SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene2417

Length: 2051 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene2417
ATGGGAAGCAACCAACCATTACAGGCCTTCAGGTTTATACACTTCAGTTATTGGTTCTTCATGGTAAACTAATGTTTTCTCCATTCTCATTCCCATTGCATTTGTGGAGCTGCAGGTTCTCAGAAGGGTTGTAGAACTCTTCCTCTGGGGTTTCTGCCTGATTCATTAATTGATAATATCAAAGGCATCATGGAGAGAAGAGAGTTAAAGTCTTCAGAGGGACGAAGTTGTATGCAGAATTGCCATATGTTGATCAGGAATGCAGCTGGGTTTTTCTTGGCATTAACTATCTTGGCTATTGCATTTTCACCTGCAGGTGCTTATGATCCACTGGATCCAAATGGTAACATAACGATCAAATGGGATATTGTGTCTTGGACTGCAGATGGATATGTTGCGGCCGTGACCATGCACAATTTTCAGCAGTACCGCCATATACCAAGCCCCCAGGATGGACTTTGGGTTGGCACTGGGCAAAAAAGGAGGTCATCTGGGCTGTGGTTGGTGGCCAGACAACAGAGCAAGGAGATTGCTCCAAATGGAAAGCAGGTGTTCCACACTGTTGCAAGAAGGACCCCACCGTTGTTGATCTCTTACCAGGTGTGCCTTACAATCAGCAATTTACTAATTGCTGCAAAGGTGGAGCGTTGGCATCTTGGGCTCAGGATCCTCCAAATGCTATTGCATCCTTCCAAGTTAGTGTGGGTAATTCAGGGACCACTAACAAAACTGTCAAATTGCCCAAGAATTTCACTTTGAAGGCTCCAGGACCGGGTTATACTTGTGGCCCTGCTAAGATTGTTAAATCCAGCCTGTTCTTTAGTCCAGACCGCCGCAGAACCACTCAAGCTCTGATGACATGGAATGTAACATGCACATATTCTCAGTTCCTGGCTCAGAAAAGTTCAACATGCTGCGTCTCCTTATCATCGTTCTATAACGATACTATAACACCATGTCCTACTTGCGCCTGCGCTTGCCGGAACAACATAACTCAACCTGCTTGTATTGATACCGATTCACCAGTTCTTAAGTTGCCCGGGACAAATTCTGCGACAAACACCTTGCAGCCACCACTTCTACGTTGCACCCGTCATATGTGCCCTATCAGAGTACACTGGCATGTGAAGTTGAATTACAAAGAGTACTGGAGAGTGAAGATCACAGTGACAAATTTCAATTACAGAATGAACTACTCCGACTGGACATTAGTTGCACAGCATCCAAACTTTGATAATGTAACCCAAGTTTTCAGCTTCAACTATAAATCTTTAACTCCATATGGATCGATCAACGATACTGCCATGTTCTGGGGACAGAAATATTATAATGATCTGCTTATGCAGGCAGGAGCGATGGGAAGTGTGCAATCTGAGATATTACTCCGGAAGGACAAGAAAACGTTCACCTTCAAACAAGGGTGGGCATTCCCTCGGAGATTGTACTTCAATGGAGACCAGTGCGTGATGCCATCACCAGATGCATATCCCTGGCTGCCCAGCACCGCTCACCCTGCTCCTGCCCCTCCATCATTGTTTGCTTTCATTCTTCCACTTGCTCTTGCCATTGCAATTGCAATTGCACCTTCTTTATTCCTGCCATAGCCATTTACAGACTCTCATCTGCTAAGGCACCTGTTTAGGTAGCAATTTACATATTCTGCTATGCTATACTATACTCTGTAAATGCTCCTGTACTCTCAACAATTGCAAATTTACATATTCTGCCATTCTTTTGAGGAATTACACATAATATTTTTAGTGGAATGTAGTGTCGTTGGTAGATTTTGATCATGCTCAAAGTTGAACACTGTCTTCTGTACTGTATCTGGGCAGTCAGAGATATGAGGCATGACATTTTGGTCACTGGTAAAGGGCATTTGTAAAAACCTGATTCCAACATTTCTCTGAATCACCCACCCTATGTGAAGGAGAAGGTTCTTGGTTTTATATTTTTCATGTCCATCACAGAGGGTAGTTATTAAAGTTTTCTGTCTGGGGACTTTTGATATATTTATAAATAAAAACCCAGTTGGCAAACTGTATGTTGGC

Ace file of the UniGene sp_v2.0_unigene2417 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Ch-cobra n=1 Tax=Cunninghamia lanceolata RepID=B7S746_CUNLA	-	-	73%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	72%
Blast2go	protein cobra-like	-	-	85%

Source	ECs	Term	Type	e value	Identity
Blast2go	Ubiquinol--cytochrome-c reductase.	EC:1.10.2.2	-	0.0	85%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Oxidative phosphorylation	00190		0.0	85%
Blast2go	Metabolic pathways	01100		0.0	85%

Source	GOs	Term	Type	Identity
Blast2go	multidimensional cell growth	GO:0009825	Biological Process	85%
Blast2go	response to salt stress	GO:0009651	Biological Process	85%
Blast2go	cellulose microfibril organization	GO:0010215	Biological Process	85%
Blast2go	aerobic respiration	GO:0009060	Biological Process	85%
Blast2go	ubiquinol-cytochrome-c reductase activity	GO:0008121	Molecular Function	85%
Blast2go	external side of plasma membrane	GO:0009897	Cellular Component	85%
Blast2go	mitochondrial respiratory chain complex III	GO:0005750	Cellular Component	85%
Blast2go	plant-type cell wall	GO:0009505	Cellular Component	85%
Blast2go	longitudinal side of cell surface	GO:0009930	Cellular Component	85%
Blast2go	anchored to plasma membrane	GO:0046658	Cellular Component	85%

Source	InterPros	Term	Type	e value	Identity
Sma3	COBRA-like	IPR017391	-	0.0	-
Sma3	Glycosyl-phosphatidyl inositol-anchored, plant	IPR006918	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NXV9

Fln msg: Overlapping hits, possible frame ERROR between 528 and 414,

Fln protein: MERRELKSSEGRSCMQNCHMLIRNAAGFFLALTILAIAFSPAGAYDPLDPNGNITIKWDIVSWTADGYVAAVTMxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCSKWKAGVPHCCKKDPTVVDLLPGVPYNQQFTNCCKGGALASWAQDPPNAIASFQVSVGNSGTTNKTVKLPKNFTLKAPGPGYTCGPAKIVKSSLFFSPDRRRTTQALMTWNVTCTYSQFLAQKSSTCCVSLSSFYNDTITPCPTCACACRNNITQPACIDTDSPVLKLPGTNSATNTLQPPLLRCTRHMCPIRVHWHVKLNYKEYWRVKITVTNFNYRMNYSDWTLVAQHPNFDNVTQVFSFNYKSLTPYGSINDTAMFWGQKYYNDLLMQAGAMGSVQSEILLRKDKKTFTFKQGWAFPRRLYFNGDQCVMPSPDAYPWLPSTAHPAPAPPSLFAFILPLALAIAIAIAPSLFLP*

Protein Length: 473

Fln nts: ATGGGAAGCAACCAACCATTACAGGCCTTCAGGTTTATACACTTCAGTTATTGGTTCTTCATGGTAAACTAATGTTTTCTCCATTCTCATTCCCATTGCATTTGTGGAGCTGCAGGTTCTCAGAAGGGTTGTAGAACTCTTCCTCTGGGGTTTCTGCCTGATTCATTAATTGATAATATCAAAGGCATC_-_ATGGAGAGAAGAGAGTTAAAGTCTTCAGAGGGACGAAGTTGTATGCAGAATTGCCATATGTTGATCAGGAATGCAGCTGGGTTTTTCTTGGCATTAACTATCTTGGCTATTGCATTTTCACCTGCAGGTGCTTATGATCCACTGGATCCAAATGGTAACATAACGATCAAATGGGATATTGTGTCTTGGACTGCAGATGGATATGTTGCGGCCGTGACCATGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTGCTCCAAATGGAAAGCAGGTGTTCCACACTGTTGCAAGAAGGACCCCACCGTTGTTGATCTCTTACCAGGTGTGCCTTACAATCAGCAATTTACTAATTGCTGCAAAGGTGGAGCGTTGGCATCTTGGGCTCAGGATCCTCCAAATGCTATTGCATCCTTCCAAGTTAGTGTGGGTAATTCAGGGACCACTAACAAAACTGTCAAATTGCCCAAGAATTTCACTTTGAAGGCTCCAGGACCGGGTTATACTTGTGGCCCTGCTAAGATTGTTAAATCCAGCCTGTTCTTTAGTCCAGACCGCCGCAGAACCACTCAAGCTCTGATGACATGGAATGTAACATGCACATATTCTCAGTTCCTGGCTCAGAAAAGTTCAACATGCTGCGTCTCCTTATCATCGTTCTATAACGATACTATAACACCATGTCCTACTTGCGCCTGCGCTTGCCGGAACAACATAACTCAACCTGCTTGTATTGATACCGATTCACCAGTTCTTAAGTTGCCCGGGACAAATTCTGCGACAAACACCTTGCAGCCACCACTTCTACGTTGCACCCGTCATATGTGCCCTATCAGAGTACACTGGCATGTGAAGTTGAATTACAAAGAGTACTGGAGAGTGAAGATCACAGTGACAAATTTCAATTACAGAATGAACTACTCCGACTGGACATTAGTTGCACAGCATCCAAACTTTGATAATGTAACCCAAGTTTTCAGCTTCAACTATAAATCTTTAACTCCATATGGATCGATCAACGATACTGCCATGTTCTGGGGACAGAAATATTATAATGATCTGCTTATGCAGGCAGGAGCGATGGGAAGTGTGCAATCTGAGATATTACTCCGGAAGGACAAGAAAACGTTCACCTTCAAACAAGGGTGGGCATTCCCTCGGAGATTGTACTTCAATGGAGACCAGTGCGTGATGCCATCACCAGATGCATATCCCTGGCTGCCCAGCACCGCTCACCCTGCTCCTGCCCCTCCATCATTGTTTGCTTTCATTCTTCCACTTGCTCTTGCCATTGCAATTGCAATTGCACCTTCTTTATTCCTGCCATAG___CCATTTACAGACTCTCATCTGCTAAGGCACCTGTTTAGGTAGCAATTTACATATTCTGCTATGCTATACTATACTCTGTAAATGCTCCTGTACTCTCAACAATTGCAAATTTACATATTCTGCCATTCTTTTGAGGAATTACACATAATATTTTTAGTGGAATGTAGTGTCGTTGGTAGATTTTGATCATGCTCAAAGTTGAACACTGTCTTCTGTACTGTATCTGGGCAGTCAGAGATATGAGGCATGACATTTTGGTCACTGGTAAAGGGCATTTGTAAAAACCTGATTCCAACATTTCTCTGAATCACCCACCCTATGTGAAGGAGAAGGTTCTTGGTTTTATATTTTTCATGTCCATCACAGAGGGTAGTTATTAAAGTTTTCTGTCTGGGGACTTTTGATATATTTATAAATAAAAACCCAGTTGGCAAACTGTATGTTGGC

Fln Alignment:

Contig2417___IMERRELKSSEGRSCMQNCHMLIRNAAGFFLALTILAIAFSPAGAYDPLDPNGNITIKWDIVSWTADGYVAAVTMHNFQQYxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTTEQGDCSKWKAGVPHCCKKDPTVVDLLPGVPYNQQFTNCCKGGALASWAQDPPNAIASFQVSVGNSGTTNKTVKLPKNFTLKAPGPGYTCGPAKIVKSSLFFSPDRRRTTQALMTWNVTCTYSQFLAQKSSTCCVSLSSFYNDTIXXXXXXXXXCRNNITQPACIDTDSPVLKLPG-TNSATNTLQPPLLRCTRHMCPIRVHWHVKLNYKEYWRVKITVTNFNYRMNYSDWTLVAQHPNFDNVTQVFSFNYKSLTPYGSINDTAMFWGQKYYNDLLMQAGAMGSVQSEILLRKDKKTFTFKQGWAFPRRLYFNGDQCVMPSPDAYPWLPST
A9NXV9______________MMEKRELNSSRGQRCMQNCHILNKNAAGFFLALTILAIAFSPAGAYDPLDPNGNITIKWDIVSWTADGYVAAVTMHNFQQYxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTTEQGDCSKWKAGSPHCCKKDPTVVDLLPGVPYNQQFTNCCKGGALASWAQDPPNSVASFQVSVGNSGTTNKTVKLPKNFTLKAPGPGYTCGPAKIVKSSLFFSTDRRRTTQALMTWNVTCTYSQFLAQKSSTCCVSLSSFYNDTITPCPTCACACRNNVTQPSCVHSDSPVLKLPGPTNPITNSLQPPLLQCTRHMCPVRVHWHVKLNYKDYWRVKITVTNFNYRMNYTDWTLVAQHPNFDNVTQVFSFNYKPLTPYGSINDTAMFWGQKYYNDLLMQAGPMGSVQSELLLRKDKQTFTFKQGWAFPRRLYFNGDQCVMPSPDAYPWLPST

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
1365			91	8	0.999766
1428	86	4			0.999642
1487		11		88	0.923564
1530			12	87	0.992554
1534	12			87	0.992554
1536	14			85	0.993533
1537			85	14	0.993533
1579			33	66	0.997452
1633		33		66	0.997452
1634		33	66		0.997452
1635	66	33			0.997452
1637	66			33	0.997452
1813		62	37		1.0
1824	25		75		1.0
1827	75			25	1.0
1828		75		25	1.0
1867	40		60		1.0
1908		40	60		1.0
1937	40	60			1.0
1939		60	40		1.0

SSRs (tot: 1)

Start position	End position	Sequence	Length
469	486	TGCAAT TGCAAT TGCAAT	18

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain