SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1920

Length: 2228 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene1920
TGATCCCGCTGAACAACCTGATCAGCGCCCCATAAGGAGAGGAGTGGCTAGCCAGTTTATGAAGTTGGAAGATATGGGCCGTCGTGACCTTGGCTGTCACTCATTATGTCTTAAGAATATACAGCCGCGTGAATGATGCCAAGACAGGTGAAATTGCTTGTCGTACAGCAGGGGAAGTAAAGAAATGGATGGAAGCTTTTGAGCATGCTATTCAAGAGGCTTCCTATGATTTTGGAAGGCATAGCTCCCGGAGAATAATAAACAATGAAGATGAGTTTGGGCTTGGGCATAGGCCCCGTGTTAGAAGATATGCACATGGACTAAGAAAACGCATTACTATTGGAAAAGGTCCAGAGATGCTTCTGAGGAAGACTTCCAACGTTAGAAAGGATTCAGACTTCGGTAGTTTTGACGACACTGAAGGGGATGTGATGGAAAAACATGAATGGAAGTGTATTCATACTGTTAATGGTATACGAATCCTTGAGGATGTGGCTAATCACAAGGGGGATAAAGTTCTCCTCATGAAGTCTGTTGGCATTGTAGATGCTAGTCCAGATGCTGTGTTTGAGATGGTCTTCAGTATTGACAAGTCAAAGAGATATGAATGGGATATATTGACTAGTGACCTAGAGCTGATTGAACGATTGGATGGACATAGAGATATTGTTTATGGAGCGTATGATCCAAAGTATTTGACAAGGTGGCACTCAAAAAGAGATTTTTTGCTTACTCGACACTGGCAACGTGCTCAAGATGGCACATATACTATTTTGCAATATCCAACTATGGTCAAGCCGTGGCCTGTAAAGTCTGGTTATCGCCATACTAAGCTAAATCCTACTGTTTGGGAAATCAGGAAGGCAAATCCGAGGGAAGCAAAAGGAGCTCAGAGTTCTTTTGTGACTCAAACAATGGAGATACATTCTTCAGGTTGGGGACGCTGGAAGAAGAGATATTATGCCAAATTTGAAAAGACTGTTCCATATATCCTACTCTGTCAAATAGCAGGTTTAAGAGAATACTTTGGAGCAAATCCTTCTTTCAGTCCTGAGCCATCAATAGGCACTGCCCATAAGAAAAATCAAAAGGTTTCAGATGTCAGTGGTGAATCTGAGGATTCCGAAACAAATGAGGACTTTTATGATGCTATTGCTGCTGAAAGTCCTATAGAAGATGAAGATAGTGATGAGGATGAAAATTCTCCTACCACGGATGTTGGGCTGAAGTTCAAAAATATTTCTTGGGCTCTTATGAGAATGCGGCCACCAGGTCGAGAGGCAAACACAGAATTAATTTTGAATGTTCCTGCTATTGATATAGACACAAATCAATTTAAAGGCTCCTTGCAGAATGGAAAAAGTAAGACTGATTCAAACTGCTGGGCTGATCCTGGTGGCACTGGTTTCATGGTCAGGAGCAAAACATATCTCAAGGATTACTCTAAGGTCCCAGGAGGAGATCCACTTTTGAAGCTCTTGGCAGTTGACTGGTTCAAGTCAAACAATAAAGTTCAGAAGGTTGCAGTGCATCCCCGGTGTGTTGTTCAGACAGAAGTAGGTAAAAGTTTGCCATTCCTTCTTGTCGTAAATTTGCAGGTACCTTCCAAACCAAACCACAGCATGGTCTTTTATTTTGGGGCCAACAGGCCAATCCGTAAAGGATCTTTGCTGGATAAGTTTGCAAATGGTGATGACATGTTTCGGGATACACGCTTTAAACTTATCCCAAGTATTAAAGAGGGATATTGGATGGTCAAGCGTGCTGTTGGCACTAAAGCTTGTCTCTTGGGAAAGGCAGTGACATGTGAATATCTACGACAAGATAATTTTTTAGAGATTGATGTTGATATTGCGTCTTGACCTTGGCTGTCACTCATTATTGGTCTTGTTCTTGGATACGTAACAAACCTTGTTGTTGACCTTGCAATTCTAATTGAGGCCAAGGAGGAGGATGAATTGCCTGAATATGTGCTGGGAACAGTTCGAGTAAATCATGTCAAACTTGAATCTGCTATACCATTCTGGGGAGATGTATGAAAGGATCCTAAAATCCGGTACCAAAGTTGTTCTTGGATGTAAAGTCCAGTGTTAATGCTCATCCTGCAGTAATGTATGGATGGGATATTCATGTCTTTGTATAGTTATTAAAGTCTTGCTCCATGTGAGAGGCAACAAGTTTTGAAAAAAAGATTGATTCTTGGTTCAGATAAAAGAATATTCTACATT

Ace file of the UniGene sp_v2.0_unigene1920 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Lipid binding protein, putative n=1 Tax=Ricinus communis RepID=B9SC17_RICCO	-	-	56%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	44%
Blast2go	uncharacterized protein	-	-	70%

Source	GOs	Term	Type	Identity
Blast2go	signal transduction	GO:0007165	Biological Process	70%
Blast2go	phosphatidylinositol binding	GO:0035091	Molecular Function	70%
Blast2go	plasma membrane	GO:0005886	Cellular Component	70%

Source	InterPros	Term	Type	Identity
Sma3	GPCR, rhodopsin-like, 7TM	IPR000276	-	-
Sma3	Pleckstrin homology domain	IPR001849	-	-
Sma3	Lipid-binding START	IPR002913	-	-
Sma3	2-oxo acid dehydrogenase, lipoyl-binding site	IPR003016	-	-
Sma3	Ribosomal protein S12/S23	IPR006032	-	-
Sma3	Domain of unknown function DUF1336	IPR009769	-	-
Sma3	Pleckstrin homology-like domain	IPR011993	-	-
Sma3	Reverse transcriptase, RNA-dependent DNA polymerase	IPR013103	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NVR7

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 56 aas, putative chimeric sequence, your sequence is longer than subject: 617 - 320

Fln protein: MEAFEHAIQEASYDFGRHSSRRIINNEDEFGLGHRPRVRRYAHGLRKRITIGKGPEMLLRKTSNVRKDSDFGSFDDTEGDVMEKHEWKCIHTVNGIRILEDVANHKGDKVLLMKSVGIVDASPDAVFEMVFSIDKSKRYEWDILTSDLELIERLDGHRDIVYGAYDPKYLTRWHSKRDFLLTRHWQRAQDGTYTILQYPTMVKPWPVKSGYRHTKLNPTVWEIRKANPREAKGAQSSFVTQTMEIHSSGWGRWKKRYYAKFEKTVPYILLCQIAGLREYFGANPSFSPEPSIGTAHKKNQKVSDVSGESEDSETNEDFYDAIAAESPIEDEDSDEDENSPTTDVGLKFKNISWALMRMRPPGREANTELILNVPAIDIDTNQFKGSLQNGKSKTDSNCWADPGGTGFMVRSKTYLKDYSKVPGGDPLLKLLAVDWFKSNNKVQKVAVHPRCVVQTEVGKSLPFLLVVNLQVPSKPNHSMVFYFGANRPIRKGSLLDKFANGDDMFRDTRFKLIPSIKEGYWMVKRAVGTKACLLGKAVTCEYLRQDNFLEIDVDIAS*PWLSLIIGLVLGYVTNLVVDLAILIEAKEEDELPEYVLGTVRVNHVKLESAIPFWGDV*

Protein Length: 618

Fln nts: TGATCCCGCTGAACAACCTGATCAGCGCCCCATAAGGAGAGGAGTGGCTAGCCAGTTTATGAAGTTGGAAGATATGGGCCGTCGTGACCTTGGCTGTCACTCATTATGTCTTAAGAATATACAGCCGCGTGAATGATGCCAAGACAGGTGAAATTGCTTGTCGTACAGCAGGGGAAGTAAAGAAATGG_-_ATGGAAGCTTTTGAGCATGCTATTCAAGAGGCTTCCTATGATTTTGGAAGGCATAGCTCCCGGAGAATAATAAACAATGAAGATGAGTTTGGGCTTGGGCATAGGCCCCGTGTTAGAAGATATGCACATGGACTAAGAAAACGCATTACTATTGGAAAAGGTCCAGAGATGCTTCTGAGGAAGACTTCCAACGTTAGAAAGGATTCAGACTTCGGTAGTTTTGACGACACTGAAGGGGATGTGATGGAAAAACATGAATGGAAGTGTATTCATACTGTTAATGGTATACGAATCCTTGAGGATGTGGCTAATCACAAGGGGGATAAAGTTCTCCTCATGAAGTCTGTTGGCATTGTAGATGCTAGTCCAGATGCTGTGTTTGAGATGGTCTTCAGTATTGACAAGTCAAAGAGATATGAATGGGATATATTGACTAGTGACCTAGAGCTGATTGAACGATTGGATGGACATAGAGATATTGTTTATGGAGCGTATGATCCAAAGTATTTGACAAGGTGGCACTCAAAAAGAGATTTTTTGCTTACTCGACACTGGCAACGTGCTCAAGATGGCACATATACTATTTTGCAATATCCAACTATGGTCAAGCCGTGGCCTGTAAAGTCTGGTTATCGCCATACTAAGCTAAATCCTACTGTTTGGGAAATCAGGAAGGCAAATCCGAGGGAAGCAAAAGGAGCTCAGAGTTCTTTTGTGACTCAAACAATGGAGATACATTCTTCAGGTTGGGGACGCTGGAAGAAGAGATATTATGCCAAATTTGAAAAGACTGTTCCATATATCCTACTCTGTCAAATAGCAGGTTTAAGAGAATACTTTGGAGCAAATCCTTCTTTCAGTCCTGAGCCATCAATAGGCACTGCCCATAAGAAAAATCAAAAGGTTTCAGATGTCAGTGGTGAATCTGAGGATTCCGAAACAAATGAGGACTTTTATGATGCTATTGCTGCTGAAAGTCCTATAGAAGATGAAGATAGTGATGAGGATGAAAATTCTCCTACCACGGATGTTGGGCTGAAGTTCAAAAATATTTCTTGGGCTCTTATGAGAATGCGGCCACCAGGTCGAGAGGCAAACACAGAATTAATTTTGAATGTTCCTGCTATTGATATAGACACAAATCAATTTAAAGGCTCCTTGCAGAATGGAAAAAGTAAGACTGATTCAAACTGCTGGGCTGATCCTGGTGGCACTGGTTTCATGGTCAGGAGCAAAACATATCTCAAGGATTACTCTAAGGTCCCAGGAGGAGATCCACTTTTGAAGCTCTTGGCAGTTGACTGGTTCAAGTCAAACAATAAAGTTCAGAAGGTTGCAGTGCATCCCCGGTGTGTTGTTCAGACAGAAGTAGGTAAAAGTTTGCCATTCCTTCTTGTCGTAAATTTGCAGGTACCTTCCAAACCAAACCACAGCATGGTCTTTTATTTTGGGGCCAACAGGCCAATCCGTAAAGGATCTTTGCTGGATAAGTTTGCAAATGGTGATGACATGTTTCGGGATACACGCTTTAAACTTATCCCAAGTATTAAAGAGGGATATTGGATGGTCAAGCGTGCTGTTGGCACTAAAGCTTGTCTCTTGGGAAAGGCAGTGACATGTGAATATCTACGACAAGATAATTTTTTAGAGATTGATGTTGATATTGCGTCTTGACCTTGGCTGTCACTCATTATTGGTCTTGTTCTTGGATACGTAACAAACCTTGTTGTTGACCTTGCAATTCTAATTGAGGCCAAGGAGGAGGATGAATTGCCTGAATATGTGCTGGGAACAGTTCGAGTAAATCATGTCAAACTTGAATCTGCTATACCATTCTGGGGAGATGTATGA___AAGGATCCTAAAATCCGGTACCAAAGTTGTTCTTGGATGTAAAGTCCAGTGTTAATGCTCATCCTGCAGTAATGTATGGATGGGATATTCATGTCTTTGTATAGTTATTAAAGTCTTGCTCCATGTGAGAGGCAACAAGTTTTGAAAAAAAGATTGATTCTTGGTTCAGATAAAAGAATATTCTACATT

Fln Alignment:

Contig1920___GSLQNGKSKTDSNCWADPGGTGFMVRSKTYLKDYSKVPGGDPLLKLLAVDWFKSNNKVQKVAVHPRCVVQTEVGKS-------LPFLLVVNLQVPSKPNHSMVFYFGANRPIRKGSLLDKFANGDDMFRDTRFKLIPSIKEGYWMVKRAVGT-KACLLGKAVTCEYLRQDNFLEIDVDIAS*PWLSLIIGLVLGYVTNLVVDLAILIEAKEEDELPEYVLGTVRVNHVKLESA
A9NVR7______________GSLQQVDPEDGINGWSSPPGNLFHVRGSNYFTKKQKVPAGDWLLKPLGMDWLKAGSKLDHVLGRPDNRVMAALSKANSDGKGLKTFVFAVNLQVPGREPHSAVFYYATDDPIPVGSLFYRFIHEDDHFRNSRFKIVNRIVKGPWIVKTAVGNYAACLLGKALRCNYIKGSNYLEIDVDIGSSALASAILHLALGYVNSVTVDMGFLVESQAEEELPERLLGAVRVSQMQMGSA

SNPs (tot: 35)

Position	A %	C %	G %	T %	Probability
1729	25		75		1.0
1736		25		62	1.0
1741		87		12	0.992554
1755	85		14		0.993533
1799	71	28			1.0
1800		28		71	1.0
1803		71		28	1.0
1830		85		14	0.993533
1831		14	85		0.993533
1859			90	10	0.90298
1954	27		72		1.0
1982		30	70		1.0
2008			30	70	1.0
2066			90	10	0.90298
2067			10	90	0.90298
2069		90	10		0.90298
2074	90		10		0.90298
2075		10	90		0.90298
2079			11	88	0.923564
2080			11	88	0.923564
2085		87	12		0.992554
2086	12			87	0.992554
2130	25			75	0.996473
2133			75	25	0.996473
2134		25		75	0.996473
2135		25	75		0.996473
2136	75	25			0.996473
2139		25	75		0.996473
2146		25	75		0.996473
2147		25	75		0.996473
2149		75		25	0.996473
2150	75	25			0.996473
2152			75	25	0.996473
2154	25	75			0.996473
2155			75	25	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain