SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1797

Length: 1940 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene1797 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	male sterility 2 [Brassica rapa subsp. chinensis]	-	-	47%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	41%
Blast2go	fatty acyl- reductase 2-like	-	-	65%

Source	ECs	Term	Type	e value	Identity
Blast2go	Methionine synthase.	EC:2.1.1.13	-	0.0	65%

Source	KEGGs	Term	Identity
Blast2go	Cysteine and methionine metabolism	00270	65%
Blast2go	Selenocompound metabolism	00450	65%
Blast2go	One carbon pool by folate	00670	65%
Blast2go	Metabolic pathways	01100	65%
Blast2go	Biosynthesis of secondary metabolites	01110	65%

Source	GOs	Term	Type	Identity
Blast2go	response to salt stress	GO:0009651	Biological Process	65%
Blast2go	pollen exine formation	GO:0010584	Biological Process	65%
Blast2go	metabolic process	GO:0008152	Biological Process	65%
Blast2go	response to cadmium ion	GO:0046686	Biological Process	65%
Blast2go	methionine synthase activity	GO:0008705	Molecular Function	65%
Blast2go	fatty-acyl-CoA reductase (alcohol-forming) activity	GO:0080019	Molecular Function	65%
Blast2go	cytosol	GO:0005829	Cellular Component	65%
Blast2go	chloroplast envelope	GO:0009941	Cellular Component	65%
Blast2go	plasma membrane	GO:0005886	Cellular Component	65%
Blast2go	apoplast	GO:0048046	Cellular Component	65%

Source	InterPros	Term	Type	Identity
Sma3	Male sterility	IPR004262	-	-
Sma3	Male sterility, NAD-binding	IPR013120	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-
Sma3	Glycoside hydrolase, family 5, conserved site	IPR018087	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LRT2

Fln msg: No stop codon before M and low homology subject, your sequence is longer than subject: 582 - 510

Fln protein: MSNSLLIKMGKGKKLGTSNQSHRQTVAFNELSLITAKHEMCSSEATLISDDRRSSGVVEATSTSLKNIESTVAGSISVMAPCKGLGIVDFMKDKNFLITGATGFVGKVLIEKILRMQPDIGKLFLIIRANDSASALKRLKDEVMNVELFKCLREIHGDEFEKFMLKRLVPVAGDMTKHNLGIEEEMANVLTEEVDIIVSSAATTTFDERYDVALQTNTTGVDSLLQFGKCCENLQLFIHISTAYVHGQRQGRALEKPLQMKRGIANQKAEFIPPLNFQAEFHLANKVREGLEIEYSNFQRCNKGLVDSQRKITQRMQNLGMERARAYGWQDTYTFTKAMAEMLMVSGRENLPVVIVRPSVIESTYSQPFPGWIEGHRMLDPIITLYGKGQLSCWLADSDTLLDLVPVDMVVNATLAAIAKHAGKPGLKVYQVASSVANPIRVGKVLNLIFEHFKCNPYIDNKGKPVRLMKAITCLHTMEDFCGQDWDTSPSSKHNSVISIDQLKYMAKIYEPYGFYKGRFDNSNLEKLFQELSEEEQEVFGFDVKNIEWKNYIGNIHVRGVRRHVMKGRGSGKSMINISSD*

Protein Length: 583

Fln nts: AAAAGTTTTCCTCTCCCTGGGTCTGGGTCC_-_ATGTCCAACTCTTTGCTGATAAAAATGGGGAAAGGTAAGAAATTGGGAACAAGCAATCAATCCCATCGCCAAACAGTAGCTTTCAACGAGTTATCTCTGATCACTGCAAAACATGAAATGTGTTCTTCGGAAGCTACTTTGATCTCAGATGACAGAAGATCAAGCGGAGTGGTGGAGGCCACAAGTACCAGTCTGAAAAATATTGAGAGTACTGTTGCAGGAAGCATTTCAGTTATGGCTCCATGCAAGGGTCTTGGAATTGTAGATTTTATGAAGGATAAGAACTTTCTCATCACAGGTGCCACTGGTTTTGTTGGAAAAGTGCTGATAGAGAAGATATTAAGGATGCAGCCAGATATAGGAAAGCTGTTTTTGATCATTAGGGCTAACGATTCTGCATCTGCATTGAAGAGATTGAAGGATGAAGTTATGAACGTGGAGTTATTCAAATGTTTACGTGAAATCCATGGTGATGAATTCGAAAAATTCATGTTGAAAAGACTGGTTCCTGTAGCTGGTGACATGACGAAACACAACCTTGGAATAGAAGAGGAAATGGCCAATGTGCTGACGGAAGAAGTAGATATCATTGTCAGCTCTGCAGCCACTACAACATTTGATGAAAGGTATGACGTGGCTCTGCAAACCAACACAACTGGAGTTGATAGCCTTTTGCAGTTCGGCAAATGCTGTGAAAATCTCCAACTTTTCATCCATATATCCACAGCATATGTCCACGGGCAGAGACAAGGTCGAGCACTCGAGAAGCCCCTTCAGATGAAGCGCGGTATAGCAAATCAGAAAGCAGAATTTATTCCACCTCTGAACTTCCAAGCTGAATTTCATTTAGCAAACAAAGTTCGCGAGGGTTTGGAGATTGAATATTCAAATTTCCAGAGATGCAATAAGGGGCTAGTAGATTCTCAAAGGAAAATCACTCAGAGGATGCAAAATTTGGGCATGGAAAGGGCAAGAGCATATGGATGGCAGGATACGTACACATTTACGAAAGCAATGGCCGAGATGCTTATGGTCAGTGGCAGGGAAAATTTACCTGTAGTCATTGTTCGTCCTAGTGTTATTGAAAGCACATACAGTCAGCCTTTTCCTGGATGGATAGAAGGCCATCGAATGTTGGATCCCATCATTACACTCTATGGAAAAGGTCAGCTGTCTTGCTGGTTGGCCGATTCAGATACGCTGCTCGATCTGGTACCTGTAGACATGGTTGTGAATGCAACTTTAGCTGCAATAGCAAAGCATGCCGGAAAACCAGGGCTAAAGGTTTATCAGGTGGCTTCTTCAGTTGCAAATCCTATAAGAGTTGGAAAAGTTCTTAACTTAATATTTGAGCATTTTAAATGTAATCCATACATTGACAACAAAGGGAAACCAGTAAGATTGATGAAGGCAATAACATGCCTGCATACAATGGAGGACTTCTGTGGGCAAGACTGGGACACCAGTCCAAGCAGTAAACACAATTCTGTGATATCAATAGACCAATTGAAGTACATGGCCAAGATCTACGAGCCATATGGATTCTACAAAGGAAGGTTCGATAACTCAAACTTGGAGAAGTTGTTCCAAGAGCTTTCAGAAGAAGAACAAGAGGTTTTTGGATTTGATGTTAAGAATATCGAGTGGAAAAACTATATTGGAAATATTCACGTTAGGGGAGTTCGCCGACATGTCATGAAAGGACGTGGTAGTGGAAAATCCATGATAAACATCTCCTCTGATTAGTTCTCATAAATTATGTAAATTTGCATGGTAACGTCCTCATTTTCTTCATTTTTCAGGTTGAAGAAATCATTATGATTTTTATTCGGCAAACTGAACTCTAAATAGACTTTGGGAAGGATTGTATATATCATTCATAAAGAGCAAAGCTATAAATTGCTTGTTTT

Fln Alignment:

Contig1797___IVDFMKDKNFLITGATGFVGKVLIEKILRMQPDIGKLFLIIRANDSASALKRLKDEVMNVELFKCLREIHGDEFEKFMLKRLVPVAGDMTK-HNLGIEEEMANV-LTEEVDIIVSSAATTTFDERYDVALQTNTTGVDSLLQFGKCCENLQLFIHISTAYVHGQRQGRALEKPLQMKRGIA---NQKAEFIPPLNF--QAEFHLANKVREGLEIEYSNFQRCNKGLVDSQRKITQRMQNLGMERARAYGWQDTYTFTKAMAEMLMVSGRENLPVVIVRPSVIESTYSQPFPGWIEGHRMLDPIITLYGKGQLSCWLADSDTLLDLVPVDMVVNATLAAIAKHAGKPGLKVYQVASSVANPIRVGKVLNLIFEHFKCNPYIDNKGKPVRLMKAITCLHTMEDFCGQDWDTSPS-----SKHNSVISI----------------DQLKYMAKIYEPYGFYKGRFDNSNLEKLFQELSEEEQEVFGFDVKNIEWKNYIGNIHVRGVRRHVMK
B8LRT2______________ILGFLNEKNILVTGSTGFLGKIFVEKILRTQPGVKRIFVLLRSEDFESAKIRFKNEVMGAELFKTLRGQHGDNFENFVFQKVVPVVGDVAGGHNLGIDKESTREHLWNILDAIVNNAASTMFDDRYDISLNVNTRGAENIVEFAKRCRKLQILLHVSTAYVVGRGSGRIKESPLKIGESMTAGNELEDENGPPLSLDIEAEFGLLENTLAKLHMDSSATGTTSTRSVQDKEAI-KHLKELGLERARRFGWPNTYSFTKAMGEMVVENRRENLPVVILRPTIIESTLAEPFPGWMEGTRTMDTFIVGYGKGRISFFLGDPELFLD-IPADMVVNQMMVAMATHAYQNDLFIYHVASSVGNPLRYSLLSDVAYNYFSKNPCVSNDGKIIR-VKEMRFLKSMSSFRLHMFQRYKAPLLVLGVVNEVISIFTARFTARYNQMLRNYNFMMYLAELYEPYVLFQGSFDITNTERLLEKVSVQDLEAFNFDVKCIDWVEYLSNVHIPGVVKYVLK

SNPs (tot: 2)

Position	A %	C %	G %	T %	Probability
1524		20		80	0.995493
1813		12		87	0.992554

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain