SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene2921

Length: 1602 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene2921

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	chloroplast Hcf106-1 [Physcomitrella patens]	-	-	9.0e-36	59%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	86%
Blast2go	protein	-	-	0.0	66%

Source	GOs	Term	Type	Identity
Blast2go	protein secretion	GO:0009306	Biological Process	66%
Blast2go	protein import into chloroplast thylakoid membrane	GO:0045038	Biological Process	66%
Blast2go	proton motive force dependent protein transmembrane transporter activity	GO:0009977	Molecular Function	66%
Blast2go	chloroplast thylakoid membrane	GO:0009535	Cellular Component	66%

Source	InterPros	Term	Type	Identity
Sma3	Twin-arginine translocation protein TatA/B/E	IPR003369	-	-
Sma3	Twin-arginine translocation protein TatA/E	IPR006312	-	-
Sma3	Twin-arginine translocation protein TatB-like	IPR003998	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9P230

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 44 aas, putative chimeric sequence, your sequence is shorter than subject: 226 - 275

Fln protein: MATAGPWALSSPCLCSSVFPTIGFRSKHKWVSSGLSSQHLCRATHTQKAHFEPLDRTRIAFFIGWNARTHLGQSFLFNAPNRGFHRQKKGSGVQASLFGVGAPEALVIGVVALLVFGPKGLAEVARTLGKSLRAFQPTIKELQEVSREFKNTLEQEIGLDEFRSTTINPSNSAPPTLQRETLVEQNGPPGRKPYSAEDYLRVSQEEIKALVPEEKPKGSEPEQLE*

Protein Length: 227

Fln nts: CGTCGTGACCTTGGCTGTCATGGGGTGGAGGGATAAATGCGAAGTTCACGTGAGTGGGATTGAACTGGTTGTAAACGAAATTGTAAGCTACTACAGAAATTGGGCTGACGTTCTGTATTATCAGTGGAATTACAAAAAAGTGAACGACATTCCGCGCAAATTTCTGGGATCAAGGAAAGGAGAGGATCGGGT_-_ATGGCTACGGCAGGGCCATGGGCACTGAGTTCACCCTGCTTGTGCTCTTCCGTGTTTCCTACAATCGGTTTTCGCTCTAAACACAAGTGGGTTTCTTCCGGACTGTCCTCTCAACACCTCTGCAGGGCCACGCATACACAAAAAGCTCATTTTGAGCCACTCGACAGAACGAGGATTGCCTTCTTCATAGGTTGGAATGCTCGCACCCATTTGGGTCAGTCATTCCTATTCAACGCACCAAACAGAGGTTTCCACAGGCAGAAAAAGGGCAGTGGTGTGCAGGCCTCATTGTTTGGAGTAGGGGCCCCTGAGGCACTGGTGATTGGAGTTGTAGCTCTGCTCGTCTTTGGGCCGAAGGGTCTTGCAGAGGTTGCTCGAACACTTGGAAAATCTCTCCGGGCATTTCAACCCACAATAAAAGAGCTTCAGGAAGTATCAAGGGAGTTCAAGAACACACTGGAGCAGGAAATTGGTCTAGATGAATTCCGTTCTACTACTATCAATCCCTCCAATTCTGCTCCTCCAACCTTGCAGCGTGAAACATTGGTGGAACAGAATGGGCCTCCTGGCAGAAAACCATACTCCGCTGAAGATTACTTGAGAGTTTCCCAAGAAGAAATAAAAGCATTAGTTCCAGAAGAGAAACCTAAGGGTTCAGAACCTGAACAGTTGGAGTAA___TAGCCCTCCCTCGGTTGCACAATCAGGATCTGAAGTGACTAAGCAATCGAGTTCTGAATTTCCTGAGCTTTCAACAAATTAGAACTGGCAGTGTGAATGGGAAGGGCGAGGATGGAAAGGATATGTAAATTGAAGGTCTGTTGGCTGCGGTGTATTGCTTTTGTTCTTGTAAAATATATTCTACAATGTATATCTGTAAAGGGCTTATGATTCTGCTAGATGCTTTCTATGGTTTGAGGTATCCGTGGCTTCCAATTTTGAAACTTCTGCTCCCATGTGGATCGCTGATCTTGTGAGATTCTTGTTATGCAACCTCCTTACAATTTTCTATCACTCTCTATTTGACAGTTCTTTTCCAGGGTCATTTTGCACGATCAAGGCTGTAGTCCAAGTCTAGAGAATATATCAAAGTTAAGGCCACTTGACACAAAGTGAGAAATTAAGATAAGCTCCTAATCAGGTCATATTCAGTCCGCCCGCTATTTCTGAATTGGGTTATATTCAGACTGACAACTAAGTTAAAGGAAGTCCACTCTTTGTGGTGCAGCCCTTGGTTCACTTGAAATGAAAGAAACCATTTTGAGTATTAATTTATTTTATATTTGCCATAAAGCATTAAATACTTAATGCTCTTTAGCTTTACATGCATAGTTCATTAAAAATCAATGGTAGCTTCAAAGTTCGCAATTTTTACAAAATTCAAAGATGAGAAGGTTAAGTTGCTCAAGGCTT

Fln Alignment:

Contig2921___MATAGPWALSSPCLC-----SSVFPTIGFRSKHKWVSSGLSSQHLCRATHTQKAHFEPLDRTRIAFFIGWNARTHLGQSFLFNAPNRGFHRQKKGSGVQASLFGVGAPEALVIGVVALLVFGPKGLAEVARTLGKSLRAFQPTIKELQEVSREFKNTLEQEIGLDEFRSTTINPSNSAPPTLQRETLVEQNGPPGRKPYSAEDYLRVSQEEIKALVPEEKPKGSEPEQLE
A9P230______________MTMAGPWALSSPCLCKPPHDSSVFPTISSHSNHKWVSSMLSSQHLCRATHAQKAHFETLYRTRLAFFIGWNARTHLGQSFLLNAPSRGFHRQKKGSAVQASLFGVGAPEALVIGVVALLVFGPKGLAEVARTLGRSLRAFQPTIKEIQEVSREFKNTLEQEIGLDEFRSTTINPSNSSPQTLQRETMVEQNGPPGRKAYSTEDYLRVSEDEIKTLVPEEKPKGSEPAQLD

SNPs (tot: 57)

Position	A %	C %	G %	T %	Probability
154	25	75			0.996473
155	25		75		0.996473
220			80	20	0.995493
517	18			81	0.999994
602		90		10	0.90298
765	12		87		0.992554
766	87		12		0.992554
898		11	88		0.923564
899	88		11		0.923564
905	88	11			0.923564
906			88	11	0.923564
909	87		12		0.992554
913	87		12		0.992554
914			87	12	0.992554
917	87	12			0.992554
944	12			87	0.992554
945	12	87			0.992554
970		20	80		0.999996
972	80			20	0.999996
973	80	20			0.999996
976	20		80		0.999996
978		20	80		0.999996
979	80			20	0.999996
980			80	20	0.999996
982			20	10	0.999998
983	80	20			0.999996
986			80	20	0.999996
987	80	20			0.999996
989	77	22			0.999999
990			77	22	0.999999
991		22	77		0.999999
1006		10	90		0.90298
1007			90	10	0.90298
1015	11		88		0.923564
1017		11		88	0.923564
1018			88	11	0.923564
1019		88		11	0.923564
1023		11	88		0.923564
1025	88		11		0.923564
1027		11		88	0.923564
1028	11		88		0.923564
1031		14		85	0.993533
1032	14			85	0.993533
1035		14		42	1.0
1091		14	85		0.993533
1291			87	12	0.992554
1315		11		88	0.923564
1316	88		11		0.923564
1317	88			11	0.923564
1318		11	88		0.923564
1319	88		11		0.923564
1321	88		11		0.923564
1323		11	88		0.923564
1326		88	11		0.923564
1330	88		11		0.923564
1335		11	88		0.923564
1462		20		80	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain