SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene3344

Length: 1329 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene3344

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	tRNA(Ile)-lysidine synthase, chloroplastic n=1 Tax=Zygnema circumcarinatum RepID=TILS_ZYGCR	-	-	1.0e-11	38%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	80%
Blast2go	hypothetical chloroplast rf62	-	-	0.0	62%

Source	GOs	Term	Type	Identity
Blast2go	tRNA modification	GO:0006400	Biological Process	62%
Blast2go	translation	GO:0006412	Biological Process	62%
Blast2go	ligase activity, forming carbon-nitrogen bonds	GO:0016879	Molecular Function	62%
Blast2go	ATP binding	GO:0005524	Molecular Function	62%
Blast2go	cytoplasm	GO:0005737	Cellular Component	62%

Source	InterPros	Term	Type	Identity
Sma3	tRNA(Ile)-lysidine/2-thiocytidine synthase	IPR011063	-	-
Sma3	Lysidine-tRNA(Ile) synthetase	IPR012094	-	-
Sma3	Lysidine-tRNA(Ile) synthetase, N-terminal	IPR012795	-	-
Sma3	Rossmann-like alpha/beta/alpha sandwich fold	IPR014729	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LL25

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 236 aas, putative chimeric sequence, your sequence is shorter than subject: 175 - 410

Fln protein: MPSFKPQHHHYHRVVTLAVTQSPLLFTPKILLSYPKPHPIHKISALAGFSRELKSNSRANTNIRDHDLAQILVKRLARNLSDRHLLQAGERILVAVSGGQDSICLLWMLSQLQQSWEWKLGVVHCDHQWNPQSSAAQASHVAQVAREMGHNFYQSLPTVDAFGETRARAWRYRL*

Protein Length: 176

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTCTGTGACATGCAAACGCTTGGCCTGCCATTTCACC_-_ATGCCTTCCTTCAAACCCCAACACCACCATTATCACCGCGTCGTGACCTTGGCTGTCACACAATCACCGCTTCTGTTTACACCCAAAATCCTCTTGTCATACCCCAAACCTCATCCTATTCACAAAATTTCAGCACTTGCAGGGTTTTCTCGGGAGCTCAAATCAAATTCTCGAGCAAATACAAACATAAGAGACCACGACCTCGCTCAAATCTTGGTGAAGCGACTGGCCCGAAATTTATCGGACAGACACCTATTGCAGGCGGGGGAGAGAATCCTGGTGGCCGTTTCAGGTGGGCAGGACTCCATATGCCTTCTGTGGATGCTCTCTCAGTTGCAGCAAAGCTGGGAATGGAAGTTGGGTGTGGTTCACTGCGACCATCAATGGAATCCTCAATCATCAGCAGCACAGGCTTCCCACGTTGCCCAAGTCGCCAGGGAAATGGGCCACAATTTCTATCAGAGCTTGCCCACCGTGGACGCCTTCGGCGAAACCAGAGCTCGGGCATGGAGGTATAGGTTGTGA___TGTCCTGGTTCTGAGTATGTAGTGCTGGAGTGGTTCCAGGATCCCAGTGGGTGAAGAATGAGTCAAGAGCAGTGTTAAGCAGGTGGATTGTTCTCTCAGTGTGACTGGCAGTTTGTTGGATGGTTTCTAATTCACATGAGATTCTAATATCTCAATCAAAAATCCTAATTTGAATTGCTACTTTTGTGGTCATTCTTGTCATCTTTGACTTTCCAAAACCTTAATCCACCTTACCATCTTTGTGAACTCCAAACCTTACTTTGATTTTCTATTCTTAGCCAAAAAAGTCAAACTGACCCATGCTAATTTTTTCAAGTTGCATACTAATTCGACCTCTCTTTTCCTGTTTAGTTAAACGCATAATTGACTTCATCCCTTTGGCAGGTTGGATTTCCTAAAACCTTCTGCTGTCCAGGTTTAGCATCCAGGCAAATTTGCGTCTTGGAAGATAGATTTGTGGGTTATTGTGGGGTTGTATGTGATGCAACTTGTCCATCTGAATGATCATCTGACTTCCAAACAATATAGTGATTGCATATCTGGTTGTCAGCACATTTACTATAGCATTTTCAGCATGCGACTAGGTGGTTAAATCAACCACACAGCATTTGGTGGATCTATTTTAATGAACAGACTTGGAGATAGTTATCCCGCAAAGAATTAAAGCTTACTGGAATATTATGTTCTATAATTCACACAGCATTTGGTGTATGTATTTTAATGAACAGACTTGGAGATAGT

Fln Alignment:

Contig3344___PQHHHYHRVVTLAVTQSPLLFTPKILLSYPKPHPIHKISALAGFSRELKSNSRANTNIRDHDLAQILVKRLARNLSDRHLLQAGERILVAVSGGQDSICXXXXXXXXXXXXEWKLGVVHCDHQWNPXXXXXXXXXXXXXXREMGHNFYQSLPTVDAFGETRARAWRY
B8LL25______________PLHHVFPQTPTPPFR--PLLVTPKVLLSNPKPHPLHKISAASGFSQEPKSNSSANANRRDHDIPQILVKRLARNLSDRHLVQAGERILVAVSGGQDSICLLWMLSQLQQSWEWKVGVVHCDHQWNPQSSAAQASHVAHVAREMGYDFYQSLPTMDAFGETRARTWRY

SNPs (tot: 36)

Position	A %	C %	G %	T %	Probability
717		33		66	0.997452
719		66		33	0.997452
720		33		66	0.997452
738	75	25			0.996473
739	75		25		0.996473
742		25		75	0.996473
745		75		25	0.996473
746	75		25		0.996473
747	75		25		0.996473
748		25		75	0.996473
749		75		25	0.996473
750	50	25	25		0.995533
751	75			25	0.996473
752	75	25			0.996473
753	80	20			0.995493
754	60	20	20		0.99255
757		80	20		0.995493
760	80	20			0.995493
761		20		80	0.995493
765	80		20		0.995493
766	80	20			0.995493
769	20		80		0.995493
774	20			80	0.995493
883	85	14			0.993533
884	85			14	0.993533
885		85	14		0.993533
887		14	85		0.993533
890		85		14	0.993533
950			87	12	0.992554
1008			88	11	0.923564
1206		28		71	1.0
1246	50		50		1.0
1266	16		83		0.994512
1267		16	83		0.994512
1269	83			16	0.994512
1271	60		40		1.0

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain