SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene2320

Length: 1080 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene2320 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	[L] COG4581 Superfamily II RNA helicase	-	-	1.96182e-44	31%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	36%
Blast2go	superkiller viralicidic activity 2-like 2-like	-	-	0.0	79%

Source	GOs	Term	Type	Identity
Blast2go	RNA metabolic process	GO:0016070	Biological Process	79%
Blast2go	specification of floral organ identity	GO:0010093	Biological Process	79%
Blast2go	nucleic acid binding	GO:0003676	Molecular Function	79%
Blast2go	ATP-dependent helicase activity	GO:0008026	Molecular Function	79%
Blast2go	ATP binding	GO:0005524	Molecular Function	79%
Blast2go	RNA helicase activity	GO:0003724	Molecular Function	79%
Blast2go	nucleus	GO:0005634	Cellular Component	79%

Source	InterPros	Term	Type	Identity
Sma3	Helicase, C-terminal	IPR001650	-	-
Sma3	DNA/RNA helicase, DEAD/DEAH box type, N-terminal	IPR011545	-	-
Sma3		IPR012287	-	-
Sma3	DSH, C-terminal	IPR012961	-	-
Sma3	Helicase, superfamily 1/2, ATP-binding domain	IPR014001	-	-
Sma3		IPR014021	-	-
Sma3	RNA helicase, ATP-dependent, SK12/DOB1	IPR016438	-	-
Sma3	Myb-like domain	IPR017877	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: B8LMT3

Fln msg: Distance to subject end: 192 aas, your sequence is shorter than subject: 360 - 884

Fln protein: TALRKFDGDSHRWMTSGEYIQMSGRAGRRGKDGRGICIIMIDEQMDMKTCKEMILGKPAPLVSTFRLSYYTLLNLMSRAEGQFDSEYCIKNSFHQFQHDKALPEVEEKIAKLEQEAAMLGASEETSLADYHKLRLSIAELEKQMMTEITRPERVLLFLVPGRLIKVRDGTTEWGWGVVVNVVKKPSMPPSTLPSNILGSRTSSYIVDTLLHCASGLTSDGPRPKPCPPPPRQKGEMHVVPVQLPLICSLSNLRVAIPQDLRPAEARQSVLMAVQELEKRFPKGLPKLDPIEDMGIDDPEFVQLVKEVEALEEKLLAHPFHKSEQDEHQLRSFQRKAELNSAAQQLRMKMRDSQLHKFREE

Protein Length: 361

Fln nts: ACTGCACTTAGAAAATTTGATGGAGACAGCCATCGTTGGATGACTTCTGGTGAATATATTCAGATGAGTGGCAGAGCTGGACGGCGGGGAAAAGATGGACGTGGCATTTGTATTATTATGATTGATGAACAGATGGACATGAAGACTTGCAAGGAAATGATCTTGGGAAAACCTGCACCATTAGTTAGTACCTTCAGGTTGAGCTATTACACACTACTAAATTTAATGAGTCGAGCTGAGGGACAATTTGATTCTGAATATTGCATCAAGAATTCTTTCCATCAGTTTCAACATGATAAGGCACTACCAGAAGTTGAAGAGAAGATTGCAAAATTGGAGCAAGAAGCAGCCATGCTTGGTGCATCTGAGGAGACTTCTCTTGCAGATTATCATAAACTGAGACTAAGCATAGCAGAGTTAGAGAAGCAAATGATGACCGAAATTACTAGGCCTGAGCGCGTTCTTCTATTTCTGGTTCCTGGTAGGCTGATTAAAGTGAGAGATGGTACAACTGAGTGGGGATGGGGAGTGGTAGTTAATGTGGTGAAAAAGCCATCAATGCCACCCAGCACATTACCTTCTAATATATTGGGTTCTCGTACTAGTAGTTATATAGTGGATACTCTTTTACATTGTGCTTCTGGCTTGACAAGTGATGGTCCCCGCCCAAAGCCATGTCCTCCTCCTCCTAGACAAAAAGGTGAAATGCATGTGGTACCTGTTCAACTGCCCCTTATTTGTTCTCTTAGTAATCTTAGAGTTGCCATTCCACAAGATCTACGACCGGCAGAGGCAAGACAGAGTGTACTTATGGCAGTGCAAGAGCTGGAAAAACGATTTCCGAAAGGTCTTCCAAAGCTTGACCCTATTGAGGACATGGGCATTGATGATCCGGAATTTGTCCAACTTGTAAAGGAAGTTGAAGCATTGGAGGAAAAGTTGCTTGCCCATCCCTTTCATAAGTCTGAACAGGATGAACACCAGTTGAGGTCTTTTCAAAGAAAAGCAGAACTAAATAGCGCAGCTCAGCAACTTCGAATGAAAATGCGTGACTCTCAGCTTCACAAATTTAGGGAAGAG

Fln Alignment:

Contig2320___TALRKFDGDSHRWMTSGEYIQMSXXXXXXXXXXXGICIIMIDEQMDMKTCKEMILGKPAPLVSTFRLSYYTLLNLMSRAEGQFDSEYCIKNSFHQFQHDKALPEVEEKIAKLEQEAAMLGASEETSLADYHKLRLSIAELEKQMMTEITRPERVLLFLVPGRLIKV-------------RDGTTEWGWGXXXXXVKKPSMPPSTLPSNILGSRTSSYIVDTLLHCASGLTSDGXXXXXXXXXXRQKGEMHVVPVQLPLICSLSNLRVAIPQDLRPAEARQSVLMAVQELEKRFPKGLPKLDPIEDMGIDDPEFVQXXXXXXXXXXXXXAHPFHKSEQDEHQLRSFQRKAELNSAAQQLRMKMRDSQLHKFREE
B8LMT3______________TNVRKFDGDKFRWISSGEYIQMSGRAGRRGLDARGICILMVDEKMEPSTAKLMVKGSADPLNSAFHLSYNMLLNQMRCEDG--DPETLLRQSFYQFQSDRALPNLQKRVKDLEEQRDSIMIEEEDKLKDYYSLLEQFRSFKGDIRSIVFSPRHCLPFLQPGRLIRILSTSGEKDKLSFSQDEQLTWGVIVNFERVKSTSKDDTD-----VRPEDAQYTIDILTKCRAN-KDNSRGKIVQTVPLTEPGEPLVVAVPLSQIDSLSAIRIYIPKDLLSVEARENTLKKVQEVLNRFPDGVQLLDPEDDMQVQSSSYKKAVRRIETLEGLLAKHVVSKSPILQKRLQVLRKKEELTAMVRAARRQVRASTSLAFKDE

SNPs (tot: 43)

Position	A %	C %	G %	T %	Probability
350		11	88		0.923564
359	88		11		0.923564
451		16	83		0.994512
478			16	83	0.994512
487	80	20			0.995493
489		80	20		0.995493
494			20	80	0.995493
495	80		20		0.995493
498		20		80	0.995493
499	20			80	0.995493
500	80	20			0.995493
503	80	20			0.995493
515	25		75		0.996473
560	20			80	0.995493
561		80		20	0.995493
562		80	20		0.995493
563		80		20	0.995493
897	83		16		0.999991
899		83	16		0.999991
901	83		16		0.999991
903		16		83	0.999991
904	16		83		0.999991
907		16	83		0.999991
910	16		83		0.999991
913			16	83	0.999991
915		18		81	0.999994
916	18	81			0.999994
918		81	18		0.999994
920	81	18			0.999994
939		88	11		0.923564
969		22		77	0.999999
970	66	22	11		0.999999
972			11	88	0.923564
973	85			14	0.993533
974		85	14		0.993533
976	85	14			0.993533
978			14	85	0.993533
979		16	66	16	0.988813
980		83	16		0.994512
981	33	66			1.0
982	80		20		0.995493
983		60	20	20	0.99255
985	20			80	0.995493

SSRs (tot: 1)

Start position	End position	Sequence	Length
391	403	AGG AGG AGG AGG A	13

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain