SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene3258

Length: 2700 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene3258
ATGGGGGAGAAGCGCGGGTGGGAATTAGGATAGTAGATTTATAATGGTGGTCACGCCAGTTCCTGCAGCAGCAAGCAAGCACACAATGATGGCATTTATGGTTTCCAGCTGCCTTTAGCATCATCTGCTACTGAAACTTCCATGAATGTTCAAAATAGTTTGAAAATTCACGAGATTTGTGTTCACTATAGGCAGTATGTAAGGGATTTCAGATTGGACTTTGTCCAGAAAACGGTATTGGGGCATGGAGTGGAGTAGTTGGAGAATTTTGGGTGTTGAAGGAGTCTAGAGGTATTCATAGAACTGTGGGCGTTAATTTTGGAGGATAGAAATTTTCAGAGAATCGTGGCTGTTCATCTGTATTGACTATTTCGAAAGAAGAATGAAGATGGAATAGCAGTGGAATTTCTCCAAGATATTTTTTATGATGTTTGTGTGTATGTTTCCCAGGGCAGAGTCGACTGAATGTTTGCTGTTATTGAGTGTGATTCAAGCCTAGTTGCCTACTTGCATACTTTTCAAAGATTTGTACTCTGTTTTGATAATATGAGCAGCTATTTCAAATGCAAGGCCTGCTTTATTTGGATATTTGAGCAGTGGTCACTCTCCCAAACTGGGTATATTATACATAGCCAAGACAAGAAAGAGGAGTAGATCAGGAACGAGAAATAGGTCTCTGGTTAGGTTCCTGGCAACATAGCCAGCATTTGTAAAGGAGAGAGCGTCGTGACCTTGGCTGTCACTCATCTTTTCAGCACATAGATGAGTTTATTAGCAAGTTGCCAATCATGGAGCATGGGAATTTGCATCTACTATGGTTGATTGTGGCAACTGTGTGCATATTTCTCCAGATTGGAGCGAGTGCGATCGAGAATCCAGAAGTGTCTGCTCTCCAGGCTATTCAAAGAGTTTATCGTTTAAACTTAGGCTGGGAAAATGATCCATGTTCTCCAGTTCCATGGAAGGGTCTTCAATGTGATGAAGCAAACGACGTTGTCTCTTTGGACCTCTCAAACATGAATCTACAGGGACCAATATCTGTTGCATTCGGGGACCTTCTAGATCTTCAAAACCTGGATTTGCATAATAACTCACTAACTGGACAAATTGAGAACTTTAATGCTCTCTCTCTACTTCAAACACTGAATCTGAGCTTCAACAAACTAAATGCACGCATACCTGACCTAAGTTCCTTAACTAGCTTGCAGAAACTGGATTTGCAAAACAACAGTCTTCAGGGGACAGTTCCGGAGAGCCTGGGAAGATTGCCAAGTCTTGACTTATTAAATTTGGAAAACAATCAACTTTATGGGAACCTGCCCATCTCCCTGAACAAGACTACACTGGATTTAAGAACATCGGGCAATCTATGTCTGACATTCCCTGGTACAAGATGTTCTCCTGCCCCTCCTCCACTTCAGACCCCGGAAGTGACAGAACCGAACATATCCAAAAGAAAAGCCACATCAAGGCTATTATTGGAGGCCTTGTAGGTGGAATAGCATTTGTTGTAATCGTGTGTTCAGCTCTACTAATATGGTACAAAAGAAAACACAAACAGGAGGATGGAAGGTCCATGTCAAAAACAGACATGAGAAATTGGAATGCAGCAAGACGGTTTACCTTCAAGGAAATTAAACTGGCAACAAATAGTTTTAAGGAGACAATAGGCCGTGGAAGCTTTGGATCTGTTTATCTTGGAAAGCTTCAAGAGGGAAACCGTGTTGCTGTGAAAGTACGCTTTGATCCATCACAACTAGCAGCTGACTCTTTTATTAATGAGGTATTCTTGCTCTCCCAAGTTCACCATCAAAACCTTGTTACATTGGAAGGATTTTGTCAAGAGCCTAAGCATCAAATTTTGGTATATGAATATTTGCCCGAAGGAGCTCTGACAGATCATCTTTATGGTGCTAAAGGCAAAAGGAACCCATTGGACTGGAAGAAACGATTAAAAATTGCCGTGGACGCTGCAGCAGGAATCGAGTATCTTCACAATGGAAGTAATACCAAGATCATCCACCGTGATGTAAAGTGCAGCAACATTCTTCTGGATTCAGCCATGAATGCCAAAGTTTGTGACTTCGGCCTTTCTAAGCAAATTGTTCAAGCAGATGCAACTCATGTGACAACAGTTGTCAAGGGAACTGCCGGGTATCTTGACCCTGAGTATTATTCAACACAACAGCTGACTGAAAAAAGCGATGTCTATAGTTTTGGGGTGGTGCTTCTAGAGTTGATATGTGGACGTGAACCTTTGACTCACACAGGTTCCCCAGATACCTATAATCTGGTCTTATGGGCGAAGCCCTACCTACAGGCTGGAGACTACAATGTTGTAGACAAGAACCTTCAGAACAAGTACAGCACAGAAAGCTTAAAGAGAGTTGCTTCCATTGCCTCGAGGTGTGTGGAGAGAGATGCTTCACAGAGGCCAACTATGGCAGAAGTCCTGTCCGAGCTCAAGGAAGCCTTGGCTATTGAAGAAACTGAAAATCCAACGACTGAAGAGATCTGAGCAATGACTATTCATACTAGAAATGGCCCGCCACAACTTACATTATTTTCTCAGTTTTCACCACATCATGGCATGGCAATCTTTATTTTCTTTCTTCCATTTTTCTATTGCAAAGAAACTAATTAAGTTATATGGCCATCTTCTGAGATGCCCGTAAAATATGTTCTTAGGGTGATAGCCTA

Ace file of the UniGene sp_v2.0_unigene3258 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	putative LRR receptor-like serine/threonine-protein kinase [Arabidopsis thaliana] sp\|C0LGV0.1\|Y5487_ARATH RecName: Full=Probable LRR receptor-like serine/threonine-protein kinase At5g48740; Flags: Precursor gb\|ACN59385.1\| leucine-rich repeat receptor-like	-	-	64%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	45%
Blast2go	probable lrr receptor-like serine threonine-protein kinase at1g67720-like	-	-	70%

Source	ECs	Term	Type	e value	Identity
Blast2go	Transferases, Transferring phosphorous-containing groups, Protein-serine/threonine kinases.	EC:2.7.11.-	-	0.0	70%

Source	GOs	Term	Type	Identity
Blast2go	protein phosphorylation	GO:0006468	Biological Process	70%
Blast2go	signal transducer activity	GO:0004871	Molecular Function	70%
Blast2go	ATP binding	GO:0005524	Molecular Function	70%
Blast2go	protein serine/threonine kinase activity	GO:0004674	Molecular Function	70%
Blast2go	endomembrane system	GO:0012505	Cellular Component	70%

Source	InterPros	Term	Type	Identity
Sma3	Histone-like bacterial DNA-binding protein	IPR000119	-	-
Sma3	Oxidoreductase, molybdopterin-binding domain	IPR000572	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Beta-lactamase, class-A/D	IPR000871	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Actinin-type, actin-binding, conserved site	IPR001589	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	Short-chain dehydrogenase/reductase SDR	IPR002198	-	-
Sma3	Glucose/ribitol dehydrogenase	IPR002347	-	-
Sma3	Leucine-rich repeat, typical subtype	IPR003591	-	-
Sma3	Phosphopantetheine attachment site	IPR006162	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Leucine-rich repeat-containing N-terminal, type 2	IPR013210	-	-
Sma3	SKG6/AXL2 alpha-helix transmembrane domain	IPR014805	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5AAA3

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 73 aas, putative chimeric sequence, your sequence is shorter than subject: 383 - 458

Fln protein: MSDIPWYKMFSCPSSTSDPGSDRTEHIQKKSHIKAIIGGLVGGIAFVVIVCSALLIWYKRKHKQEDGRSMSKTDMRNWNAARRFTFKEIKLATNSFKETIGRGSFGSVYLGKLQEGNRVAVKVRFDPSQLAADSFINEVFLLSQVHHQNLVTLEGFCQEPKHQILVYEYLPEGALTDHLYGAKGKRNPLDWKKRLKIAVDAAAGIEYLHNGSNTKIIHRDVKCSNILLDSAMNAKVCDFGLSKQIVQADATHVTTVVKGTAGYLDPEYYSTQQLTEKSDVYSFGVVLLELICGREPLTHTGSPDTYNLVLWAKPYLQAGDYNVVDKNLQNKYSTESLKRVASIASRCVERDASQRPTMAEVLSELKEALAIEETENPTTEEI*

Protein Length: 384

Fln nts: ATGGGGGAGAAGCGCGGGTGGGAATTAGGATAGTAGATTTATAATGGTGGTCACGCCAGTTCCTGCAGCAGCAAGCAAGCACACAATGATGGCATTTATGGTTTCCAGCTGCCTTTAGCATCATCTGCTACTGAAACTTCCATGAATGTTCAAAATAGTTTGAAAATTCACGAGATTTGTGTTCACTATAGGCAGTATGTAAGGGATTTCAGATTGGACTTTGTCCAGAAAACGGTATTGGGGCATGGAGTGGAGTAGTTGGAGAATTTTGGGTGTTGAAGGAGTCTAGAGGTATTCATAGAACTGTGGGCGTTAATTTTGGAGGATAGAAATTTTCAGAGAATCGTGGCTGTTCATCTGTATTGACTATTTCGAAAGAAGAATGAAGATGGAATAGCAGTGGAATTTCTCCAAGATATTTTTTATGATGTTTGTGTGTATGTTTCCCAGGGCAGAGTCGACTGAATGTTTGCTGTTATTGAGTGTGATTCAAGCCTAGTTGCCTACTTGCATACTTTTCAAAGATTTGTACTCTGTTTTGATAATATGAGCAGCTATTTCAAATGCAAGGCCTGCTTTATTTGGATATTTGAGCAGTGGTCACTCTCCCAAACTGGGTATATTATACATAGCCAAGACAAGAAAGAGGAGTAGATCAGGAACGAGAAATAGGTCTCTGGTTAGGTTCCTGGCAACATAGCCAGCATTTGTAAAGGAGAGAGCGTCGTGACCTTGGCTGTCACTCATCTTTTCAGCACATAGATGAGTTTATTAGCAAGTTGCCAATCATGGAGCATGGGAATTTGCATCTACTATGGTTGATTGTGGCAACTGTGTGCATATTTCTCCAGATTGGAGCGAGTGCGATCGAGAATCCAGAAGTGTCTGCTCTCCAGGCTATTCAAAGAGTTTATCGTTTAAACTTAGGCTGGGAAAATGATCCATGTTCTCCAGTTCCATGGAAGGGTCTTCAATGTGATGAAGCAAACGACGTTGTCTCTTTGGACCTCTCAAACATGAATCTACAGGGACCAATATCTGTTGCATTCGGGGACCTTCTAGATCTTCAAAACCTGGATTTGCATAATAACTCACTAACTGGACAAATTGAGAACTTTAATGCTCTCTCTCTACTTCAAACACTGAATCTGAGCTTCAACAAACTAAATGCACGCATACCTGACCTAAGTTCCTTAACTAGCTTGCAGAAACTGGATTTGCAAAACAACAGTCTTCAGGGGACAGTTCCGGAGAGCCTGGGAAGATTGCCAAGTCTTGACTTATTAAATTTGGAAAACAATCAACTTTATGGGAACCTGCCCATCTCCCTGAACAAGACTACACTGGATTTAAGAACATCGGGCAATCT_-_ATGTCTGACATTCCCTGGTACAAGATGTTCTCCTGCCCCTCCTCCACTTCAGACCCCGGAAGTGACAGAACCGAACATATCCAAAAGAAAAGCCACATCAAGGCTATTATTGGAGGCCTTGTAGGTGGAATAGCATTTGTTGTAATCGTGTGTTCAGCTCTACTAATATGGTACAAAAGAAAACACAAACAGGAGGATGGAAGGTCCATGTCAAAAACAGACATGAGAAATTGGAATGCAGCAAGACGGTTTACCTTCAAGGAAATTAAACTGGCAACAAATAGTTTTAAGGAGACAATAGGCCGTGGAAGCTTTGGATCTGTTTATCTTGGAAAGCTTCAAGAGGGAAACCGTGTTGCTGTGAAAGTACGCTTTGATCCATCACAACTAGCAGCTGACTCTTTTATTAATGAGGTATTCTTGCTCTCCCAAGTTCACCATCAAAACCTTGTTACATTGGAAGGATTTTGTCAAGAGCCTAAGCATCAAATTTTGGTATATGAATATTTGCCCGAAGGAGCTCTGACAGATCATCTTTATGGTGCTAAAGGCAAAAGGAACCCATTGGACTGGAAGAAACGATTAAAAATTGCCGTGGACGCTGCAGCAGGAATCGAGTATCTTCACAATGGAAGTAATACCAAGATCATCCACCGTGATGTAAAGTGCAGCAACATTCTTCTGGATTCAGCCATGAATGCCAAAGTTTGTGACTTCGGCCTTTCTAAGCAAATTGTTCAAGCAGATGCAACTCATGTGACAACAGTTGTCAAGGGAACTGCCGGGTATCTTGACCCTGAGTATTATTCAACACAACAGCTGACTGAAAAAAGCGATGTCTATAGTTTTGGGGTGGTGCTTCTAGAGTTGATATGTGGACGTGAACCTTTGACTCACACAGGTTCCCCAGATACCTATAATCTGGTCTTATGGGCGAAGCCCTACCTACAGGCTGGAGACTACAATGTTGTAGACAAGAACCTTCAGAACAAGTACAGCACAGAAAGCTTAAAGAGAGTTGCTTCCATTGCCTCGAGGTGTGTGGAGAGAGATGCTTCACAGAGGCCAACTATGGCAGAAGTCCTGTCCGAGCTCAAGGAAGCCTTGGCTATTGAAGAAACTGAAAATCCAACGACTGAAGAGATCTGA___GCAATGACTATTCATACTAGAAATGGCCCGCCACAACTTACATTATTTTCTCAGTTTTCACCACATCATGGCATGGCAATCTTTATTTTCTTTCTTCCATTTTTCTATTGCAAAGAAACTAATTAAGTTATATGGCCATCTTCTGAGATGCCCGTAAAATATGTTCTTAGGGTGATAGCCTA

Fln Alignment:

Contig3258___DGRSMSKTDMRNWNAARRFTFKEIKLATNSFKET--IGRGSFGSVYLGKLQE-GNRVAVKVRFDPSQLAADSFINEVFLLSQVHHQNLVTLEGFCQEPKHQILVYEYLPEGALTDHLYGAKGKRNPLDWKKRLKIAVDAAAGIEYLHNGSNTKIIHRDVKCSNILLDSAMNAKVCDFGLSKQIVQADATHVTTVVKGTAGYLDPEYYSTQQLTEKSDVYSFGVVLLELICGREPLTHTGSPDTYNLVLWAKPYL--QAGDYNVVDKNLQNKYSTESLKRVASIASRCVERDASQRPTMAEVLSEL
D5AAA3______________DSRKEVSRDGSQHIAAQTFTFRELAAATKNFRPECLLGEGGFGRVYRGRLESTGQAVAVKQLDRNGVQGNREFLVEVLMLSLLHHDNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLHDLPPDKEPLDWKTRMKIAAGAAKGLEYLHDKANPPVIYRDLKCSNILLDEGYHSKLSDFGLAKLGPVGDKTHVSTRVMGTYGYCAPEYAMTGQLTIKSDVYSFGVVLLELITGRKAIDNSRSAGENNLVAWARPLFKDRRKFSQMADPLLQCRYPMRGLYQALAVAAMCVQEQATMRPLIADVVTAL

SNPs (tot: 29)

Position	A %	C %	G %	T %	Probability
530	75	25			0.996473
1640		75	25		0.996473
1641	25			75	0.996473
1642	75		25		0.996473
1646	75	25			0.996473
1647	25		75		0.996473
1652	25	75			0.996473
1653	25	75			0.996473
1655	75		25		0.996473
1656	75			25	0.996473
1657		25		75	0.996473
1659	25	75			0.996473
1660	75			25	0.996473
1779	75		25		0.996473
1802	75	25			0.996473
1803	75	25			0.996473
1804		25		75	0.996473
1806			75	25	0.996473
1959			75	25	0.996473
1960	75		25		0.996473
1963		25	75		0.996473
1970		75		25	0.996473
1971	75	25			0.996473
1972	75	25			0.996473
1977		75	25		0.996473
1978	75			25	0.996473
1981		25	75		0.996473
1982	75	25			0.996473
2434	75	25			0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain