SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1857

Length: 1639 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene1857 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	histidine amino acid transporter [Oryza sativa Indica Group]	-	-	64%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	29%
Blast2go	lysine histidine transporter 1-like	-	-	79%

Source	GOs	Term	Type	e value	Identity
Blast2go	integral to membrane	GO:0016021	Cellular Component	0.0	79%
Blast2go	plastid	GO:0009536	Cellular Component	0.0	79%

Source	InterPros	Term	Type	Identity
Sma3	Ribosome-binding factor A	IPR000238	-	-
Sma3	Peroxin-3	IPR006966	-	-
Sma3	Amino acid transporter, transmembrane	IPR013057	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5A7Z1

Fln msg: your sequence is shorter than subject: 446 - 508

Fln protein: GKNSLNVDSAPAQDYTDKDAERTRTADEFTSSRTANWWYSAIHNITAMVGAGVLSLPYTMVFLGWGPGILVHVLSWLVTLFTLWQLVEMHEIVPGKRFDRYHELAQHAFGEKVGLWILVPPNLMVEVGVNIVYMVTGGQSLKKFHETVCRSCKPLRQSYWILIFSSIHYLLALLPNLSSVAGVSMAAALMSLCYSTIAWVACVYRGRVEDVSYAYLHETPATTVFRVLNSLGVIGFAYAGHNVALEIQATIPSSPERPSKIPMWRGVMFAYLIVALCYFPVAIVGYWAFGQTVSDNLLLTLRKPDWLIAAANMMVGIHVIGSYQVFAMPVYDGLERVLVNKFKLPPGRSLRLVARYSYVAFTTFIAVTFPFFGDLLGFFGGLAFTPTSYFLPSMMWLAICKPKRFSLCWLLNWFCILFGFIIMVVSTIGGFRSLLTDAYTYRFYQ*

Protein Length: 447

Fln nts: GGCAAGAATTCTCTCAATGTTGATTCAGCACCTGCGCAGGATTATACAGATAAAGATGCTGAAAGGACGAGAACTGCAGATGAGTTTACATCATCGCGGACTGCAAACTGGTGGTACTCTGCAATCCATAATATCACTGCTATGGTTGGTGCTGGAGTGCTCAGTTTACCATATACCATGGTATTCTTAGGATGGGGTCCAGGTATTCTGGTACATGTGCTTTCTTGGTTGGTTACCTTATTTACACTCTGGCAGCTGGTAGAGATGCATGAAATCGTCCCAGGAAAGCGATTTGATCGATATCACGAGTTAGCACAGCATGCGTTTGGAGAGAAGGTTGGCCTATGGATTCTTGTTCCCCCGAATTTGATGGTTGAAGTTGGTGTGAACATAGTTTATATGGTTACTGGTGGACAATCATTAAAGAAATTTCACGAAACAGTGTGCAGAAGTTGTAAACCACTGAGGCAGTCTTACTGGATCCTCATTTTCAGTTCTATTCACTACCTTTTAGCACTGTTACCCAATCTGAGTTCTGTGGCAGGGGTCTCAATGGCTGCAGCACTTATGTCTCTATGCTACTCCACCATTGCTTGGGTAGCCTGCGTTTATCGTGGTAGAGTGGAGGATGTAAGCTATGCCTATTTACATGAAACACCAGCCACCACGGTGTTTCGTGTTCTTAATTCCTTGGGGGTAATTGGGTTTGCATATGCGGGACATAATGTAGCTCTGGAGATCCAAGCTACCATTCCTTCATCACCAGAAAGGCCATCTAAGATCCCCATGTGGAGGGGTGTTATGTTTGCATATCTTATTGTTGCTCTCTGTTACTTCCCAGTAGCTATAGTGGGTTACTGGGCCTTCGGTCAAACTGTTTCTGACAATTTGCTCTTGACACTCAGAAAACCAGATTGGCTCATTGCAGCTGCAAATATGATGGTGGGAATACATGTTATTGGAAGCTATCAGGTTTTTGCTATGCCAGTGTACGATGGGCTAGAAAGAGTGCTGGTAAACAAATTTAAGCTACCACCAGGACGTTCTCTTCGTTTAGTTGCTAGATATTCTTATGTTGCTTTCACCACTTTCATTGCTGTTACATTCCCTTTCTTTGGGGATTTGCTGGGTTTCTTTGGAGGCCTAGCGTTCACGCCAACTTCTTATTTTCTGCCTTCTATGATGTGGCTTGCCATCTGCAAGCCAAAAAGATTTAGCTTATGCTGGCTTCTGAATTGGTTTTGTATATTATTTGGCTTTATAATTATGGTGGTGTCAACAATTGGAGGATTCAGAAGCCTGCTCACTGATGCATATACTTATCGCTTCTACCAGTGA___ATGTGAATGGTGTTGTTAGGTGCAAGCAGTAGTCTCTACGAGACTTGTTGAAGTTGTTGAACAATGATGTAGGCTTTCTCTTGCGACCATTACTTGATTTGATTCAGAGCCATAATCCGTAGATTATATATCTACTTGTTATCATACCCTTCGTACTTCCTATCCCCGACCTACAAATGTAATCTAATTTACTAGGCTGAGAGTTACTCTTGAAATTGTGTTGTCAAAAAACCACGTAAATTTTGTAGAGAATATGCATTTGGAAGAGATGTTCCATTTCCCATCAAATATAAAAGGCTTG

Fln Alignment:

Contig1857___TDKDAERTRTADEFTSSRTANWWYSAIHNITAMVGAGVLSLPYTMVFLGWGPGILVHVLSWLVTLFTLWQLVEMH---EIVPGKRFDRYHELAQHAFGEKVGLWILVPPNLMVEVGVNIVYMVTGGQSLKK------FHETVCRSCKPLRQSYWILIFSSIHYLLALLPNLSSVAGVSMAAALMSLCYSTIAWVACVYRGRVEDVSYAYLH-------ETPATTVFRVLNSLGVIGFAYAGHNVALEIQATIPSSPERPSKIPMWRGVMFAYLIVALCYFPVAIVGYWAFGQTVSDNLL--LTLRKPDWLIAAANMMVGIHVIGSYQVFAMPVYDGLER----------VLVNKFKLP-PG------RSLRLVARYSYVAFTTFIAVTXXXXXXXXXXXXXXXFTPTSYFLPSMMWLAICKPKRFSLCWL-LNWFCILFGFIIM--VVSTIGGFRSLL
D5A7Z1______________TDEDGHPQRTGDVWTASS---------HVITAVIGSGVLSLAWSMSQLGWIAGPLVLLAFSFVTYYTSMLLADTYRSPDPVTGRRNYTYTDAVTAILGGK-RVFLCGIVQYLNLLGTTIGYTITASISMVAIGRSDCFHEKGRESPCHISNNLYMAIFGAAQVLLSQIPNFSKIWWLSTLAAVMSLTYSFIGLGLGIGMATEKGHSHGSLGGVGIAGVQKSVDKIWNIFQALGNIAFAYSFSMILVEIQDTVKSPPAENK--TMKKASFIGVVVTTMFYISVGCAGYAAFGDHAPGNLLTGFGFYNPFWLVDIANICIVIHLVGAYQVFCQPLYAFVEEWSANTWTKSCFIQNEYKVPIPGLGEFKLNLFRLVWRTCFVVFTTVVSMVLPFFNAIMGVLGAIAFFPLTVYFPIQMHIAQTKLRRWSFKWVALQLMCVLCFFVTMAALVGSIAGVVEVL

SNPs (tot: 14)

Position	A %	C %	G %	T %	Probability
418			77	22	0.999999
426		14		85	0.993533
427	85	14			0.993533
429	85			14	0.993533
841		33		66	0.997452
1313	33			66	0.997452
1314	33	66			0.997452
1316		66	33		0.997452
1317		66		33	0.997452
1364	16		83		0.994512
1365	20		80		0.995493
1366			80	20	0.995493
1367	80		20		0.995493
1368		80		20	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain