SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene2146

Length: 2062 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene2146
CGTCGTGACCTTGGCTGTCACTCATCTCGATAGAAAGGCACTCAGAATAGTACAGTTGTGCCTTGCAGCGTCAGTCGCCTTCAACATTTCGAAGGAAACCACGACAGAGGGTTTGATGAAGGCGTTGTCGAAACTATATGAAAAACCCTCGGCCTCAAACAAGGTATTTCTTATGAAACGGTTATTTAATATGAAGATGTCGGAAGGTGGATCTGTTGCAGACCATTTAAATGATTTTAATACAGTTACAAGTCAGTTAAGTTCTGTAGGGGTAAACTTTGATGATGAAATTAGAGCTCTGTTATTCTTGTGCTCATTACCAGAAAGTTGGAATGGTTTGGTAATGGCCATTAGTAACTCCGTTTCTGGATCTAGTACTTTAAAGTTTGATGATGTTGTCGGTGCTCTCCTTAGCGAGGAAATGCGATGGAAAAGCTCTGGTGAGACATCGGGAAATGCTTTGAGTGCAGAGTCTAGAGGAAGAAAAATAGGAAAGAGGAAAGAGCTCAGGATATCGCAGTAAATCAAGGAAAGGCAGATCCAAGTCCAGATCGGGGATAGTGTGCTGGAAATGTGGAAAGAAAGGACACCTAAAGAAAGACTGTAGATCTCGGAAGGGAAAAGAAGGAGATGCACAACAAGAAAACAATCATGAAGCAAATGTGACAGGTGAGGTGTTACAAGATGCTTTGATTCTTTCTTTCGAAAATATTACTGATTCCTGGGTGGTAGATTCAGGGGCCTCATTCCATGCCACACCTGATAAGAAATACTTTCATGACTATGTTCAAGGAGATTTTGGACAAGTTCGTCTGGGTGATGATAAACCCTGTAAAATTGTTGGAATGGGTAAGGTTCTTGTAAAACAGCAGAATTGAAACCCATGGGTACTAAAGGAGGTAAGGCATGTTCCGGATCTAAAGAAAAATCTGATTTCAACAGGGCAGTTAGGCAGTGAAGGTTGTGTGACAACTTTCAGAGATAAAGCTTGGAAGGTCACCAAAGGAGCCCTGGTAATAGCAAAAGGTGAAAAGGTTGGTACATTGTATTTATGTAATGGTATTTCTAACTCTGTTAATGCTTTGACTTCCAAAGGAGCAGATGTAGCATTATGGCATCATAGGCTCGGACACATGATTGAAAAAGGGATGAAGATTCTTCACTCAAGGAATTTGTTGCCAGGTTTGAAGAATGTTGATTTGGATTTCTGTGAGAACTGCATTTATGGAAAACAGAAGAGAGTCAGATTTCTTAGAGTTGGGAAAGAAAAGAAGAGCGAAAAATTAGAGCTTGTGCATACAGATGTATGGGGACCTGCTCAGGTATCATCTCTCGGTGGCTCTCGTTATTATGTTACTTTTATTGATGATGCAACCAGAAAACATGGATTTATTGTATTCGAAATAAATCTGATGTATTTGATACTTTCAAGAAATGGAAAGCTTTGGTTGAAAATGAGACAGGAAAAAGGTTGAAGTGTCTCAGATCAGACAATGGAGGTGAATATTGCAGCAAAGAGTTTGATAGGTACTGTTCAGAGAATGGAATTCGTAGAGAGAAGACAGTTCTAGGAACACCACAAGAAAATGGTGTGTCAGAAAGGATGAACAGGACAATCATGGAGCGTGCAAGGTGCATGAGATTGCATGCAGGATTGCCTTTACAATTTTGGGTAGATGCTGTAGATACTGTTGTTTATTTAATAAACAGAGGGCCTTCAAGTTCCTTGTATGGTGGTGTTCCAGAGGAGGCATGGACAGGTAAAAAGGTAAACTACTCTTTTTTTAAAGCCTTTTGGTTGTGAGGCCTTTGTCCATATTGATAAAGAGAATAGAACAAAGCTTGAGGCAGAGTCTAAGAAATGTACCTTTATTGGATATGGTGTTAATGATTTTGGTTATCGTTTATATGATTATGAAAATCACAAAATCATTAGGAGCAGAGATGTAATATTCAATGAAAAGGTCTTGTATAAGAATCAGTTGCAGGAAAAGAAACAAGAAAAAGAAAACAAGGAATACGCAGTTCTTGATGAAATTATTGAAACAGTAAAGGTGCCTGA

Ace file of the UniGene sp_v2.0_unigene2146 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	GK15001 gene product from transcript GK15001-RA	-	-	47%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	32%
Blast2go	hypothetical protein VITISV_037436 [Vitis vinifera]	-	-	58%

Source	GOs	Term	Type	e value	Identity
Blast2go	binding	GO:0005488	Molecular Function	0.0	58%

Source	InterPros	Term	Type	Identity
Sma3	Peptidase, cysteine peptidase active site	IPR000169	-	-
Sma3	Peptidase S8/S53, subtilisin/kexin/sedolisin	IPR000209	-	-
Sma3	Enolase	IPR000941	-	-
Sma3	CCAAT-binding transcription factor, subunit B	IPR001289	-	-
Sma3	Ionotropic glutamate receptor	IPR001320	-	-
Sma3	Integrase, catalytic core	IPR001584	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	Dihydrofolate reductase domain	IPR001796	-	-
Sma3	F-box domain, cyclin-like	IPR001810	-	-
Sma3	Pleckstrin homology domain	IPR001849	-	-
Sma3	Zinc finger, CCHC-type	IPR001878	-	-
Sma3	Germin	IPR001929	-	-
Sma3	DNA methylase, N-6 adenine-specific, conserved site	IPR002052	-	-
Sma3	Short-chain dehydrogenase/reductase SDR	IPR002198	-	-
Sma3	AAA+ ATPase domain	IPR003593	-	-
Sma3	K+ potassium transporter	IPR003855	-	-
Sma3	Protein of unknown function DUF295	IPR005174	-	-
Sma3	Cupin 1	IPR006045	-	-
Sma3	Dihydrofolate reductase	IPR012259	-	-
Sma3		IPR013084	-	-
Sma3	Reverse transcriptase, RNA-dependent DNA polymerase	IPR013103	-	-
Sma3	ABC-2 type transporter	IPR013525	-	-
Sma3	RmlC-like jelly roll fold	IPR014710	-	-
Sma3	Germin, manganese binding site	IPR019780	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LKX7

Fln msg: Overlapping hits, possible frame ERROR between 1382 and 1373, query STOP codon too far from subject stop. Distance to subject end: 57 aas, putative chimeric sequence, W1: There is no M at the beginning, your sequence is shorter than subject: 353 - 407

Fln protein: NPWVLKEVRHVPDLKKNLISTGQLGSEGCVTTFRDKAWKVTKGALVIAKGEKVGTLYLCNGISNSVNALTSKGADVALWHHRLGHMIEKGMKILHSRNLLPGLKNVDLDFCENCIYGKQKRVRFLRVGKEKKSEKLELVHTDVWGPAQVSSLGGSRYYVTFIDDxxxxxWIYCIRNKSDVFDTFKKWKALVENETGKRLKCLRSDNGGEYCSKEFDRYCSENGIRREKTVLGTPQENGVSERMNRTIMERARCMRLHAGLPLQFWVDAVDTVVYLINRGPSSSLYGGVPEEAWTGKKVNYSFFKAFWL*GLCPY**RE*NKA*GRV*EMYLYWIWC**FWLSFI*L*KSQNH*

Protein Length: 354

Fln nts: CGTCGTGACCTTGGCTGTCACTCATCTCGATAGAAAGGCACTCAGAATAGTACAGTTGTGCCTTGCAGCGTCAGTCGCCTTCAACATTTCGAAGGAAACCACGACAGAGGGTTTGATGAAGGCGTTGTCGAAACTATATGAAAAACCCTCGGCCTCAAACAAGGTATTTCTTATGAAACGGTTATTTAATATGAAGATGTCGGAAGGTGGATCTGTTGCAGACCATTTAAATGATTTTAATACAGTTACAAGTCAGTTAAGTTCTGTAGGGGTAAACTTTGATGATGAAATTAGAGCTCTGTTATTCTTGTGCTCATTACCAGAAAGTTGGAATGGTTTGGTAATGGCCATTAGTAACTCCGTTTCTGGATCTAGTACTTTAAAGTTTGATGATGTTGTCGGTGCTCTCCTTAGCGAGGAAATGCGATGGAAAAGCTCTGGTGAGACATCGGGAAATGCTTTGAGTGCAGAGTCTAGAGGAAGAAAAATAGGAAAGAGGAAAGAGCTCAGGATATCGCAGTAAATCAAGGAAAGGCAGATCCAAGTCCAGATCGGGGATAGTGTGCTGGAAATGTGGAAAGAAAGGACACCTAAAGAAAGACTGTAGATCTCGGAAGGGAAAAGAAGGAGATGCACAACAAGAAAACAATCATGAAGCAAATGTGACAGGTGAGGTGTTACAAGATGCTTTGATTCTTTCTTTCGAAAATATTACTGATTCCTGGGTGGTAGATTCAGGGGCCTCATTCCATGCCACACCTGATAAGAAATACTTTCATGACTATGTTCAAGGAGATTTTGGACAAGTTCGTCTGGGTGATGATAAACCCTGTAAAATTGTTGGAATGGGTAAGGTTCTTGTAAAACAGCAGAATTGAAACCCATGGGTACTAAAGGAGGTAAGGCATGTTCCGGATCTAAAGAAAAATCTGATTTCAACAGGGCAGTTAGGCAGTGAAGGTTGTGTGACAACTTTCAGAGATAAAGCTTGGAAGGTCACCAAAGGAGCCCTGGTAATAGCAAAAGGTGAAAAGGTTGGTACATTGTATTTATGTAATGGTATTTCTAACTCTGTTAATGCTTTGACTTCCAAAGGAGCAGATGTAGCATTATGGCATCATAGGCTCGGACACATGATTGAAAAAGGGATGAAGATTCTTCACTCAAGGAATTTGTTGCCAGGTTTGAAGAATGTTGATTTGGATTTCTGTGAGAACTGCATTTATGGAAAACAGAAGAGAGTCAGATTTCTTAGAGTTGGGAAAGAAAAGAAGAGCGAAAAATTAGAGCTTGTGCATACAGATGTATGGGGACCTGCTCAGGTATCATCTCTCGGTGGCTCTCGTTATTATGTTACTTTTATTGATGATxxxxxxxxxxxxxxxTGGATTTATTGTATTCGAAATAAATCTGATGTATTTGATACTTTCAAGAAATGGAAAGCTTTGGTTGAAAATGAGACAGGAAAAAGGTTGAAGTGTCTCAGATCAGACAATGGAGGTGAATATTGCAGCAAAGAGTTTGATAGGTACTGTTCAGAGAATGGAATTCGTAGAGAGAAGACAGTTCTAGGAACACCACAAGAAAATGGTGTGTCAGAAAGGATGAACAGGACAATCATGGAGCGTGCAAGGTGCATGAGATTGCATGCAGGATTGCCTTTACAATTTTGGGTAGATGCTGTAGATACTGTTGTTTATTTAATAAACAGAGGGCCTTCAAGTTCCTTGTATGGTGGTGTTCCAGAGGAGGCATGGACAGGTAAAAAGGTAAACTACTCTTTTTTTAAAGCCTTTTGGTTGTGAGGCCTTTGTCCATATTGATAAAGAGAATAGAACAAAGCTTGAGGCAGAGTCTAAGAAATGTACCTTTATTGGATATGGTGTTAATGATTTTGGTTATCGTTTATATGATTATGAAAATCACAAAATCATTAG___GAGCAGAGATGTAATATTCAATGAAAAGGTCTTGTATAAGAATCAGTTGCAGGAAAAGAAACAAGAAAAAGAAAACAAGGAATACGCAGTTCTTGATGAAATTATTGAAACAGTAAAGGTGCCTGA

Fln Alignment:

Contig2146___VPDLKKNLISTGQLGSEGC-VTTFRDKAW--KVTKGALVIAKGEKVGTLYLCNGISNSVNALTSKGADVA-LWHHRLGHMIEKGMKILHSRNLLPGLKNVDLD--FCENCIYGKQKRVRFLRVGKEKKSEKLELVHTDVWGPAQVSSLGGSRYYVTFIDDAxxxTWIYCIRNKSDVFDTFKKWKALVENETGKRLKCLRSDNGGEYCSKEFDRYCSENGIRREKTVLGTPQENGVSERMNRTIMERARCMRLHAGLPLQFWVDAVDTVVYLINRGPSSSLYGGVPEEAWTGKKVNYSFFKAF
B8LKX7______________VPNLKSNLLSVNQIANQGYKVEFYKDKCLIKDINSNYKVVASGYVENGLYKFGRFTSNEKALVAEVDNVSRLWHERMGHLNYKSLSLMKRFEMVYGLPKISQSQGVCEGCMSGKQHMEKFIKGKSWRAKTPLHLVHSDLMGPLEHPSISGSRYVLTFIDDYxxxIWVYFLKNKDEVFEKFKEFKAFVEKQSGKSIKILRTDNGKEYVNKEFDHYCKYNGIKREHTVPYTPQQNGVAERKNRTLMEMARCMLHARNMDPKFWAEAINTATYIVNRTPTIAVKHKTPEEAWSRRKPTVSHFKVF

SNPs (tot: 206)

Position	A %	C %	G %	T %	Probability
83		75		25	0.996473
109		25		75	0.996473
123		80		20	0.995493
161	71		28		1.0
162		85		14	0.993533
209	12			87	0.992554
212		87		12	0.992554
214		12	12	75	0.975346
216			87	12	0.992554
232		12		87	0.992554
233	87	12			0.992554
234		12		75	0.975346
235	12			87	0.992554
236		12		87	0.992554
244		85		14	0.993533
248	85		14		0.993533
257			85	14	0.993533
260		85		14	0.993533
274			75	25	0.996473
284	33			66	1.0
292	50		50		1.0
317		14		85	0.993533
328		85		14	0.993533
337	14	85			0.993533
338		85		14	0.993533
340	85		14		0.993533
352	14		85		0.993533
353		85		14	0.993533
359	14		85		0.993533
367	14		85		0.993533
373		83		16	0.994512
375	66		33		1.0
376		83		16	0.994512
378	16		83		0.994512
385		83		16	0.994512
387	16		83		0.994512
393	50		50		1.0
401		40		60	1.0
403	10		90		0.90298
422	20	80			0.999996
429	77		22		0.999999
436		11		88	0.923564
437		11		88	0.923564
438		11	88		0.923564
439	11	88			0.923564
448			12	87	0.992554
455		12		87	0.992554
458	12		87		0.992554
460		12		87	0.992554
474	85		14		0.993533
479	85		14		0.993533
496		14		85	0.993533
498	14		85		0.993533
499		14	85		0.993533
504			85	14	0.993533
506		85		14	0.993533
514	11			88	0.923564
515	44	11	44		1.0
516		87		12	0.992554
518	87			12	0.992554
526			75	25	1.0
530	12			87	0.992554
531	12		75	12	0.975346
534		87		12	0.992554
536	37		50	12	1.0
539		87		12	0.992554
540	12	87			0.992554
541			12	87	0.992554
550	12	75		12	0.975346
551			12	87	0.992554
554			87	12	0.992554
557	12		87		0.992554
560	87			12	0.992554
575	25	12	62		1.0
577	12	87			0.992554
581		12		87	0.992554
584	62		37		1.0
589	85	14			0.993533
591	14			85	0.993533
593		83		16	0.994512
596		16		83	0.994512
597		16		83	0.994512
599		83		16	0.994512
604	16	83			0.994512
606	16		83		0.994512
612	83			16	0.994512
614		83		16	0.994512
615		16		83	0.994512
620	25	75			0.996473
630		75		25	0.996473
638	33		66		0.997452
647	66	33			0.997452
657	33		66		0.997452
752	66		33		0.997452
753	33		66		1.0
768	33		66		0.997452
790		66		33	0.997452
791	33		66		0.997452
792		33		66	1.0
815	33		66		0.997452
818	66		33		0.997452
829		33		66	0.997452
831	33			66	0.997452
1040	66		33		0.997452
1047			66	33	0.997452
1081	40	60			1.0
1087		80		20	0.995493
1098		20	80		0.995493
1115	25		75		0.996473
1138			75	25	0.996473
1150	25		75		0.996473
1155	20		80		0.995493
1177	20		80		0.995493
1191		80		20	0.995493
1198	40		60		1.0
1199		60		40	1.0
1221	80		20		0.995493
1228		80		20	0.995493
1238	20		80		0.995493
1240			80	20	0.995493
1243			20	80	0.995493
1244	80		20		0.995493
1255	20		80		0.995493
1262			20	80	0.995493
1273	12	87			0.992554
1283	87		12		0.992554
1291	37		62		1.0
1294	87			12	0.992554
1295		12	87		0.992554
1298			12	87	0.992554
1304	62		12	25	1.0
1306		87		12	0.992554
1307	87	12			0.992554
1316	87		12		0.992554
1325	14		71		0.984337
1326	14		14		0.984337
1335	85	14			0.993533
1336		14		85	0.993533
1348	20		80		0.995493
1353		80		20	0.995493
1385		20		80	0.995493
1397		20		80	0.995493
1401	20	80			0.995493
1422		25		75	0.996473
1434	25		75		0.996473
1440		66	33		0.997452
1561		33		66	0.997452
1562		33	66		0.997452
1564			66	33	0.997452
1618		75		25	0.996473
1619	25	75			0.996473
1629		75		25	0.996473
1640		75		25	0.996473
1641		50		50	1.0
1644		50		50	1.0
1670	25		75		0.996473
1672	25	50		25	0.995533
1695	25	75			0.996473
1708		75		25	0.996473
1754		25		75	0.996473
1780		28		71	1.0
1786	14	85			0.993533
1787	85			14	0.993533
1793			83	16	0.994512
1799	16	66		16	0.988813
1804		83		16	0.994512
1807	83		16		0.994512
1808	83		16		0.994512
1809		66	16	16	0.988813
1851	11		88		0.923564
1855		88		11	0.923564
1856	88		11		0.923564
1857			88	11	0.923564
1867		88		11	0.923564
1868		77		22	0.999999
1869		11	88		0.923564
1889		87		12	0.992554
1890		62	12	25	1.0
1891			87	12	0.992554
1908			22	77	0.999999
1914			87	12	0.992554
1918		87		12	0.992554
1920	12		87		0.992554
1941	25		75		1.0
1942	75			25	1.0
1945	75		25		1.0
1947			87	12	0.992554
1948	12	87			0.992554
1949		87	12		0.992554
1951	14	28		57	1.0
1952	28	71			1.0
1954		14		85	0.993533
1958	85		14		0.993533
1959		85	14		0.993533
1960		85		14	0.993533
1962	14		14	71	0.984337
1966	14			85	0.993533
1967		85		14	0.993533
1968	42		42	14	1.0
1970			66	33	1.0
1985	16		83		0.994512
1993		60		40	1.0
1994	20		80		0.995493
1997	20	40		40	1.0
1998	20		80		0.995493
2003	50			50	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain