIRAILGGHVRGLLSGGAPLSGETQRFVNICLGAPILQGYGLTETCAGATFSEWDDSTVGRVGPPLPNCYVKLIDWEEGGYLTTDSPMPQGEIVIGGANVTLGYFKRKAETSQFYKMDERGIRWFYSGDIGKFHPDGCLEIVDRKKNIVKLQRGEQVPLGKVEAVLIMSQYVEQIMLHADHLHSYCVALVVPSQKVLESWAQKEMLIYNDFEDLCQKKEAIKEIHMSILKVAKEANLNNFEIPKKIKLLSHPWTPESNLVTTALKLKREALTTTFEQELNELYQSS*

Protein Length: 700

Fln nts: AATGTAACCCATTACAGTGATTTCGCCGATTGTTTTATCTGTAAAGTGGTATTTTCGCAGTGCTTACCCGCGCGCGAAGATTCTGGTAAGGGTGCACAGCATCGGCGAACTTGTTCATACAATAATCCCATTTCTCATGGAGTTGGTGTGCATTGCCGCGAGAACGAAAAGTTAGCTGTGGATCAGGACCATTGCCGCCTCCCTTGCACCTTTTTTTCCTGTAAGTTACAGAGTCTGAGGAGTAAGGGAGCATATCTGCTCTTTCCAGGATAAATATTTTAAGAAATGTCTGTACTGAAAAACCTACGCGGCCTTTTTGGTTAAATTTACCAGCTCGAAGGTGTTGAGGGAGTTCGCTGCCGCCCCGGTTATTTTCTTGGACTCTTTGAGTGATCATCAAAGTAGAGAGGAGCCAACTTGGAGCATTTTTAAGAATTGAGTTTATTCATGTGGCAAGTGTAGACCCAAGCAACCAATATCTCTGCAACATAACTGTAGAGATGCAAAG_-_ATGAGTACTCTTTTTATTGCTATTTTGGTATCTGTGCTATTTTCCCTCGCTTTTGGTAAAATTAAGAACAGGAAACTGCGTGGGGTGCCTGTCAAATCTGGAGATACTGGTTTCACCATTCGCAACAGTAAATTTCCTACTCTGTTAGAAGTACCTTTTGAAGGAGCTTCAACTCTTGCAGCCCTCTTTGAGCAGTCATGTAAACGCCATGAACATTTTCCCTTGTTTGGGACTCGAAAACTTCTTGGCAGACAAAGAGAAGTCAGTGAAGATGGAAAACCTTGTGAAAAGTTGCATCTGGGAAACTATGAATGGATAACCTATGGTTCAGCATTTAAGCGTGCTTGCCACTTTGCGTCAGGACTGATTGAACAAGGCCATAAAAGAGGACAGCGAGCTGCAATTTTTGCTGAAACTCGAGCAGAGTGGCAAATTGCTCTTCAGGGTTGCTTCCGATGCAATATCACAGTGGTTAGTCTTTATCCTTCGTTAGATGGTGAAGCTCTTGCTCACTCATTAAATGAGACGGAAGTGTCAACAGTCATATGCGACCCCAAGCAGCTGCAGAAACTTTTTGATATAAGCAGACAATTAGAGACTGTAGAACGCGTTGTTTACATGGACGACACAGGACTTATAGACAGTCCTCTTTTATCAAGAACTGATAGCGGGTTCACATTTTTATCCTTCTCTGAAATTGAGAGGCATGGGCGGCAGAATCGTGCTGAACCAAATTTACCTGTACCAGCTGACATTGCAGTTATCATGTATACAAGTGGCAGCGCAGGATTGCCTAAGGGAGTTATGATGACTCATGGAAATGTTGTAGCAACAGTTGCTGCAGTAACAACAATTTTACCTGATCTCACAGATGAAGATAAATATTTGGCATATCTACCATTAGCACATATCCTTGAATTAGCAGCCGAGTGTACAATGATTTCTGCAGGCTGTCCAATTGGTTATGGTACACCTTTAACAATACTTGATAAGTCAAACATGATCAAAAGAGGAACCAAAGGGGATGCTACAATGCTCGGCCCTACGCTTATGGCTGCAGTGCCAGCGATATTAGACCGTCTTCGAGATGGTGTAAGGGAAAAGGTCCAATCAAAGAGCAACATATCAAGGAAACTCTTTGAAATTTCGTATAGGCGTAGATTGGCGGCTATTGAAGGAAGCTGGCAAGGTGCATGGGGTCTAGAGAAGTTTCTATGGGATGAACTGGTATTTAGAAAGATACGTGCAATTCTTGGAGGGCATGTTCGCGGTTTACTTTCTGGCGGAGCTCCTCTATCTGGAGAAACTCAGCGGTTTGTGAACATATGCCTTGGGGCACCGATTTTGCAAGGATATGGTCTTACAGAAACATGTGCTGGAGCAACATTTTCAGAGTGGGATGATTCAACTGTTGGTCGTGTTGGTCCTCCACTTCCTAATTGCTATGTTAAGCTAATTGATTGGGAGGAAGGTGGCTATTTGACAACTGATTCACCAATGCCTCAAGGAGAGATTGTTATTGGTGGAGCAAATGTAACTTTGGGATATTTCAAAAGAAAGGCTGAAACTAGTCAGTTCTACAAGATGGATGAGAGAGGAATACGATGGTTTTATTCTGGCGATATAGGGAAATTCCACCCTGATGGTTGTCTTGAGATTGTTGACCGTAAGAAGAACATAGTAAAGCTTCAGCGTGGAGAACAGGTTCCCTTAGGAAAGGTTGAAGCAGTTTTGATAATGAGCCAATATGTAGAACAGATAATGTTGCATGCAGATCATCTCCATAGTTACTGTGTGGCCCTTGTGGTCCCATCCCAAAAAGTTCTTGAAAGTTGGGCCCAAAAAGAAATGCTTATTTACAATGATTTTGAAGATCTGTGCCAAAAGAAAGAAGCAATCAAAGAAATTCACATGTCAATTCTAAAGGTTGCAAAAGAGGCAAATCTCAACAACTTTGAAATTCCGAAGAAGATTAAATTACTTTCACACCCATGGACGCCTGAAAGCAACCTTGTTACAACAGCACTCAAATTGAAGCGAGAGGCTTTAACGACTACCTTTGAACAAGAACTAAATGAGTTGTACCAGTCAAGTTAG___CTGTTTCTTGAGTGGTTGTCTGTAAGATTAAAGGGAGTGAGATATCCTATTTCGTGCTGTAACTTACGATAACATTGATTTTGTTTTTTACTTCATCTAATCATTTGAAAGCTATATGTATGATTTATATGTCTGATAAGTTTAAAGGGTGCTTTCATGCTTCCTTTACTGCTCGAGGCGCGACTTTTCTTAACATTTTTATTTGTAGCAGATTATGTTGTGTTTATTGTACATACAGGTCATAAAACGGCATATGAGAAATGAGAGCAGTGTAATAAGAGGGAAAGTAAGAAATAAAATGTGCTTGTGCATGCCTCGATTGCGATGTACGCTCAAATGACCATTAAGAATGTTTGTGATATGTTGTAACTAATATTGAACATATGACGATGACAAGAGATATTTTTGGATTCAGTTTCAGACATT

Fln Alignment:

Contig3447___MPQGEIVIGGANVTLGYFKRKAETSQFYKMDERGIRWFYSGDIGKFHPDGCLEIVDRKKNIVKLQRGEQVPLGKVEAVLIMSQYVEQIMLHADHLHSYCVALVVPSQKVLESWAQKEMLIYNDFEDLCQKKEAIKEIHMSILKVAKEANLNNFEIPKKIKLLSHPWTPESNLVTTALKLKREALTTTFEQELNELY
D5ACR7______________MPRGEIVIGGPHVTLGYFKNKEKTDESYKVDERGMRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYVSLGKVEAALSVCHYLDNFMLHADPFHSYCVALVVPSRQALESWAQKEGIIYKDFSDLCQKSEAIKEVQSSILEVGKSAKLDKFEIPSKIKLLPDPWTPETGLVTAALKLKREVIKKTFAEELNKLY

SNPs (tot: 27)

Position	A %	C %	G %	T %	Probability
2506	10		90		0.90298
2509	90			10	0.90298
2521		88	11		0.923564
2522	11			88	0.923564
2525			11	88	0.923564
2527		11		88	0.923564
2528	11			88	0.923564
2529	11			88	0.923564
2532	88		11		0.923564
2535	11			88	0.923564
2537			11	88	0.923564
2541		11	88		0.923564
2650		16	83		0.994512
2652			83	16	0.994512
2708	75			25	0.996473
2714		75		25	0.996473
2795		33		66	0.997452
2829	50		50		1.0
2838		25	75		0.996473
2868		50		50	1.0
2911	50	50			1.0
2920	75			25	0.996473
2922	75	25			0.996473
2923	75	25			0.996473
2924		25	75		0.996473
2925	25			75	0.996473
2948	33	66			0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain