SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene3012

Length: 2356 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene3012
ATGGGGACGACATTTTATAGCCAGAGCGCCTCTTCCTTTTTTTGGATATTCCTAGATTTCGCGCGGAAAGAGGTGGGGCTATTTTCGACGGTTGCAAATTCATAAGTAAACGCGCCAACGAAAAATGTAGAGAAACCAGAAACCCTAACCAGGCGTGATTCGAATTGAGCATTGAATAGTAGCAAGCCTGAAACTTGCAAATCAACTCATTCCCTCTATGTTTGGATAAATGCGAATCCTCCGGTTGAAGAGCTGGTTCAAGTTCTTACAGGTGTTCTGACCTAGTAGCTAAGTATCCATCTGAGTAGCCCAGGCAAGCGGATGAGCGACCAGCGTAGTATCCAAGCGGATTGTCGCACCCTGTAAGCAAGGAGATCGACCGACCTGTGCAGCAGTTATCGGTAGCATATGTATATAATAGGGGCACGGAACTAACACTGTTGTTCCTCAGAGTGAGAAAGTGCCGTCTTTCTAAGCAAATAACTCCCGGGCAAGTTATTATTAATAGGCCAATATTTGCCATATCAACATATATACAGCAGTACGGAAAACACTAGAGATAGATAAAAGCTGCCGCTTTTGAATCAGTCCTAGGATTTGAGTACAAGCCGATAGTGAATTTAGTTATGGCGGCCGTTGAAGAAGAAGAGGAGGTTCATACCTTTGCCAGTTTGGGCGTATGTGAGCAGCTAACCGAAGCCTGCGATGCTCTGGGATGGAAGAAACCATCTCCAATTCAAGTCCAATCCATTCCCCTGGCCTTGGAAGGTAGGGATTTGATCGGGTTGGCTCAGACAGGTTCAGGTAAAACAGCAGCATTTGCTATACCAATTCTGCAGGCACTTCTTGAGAAACCGCAGCCATTGTTTGCCTGTGTTCTTTCTCCAACCAGGGAACTGGCAATTCAGATTGCAGAGCAGTTTGAAGCACTGGGTTCTGGCATTGGTGTAAAGTGTGTCGTGGTGGTTGGCGGTGTTGACATGATGGCACAAGCAATAGCTTTGGCCAAAAGGCCTCATATTTTGGTAGGAACTCCAGGACGTCTTTTAGATCATTTATCAAATACAAAGGGCTTCAATCTACGAGCATTGAAGTATTTGGTGTTAGATGAAGCAGACAGGTTGTTGAACATGGATTTTGAACAAGAAATTGATGAAATCCTTAAGGTTATTCCTAGGGAGAGAAAGACATATTTATTCTCGGCAACAATGACAAATAAGGTGGCCAAGCTTCAGAGAGCATGTTTGAAGAATCCTGCAAAGGTTGAGGTTTCTTCAAAGTACTCAACTGTGGATACATTGAAACAGCATTATCTTTTCGTTCCTGCTAAGCATAAGGATTGCTATCTTGTGTTTCTTTTAAATGAGATGTCTGGAAGTACTACAATGGTTTTCACTCGCACATGTGAGTCTACAAGGCGTATTGCTTTGATTCTTCGAAATCTTGGACTTGAGGCCATTCCTATAAGTGGGCAGATGAGTCAGCCAAAAAGGATAGGAGCTCTAAACAAGTTCAAAGCTGGAGAGCGTAACATCCTTATCTGCACTGATGTTGCTAGCAGAGGGCTAGATATTCCTTCTGTAGATATGGTTATCAACTATGATATACCTACAAACTCAAAGGATTATATTCACAGAGTGGGAAGAACAGCACGTGCTGGTCGGTCAGGTCTCGCAGTGTCTTTAGTCACTCAATATGATGTTGAGCTATACCAGCGTATAGAACAATTGATAGGTAAGAAACTCACTACATTTCCAGCCGAGCAAGAAGAGGTTTTACAACTATATGAACGAGTTAATGAGGCCCAAAGAATTTCTGCCACGAAAATTCGTGAAAAGGATGGCAACAAGAAGCATAAGAAAAGGAGGGGTGAGGTTGGGGATGATGGTGATGATGGTGATGAAGGATTTTCAGGTCCCAAAAGCAATAAAAATTTGAAGTTAAAGCGTCGGAGATGACAATGGTATCTCCATAGCATACAGTTGTGTATTTTCAAGGTTAGAGATGATGCTATAGAAGCTGATCGGGCATGCATGTCAGATCCGTGGAATGTTTCAGAGCTAATATATTATTTTGAAGAAACTGCAATTGCTGTTTCATTTGAAGTTCTATAGAACAACATCAAGAATATGATTGTAAATTTTAGCTTGGAAGTTTCATTTCCTGACTTTTCAGTTCGCTGATTGTATGGGTAGCTCTTCCCCATGAGTTGCTTTGCACAATTCTACTGCTTCATATTCTTGAGTTGTTATAGTTTGTATCATGTATCATGAATGTAACATTTTTTTATGAAGTGACAATTTTGTATGTCTACATAATATACAATAAGAATGAAATCAGAGCAATCTTTATAAAG

Ace file of the UniGene sp_v2.0_unigene3012 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	pfam00270, DEAD, DEAD/DEAH box helicase	-	-	50%
FL-Next	sp=DEAD-box ATP-dependent RNA helicase 10; Arabidopsis thaliana (Mouse-ear cress).	-	-	69%
Blast2go	protein	-	-	86%

Source	GOs	Term	Type	Identity
Blast2go	ATP-dependent helicase activity	GO:0008026	Molecular Function	86%
Blast2go	RNA binding	GO:0003723	Molecular Function	86%
Blast2go	ATP binding	GO:0005524	Molecular Function	86%
Blast2go	nucleolus	GO:0005730	Cellular Component	86%

Source	InterPros	Term	Type	Identity
Sma3	Helicase, superfamily 1/2, ATP-binding domain	IPR014001	-	-
Sma3	Helicase, C-terminal	IPR001650	-	-
Sma3	DNA/RNA helicase, DEAD/DEAH box type, N-terminal	IPR011545	-	-
Sma3		IPR014021	-	-
Sma3	RNA helicase, ATP-dependent, DEAD-box, conserved site	IPR000629	-	-
Sma3	RNA helicase, DEAD-box type, Q motif	IPR014014	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: sp_plants

Fln subject: Q8GY84

Fln msg:

Fln protein: MAAVEEEEEVHTFASLGVCEQLTEACDALGWKKPSPIQVQSIPLALEGRDLIGLAQTGSGKTAAFAIPILQALLEKPQPLFACVLSPTRELAIQIAEQFEALGSGIGVKCVVVVGGVDMMAQAIALAKRPHILVGTPGRLLDHLSNTKGFNLRALKYLVLDEADRLLNMDFEQEIDEILKVIPRERKTYLFSATMTNKVAKLQRACLKNPAKVEVSSKYSTVDTLKQHYLFVPAKHKDCYLVFLLNEMSGSTTMVFTRTCESTRRIALILRNLGLEAIPISGQMSQPKRIGALNKFKAGERNILICTDVASRGLDIPSVDMVINYDIPTNSKDYIHRVGRTARAGRSGLAVSLVTQYDVELYQRIEQLIGKKLTTFPAEQEEVLQLYERVNEAQRISATKIREKDGNKKHKKRRGEVGDDGDDGDEGFSGPKSNKNLKLKRRR*

Protein Length: 445

Fln nts: ATGGGGACGACATTTTATAGCCAGAGCGCCTCTTCCTTTTTTTGGATATTCCTAGATTTCGCGCGGAAAGAGGTGGGGCTATTTTCGACGGTTGCAAATTCATAAGTAAACGCGCCAACGAAAAATGTAGAGAAACCAGAAACCCTAACCAGGCGTGATTCGAATTGAGCATTGAATAGTAGCAAGCCTGAAACTTGCAAATCAACTCATTCCCTCTATGTTTGGATAAATGCGAATCCTCCGGTTGAAGAGCTGGTTCAAGTTCTTACAGGTGTTCTGACCTAGTAGCTAAGTATCCATCTGAGTAGCCCAGGCAAGCGGATGAGCGACCAGCGTAGTATCCAAGCGGATTGTCGCACCCTGTAAGCAAGGAGATCGACCGACCTGTGCAGCAGTTATCGGTAGCATATGTATATAATAGGGGCACGGAACTAACACTGTTGTTCCTCAGAGTGAGAAAGTGCCGTCTTTCTAAGCAAATAACTCCCGGGCAAGTTATTATTAATAGGCCAATATTTGCCATATCAACATATATACAGCAGTACGGAAAACACTAGAGATAGATAAAAGCTGCCGCTTTTGAATCAGTCCTAGGATTTGAGTACAAGCCGATAGTGAATTTAGTT_-_ATGGCGGCCGTTGAAGAAGAAGAGGAGGTTCATACCTTTGCCAGTTTGGGCGTATGTGAGCAGCTAACCGAAGCCTGCGATGCTCTGGGATGGAAGAAACCATCTCCAATTCAAGTCCAATCCATTCCCCTGGCCTTGGAAGGTAGGGATTTGATCGGGTTGGCTCAGACAGGTTCAGGTAAAACAGCAGCATTTGCTATACCAATTCTGCAGGCACTTCTTGAGAAACCGCAGCCATTGTTTGCCTGTGTTCTTTCTCCAACCAGGGAACTGGCAATTCAGATTGCAGAGCAGTTTGAAGCACTGGGTTCTGGCATTGGTGTAAAGTGTGTCGTGGTGGTTGGCGGTGTTGACATGATGGCACAAGCAATAGCTTTGGCCAAAAGGCCTCATATTTTGGTAGGAACTCCAGGACGTCTTTTAGATCATTTATCAAATACAAAGGGCTTCAATCTACGAGCATTGAAGTATTTGGTGTTAGATGAAGCAGACAGGTTGTTGAACATGGATTTTGAACAAGAAATTGATGAAATCCTTAAGGTTATTCCTAGGGAGAGAAAGACATATTTATTCTCGGCAACAATGACAAATAAGGTGGCCAAGCTTCAGAGAGCATGTTTGAAGAATCCTGCAAAGGTTGAGGTTTCTTCAAAGTACTCAACTGTGGATACATTGAAACAGCATTATCTTTTCGTTCCTGCTAAGCATAAGGATTGCTATCTTGTGTTTCTTTTAAATGAGATGTCTGGAAGTACTACAATGGTTTTCACTCGCACATGTGAGTCTACAAGGCGTATTGCTTTGATTCTTCGAAATCTTGGACTTGAGGCCATTCCTATAAGTGGGCAGATGAGTCAGCCAAAAAGGATAGGAGCTCTAAACAAGTTCAAAGCTGGAGAGCGTAACATCCTTATCTGCACTGATGTTGCTAGCAGAGGGCTAGATATTCCTTCTGTAGATATGGTTATCAACTATGATATACCTACAAACTCAAAGGATTATATTCACAGAGTGGGAAGAACAGCACGTGCTGGTCGGTCAGGTCTCGCAGTGTCTTTAGTCACTCAATATGATGTTGAGCTATACCAGCGTATAGAACAATTGATAGGTAAGAAACTCACTACATTTCCAGCCGAGCAAGAAGAGGTTTTACAACTATATGAACGAGTTAATGAGGCCCAAAGAATTTCTGCCACGAAAATTCGTGAAAAGGATGGCAACAAGAAGCATAAGAAAAGGAGGGGTGAGGTTGGGGATGATGGTGATGATGGTGATGAAGGATTTTCAGGTCCCAAAAGCAATAAAAATTTGAAGTTAAAGCGTCGGAGATGA___CAATGGTATCTCCATAGCATACAGTTGTGTATTTTCAAGGTTAGAGATGATGCTATAGAAGCTGATCGGGCATGCATGTCAGATCCGTGGAATGTTTCAGAGCTAATATATTATTTTGAAGAAACTGCAATTGCTGTTTCATTTGAAGTTCTATAGAACAACATCAAGAATATGATTGTAAATTTTAGCTTGGAAGTTTCATTTCCTGACTTTTCAGTTCGCTGATTGTATGGGTAGCTCTTCCCCATGAGTTGCTTTGCACAATTCTACTGCTTCATATTCTTGAGTTGTTATAGTTTGTATCATGTATCATGAATGTAACATTTTTTTATGAAGTGACAATTTTGTATGTCTACATAATATACAATAAGAATGAAATCAGAGCAATCTTTATAAAG

Fln Alignment:

Contig3012___TFASLGVCEQLTEACDALGWKKPSPIQVQSIPLALEGRDLIGLAQTGSGKTAAFAIPILQALLE-----------KPQP-LFACVLSPTRELAIQIAEQFEALXXXXXXXXXXXXXXVDMMAQAIALAKRPHILVGTPGRLLDHLSNTKGFNLRALKYLVLDEADRLLNMDFEQEIDEILKVIPRERKTYLFSATMTNKVAKLQRACLKNPAKVEVSSKYSTVDTLKQHYLFVPAKHKDCYLVFLLNEMSGSTTMVFTRTCESTRRIALILRNLGLEAIPISGQMSQPKRIGALNKFKAGERNILICTDVASRGLDIPSVDMVINYDIPTNSKDYIHRVGRTARAGRSGLAVSLVTQYDVELYQRIEQLIGKKLTTFPAEQEEVLQLYERVNEAQRISATKIREKDGNKK
Q8GY84______________TFAELGVREELVKACERLGWKNPSKIQAEALPFALEGKDVIGLAQTGSGKTGAFAIPILQALLEYVYDSEPKKGRRPDPAFFACVLSPTRELAIQIAEQFEALGADISLRCAVLVGGIDRMQQTIALGKRPHVIVATPGRLWDHMSDTKGFSLKSLKYLVLDEADRLLNEDFEKSLNQILEEIPLERKTFLFSATMTKKVRKLQRACLRNPVKIEAASKYSTVDTLKQQYRFVAAKYKDCYLVYILSEMPESTSMIFTRTCDGTRFLALVLRSLGFRAIPISGQMTQSKRLGALNKFKAGECNILVCTDVASRGLDIPSVDVVINYDIPTNSKDYIHRVGRTARAGRSGVGISLVNQYELEWYIQIEKLIGKKLPEYPAEEDEVLSLLERVAEAKKLSAMNMKESGGRKR

SNPs (tot: 21)

Position	A %	C %	G %	T %	Probability
1698			66	33	0.997452
1814		75		25	0.996473
1837	75		25		0.996473
1958	80	20			0.995493
1991		20		80	0.995493
2078	88			11	0.923564
2082		88	11		0.923564
2084		11	88		0.923564
2086	88		11		0.923564
2088	88	11			0.923564
2089	11	88			0.923564
2090	11	88			0.923564
2091			11	88	0.923564
2094	88	11			0.923564
2095	88		11		0.923564
2096		11	88		0.923564
2097	88		11		0.923564
2100			11	88	0.923564
2142	80		20		0.995493
2148			80	20	0.995493
2151	80	20			0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain