SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene2097

Length: 2125 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene2097
CGTCGTGACCTTGGCTGTCACTCAAATTCTTCTTTTGTTCTGAATATAGTGAACAAGAGTGGAAACGGTGGCGATGGCTTGGCTTTCATTGTAACGAATGAGAAAAATGGTTCCCCTGAGAATAGTTACGGGCAGTATCTGGGACTGTTCAACTCTACCACGAATGGGGATTCAAATAACAGATTGGTTGCAGTGGAGTTCGATACCAGAAAGAGCAACGCCAACGACACAGATGATAATCACGTCGGAATAGATGTGAACAAGATAGAATCCATCAAAAGTGCTTCTGTGAGTCCTCGCAATATTACCCTTCTAAGCCAATCGGACATTGCTGCGCTGGTTCAGTACGACGGGGGTTCGAAACTGTTGAGCGTGTATATCAACAATGGTGACACAGCGAATCCCTCAAACCCTCTTCTTTCTGTTAAACTTGATTTGTCTATGATTCTGGATGATGAAATCTATTTTGGCTTCTCGGCTTCGACGGGTGTTCAAACCGAGCTTAACTGCATTCGATCCTGGAACCTCACTATGCAAAATCTGGGGTCAGGGAACCGTAATGGGCTCTATTCGTGTTCTGGACAGAGCCCTGACGAATTCGACAGTGGGACCTCGTAAGTTCAGACTGAGGGAAGTTAGGCGTGCTACTCGGAATTTTGATAGGAAAAGCCTGCTGGGTCAAGGAGGGTTTGGGGCTGTGTACAGAGGGGTGCTGTCTAATAAAACCCCTGTCGCTGTAAAACGAGTTTCGGAGAACTCTAATCAGGGTGAGCAGGAGTTCATGGCAGAGGTCATGATTATTAGTAGGCTTCGCCACAAGAACCTTGTGAGGCTGTTAGGTTGGTGCCATGAAGATAACGAACTGCTGCTGATCTATGACTTGATGCCCAATGGTAGCCTGGATAAGTTGATTTTTGAAGAAGATGCTAAGCACGAGGTTTTGGACTGGCATCACAGATGCAAGATAGTGTGTGGAGTTGCCTCGGCTATGCTCTATCTGCACGAAGAATGTGAGCAGCAGGTGATCCACAGGGATTTGAAGGCTAGCAACATCATGCTTGATTCTGATTACAATGCTCGTCTGGGTGATTTTGGGCTTGCTAGATTGATTGAACTTAATAAGAACTCTTATACCACAACTGCAATAGCAGGTACTCCCGGGTATATTGCACCAGAGTACTTTCACACCGGGAGGTCTACAACTGAATCTGATGTGTTTGGTTTTGGGGCTGTGGCTCTAGAGGTTGCTTGTGGGAGAAGACCCAGAACAGAGGCACAGCTTTATCTTGTTGATTGGGTCTGGGGGCTGTATCGAGAAGGCAGGCTTCTTGATGCTGCAGATCCCAGGCTAAATGAGTTTGATAAAGATGAGATGATATGCATGTTACTTGTTGGTATGGCTTGCTCTCATCCCAACCCTCAAGAAAGGCCAACAATGAGACAGGTTCTACAAATCCTTACCAAAGATGCACCCCCTCCACTTATGCCCTTGTTTAAGCCTGCATTTGTCTGGCCTGCCCCCGGTATGACTGATATCCAATCTAGTTCTAGTGATTTTAGCACACAGTAAAACAGAGCAGCTTTGAATGTGTAGATAAGAAGATCAAGGGAAGTGAGCAGATTTGGGGCAATCCTCTCTTCCCCTCAAATTTCCTTTTTCCTTGTGTTTGGAACATTGTTTTGGTTGAAGAGGATGTGGAGCAGGGATGGGTATTTTCAATTATTTTAGGAATTTCGTTTTTTCCCCTTTTGTGTGTGTGTATTGCCATGCTCGTTCAAGAAATCAATCAGGTGTCCTTTTGTAGTTTGTCTTGTTCTTTTGCTTTTACTCGAGGAAGTGGATCAAGTACCTGCTCGTAGAAATGTGGCCTTGCCACATGGGTAGATAAAGTCAAAGGCCTCGCCCTGTGCTAAAATCTCTTCTCCGTTATACTATCCCATATGACATGTAAAAGCAACAGCGGAAATCCAGACTTTATTATGTATGCTGGACTTTTCTGAAGGATGTAAGCCACTGAATGGTTATTTCGTGCTAGTTTATCTCAGAAATCATTTGCCTGTGTAAATATCTTTTATCATATGTGAGTTAAGGGAATGTGTTGAGAGAAGCCATAAAAATAAGTTT

Ace file of the UniGene sp_v2.0_unigene2097 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Probable L-type lectin-domain containing receptor kinase S.5 n=2 Tax=Arabidopsis RepID=LRKS5_ARATH	-	-	51%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	37%
Blast2go	probable l-type lectin-domain containing receptor kinase -like	-	-	70%

Source	GOs	Term	Type	Identity
Blast2go	phosphorylation	GO:0016310	Biological Process	70%
Blast2go	carbohydrate binding	GO:0030246	Molecular Function	70%
Blast2go	protein kinase activity	GO:0004672	Molecular Function	70%
Blast2go	nucleotide binding	GO:0000166	Molecular Function	70%

Source	InterPros	Term	Type	Identity
Sma3	ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain	IPR000194	-	-
Sma3	Protease inhibitor I4, serpin	IPR000215	-	-
Sma3	Reverse transcriptase	IPR000477	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Chromo domain/shadow	IPR000953	-	-
Sma3	Legume lectin, alpha chain, conserved site	IPR000985	-	-
Sma3	Legume lectin domain	IPR001220	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Integrase, catalytic core	IPR001584	-	-
Sma3		IPR001899	-	-
Sma3	Peptidase aspartic, active site	IPR001969	-	-
Sma3	Short-chain dehydrogenase/reductase SDR	IPR002198	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	Glucose/ribitol dehydrogenase	IPR002347	-	-
Sma3	Ribosomal protein L10, eubacterial, conserved site	IPR002363	-	-
Sma3	Gnk2-homologous domain	IPR002902	-	-
Sma3	Plastocyanin-like	IPR003245	-	-
Sma3	Epidermal growth factor-like	IPR006210	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Cupredoxin	IPR008972	-	-
Sma3	Retroviral aspartyl protease	IPR013242	-	-
Sma3	Concanavalin A-like lectin/glucanase, subgroup	IPR013320	-	-
Sma3	SKG6/AXL2 alpha-helix transmembrane domain	IPR014805	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NVC0

Fln msg: Separated hits, possible frame ERROR between 537 and 611, your sequence is shorter than subject: 524 - 636

Fln protein: RRDLGCHSNSSFVLNIVNKSGNGGDGLAFIVTNEKNGSPENSYGQYLGLFNSTTNGDSNNRLVAVEFDTRKSNANDTDDNHVGIDVNKIESIKSASVSPRNITLLSQSDIAALVQYDGGSKLLSVYINNGDTANPSNPLLSVKLDLSMILDDEIYFGFSASTGVQTELNCIRSWNLTMxxxxxxxxxxxxxxxxxxxxxxxxxxPRKFRLREVRRATRNFDRKSLLGQGGFGAVYRGVLSNKTPVAVKRVSENSNQGEQEFMAEVMIISRLRHKNLVRLLGWCHEDNELLLIYDLMPNGSLDKLIFEEDAKHEVLDWHHRCKIVCGVASAMLYLHEECEQQVIHRDLKASNIMLDSDYNARLGDFGLARLIELNKNSYTTTAIAGTPGYIAPEYFHTGRSTTESDVFGFGAVALEVACGRRPRTEAQLYLVDWVWGLYREGRLLDAADPRLNEFDKDEMICMLLVGMACSHPNPQERPTMRQVLQILTKDAPPPLMPLFKPAFVWPAPGMTDIQSSSSDFSTQ*

Protein Length: 525

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAATTCTTCTTTTGTTCTGAATATAGTGAACAAGAGTGGAAACGGTGGCGATGGCTTGGCTTTCATTGTAACGAATGAGAAAAATGGTTCCCCTGAGAATAGTTACGGGCAGTATCTGGGACTGTTCAACTCTACCACGAATGGGGATTCAAATAACAGATTGGTTGCAGTGGAGTTCGATACCAGAAAGAGCAACGCCAACGACACAGATGATAATCACGTCGGAATAGATGTGAACAAGATAGAATCCATCAAAAGTGCTTCTGTGAGTCCTCGCAATATTACCCTTCTAAGCCAATCGGACATTGCTGCGCTGGTTCAGTACGACGGGGGTTCGAAACTGTTGAGCGTGTATATCAACAATGGTGACACAGCGAATCCCTCAAACCCTCTTCTTTCTGTTAAACTTGATTTGTCTATGATTCTGGATGATGAAATCTATTTTGGCTTCTCGGCTTCGACGGGTGTTCAAACCGAGCTTAACTGCATTCGATCCTGGAACCTCACTATGCAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxACCTCGTAAGTTCAGACTGAGGGAAGTTAGGCGTGCTACTCGGAATTTTGATAGGAAAAGCCTGCTGGGTCAAGGAGGGTTTGGGGCTGTGTACAGAGGGGTGCTGTCTAATAAAACCCCTGTCGCTGTAAAACGAGTTTCGGAGAACTCTAATCAGGGTGAGCAGGAGTTCATGGCAGAGGTCATGATTATTAGTAGGCTTCGCCACAAGAACCTTGTGAGGCTGTTAGGTTGGTGCCATGAAGATAACGAACTGCTGCTGATCTATGACTTGATGCCCAATGGTAGCCTGGATAAGTTGATTTTTGAAGAAGATGCTAAGCACGAGGTTTTGGACTGGCATCACAGATGCAAGATAGTGTGTGGAGTTGCCTCGGCTATGCTCTATCTGCACGAAGAATGTGAGCAGCAGGTGATCCACAGGGATTTGAAGGCTAGCAACATCATGCTTGATTCTGATTACAATGCTCGTCTGGGTGATTTTGGGCTTGCTAGATTGATTGAACTTAATAAGAACTCTTATACCACAACTGCAATAGCAGGTACTCCCGGGTATATTGCACCAGAGTACTTTCACACCGGGAGGTCTACAACTGAATCTGATGTGTTTGGTTTTGGGGCTGTGGCTCTAGAGGTTGCTTGTGGGAGAAGACCCAGAACAGAGGCACAGCTTTATCTTGTTGATTGGGTCTGGGGGCTGTATCGAGAAGGCAGGCTTCTTGATGCTGCAGATCCCAGGCTAAATGAGTTTGATAAAGATGAGATGATATGCATGTTACTTGTTGGTATGGCTTGCTCTCATCCCAACCCTCAAGAAAGGCCAACAATGAGACAGGTTCTACAAATCCTTACCAAAGATGCACCCCCTCCACTTATGCCCTTGTTTAAGCCTGCATTTGTCTGGCCTGCCCCCGGTATGACTGATATCCAATCTAGTTCTAGTGATTTTAGCACACAGTAAAACAGAGCAGCTTTGAATGTGTAGATAAGAAGATCAAGGGAAGTGAGCAGATTTGGGGCAATCCTCTCTTCCCCTCAAATTTCCTTTTTCCTTGTGTTTGGAACATTGTTTTGGTTGAAGAGGATGTGGAGCAGGGATGGGTATTTTCAATTATTTTAGGAATTTCGTTTTTTCCCCTTTTGTGTGTGTGTATTGCCATGCTCGTTCAAGAAATCAATCAGGTGTCCTTTTGTAGTTTGTCTTGTTCTTTTGCTTTTACTCGAGGAAGTGGATCAAGTACCTGCTCGTAGAAATGTGGCCTTGCCACATGGGTAGATAAAGTCAAAGGCCTCGCCCTGTGCTAAAATCTCTTCTCCGTTATACTATCCCATATGACATGTAAAAGCAACAGCGGAAATCCAGACTTTATTATGTATGCTGGACTTTTCTGAAGGATGTAAGCCACTGAATGGTTATTTCGTGCTAGTTTATCTCAGAAATCATTTGCCTGTGTAAATATCTTTTATCATATGTGAGTTAAGGGAATGTGTTGAGAGAAGCCATAAAAATAAGTTT

Fln Alignment:

Contig2097___NSSFVLNIVNKSGN-GGDGLAFIVTNEKNGSPENSYGQYLGLFNSTTNGDSNNRLVAVEFDTRKS-NANDTDDNHVGIDVNKIESIKSASVSPRN------ITLLSQSDIAALVQYDGGSKLLSVYINNGDTANPSNPLLSVKLDLSMILDDEIYFGFSASTGVQTELNCIRSWNLTMQxxxxxxxxxxxxxxxxxxxxxxxxxPRKFRLREVRRATRNFDRKSLLGQGGFGAVYRGVL-SNKTPVAVKRVSENSNQGEQEFMAEVMIISRLRHKNLVRLLGWCHEDNELLLIYDLMPNGSLDKLIFEEDAKHEVLDWHHRCKIVCGVASAMLYLHEECEQQVIHRDLKASNIMLDSDYNARLGDFGLARLIELNKNSYTTTAIAGTPGYIAPEYFHTGRSTTESDVFGFGAVALEVACGRRP----RTEAQLYLVDWVWGLYREGRLLDAADPRL---NEFDKDEMICMLLVGMACSHPNPQERPTMRQVLQILTKDAPPP
A9NVC0______________STTFVFSIVPPPSNAGGNGIAFFMTPHTS-SMDAQPSQYLGLLNLTTNGQAYNHLFAVEFDTIMNVEFNDPDGNHVGVDVNNLVSVQTETAGYWNGEEFHELNLRSGRNIQAWIDYDHLQSRLDVTMTVVGLPRPQKPLISLQIDLHNVLQEKMYVGFSAATGLFMEDHYVLAWSFTTQxxxxxxxxxxxxxxxxxxxxxxxxxPHRFTYKELSIATSRFRDENVLGYGGFGMVYKGVLPSSGQEVAVKCITTEFTEGMKGFVAEISSMGRLRHRNLVQLRGWCRRHTQLFIVYDYMPNGSLDKLIFGNPTT--VLPWHRRYAILKGVAAGLLYLHEQWEKRVVHRDIKSSNVLLDSELNGRLGDFGLARLYDHAENP-ETTHVVGTLGYIAPELIQTGKATPSSDVFSFGVLLLEVACGRNPVDSLEDSERMILAEWAWELYTEGRLLEASDPKLAGKGGYDVGEMEKVLKLGLLCSHPEPESRLGMRHVCQILDGEAPIP

SNPs (tot: 19)

Position	A %	C %	G %	T %	Probability
1032	12		87		0.992554
1055	12			87	0.992554
1056		12		87	0.992554
1057	12		87		0.992554
1059	85			14	0.993533
1090		83		16	0.994512
1143	16	83			0.994512
1145	16		83		0.994512
1146	83			16	0.994512
1147			83	16	0.994512
1243	20	80			0.995493
1246		80	20		0.995493
1247	80			20	0.995493
1309	25	25			0.995533
1418	20		80		0.995493
1509			75	25	0.996473
1510	75		25		0.996473
1512		75	25		0.996473
1514			25	75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain