SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene3379

Length: 2207 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene3379
GTGCAGCTGCTGCAGCAGCACCCGCGTTCTGGGGTTCTCCGGAACCACACGATACAGAAGCCTTCAACCACAGGGTTATATACAGCAAGGGCCTCTGCTGTATGTGGACTTTATAACCAAAGTAAGGGCAGGAAGAAATTACCTTTTCTCTCGATACCATTGCAATCCGAGTATGTTGAGAGGAGGCATGGTATCGAGAGAAGGCAGAGGTCTAATGGTTTTAGAAGAAAGGCTCAGCAATGGAGGTTTACAAAAACAGCTTTTGGGTATTACCCATAGAAGAGTGATGCACAATATTTGTAGGGTAAAAAACCGAAGGCATATGGCCTTGTGCTTTAACAATGGTGACCGACATGATAAGCTTGCAGAAAATTCAGTGTCCTGCACAACGTTCAATATTCTTGCGCCAATATACAAGCGTATCAATGGCGAGGACAGTCGAGAAAGCCAATTCAGAGACTATTGGATCAGCCGAAACAAGAGTATACTTGATATGCTGCTCCTGGAAAGATCTTCTGTCATTTGTTTACAGGAGTTCTGGCTTCGGAATGAAGAGCTTGTGGAGATGTATGAACAGAAATTCCATGATGGTGGATATGAGATCTATAAACTTGGACGCACCAATAATCGTGGAGATGGTCTTTTCACTGCAGTGAGAAAGGACCATTTCAAGGTTGTGAACCAGCGGGAACTTCTCTTCCATGACTTCGGAGACCGTGTAGCTCAGTTATTGCACCTCCGGTCTGTAGTTCCATCTTTACAGAGTGAAACGGGTGGTGTTCCATACGAAGCTCTTGTTGTTAACACCCATTTGCTATTCCCCCACAATTCTGATTTCTGCCTTGTTCGCCTCCGTCAGGTATACAAGATCTTAGAGTATTTGGAGGCCTTTAAGGCAGATTATAAGCTTCCTCCAATCCCTGTTATTCTCTGTGGGGACTGGAATGGGAGCAAACGTGGACACGTATACAAGTTTTTACGGTCTCAAGGATTTGTCTCATCTTATGATGCTGCTCATAATTACAGTGACAATGATAAAGATGCTCATAAGTGGATCAGCCACAGGAATCACCGAGGAAACATCTGTGGGGTGGATTTCATTTGGCTTCTTAATCCCAATAAACGTCGGAAGCTTTTAAGGACTAGCTGGATGGAAGCAGTTTTTGGAATTATTAAGTCCAAATTGAAAGAAGCTGGTTTTAATGATTTAGATGCCTTTTGCTTCTTTAAATCAGCGAAGAGATTTGAGGATTATTTGACACTGAAGGACTTCGGTCAGGGACTTCAACAGCTAGGGCTCACAGAACAGTGTTCAGAGGGTCTTACAACGGAGGAGATTGAAGACTTGATGGTGGCAGCTGACCTTGATGGAAATGGTATAATAGACAGTTGGGAATTTGAGAAATTGATGGTGGCACAATCCATTGACCGGAGTTCTGTTATTCATTCGGAAGGAACAAATGTTGCAGCAGTGAAATTGTCAGCACCCTATTTTCCTGGGAATAGTGAAGGACCACTTAAGAAATATCAAAATGGCTCTACAACTTCGGAACATAATAAACACTCACTTTTAATGCAAACAATAGAGTCTGAATCAGGGACTCAATTGATGAAGTTTGATGCAGATCCAGATATGCAGGATCCTTGTTACCTTCAAGAAACAGAAATCGGTTTTGATGTGAAGGGTGCATCTTTGTTTCCTTCAGAGGTGGAACAGGGCATCTGGCCTGAAAACTATCTCATGTCTGACCATGCTCTACTAAGTGCTGTTTTTTCACCGGTAAAGATACCCCCGTGTGACAAAGCGAACCTTTGACCAGAGTGTGGTTCTCCAAAAATGGGTTTTGTAACTGTAGAAAAAGGAAAAATGAATTTATCATGACATACTATGATAACACATTGTTCATCAAAATCCCAGAATTGTAGTTCTCCAAAATACAGTGATTAATCATCAGCAAGCAGTAGTCTTGGTGTCTGAAGTTTACAACACTTCATTACTTGACGAAGTTATATATCTAGTCTCGACTTCATTCTTTTCTGAGATCAGAATGGTTTTAGTCAAAGTTTATATCTGGAAATTTTGCAATCAAAAGATGTACCATTGCCAAGTTTCTCACCAAAAATCAACATATGTAATCGTAGAACACATCAAATCTCTGTAATTTTAGGTCTCTCAAATCACAACACTGAATTTTTATATGTTACGA

Ace file of the UniGene sp_v2.0_unigene3379 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Carbon catabolite repressor protein, putative n=1 Tax=Ricinus communis RepID=B9RMY1_RICCO	-	-	51%
FL-Next	sp=Uncharacterized calcium-binding protein At1g02270; Arabidopsis thaliana (Mouse-ear cress).	-	-	50%
Blast2go	uncharacterized calcium-binding protein at1g02270-like	-	-	65%

Source	GOs	Term	Type	Identity
Blast2go	response to cold	GO:0009409	Biological Process	65%
Blast2go	catalytic activity	GO:0003824	Molecular Function	65%
Blast2go	calcium ion binding	GO:0005509	Molecular Function	65%
Blast2go	nucleus	GO:0005634	Cellular Component	65%

Source	InterPros	Term	Type	Identity
Sma3	EF-hand-like domain	IPR011992	-	-
Sma3	EF-hand	IPR018248	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	EF-HAND 2	IPR018249	-	-
Sma3	Endonuclease/exonuclease/phosphatase	IPR005135	-	-
Sma3		IPR000173	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: sp_plants

Fln subject: O81916

Fln msg: your sequence is longer than subject: 548 - 484

Fln protein: MLRGGMVSREGRGLMVLEERLSNGGLQKQLLGITHRRVMHNICRVKNRRHMALCFNNGDRHDKLAENSVSCTTFNILAPIYKRINGEDSRESQFRDYWISRNKSILDMLLLERSSVICLQEFWLRNEELVEMYEQKFHDGGYEIYKLGRTNNRGDGLFTAVRKDHFKVVNQRELLFHDFGDRVAQLLHLRSVVPSLQSETGGVPYEALVVNTHLLFPHNSDFCLVRLRQVYKILEYLEAFKADYKLPPIPVILCGDWNGSKRGHVYKFLRSQGFVSSYDAAHNYSDNDKDAHKWISHRNHRGNICGVDFIWLLNPNKRRKLLRTSWMEAVFGIIKSKLKEAGFNDLDAFCFFKSAKRFEDYLTLKDFGQGLQQLGLTEQCSEGLTTEEIEDLMVAADLDGNGIIDSWEFEKLMVAQSIDRSSVIHSEGTNVAAVKLSAPYFPGNSEGPLKKYQNGSTTSEHNKHSLLMQTIESESGTQLMKFDADPDMQDPCYLQETEIGFDVKGASLFPSEVEQGIWPENYLMSDHALLSAVFSPVKIPPCDKANL*

Protein Length: 549

Fln nts: GTGCAGCTGCTGCAGCAGCACCCGCGTTCTGGGGTTCTCCGGAACCACACGATACAGAAGCCTTCAACCACAGGGTTATATACAGCAAGGGCCTCTGCTGTATGTGGACTTTATAACCAAAGTAAGGGCAGGAAGAAATTACCTTTTCTCTCGATACCATTGCAATCCGAGT_-_ATGTTGAGAGGAGGCATGGTATCGAGAGAAGGCAGAGGTCTAATGGTTTTAGAAGAAAGGCTCAGCAATGGAGGTTTACAAAAACAGCTTTTGGGTATTACCCATAGAAGAGTGATGCACAATATTTGTAGGGTAAAAAACCGAAGGCATATGGCCTTGTGCTTTAACAATGGTGACCGACATGATAAGCTTGCAGAAAATTCAGTGTCCTGCACAACGTTCAATATTCTTGCGCCAATATACAAGCGTATCAATGGCGAGGACAGTCGAGAAAGCCAATTCAGAGACTATTGGATCAGCCGAAACAAGAGTATACTTGATATGCTGCTCCTGGAAAGATCTTCTGTCATTTGTTTACAGGAGTTCTGGCTTCGGAATGAAGAGCTTGTGGAGATGTATGAACAGAAATTCCATGATGGTGGATATGAGATCTATAAACTTGGACGCACCAATAATCGTGGAGATGGTCTTTTCACTGCAGTGAGAAAGGACCATTTCAAGGTTGTGAACCAGCGGGAACTTCTCTTCCATGACTTCGGAGACCGTGTAGCTCAGTTATTGCACCTCCGGTCTGTAGTTCCATCTTTACAGAGTGAAACGGGTGGTGTTCCATACGAAGCTCTTGTTGTTAACACCCATTTGCTATTCCCCCACAATTCTGATTTCTGCCTTGTTCGCCTCCGTCAGGTATACAAGATCTTAGAGTATTTGGAGGCCTTTAAGGCAGATTATAAGCTTCCTCCAATCCCTGTTATTCTCTGTGGGGACTGGAATGGGAGCAAACGTGGACACGTATACAAGTTTTTACGGTCTCAAGGATTTGTCTCATCTTATGATGCTGCTCATAATTACAGTGACAATGATAAAGATGCTCATAAGTGGATCAGCCACAGGAATCACCGAGGAAACATCTGTGGGGTGGATTTCATTTGGCTTCTTAATCCCAATAAACGTCGGAAGCTTTTAAGGACTAGCTGGATGGAAGCAGTTTTTGGAATTATTAAGTCCAAATTGAAAGAAGCTGGTTTTAATGATTTAGATGCCTTTTGCTTCTTTAAATCAGCGAAGAGATTTGAGGATTATTTGACACTGAAGGACTTCGGTCAGGGACTTCAACAGCTAGGGCTCACAGAACAGTGTTCAGAGGGTCTTACAACGGAGGAGATTGAAGACTTGATGGTGGCAGCTGACCTTGATGGAAATGGTATAATAGACAGTTGGGAATTTGAGAAATTGATGGTGGCACAATCCATTGACCGGAGTTCTGTTATTCATTCGGAAGGAACAAATGTTGCAGCAGTGAAATTGTCAGCACCCTATTTTCCTGGGAATAGTGAAGGACCACTTAAGAAATATCAAAATGGCTCTACAACTTCGGAACATAATAAACACTCACTTTTAATGCAAACAATAGAGTCTGAATCAGGGACTCAATTGATGAAGTTTGATGCAGATCCAGATATGCAGGATCCTTGTTACCTTCAAGAAACAGAAATCGGTTTTGATGTGAAGGGTGCATCTTTGTTTCCTTCAGAGGTGGAACAGGGCATCTGGCCTGAAAACTATCTCATGTCTGACCATGCTCTACTAAGTGCTGTTTTTTCACCGGTAAAGATACCCCCGTGTGACAAAGCGAACCTTTGA___CCAGAGTGTGGTTCTCCAAAAATGGGTTTTGTAACTGTAGAAAAAGGAAAAATGAATTTATCATGACATACTATGATAACACATTGTTCATCAAAATCCCAGAATTGTAGTTCTCCAAAATACAGTGATTAATCATCAGCAAGCAGTAGTCTTGGTGTCTGAAGTTTACAACACTTCATTACTTGACGAAGTTATATATCTAGTCTCGACTTCATTCTTTTCTGAGATCAGAATGGTTTTAGTCAAAGTTTATATCTGGAAATTTTGCAATCAAAAGATGTACCATTGCCAAGTTTCTCACCAAAAATCAACATATGTAATCGTAGAACACATCAAATCTCTGTAATTTTAGGTCTCTCAAATCACAACACTGAATTTTTATATGTTACGA

Fln Alignment:

Contig3379___KNRRHMALCFNNGDRHDKLAENSVSCTTFNILAPIYKRINGED--SRESQFRDYWISRNKSILDMLLLERSSVICLQEFWLRNEELVEMYEQKFHDGGYEIYKLGRTNNRGDGLFTAVRKDHFKVVNQRELLFHDFGDRVAQLLHLRSVVPSLQSETGGVPYEALVVNTHLLFPHNSDFCLVRLRQVYKILEYLEAFKADYKLPPIPVILCGDWNGSKRGHVYKFLRSQGFVSSYDAAHNYSDNDKDAHKWISHRNHRGNICGVDFIWXXXXXXXXXXXRTSWMEAVFGIIKSKLKEAGFNDLDAFCFFKSAKRFEDYLTLKDFXXXXXXXXLTEQCSEGLTTEEIEDLMVAADLDGNGIIDSWEFEKLMVAQSIDRSSVIHSEGTNVAAVKLSAPYFPGNSEGPLKKYQNGSTTSEHNKHSLLMQTIESESGTQLMKFDADPDMQDPCYLQETEIGFDVKGASLFPSEVEQGIWPENYLMSDHALLSAVFSPVKI
O81916______________RNNKPLSVMRLNSNLASSMVESNISCTTFNILAPIYKRVDQKNHSTRESDFRTLWLARNQRILDLLLHQRSSVICLQEVWVGNEELVNMYHHQLSSSGYTIYQLARTNSRGDGLLTAIHKDHFKVVNYRELLFNDFGDRVAQLLHVKTVIPFPLNGKQDVQQEVIIVNTHLLFPHDSSLSIVRLHQVYKILEYLEAYQKENKLNHMPIILCGDWNGSKRGHVYKFLRSQGFISSYDDAHQYTDS--DAHRWVSHRNHRGNICGVDFIWLCNPSDSRKPLRTSWVEAVFSIIKYQLHKASIAEDDAFTFL-GAKNHSDSLTYSDFCLALQKVNLT-GIPHGLSFEETKELWVRADLDGNGVFDYEELKKIWNMTMVNQ---------------------PGNCK------------------ESVMESKKEEG-------------------EDEAIGLKVNKAILFPQEAEKGLWPENYNISDHACLTVQFSPVKM

SNPs (tot: 23)

Position	A %	C %	G %	T %	Probability
1819	10		90		0.90298
1820		10		90	0.90298
1821	10			90	0.90298
1822			90	10	0.90298
1856		12	87		0.992554
1883	12	87			0.992554
1966	20	80			0.995493
2005	20	80			0.995493
2006		80	20		0.995493
2008	20			80	0.995493
2010	20			80	0.995493
2011		80	20		0.995493
2012		20		80	0.995493
2014	20		80		0.995493
2016			20	80	0.995493
2017	80		20		0.995493
2018		80		20	0.995493
2021		20		80	0.995493
2023	20	80			0.995493
2025			20	80	0.995493
2055	20			80	0.995493
2056		80		20	0.995493
2059			80	20	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain