SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene2867

Length: 1348 nt

Origin of reads: Uagpf v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene2867

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Expansin-B16 n=2 Tax=Oryza sativa RepID=EXB16_ORYSJ	-	-	46%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	43%
Blast2go	expansin b1	-	-	64%

Source	GOs	Term	Type	e value	Identity
Blast2go	plant-type cell wall organization	GO:0009664	Biological Process	0.0	64%
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	0.0	64%

Source	InterPros	Term	Type	Identity
Sma3	Peptidase aspartic, active site	IPR001969	-	-
Sma3	Lipocalin	IPR002345	-	-
Sma3		IPR005132	-	-
Sma3	Major pollen allergen Lol pI	IPR005795	-	-
Sma3	Expansin/pollen allergen, DPBB domain	IPR007112	-	-
Sma3	Pollen allergen/expansin, C-terminal	IPR007117	-	-
Sma3	Expansin/Lol pI	IPR007118	-	-
Sma3		IPR014734	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A9L6

Fln msg:

Fln protein: MKWNLYVGYLVALGACLLITICSHTALGVRVGKRHPAAVEGEGAGWQPAAARWTGPADGAGSEGGACGYGSLVKQFPFDSKVASASPDLYKGGKGCGSCYQVKCTGNPLCSETGVTVVVADESYGQSQFVLSGTAFQDMASDPRRGTDLLNAGVVSALFRRVPCEYQRTNIAFHVNSGSSAYWLGLLVEYVNGDGDLGAVDLKQANSNTWQPMQQTSGANWALNSGPLSAPFSIRLTTLSTGKTLIIDNAIPENWQPNATYNTNANFH*

Protein Length: 270

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGCGGAGCAGAACCCCAGAGTTGCGCCGGTTGGGTTGGGATCAACAGTTTTGCACCTAATTCTCGCAATACTACTGTTACTATTGCCTCAGACAAA_-_ATGAAGTGGAACCTTTACGTGGGTTACTTGGTAGCGCTGGGAGCTTGCTTATTGATAACGATATGCTCTCACACCGCCTTGGGCGTGCGTGTGGGGAAACGCCATCCAGCGGCGGTTGAGGGGGAAGGCGCAGGTTGGCAGCCCGCCGCTGCAAGGTGGACTGGCCCTGCAGATGGCGCCGGAAGCGAAGGCGGTGCATGCGGATATGGTAGCCTTGTGAAACAGTTTCCTTTTGATTCAAAAGTGGCTTCAGCAAGTCCAGATCTGTACAAAGGAGGAAAAGGCTGTGGATCATGCTATCAGGTAAAATGCACCGGCAATCCTCTGTGTTCCGAAACGGGAGTGACCGTTGTGGTCGCTGACGAATCATATGGGCAATCACAGTTTGTTCTCAGCGGTACTGCCTTCCAAGATATGGCGTCTGATCCTCGTAGAGGTACAGATCTGCTCAATGCTGGAGTCGTGTCTGCTTTGTTTCGAAGGGTGCCTTGTGAATATCAGAGAACCAATATTGCCTTCCACGTCAATTCGGGCTCCAGTGCCTACTGGCTGGGGCTCCTTGTTGAATACGTAAATGGAGATGGCGACCTTGGAGCGGTCGATTTAAAACAGGCAAACTCCAATACATGGCAACCAATGCAACAAACATCGGGTGCAAATTGGGCTTTGAACAGTGGTCCTCTGAGTGCTCCCTTCTCCATTCGCCTTACAACCCTCTCCACAGGCAAAACTCTCATCATAGATAACGCTATTCCAGAGAATTGGCAGCCAAATGCTACGTACAATACCAATGCCAACTTTCATTGA___AAGCAAGCCATGAAATATATTTAGATGCAGAAATTCAAACGATTCAGAGGATATCTACAATAATAATGCGACCCTTTCCTGCTCTGAGCGCTGCTCACTCACTCATTCATTCATTCATGATGGGCGGATAAAAATCCAAAGCACGGCGCAGATAGTATCTAGTACTTGTCATGATTGCCGCACTGCAGTTATGTGATCTGAACTGAGCTGAGCTGAGCTGCCGATTAGCCCGGTGGCATGTGTATTTCTCCTTGGAATAAAGTTTAATCACTGAGCAGGAATAAAATGAAATGTTCATGATATGATATGTGATTCCGAATTATAATAAGTAATCTCGTTTTGCGGCAATTTCGAATTCATCCATTGCGAGTTTCTTAAACCATTGTGTGCATTTCATTTTATTGTGCATCTAACAATTGTGT

Fln Alignment:

Contig2867___TVTIASDKMKWNLYVGYLVALGACLLITICSHTALGVRVGKRHPAAVEGEGAGWQPAAARWTGPXXXXXXXXXXXXXXSLVKQFPFDSKVASASPDLYKGGKGCGSCYQVKCTGNPLCSETGVTVVVADESYGQ-------SQFVLSGTAFQDMASDPRRGTDLLNAGVVSALFRRVPCEYQRTNIAFHVNSGSSAYWLGLLVEYVNGDGDLGAVDLKQANSNTWQPMQQTSGANWALNSG-PLSAPFSIRLTTLSTGKTLIIDNAIPENWQPNATYNTNANF
D5A9L6______________TVSLQWPLLVSNVCVDVLATCFVLLLLFSCKEAAVAL-----DHTELRGNAMEWRSAIATWYGSPDGDGSDGGACGYGTLVDQKPLRATVGAVSPVLFHGGEGCGACYKVKCMEKGLCSQKPVTVVITDVCGGGYSAFGGGTHFDLSGAAFGRMATHGKTPA-LLNSGELPVLYRRTFCKYPGKNISFHVTEGSTVYWFSILIEYEDGDGDVGAVHLKQVDSNAWMKMRHIWGVNWFLPGGAPLKAPFSVRVTTLTSKRTLSAMNVIPEHWYPNATYSARVKF

SNPs (tot: 78)

Position	A %	C %	G %	T %	Probability
111		90	10		0.90298
116	90			10	0.90298
117		90	10		0.90298
119	90	10			0.90298
120	90	10			0.90298
121	90			10	0.90298
125	90		10		0.90298
126		10	90		0.90298
129			90	10	0.90298
130	90	10			0.90298
134	10			90	0.90298
137	90			10	0.90298
344	88			11	0.923564
382		11	88		0.923564
383	88		11		0.923564
387			88	11	0.923564
388		11		77	0.968132
389	88		11		0.923564
391	87	12			0.992554
392	75	12		12	0.975346
393	87			12	0.992554
396	87	12			0.992554
397			87	12	0.992554
400	87			12	0.992554
401	87	12			0.992554
403		12	87		0.992554
404			87	12	0.992554
407	12		87		0.992554
432		85		14	0.993533
448		30		70	1.0
451		30		70	1.0
452		70	30		1.0
453		70		30	1.0
457	70	30			1.0
458	70			30	1.0
459		70		30	1.0
462		30	70		1.0
463	62			37	1.0
466		30	70		1.0
471	20		70		0.999998
472	10			90	0.90298
553			86	13	0.999973
637		90	10		0.90298
657		85	14		0.999981
662	85	14			0.999981
667		78		21	1.0
782	90	10			0.90298
783	90		10		0.90298
785		10		90	0.90298
788	10		90		0.90298
790		10		90	0.90298
795	90		10		0.90298
797	64	28		7	1.0
802	28			71	1.0
806		71		7	1.0
809	71		21	7	1.0
810		21	78		1.0
812	7		78	14	0.999974
814	14			85	0.999981
817	14	85			0.999981
829		90	10		0.999898
832		5	10	85	0.999793
833	84		5	10	0.999846
946	95	4			0.996252
950	93	4	2		0.983471
952		4		95	0.99692
955		4	95		0.997218
968	67	32			1.0
1003		86		13	1.0
1065	63			36	1.0
1093	4		95		0.996599
1128	27			72	1.0
1133	94		5		0.997984
1142	94	5			0.998744
1144	5	94			0.998744
1146			94	5	0.998744
1150			6	87	0.993531
1242			85	14	0.993533

SSRs (tot: 2)

Start position	End position	Sequence	Length
1029	1044	TCAT TCAT TCAT TCAT	16
1129	1146	CTGAG CTGAG CTGAG CTG	18

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain