SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1822

Length: 2021 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene1822
CCCAGAAATATTCCATCACAGTTGTCTGGGTGTGGAAGGCGCCCTCACTACTATCATGTGCTTGTCTTTACGCTCAACCGATTCCGATTGCTCTAACGGCAGTCCCGACTTCTAGGTTGTATCTTTGGTTTTCTCACTCATCATTGGGGGACTAAAGGGCATTTAACAAGGCTGTGAAGGAGATAAGGAGTAAACAAAGGAGATGAGAGAGATTATAAATATTCATGTTGGGCAGGCTGGAATACAAGTGGGAAACACATGTTGGGAATTATTTTGTTTAGAGCATGGAATTCAACCAGATGGTTATCCCTCTAGTGATGGAATAATAAATGGAGGAACCTATGCGCATAGTGCCTTCTTCAGTGAAACCAGAGCTGGGAAGCATGTTCCCAGAGCTCTGTTTATTGATCTTGAACCAACAGTTATTGATGAGGTTAGGACAGGTCCATACAGGCAGCTTTTTCACCCTGAACAACTTATTTCTGGCAAGGAAGATGCAGCTAACAACTTTGCTCGGGGCCATTATACTGTGGGCAAAGATGTATTGGAGAATTGTCTAGACAGAATAAGGAAGCTCTGTGATAATTGCACCAGTTTGCAAGGATTTTTAGTTTTTAATGCCGTTGGAGGAGGTACTGGATCTGGTTTGGGATCCTTACTCTTGGAACGCCTTTCCGTTGAATATGGACGAAAACCAAAGCTGGGATTTAATATATACCCCTCTCCTCAGGTTTCCACTGCAGTTGTAGAACCATATAACAGTGTATTGTCAACTCATTCTCTCTTGGAGCACACAAATGTAGCAGTTGTTCTGGACAACGAAGCTATTTATGACATATGTAAACGATGTCTAGACATCGAAAGACCTACATACAAAAACTTGAACAGACTGATCTCGCAAGTTATATCGTCATTAACAAGTTCTCTGCGATTTCAAGGAGCCTTAAATGTAGACATAAATGAGTTTCAAACGAACCTTGTTCCCTTTCCTAGAATTCATTTTATGCTTTCATCTTATGCACCAGTAGTGTCTAAAGAGAAAGCCTATCATGAAGCTTTGTCAGTGAAAGAAATAACAAATTCTGTGTTCGAGGCCAGCAACATGATGGCTAAATGTGATCCCAGACACGGAAAATACATGGCCTGCTGTCTAATGTATAGAGGAGACGTGGTGCCCAAAGATGTGAATGCAGCAGTTTCAATGATCAAAACAAAACGAGCTGTACAATTCGTAGACTGGTGTCCGACAGGTTTCAAATGTGGAATAAACTATCAACCTCCCACAGTTGTGCCCGGAGGAGACTTATGTAAAGTCCCAAGAGCAGTGTGCATGATCAGCAACAACACAGCAGTTGCAGAGGTGTTCTCCCGTCTTGACCACAAATTCGATCTAATGTTTGCAAAGCGTGCATTTGTACACTGGTATGTTGGTGAAGGCATGGAAGAAGGTGAATTCTCAGAGGCCCGGGAAGACTTGGCTGCTCTTGAAAAAGACTACTACGAAGTGGCTGCAGATGATCTAGATGATGAAGAGGTGGATGAAGATGAAGTTTATTGAAGACCTTTCCAATATCTCGTTCTTTCTTTCCTCCAGTTTTATCTCTATACATTTTTATTATGTACATTTTGCATTCCTTATTAGATTCATTCCCGTCTCACTTATTTGAAATTTTCAGTGCTCTGCACCTAAAGCAGAATTCGCGAGTTTGTAGAATTGTCTGGATTTGTTGTAGCTGTAACATCAGGGATCACATTATGATCTAGCTACCTTTAGCAGAAATTTTGTTTTATAAGCAATGTCAAAACAAGAGCATCATATGTTGTATCTTTGTTCTGCACCAGAGTCAGCGAGTGTACTTTCTCCCAAATTATTTTAAATTCTAGTATTAATCTTCATCTTCTTATTGAGCACTTCTGTGAGTTGGATTTTCAACTTGTATATGAAGAGGCTCATGGGCTGATTAACTTGCTGTAAATTCTTGGCTGAACCATCTTACAGTAAACAATGTTGGAAATTCTAT

Ace file of the UniGene sp_v2.0_unigene1822 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Tubulin alpha-2/alpha-4 chain n=384 Tax=Eukaryota RepID=TBA2_ARATH	-	-	80%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	99%
Blast2go	alpha tubulin 1	-	-	92%

Source	GOs	Term	Type	Identity
Blast2go	protein polymerization	GO:0051258	Biological Process	92%
Blast2go	GTP catabolic process	GO:0006184	Biological Process	92%
Blast2go	microtubule-based movement	GO:0007018	Biological Process	92%
Blast2go	GTPase activity	GO:0003924	Molecular Function	92%
Blast2go	structural molecule activity	GO:0005198	Molecular Function	92%
Blast2go	GTP binding	GO:0005525	Molecular Function	92%
Blast2go	microtubule	GO:0005874	Cellular Component	92%
Blast2go	cytoplasm	GO:0005737	Cellular Component	92%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: C0PT17

Fln msg:

Fln protein: MREIINIHVGQAGIQVGNTCWELFCLEHGIQPDGYPSSDGIINGGTYAHSAFFSETRAGKHVPRALFIDLEPTVIDEVRTGPYRQLFHPEQLISGKEDAANNFARGHYTVGKDVLENCLDRIRKLCDNCTSLQGFLVFNAVGGGTGSGLGSLLLERLSVEYGRKPKLGFNIYPSPQVSTAVVEPYNSVLSTHSLLEHTNVAVVLDNEAIYDICKRCLDIERPTYKNLNRLISQVISSLTSSLRFQGALNVDINEFQTNLVPFPRIHFMLSSYAPVVSKEKAYHEALSVKEITNSVFEASNMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVSMIKTKRAVQFVDWCPTGFKCGINYQPPTVVPGGDLCKVPRAVCMISNNTAVAEVFSRLDHKFDLMFAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYYEVAADDLDDEEVDEDEVY*

Protein Length: 453

Fln nts: CCCAGAAATATTCCATCACAGTTGTCTGGGTGTGGAAGGCGCCCTCACTACTATCATGTGCTTGTCTTTACGCTCAACCGATTCCGATTGCTCTAACGGCAGTCCCGACTTCTAGGTTGTATCTTTGGTTTTCTCACTCATCATTGGGGGACTAAAGGGCATTTAACAAGGCTGTGAAGGAGATAAGGAGTAAACAAAGGAG_-_ATGAGAGAGATTATAAATATTCATGTTGGGCAGGCTGGAATACAAGTGGGAAACACATGTTGGGAATTATTTTGTTTAGAGCATGGAATTCAACCAGATGGTTATCCCTCTAGTGATGGAATAATAAATGGAGGAACCTATGCGCATAGTGCCTTCTTCAGTGAAACCAGAGCTGGGAAGCATGTTCCCAGAGCTCTGTTTATTGATCTTGAACCAACAGTTATTGATGAGGTTAGGACAGGTCCATACAGGCAGCTTTTTCACCCTGAACAACTTATTTCTGGCAAGGAAGATGCAGCTAACAACTTTGCTCGGGGCCATTATACTGTGGGCAAAGATGTATTGGAGAATTGTCTAGACAGAATAAGGAAGCTCTGTGATAATTGCACCAGTTTGCAAGGATTTTTAGTTTTTAATGCCGTTGGAGGAGGTACTGGATCTGGTTTGGGATCCTTACTCTTGGAACGCCTTTCCGTTGAATATGGACGAAAACCAAAGCTGGGATTTAATATATACCCCTCTCCTCAGGTTTCCACTGCAGTTGTAGAACCATATAACAGTGTATTGTCAACTCATTCTCTCTTGGAGCACACAAATGTAGCAGTTGTTCTGGACAACGAAGCTATTTATGACATATGTAAACGATGTCTAGACATCGAAAGACCTACATACAAAAACTTGAACAGACTGATCTCGCAAGTTATATCGTCATTAACAAGTTCTCTGCGATTTCAAGGAGCCTTAAATGTAGACATAAATGAGTTTCAAACGAACCTTGTTCCCTTTCCTAGAATTCATTTTATGCTTTCATCTTATGCACCAGTAGTGTCTAAAGAGAAAGCCTATCATGAAGCTTTGTCAGTGAAAGAAATAACAAATTCTGTGTTCGAGGCCAGCAACATGATGGCTAAATGTGATCCCAGACACGGAAAATACATGGCCTGCTGTCTAATGTATAGAGGAGACGTGGTGCCCAAAGATGTGAATGCAGCAGTTTCAATGATCAAAACAAAACGAGCTGTACAATTCGTAGACTGGTGTCCGACAGGTTTCAAATGTGGAATAAACTATCAACCTCCCACAGTTGTGCCCGGAGGAGACTTATGTAAAGTCCCAAGAGCAGTGTGCATGATCAGCAACAACACAGCAGTTGCAGAGGTGTTCTCCCGTCTTGACCACAAATTCGATCTAATGTTTGCAAAGCGTGCATTTGTACACTGGTATGTTGGTGAAGGCATGGAAGAAGGTGAATTCTCAGAGGCCCGGGAAGACTTGGCTGCTCTTGAAAAAGACTACTACGAAGTGGCTGCAGATGATCTAGATGATGAAGAGGTGGATGAAGATGAAGTTTATTGA___AGACCTTTCCAATATCTCGTTCTTTCTTTCCTCCAGTTTTATCTCTATACATTTTTATTATGTACATTTTGCATTCCTTATTAGATTCATTCCCGTCTCACTTATTTGAAATTTTCAGTGCTCTGCACCTAAAGCAGAATTCGCGAGTTTGTAGAATTGTCTGGATTTGTTGTAGCTGTAACATCAGGGATCACATTATGATCTAGCTACCTTTAGCAGAAATTTTGTTTTATAAGCAATGTCAAAACAAGAGCATCATATGTTGTATCTTTGTTCTGCACCAGAGTCAGCGAGTGTACTTTCTCCCAAATTATTTTAAATTCTAGTATTAATCTTCATCTTCTTATTGAGCACTTCTGTGAGTTGGATTTTCAACTTGTATATGAAGAGGCTCATGGGCTGATTAACTTGCTGTAAATTCTTGGCTGAACCATCTTACAGTAAACAATGTTGGAAATTCTAT

Fln Alignment:

Contig1822___MREIINIHVGQAGIQVGNTCWELFCLEHGIQPDGYPSSDGIINGGTYAHSAFFSETRAGKHVPRALFIDLEPTVIDEVRTGPYRQLFHPEQLISGKEDAANNFARGHYTVGKDVLENCLDRIRKLCDNCTSLQGFLVFNAVXXXXXXXXXXXXXXXXSVEYGRKPKLGFNIYPSPQVSTAVVEPYNSVLSTHSLLEHTNVAVVLDNEAIYDICKRCLDIERPTYKNLNRLISQVISSLTSSLRFQGALNVDINEFQTNLVPFPRIHFMLSSYAPVVSKEKAYHEALSVKEITNSVFEASNMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVSMIKTKRAVQFVDWCPTGFKCGINYQPPTVVPGGDLCKVPRAVCMISNNTAVAEVFSRLDHKFDLMFAKRAFVHWYVGEGMEEGEFSEAREDLAALEK
C0PT17______________MREIINIHVGQAGIQVGNTCWELFCLEHGIQPDGYPSSDGIINGGTYAHSAFFSETGAGKHVPRALFIDLEPTVIDEVRTGPYRQLFHPEQLISGKEDAANNFARGHYTVGKDVLENCLDRIRKLCDNCTSLQGFLVFNAVGGGTGSGLGSLLLERLSVEYGRKPKLGFNIYPSPQVSTAVVEPYNSVLSTHSLLEHTDVAVVLDNEAIYDICKRCLDIERPTYKNLNRLISQVISSLTSSLRFQGALNVDINEFQTNLVPFPRIHFMLSSYAPVVSKEKAYHEALSVKEITNSVFEASNMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVSMIKTKRAVQFVDWCPTGFKCGINYQPPAVVPGGDLCKVPRAVCMISNNTAVVEVFSRLDHKFDLMFAKRAFVHWYVGEGMEEGEFSEAREDLAALEK

SNPs (tot: 11)

Position	A %	C %	G %	T %	Probability
1800	50		50		1.0
1818		50		50	1.0
1831	40		60		1.0
1881	85		14		0.993533
1903			83	16	0.994512
1921	20		80		0.995493
1922	20		80		0.995493
1923	80		20		0.995493
1925		20		80	0.995493
1947			75	25	0.996473
1970			25	75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain