SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1804

Length: 2163 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene1804
ATGGGGATCTCATTCTTCTTCATGGGGATCGAGGGAAGTTTATTGTCGAAACAGTTGTTATAGCTTTGTTTGGGTGAGAAAACTATTATAATTCGTCAATGCAACAAAGACGAAATCCTAATTTGATATCACCGATATCAATCTAGCTCGTTTTCCTACAGATCTAGTCTGATCGGTACCGATACTGATTTCCCTGCAGATCTAGTCTCATCAGTTCTGAAATTTACTAATACGGAAAGTTAAGACGCCGTTTTATATTTTGGGTATATTGAAAGTAGTTTTCTGATCATAGATATGGCACTGAAGAAGGCGTCGAAGCCGGTTTTCCGACTCTTCTCAGCGGAATGCAATGCTCGGCGGACATTCTTTCGGTCGTTGGCGAATTCAGTGGATCAGGGTTTGCAGAGAGATTGCCTTCACCAATCAGGATCTTCGAAATCTCACTGGGGTTCGAATTCGGGGACTTATATGAGGTGGGCTTCGGCGCTCGTCCTCAGTGCTGGTTTGGGTTTACTGCAATTTCCGGAATTCAAAAGTGATTTTAGTAATTCAAACGAAGATCAACGAACGGAAGGACGCACAAACAAATTTATTCTTGGAGATTCATACAGGAGACGACTATTTTTCAACTACGAGAGGAGAATAAGGATGCGAAGTTCTCCTGAAAAGGTATTTGAATACTTTGCATCATTTAGAACTCCTGATGGAGAGTTATTTATGACTCCTGCTGACCTGGTGCGAGCAGTAGTTCCTGTATTTCCACCACCAGATTCTAATATTGTCAGGGATGGATCTTTGAGAGGGGAACGATCTCCTGGAGAACTTCATTGCCCTCAATCAGAGTTATTCATGCGGTTTGATACTAATGGGGATGGCCTTATCTCCTTTCCAGAGTATATTTTTTTCGTAACACTCTTGAGTATTCCTGAATCGGATATTTTAGCTACGATCAAAATGTTTGATCTGGACCGCAATGGGGAGATAAAGAAAGAAGAATTCAAGAAAGTAATGAAATTTTTGCGAGCTAATAACAGGCAAGGGTCTGCTCAACGTGATGGCCTTCGTATAGGACTTAAAGTCGACAAATCTGTGGATAATGGAGGTCTTGTGGAGTATTTTTTTGGTAAAGGACGGGAAGGACCGTCTTCAGCTTATGAACTTTGCACAGTTTGTCAAGGATTTGCACAAGGAAATTACACGTCTAGAGTTTTTGCACTATGATCATCAGGGATGTGGTAGCATATCAGCCAGAGATTTTGCGTTGTCCATGGTTGCTGCAGCAGATGTGATATGTGTAAATCGGTACTTGGATCGGGTAGATGATCTCAGCAAGGACCCCAGTCTGAGTAATATACACATTACACGTGAGGAATTTGAGAACTTTGCTGAGTTGCGCAAGAAACTGAAGCCTCTATCATTGGCCATATCCAGTTATGGAAAAGTTTATGGAATCTTGACCAAGTCTGATTTTCGACGAGCAGCATCTCATGTTTGTGACGTTGACGTCACAGGCAACATGGTTGATATCATATTTTATATTTTTGACAAAAATAACGATGGCAACCTTAGCACGGATGAATTTTTGGCAGTATTAGAGCGGCGTGAAAAAGATATTGCATACCCTATAGAGTCTGGGATTATTCACTCGCTGACTTCTTGGTGGAAATGTGCGAAAGATTGTCGTTCGGCATGGAATTATTAGTGATTTGAAGAACCATTCATTATTATCCCGTGCAAATCAAGGAATTTTACAATGAGGTTTAATGAGACTCCACAATTGCAGCAGGTGCAGGACTTGTGACAGCTACTTGTGTACATTGGAAGGATCAAGCAAATCATATTATCATTGGAAAATCCAGTCATGGTGTCTGGCAATCCAAGAAATTGCCTTCAAGCCCTAGTGACTTAGGCTGGAGGCAGAAAGACCAGCAATGGCTCTTTTCAAATGGTCTTTCGGGAAGTCTTCATTATGGCATCTAACAATTCACATGCTTTCACATTGCCAATTATTTAGAATGCCTAGTGGGTTTAATCGGAGTTCTTGGAGATGACTTGTCGAGTACTGGAAACTGTCTCATGTAAAATATCAATTTTTTGATGTCATCTTTAAACGTAAAAAAACTAATATGTGCTCGCTAGTTAAGAGTGTAACTTAATATCACA

Ace file of the UniGene sp_v2.0_unigene1804 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	EF-hand, calcium binding motif-containing protein [Arabidopsis thaliana] emb\|CAA16584.1\| putative protein [Arabidopsis thaliana] emb\|CAB79924.1\| putative protein [Arabidopsis thaliana] gb\|AAL59985.1\| unknown protein [Arabidopsis thaliana] gb\|AAM67515.1\| u	-	-	65%
FL-Next	tr=Os02g0613000 protein; Oryza sativa subsp. japonica (Rice).	-	-	72%
Blast2go	calcium uptake protein mitochondrial-like	-	-	79%

Source	GOs	Term	Type	Identity
Blast2go	calcium ion binding	GO:0005509	Molecular Function	79%
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	79%
Blast2go	mitochondrion	GO:0005739	Cellular Component	79%
Blast2go	plastid	GO:0009536	Cellular Component	79%

Source	InterPros	Term	Type	Identity
Sma3	S100/Calbindin-D9k, conserved site	IPR001751	-	-
Sma3	Calcium-binding EF-hand	IPR002048	-	-
Sma3	EF-hand-like domain	IPR011992	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	EF-hand	IPR018248	-	-
Sma3	EF-HAND 2	IPR018249	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: tr_plants

Fln subject: Q0DZK7

Fln msg: Overlapping hits, possible frame ERROR between 1128 and 1118,

Fln protein: MALKKASKPVFRLFSAECNARRTFFRSLANSVDQGLQRDCLHQSGSSKSHWGSNSGTYMRWASALVLSAGLGLLQFPEFKSDFSNSNEDQRTEGRTNKFILGDSYRRRLFFNYERRIRMRSSPEKVFEYFASFRTPDGELFMTPADLVRAVVPVFPPPDSNIVRDGSLRGERSPGELHCPQSELFMRFDTNGDGLISFPEYIFFVTLLSIPESDILATIKMFDLDRNGEIKKEEFKKVMKFLRANNRQGSAQRDGLRIGLKVDKSVDNGGLVEYxxxxxDGKDRLQLMNFAQFVKDLHKEITRLEFLHYDHQGCGSISARDFALSMVAAADVICVNRYLDRVDDLSKDPSLSNIHITREEFENFAELRKKLKPLSLAISSYGKVYGILTKSDFRRAASHVCDVDVTGNMVDIIFYIFDKNNDGNLSTDEFLAVLERREKDIAYPIESGIIHSLTSWWKCAKDCRSAWNY*

Protein Length: 471

Fln nts: ATGGGGATCTCATTCTTCTTCATGGGGATCGAGGGAAGTTTATTGTCGAAACAGTTGTTATAGCTTTGTTTGGGTGAGAAAACTATTATAATTCGTCAATGCAACAAAGACGAAATCCTAATTTGATATCACCGATATCAATCTAGCTCGTTTTCCTACAGATCTAGTCTGATCGGTACCGATACTGATTTCCCTGCAGATCTAGTCTCATCAGTTCTGAAATTTACTAATACGGAAAGTTAAGACGCCGTTTTATATTTTGGGTATATTGAAAGTAGTTTTCTGATCATAGAT_-_ATGGCACTGAAGAAGGCGTCGAAGCCGGTTTTCCGACTCTTCTCAGCGGAATGCAATGCTCGGCGGACATTCTTTCGGTCGTTGGCGAATTCAGTGGATCAGGGTTTGCAGAGAGATTGCCTTCACCAATCAGGATCTTCGAAATCTCACTGGGGTTCGAATTCGGGGACTTATATGAGGTGGGCTTCGGCGCTCGTCCTCAGTGCTGGTTTGGGTTTACTGCAATTTCCGGAATTCAAAAGTGATTTTAGTAATTCAAACGAAGATCAACGAACGGAAGGACGCACAAACAAATTTATTCTTGGAGATTCATACAGGAGACGACTATTTTTCAACTACGAGAGGAGAATAAGGATGCGAAGTTCTCCTGAAAAGGTATTTGAATACTTTGCATCATTTAGAACTCCTGATGGAGAGTTATTTATGACTCCTGCTGACCTGGTGCGAGCAGTAGTTCCTGTATTTCCACCACCAGATTCTAATATTGTCAGGGATGGATCTTTGAGAGGGGAACGATCTCCTGGAGAACTTCATTGCCCTCAATCAGAGTTATTCATGCGGTTTGATACTAATGGGGATGGCCTTATCTCCTTTCCAGAGTATATTTTTTTCGTAACACTCTTGAGTATTCCTGAATCGGATATTTTAGCTACGATCAAAATGTTTGATCTGGACCGCAATGGGGAGATAAAGAAAGAAGAATTCAAGAAAGTAATGAAATTTTTGCGAGCTAATAACAGGCAAGGGTCTGCTCAACGTGATGGCCTTCGTATAGGACTTAAAGTCGACAAATCTGTGGATAATGGAGGTCTTGTGGAGTATxxxxxxxxxxxxxxxGACGGGAAGGACCGTCTTCAGCTTATGAACTTTGCACAGTTTGTCAAGGATTTGCACAAGGAAATTACACGTCTAGAGTTTTTGCACTATGATCATCAGGGATGTGGTAGCATATCAGCCAGAGATTTTGCGTTGTCCATGGTTGCTGCAGCAGATGTGATATGTGTAAATCGGTACTTGGATCGGGTAGATGATCTCAGCAAGGACCCCAGTCTGAGTAATATACACATTACACGTGAGGAATTTGAGAACTTTGCTGAGTTGCGCAAGAAACTGAAGCCTCTATCATTGGCCATATCCAGTTATGGAAAAGTTTATGGAATCTTGACCAAGTCTGATTTTCGACGAGCAGCATCTCATGTTTGTGACGTTGACGTCACAGGCAACATGGTTGATATCATATTTTATATTTTTGACAAAAATAACGATGGCAACCTTAGCACGGATGAATTTTTGGCAGTATTAGAGCGGCGTGAAAAAGATATTGCATACCCTATAGAGTCTGGGATTATTCACTCGCTGACTTCTTGGTGGAAATGTGCGAAAGATTGTCGTTCGGCATGGAATTATTAG___TGATTTGAAGAACCATTCATTATTATCCCGTGCAAATCAAGGAATTTTACAATGAGGTTTAATGAGACTCCACAATTGCAGCAGGTGCAGGACTTGTGACAGCTACTTGTGTACATTGGAAGGATCAAGCAAATCATATTATCATTGGAAAATCCAGTCATGGTGTCTGGCAATCCAAGAAATTGCCTTCAAGCCCTAGTGACTTAGGCTGGAGGCAGAAAGACCAGCAATGGCTCTTTTCAAATGGTCTTTCGGGAAGTCTTCATTATGGCATCTAACAATTCACATGCTTTCACATTGCCAATTATTTAGAATGCCTAGTGGGTTTAATCGGAGTTCTTGGAGATGACTTGTCGAGTACTGGAAACtGTCTCATGTAAAATATCAATTTTTTGATGTCATCTTTAAACGTAAAAAAACTAATATGTGCTCGCTAGTTAAGAGTGTAACTTAATATCACA

Fln Alignment:

Contig1804___SNEDQRTEGRTNKFILGDSYRRRLFFNYERRIRMRSSPEKVFEYFASFRTPDGELFMTPADLVRAVVPVFPPPDSNIVRDGSLRGERSPGELHCPQSELFMRFDTNGDGLISFPEYIFFVTLLSIPESDILATIKMFDLDRNGEIKKEEFKKVMKFLRANNRQGSAQRDGLRIGLKVDKSVDNGGLVEYxxxxDGKDRLQLMNFAQFVKDLHKEITRLEFLHYDHQGCGSISARDFALSMVAAADVICVNRYLDRVDDLSKDPSLSNIHITREEFENFAELRKKLKPLSLAISSYGKVYGILTKSDFRRAASHVCDVDVTGNMVDIIFYIFDKNNDGNLSTDEFLAVLERREKDIAYPIESGIIHSLTSWWKCAK
Q0DZK7______________AEEEEREEKR--RFLFGDSYRRRVFFNYEKRIRTRSPPEKIFEYFASIRNPEGEVYMLPADLMRAVVPVFPPSESKIVREGSLRGERNPGELHCAPSEFFMLFDTNGDGLISFAEYIFFVTLLSIPESSFNIAFKMFDLDHSGEIDKEEFKKVMALMRSYHRQGAAHRDGLRFGLKVGQSVENGGLVEYxxxxDGNEQLRYDKFSNFLKQLHDEIVRLEFSHYDVKSSKTISVKDFALSMVASADMNHINKLLDRVDDFDDYPDLKDLRITFEEFKAFADLRRKLEPFAMAIFSYGKVNGLLTKQDLKRAATHVCEVDLTDKVVDVIFLVFDANRDGSLSADEFLRALQRRESDIRQPASSGLMGVFTCLLNCTK

SNPs (tot: 46)

Position	A %	C %	G %	T %	Probability
865	12			87	0.992554
866	12			87	0.992554
868	87		12		0.992554
869		87		12	0.992554
897		14	85		0.993533
927	16		83		0.994512
1399		18	81		0.999994
1464	25		75		1.0
1475			28	71	1.0
1498		33		66	1.0
1529		80		20	0.999996
1548		88		11	0.923564
1559		11		88	0.923564
1675		62		37	1.0
1743		12		87	0.992554
1793		75		25	1.0
1847		83		16	0.994512
1877	20		80		0.995493
1880		80		20	0.995493
1930		16		83	0.994512
1977			16	16	0.988813
1979		80	20		0.995493
1983	80		20		0.995493
1984	20			80	0.995493
1986	20		80		0.995493
1988		20		80	0.995493
1989	80	20			0.995493
1990	20	80			0.995493
1991	20	80			0.995493
1993	80		20		0.995493
2000			20	80	0.995493
2122	50	50			1.0
2125	83			16	0.994512
2126	83		16		0.994512
2128		83	16		0.994512
2130			16	83	0.994512
2131	16	83			0.994512
2133	20	80			0.995493
2134			20	80	0.995493
2136		20	80		0.995493
2138	20			80	0.995493
2139		60	40		1.0
2140	20	60	20		0.99255
2141		60		40	1.0
2142	20	80			0.995493
2144	20	20		60	0.99255

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain