SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene2816

Length: 1960 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene2816
GTGTCATACTTGTAAGCCACGTCGTGACCTTGGCTGTCACTGGATTACAAATTAAGCCAACTCGGAGAAACGAGGGAGCCGATATGTGGTTGTGTTACTTGGACTGTGAAATCTAGTGAAACGCTGCAGTCAATTGGCAAGGAATCGACTTTGGCTTAGAATCCACGGATTCTATTTACTATATATATTGTTATCAATCTACAGCAAAGGAGAAACTTAAAATTGATCTCACAAAATATAATTTTGCTGAAACAAATCTGAGAAAAGAAAATTTTATAGGACACGATGGCGGAGCCTCCACCATTTCAAGAGGCTGCGCGCTGTGATGTATGCAAGTGTAGTTTCAACGCATTCAGGCGTCGGCACCATTGTCGTTGCTGTGGTAGGACTTTATGCTTTGAGCATTCATCAAAGCAAATTCCGTTGCCACAGTTTGGAATACATTCTGCTGTTAGAGTATGCCATGACTGCTTCAACAATTCTACACAGTCATCTTCAGCAAATACACAAACCAACTCCTATGGCATTGATAAAATGGCCTCAGATGACGTTGACGCAACAGTTTCTGATGGCATTGATGCATCCACTGATAAATTATCCAGAGTTGATTTGAATGAAGTCATTTCACTATCAAAATCAGAAAAAAGCTCTTATGTGGGAGGAAACTTGAGTATTTCTCAGACAGTTGAATGCAAATGTGGAATGCCATTATGCATATGTGAAGCTCCGGCTCCTCCTCAGGTCCAGCCTGAAGTGTCAACATCTCAGTATTATCAGCGATCAAAAAAGGTTGTTCCTGCCCAACATACTCCATCTGATACGATCTCGAAGTCAACTGCAGGCTCATCTGGAAGTATTAGGCAAAGTTTATTCTTTACAAGTGGGCAAACAAATAATGTATCCACGGACAAAAGTTCTAGTAGTTATGAGACTAGTGGAGAGGGGTTAAGGGAAGCCATCAAGAACAATGATATATCTGCCGTCAAAGATCTTCTAATGCAGGGAGTAGATGCCAACTATTGTGACAAACAAGGAATGTCTTTATTGCATTTGGCTACAGTTTTTAATCACACAGAAATTGCATTTATTCTGATGGATGCTGGAGCTAGTGTGGATGCAAAAAATGCACAAGGGGAAACGCCACTGGATTGCGCACCAACTATGTTACAGTACAAAATGCATCAGAAAATACAGGCTTCTAGCATGTAGCAGTTAATTGACGGTTTTATTACTTAGCAATTTTACAGTTTTCATAACATTTTTATGGAACAGCAGTTATGCTGTTCAGTAGTTGTGTACTCTAGGATCCGTATTCATTATTAATGCGCCTTGTGATTGCGTGGTTCTGACAAAATCAAGAAAATTTGTCACCTATTATATTCCCTTAGTAATACTGGTCTTTTTCTTTCATGGCCTTTTCTATTGTGAAAGGTATGAAAGTCTTGGGATATTTTACGCATAAATGTAGCAGAAAGAGAATTATTTTTCTACGAGGAGACTGATGACTGCTCCACTGTTGAAGCTGTATTGCTTGAACTTTGAGTGGTGATGATATAGTCGGGTTCTCAACCAGAATTTGTGATGGGGTGTGCATATAACTGGGGACAAGCTGCAACATTTGGAATTGAAGTTTGGGGACTGAAAAGTTTAGCCCACTCTGAATTCAGTTTGTCATTAGCATGCAAATATAGTTAGAGTGAATGATAAACACTTTAGAGTGTGGTTGCCCGGTTACGTGGCTATGGGGATTCTGGATACGCCAGATACAGGCATGGAGTACATGGATATAAATCCCCAAATATTGGATATATTGATACAAAGATACTACTAATATGATGCCCACTGGCACATATATCCATGTACTGCAGAAAATTATTCTAATTTCAGATTCAACTTTCAAGCTTAAACCTTAAAATTAGAAATTCAATAATCAAAAGAGTTCCCTCAGTGTTCATTTCAC

Ace file of the UniGene sp_v2.0_unigene2816 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Ankyrin repeat family protein [Arabidopsis thaliana] gb\|AAL38772.1\| unknown protein [Arabidopsis thaliana] gb\|AAM20189.1\| unknown protein [Arabidopsis thaliana] gb\|AEC07808.1\| Ankyrin repeat family protein [Arabidopsis thaliana]	-	-	1.0e-33	41%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	92%
Blast2go	protein	-	-	0.0	63%

Source	GOs	Term	Type	e value	Identity
Blast2go	zinc ion binding	GO:0008270	Molecular Function	0.0	63%

Source	InterPros	Term	Type	Identity
Sma3	Zinc finger, FYVE-type	IPR000306	-	-
Sma3	Ankyrin repeat	IPR002110	-	-
Sma3	Zinc finger, FYVE-related	IPR017455	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ACK5

Fln msg:

Fln protein: MAEPPPFQEAARCDVCKCSFNAFRRRHHCRCCGRTLCFEHSSKQIPLPQFGIHSAVRVCHDCFNNSTQSSSANTQTNSYGIDKMASDDVDATVSDGIDASTDKLSRVDLNEVISLSKSEKSSYVGGNLSISQTVECKCGMPLCICEAPAPPQVQPEVSTSQYYQRSKKVVPAQHTPSDTISKSTAGSSGSIRQSLFFTSGQTNNVSTDKSSSSYETSGEGLREAIKNNDISAVKDLLMQGVDANYCDKQGMSLLHLATVFNHTEIAFILMDAGASVDAKNAQGETPLDCAPTMLQYKMHQKIQASSM*

Protein Length: 309

Fln nts: GTGTCATACTTGTAAGCCACGTCGTGACCTTGGCTGTCACTGGATTACAAATTAAGCCAACTCGGAGAAACGAGGGAGCCGATATGTGGTTGTGTTACTTGGACTGTGAAATCTAGTGAAACGCTGCAGTCAATTGGCAAGGAATCGACTTTGGCTTAGAATCCACGGATTCTATTTACTATATATATTGTTATCAATCTACAGCAAAGGAGAAACTTAAAATTGATCTCACAAAATATAATTTTGCTGAAACAAATCTGAGAAAAGAAAATTTTATAGGACACG_-_ATGGCGGAGCCTCCACCATTTCAAGAGGCTGCGCGCTGTGATGTATGCAAGTGTAGTTTCAACGCATTCAGGCGTCGGCACCATTGTCGTTGCTGTGGTAGGACTTTATGCTTTGAGCATTCATCAAAGCAAATTCCGTTGCCACAGTTTGGAATACATTCTGCTGTTAGAGTATGCCATGACTGCTTCAACAATTCTACACAGTCATCTTCAGCAAATACACAAACCAACTCCTATGGCATTGATAAAATGGCCTCAGATGACGTTGACGCAACAGTTTCTGATGGCATTGATGCATCCACTGATAAATTATCCAGAGTTGATTTGAATGAAGTCATTTCACTATCAAAATCAGAAAAAAGCTCTTATGTGGGAGGAAACTTGAGTATTTCTCAGACAGTTGAATGCAAATGTGGAATGCCATTATGCATATGTGAAGCTCCGGCTCCTCCTCAGGTCCAGCCTGAAGTGTCAACATCTCAGTATTATCAGCGATCAAAAAAGGTTGTTCCTGCCCAACATACTCCATCTGATACGATCTCGAAGTCAACTGCAGGCTCATCTGGAAGTATTAGGCAAAGTTTATTCTTTACAAGTGGGCAAACAAATAATGTATCCACGGACAAAAGTTCTAGTAGTTATGAGACTAGTGGAGAGGGGTTAAGGGAAGCCATCAAGAACAATGATATATCTGCCGTCAAAGATCTTCTAATGCAGGGAGTAGATGCCAACTATTGTGACAAACAAGGAATGTCTTTATTGCATTTGGCTACAGTTTTTAATCACACAGAAATTGCATTTATTCTGATGGATGCTGGAGCTAGTGTGGATGCAAAAAATGCACAAGGGGAAACGCCACTGGATTGCGCACCAACTATGTTACAGTACAAAATGCATCAGAAAATACAGGCTTCTAGCATGTAG___CAGTTAATTGACGGTTTTATTACTTAGCAATTTTACAGTTTTCATAACATTTTTATGGAACAGCAGTTATGCTGTTCAGTAGTTGTGTACTCTAGGATCCGTATTCATTATTAATGCGCCTTGTGATTGCGTGGTTCTGACAAAATCAAGAAAATTTGTCACCTATTATATTCCCTTAGTAATACTGGTCTTTTTCTTTCATGGCCTTTTCTATTGTGAAAGGTATGAAAGTCTTGGGATATTTTACGCATAAATGTAGCAGAAAGAGAATTATTTTTCTACGAGGAGACTGATGACTGCTCCACTGTTGAAGCTGTATTGCTTGAACTTTGAGTGGTGATGATATAGTCGGGTTCTCAACCAGAATTTGTGATGGGGTGTGCATATAACTGGGGACAAGCTGCAACATTTGGAATTGAAGTTTGGGGACTGAAAAGTTTAGCCCACTCTGAATTCAGTTTGTCATTAGCATGCAAATATAGTTAGAGTGAATGATAAACACTTTAGAGTGTGGTTGCCCGGTTACGTGGCTATGGGGATTCTGGATACGCCAGATACAGGCATGGAGTACATGGATATAAATCCCCAAATATTGGATATATTGATACAAAGATACTACTAATATGATGCCCACTGGCACATATATCCATGTACTGCAGAAAATTATTCTAATTTCAGATTCAACTTTCAAGCTTAAACCTTAAAATTAGAAATTCAATAATCAAAAGAGTTCCCTCAGTGTTCATTTCAC

Fln Alignment:

Contig2816___MAEPPPFQEAARCDVCKCSFNAFXXXXXXXXXXRTLCFEHSSKQIPLPQFGIHSAVRVCHDCFXXXXXXXXXXXXXXSYGIDKMASDDVDATVSDGIDASTDKLSRVDLNEVISLSKSEKSSYVGGNLSISQTVECKCGMPLCICEAPAPPQVQPEVSTSQYYQRSKKVVPAQHTPSDTISKSTAGSSGSIRQSLFFTSGQTNNVXXXXXXXXXXXXXXGLREAIKNNDISAVKDLLMQGVDANYCDKQGMSLLHLATVFNHTEIAFILMDAGASVDAKNAQGETPLDCAPTMLQYKMHQKIQASS
D5ACK5______________MAEPPPFQEAARCDVCKCSFNAFRRRHHCRCCGRTLCFEHSSKQIPLPQYGIHSAVRVCHDCFNNATQSSSANTQTNSNDIDKMASDNIDARASDGIDASTDKLSR------ISLSKSENSSYVGGNLSVSQTVECKCGMPLCICEAPAPPQVQPEVSTSQYYQRPKKVVTAQHTPSDTISKSTAGSSGSTRQSLFFTSGQTSNVSTDKSSRGYETSGEGLREAIKNNDISAVKDLLMQGVDANYCDKQGMSLLHLATVFNHTEIAFILMDAGASVDAKNAQGETPLDCAPTMLQYKMHQKIQEAS

SNPs (tot: 76)

Position	A %	C %	G %	T %	Probability
34	25			75	0.996473
37	75			25	0.996473
52	20		80		0.995493
88	83		16		0.994512
89	16			83	0.994512
111		12		87	0.992554
628		83	16		0.999991
636	10		90		0.90298
637	10	90			0.90298
638	77		11		0.968132
639			11	77	0.968132
640		11		88	0.923564
641	88	11			0.923564
643		11		77	0.968132
644	77		22		0.999999
645	88		11		0.923564
646		11		88	0.923564
647		11	88		0.923564
649	90	10			0.90298
650			10	90	0.90298
652		70		30	1.0
681			88	11	0.923564
682		88	11		0.923564
685	88		11		0.923564
688	11			88	0.923564
690	12	75			0.975346
691	12	12		75	0.975346
694	14		14	71	0.984337
695	28	71			1.0
696		85	14		0.993533
697	83		16		0.994512
699	66	16	16		0.988813
700	83	16			0.994512
701	66	33			1.0
702	83		16		0.994512
704		16	16	66	0.988813
707		20		80	0.995493
709		20		80	0.995493
712	80	20			0.995493
713	80		20		0.995493
732	75		25		0.996473
734		25		75	0.996473
736	50		25		0.995533
737	75	25			0.996473
738	75	25			0.996473
739	50	50			1.0
1751		90		10	0.90298
1753		90		10	0.90298
1754		90	10		0.90298
1777	84		15		0.999986
1888	11	88			0.923564
1893			12	87	0.992554
1894	12			87	0.992554
1895			12	87	0.992554
1896	12	87			0.992554
1897	12			87	0.992554
1898	12	87			0.992554
1903			12	87	0.992554
1904			12	87	0.992554
1910	85	14			0.993533
1911	85	14			0.993533
1912		14		85	0.993533
1913		14		85	0.993533
1920	20	40		40	1.0
1921		40	40		1.0
1922	40	40	20		1.0
1923	40		60		1.0
1928	66		33		0.997452
1930		66		33	0.997452
1932	66		33		0.997452
1933	66			33	0.997452
1934		33	66		0.997452
1935			66	33	0.997452
1937		66		33	0.997452
1938	66			33	0.997452
1939		66	33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain