SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1468

Length: 1400 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene1468 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	protein pumilio 24 [Arabidopsis thaliana] sp\|Q9LRZ3.1\|PUM24_ARATH RecName: Full=Pumilio homolog 24; Short=APUM-24; Short=AtPUM24 dbj\|BAA95774.1\| unnamed protein product [Arabidopsis thaliana] gb\|AAK73996.1\| AT3g16810/K20I9_3 [Arabidopsis thaliana] gb\|AEE7	-	-	40%
FL-Next	sp=Pumilio homolog 24; Arabidopsis thaliana (Mouse-ear cress).	-	-	42%
Blast2go	protein	-	-	60%

Source	GOs	Term	Type	e value	Identity
Blast2go	nucleolus	GO:0005730	Cellular Component	0.0	60%

Source	InterPros	Term	Type	e value	Identity
Sma3	Pumilio RNA-binding repeat	IPR001313	-	0.0	-
Sma3	Armadillo-like helical	IPR011989	-	0.0	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: sp_plants

Fln subject: Q9LRZ3

Fln msg: your sequence is shorter than subject: 339 - 641

Fln protein: RRDLGCHSFCIIHGGAKERMKIVKGMKGHVVKIAHDEYGCVILMCILSFVDDTSLVSKFIFRELEKDLKELALAKYGRRPLLHLLCPNVPRYFPADVLETLRSTIHSLTTCTQDGPPQSADDEPSESQKPSSETDIPGLKIDEKDEENLEESEGFGKISKKDFSTRRIELLTKSGFAQKLTEMCMQNVGELLRSQYGKDIIFEVANGGAGDIMWQVAADSLTALHRAIADVGALPQLNNEEGSQSDEHIFVQYHSSRTVRKLILESHVPDGVNAPSFASILWDVALKGKCKMWAQGHSEKVVSAFLDCSDLEVKNMASSELQPLIDAGLLKIASAKHV*

Protein Length: 340

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTTTGTATTATACATGGTGGTGCTAAGGAAAGAATGAAGATTGTGAAAGGAATGAAAGGGCATGTAGTAAAGATTGCTCACGATGAATATGGATGTGTGATCCTCATGTGCATTCTCAGTTTTGTTGATGACACCAGTCTTGTTTCTAAGTTTATATTTCGTGAGCTAGAAAAAGATTTGAAAGAATTGGCATTAGCCAAGTATGGGCGGCGTCCACTTTTGCATCTTCTTTGCCCAAATGTACCTCGCTACTTTCCTGCAGATGTTCTGGAGACTCTAAGATCAACGATTCATTCTCTTACTACCTGTACCCAGGATGGGCCTCCCCAGTCTGCAGATGATGAACCTTCTGAAAGTCAAAAGCCATCTTCAGAGACAGATATCCCAGGTCTCAAAATTGATGAAAAAGATGAAGAGAATCTCGAAGAGAGTGAAGGGTTTGGGAAAATATCCAAAAAGGATTTTTCAACTAGAAGAATTGAACTTTTAACTAAGAGTGGATTCGCACAGAAACTTACAGAAATGTGTATGCAAAATGTAGGAGAACTTCTTCGTTCTCAGTATGGGAAGGACATTATTTTCGAGGTAGCAAATGGTGGGGCTGGAGATATTATGTGGCAGGTAGCAGCTGACAGTTTGACCGCTCTTCATAGAGCCATAGCAGATGTGGGTGCGTTGCCTCAACTAAATAATGAAGAAGGCTCTCAAAGTGATGAACATATATTTGTGCAATACCATTCCAGCAGGACAGTACGCAAGTTGATATTGGAGTCTCATGTACCTGATGGTGTCAATGCTCCCTCATTTGCCTCTATTTTGTGGGATGTGGCCTTAAAGGGCAAGTGCAAGATGTGGGCTCAAGGACACAGTGAGAAAGTTGTTTCTGCATTCCTCGATTGTTCAGACTTGGAAGTGAAAAACATGGCAAGTTCTGAGCTACAGCCACTTATTGATGCGGGGTTGTTGAAGATTGCGAGTGCTAAACATGTTTAG___GATGCTTTACAGAAATTTTGATATTGCTTTGACAAGCATGTGCATTGTATAGAAGTAAACTAAGATTCATCATTTATATGTTCTTACATGGTTTGCTCGATGCTCTCGAGAGTAAACTTCATTATAAGAAAATTTTGACATTTGATATTTATAAAATTCATATTTGGAAGAGAACATGCATGTATTCTGTAGGCTGGGATGTTTTAGACTCTAGCGAAATGATAAGGTTCATCATATTCAGTGTTAGAACACACCCTTCCTATATTTAAATGGATGAGATAACGTCTGTAATAAAAGGGCATTCCCCAAGCAAATCTGTGCATAATGGCTTACAGGGTTGATTACGGAAAGCTGATGTATCTAAGATAACATTCTCTTGTATG

Fln Alignment:

Contig1468___IIHGGAKERMKIVKGMKGHVVKIAHDEYGCVILMCILSFVDDTSLVSKFIFRXXXXXXXXXXXXKYGRRPLLHLLCPNVPRYFPADVLETLRSTIHSLTTCTQDGPPQSADDEPSESQKPSSETDIPGLKIDXXXXXXXXXXEGFGKISKKDFSTRRIELLTKSGFAQKLTEMCMQNVGELLRSQYGKDIIFEVANGGAGDIMWQVAADSLTALHRAIADVGALPQLNNEEGSQSDEHIFVQYHSSRTVRKLILESHVPDGVNAPSFASILWDVALKGKCKMWAQGHSEKVVSAFLDCSDLEVKNMASSELQPLIDAGLLKIASAK
Q9LRZ3______________IKHGSAKERKKIIKAMKEHVKKMAFDQFGSMVLACIFSIVDDTKLVTKIIVRELEATLKDLVMDKNGRRPLLQLLHPNSSRYLSHDDLAALDLSVPSL--CSMDKSETSSKTKDTDGNEIGEETKDEQEDTVAEHSDHEENVTAMG--GKKDPLVRRQELLVNSGLAERLIDVCVENAEEFLQSKFGNEVMYEVAIGGSDGILCPSLSEKLCELYEAISSVAAEPK--PQESEKDSEHILENFHSSRTIRRLVL--------NRPGFASILFKKALSGKCRSWAQGHCSKILSAFVETEDVQVREMAKTELQVLVNEGTLKISATK

SNPs (tot: 66)

Position	A %	C %	G %	T %	Probability
310	66			33	0.997452
367	66		33		0.997452
368	66		33		0.997452
414	90		10		0.90298
432		10	90		0.90298
435			90	10	0.90298
496	88			11	0.923564
562	88			11	0.923564
566		11	88		0.923564
568			88	11	0.923564
569			77	11	0.968132
570		77	11		0.968132
572	88		11		0.923564
573	66		22		0.999999
575		11	88		0.923564
577		33	66		1.0
578	22		66	11	0.999999
579	11		88		0.923564
580	66	33			1.0
584	25			62	1.0
585			25	62	1.0
587	62	12		25	1.0
590	12	87			0.992554
591		62	37		1.0
592	87		12		0.992554
593		25		75	1.0
594		75	25		1.0
596		16	83		0.994512
601	83		16		0.994512
776		10	90		0.90298
777	10	90			0.90298
778		90		10	0.90298
779		90		10	0.90298
807		87	12		0.992554
808	12	87			0.992554
809			12	87	0.992554
811	12	75	12		0.975346
812	12	87			0.992554
813	12	75			0.975346
814	88		11		0.923564
815	11		11	77	0.968132
816	88	11			0.923564
817	11	88			0.923564
818	11			88	0.923564
819	11		88		0.923564
820	77		11	11	0.968132
822	88			11	0.923564
824	88		11		0.923564
828	83	16			0.994512
890	20	20			0.99255
899		20		80	0.995493
979		25		75	0.996473
980		75		25	0.996473
1161	14	85			0.993533
1162	85		14		0.993533
1163	85			14	0.993533
1167		14		85	0.993533
1168		14	85		0.993533
1169	14		85		0.993533
1171		85	14		0.993533
1176	14	85			0.993533
1178		85	14		0.993533
1321		25		75	0.996473
1322	25		75		0.996473
1323		75		25	0.996473
1324		75		25	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain