SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1404

Length: 2390 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene1404
ATGGGTATGTCCGTTTTGGGCCTCAAATCAATAATACCCTTGAAGCCAGTCTTCTAAGACCAGGTAGCTGCAAGGTCGATTGCTGTATTGTGGTTGAGACGTCGTGACCTTGGCTGTCACTCATCTGTGTGTTGTTTTCGAGCTGCCCTAGAAATGGATCTGTTATTCTGCTAGACGGAGCAATATGCGAAAACCATGTTGACAGTGGATATAATCGTCTCTTCCTCCGCTGTAAGAGAGGATGAGACGATGCTCAACAAGTGAAAATTCTTGACAGCCCTTGAGTTGGATTGGATGGGGTTTTTGGTCGAGGAAGCTCTATGATTGGAGCCAGCTGGTGCTTTTTGGTATGGGGAATGGAGCAGAGGAACATTTTCTGAGTTGTTGGGTATTAGAAAAGAGTAAGATCCCTTGTCTGTTTCCCATGACATAATGCTGAAGTGTTCTGAGAGCCTTGATCCTCTGGTTAAAGGACAAGTGAGGAGCCAGAAAGTTGAAACAGATGGAGGAAGTGACCACGCCAAGCATTGGGCTGATCAAGATGAGCGATGAAGGCCTTGAAAACCCAGCTGTAGCAGCAAGGCGGCGTCGGAAGGAAATTAGACGCCTAAAGGTTATAGCTAATGCTGTAAATCTGTCGACCGAGCCAATGAGTAAGCGTTCACGACCTATTACTTCTCCCTCTAGTATTGTTGATGGTGATGGAGACATGAGACCTCCCGAACCTGATCTTGATGTTGTAACCAGGTCCTTTGATAGTTTCCCAAATGTCAATGATGATGAGGAAGGAGTAACTGATGATTGTAACCCAGAAACTATGTTTGATCCCTTGCCATCTTCTCTATCTTTGCTGGAGGAGGCAGAGACCTTATCAGTAGAAACTTGCAGCTTGGTTAGCTATAACAGCGAGGAGGAGAGTGTCGCGAGCATGGATATAGACCTGTCACATGAAGATTCTAATATACCATTCGCTAGTGATGATAGTAGCTTCAGTGCAGGAGAACTATGTGGTAAGGAGATTGTAAGTGATAGAAGTACCAACTGTTCAGACTTTGACATTTGTGGATTTGAAACATCTGAGGCTGTTAAAACTTCGGCTGATGTTGTTCCAGAAGGTTCAATTGCAGGCCCTGATCAGGAAATTCCATTTTGTGTAGATCAGCAGTTGGTTTCAGAGATAGATTCTGTAGTTGTAGATGCTCCAAATCGTCTTGACTGTTCAATCCTTGGCACCAGTGGAGGTAGCGTTTCCAGTGGAAAAGTCAATTGCTGTATAGACAGCAACCACATTCCTCCTTGGGCCCATATCTCCATTCCTGGCAGAAGACCGGAGATGGAAGATTCTGTGGCATCTGTGCCTGGCTTTTTCTCTCTTCCTTGTAGAGTTGTTGGGGGTTGCACTGCCAGCGGTTCACAAAAATCTAATGAAAGTTCTCCACTACATTTTTTTGGAGTATATGATGGACATGGAGGTGCCCAGGCTTCCAATTTCTGCAAAGACCGCCTCCATCAGGCACTGGTCGAGGAGTTAGAGGCAGTAATTAGCGGCGAGGGATCCAGCATCGATGGTAGTAATAGCAACTGGAAGAGACTCTGGGAAAGGGCTTTAGTTAGTTGCTTCAAGAAAGTGGATGCTGAAGTTGGACAGGAGCCTAACAGAAGAGATGACTCAGGGGAGGGAAACCACATGGAATGTTGTTCAGAGCCCATTGCCCCTGAAACTGTGGGAACTACTGCTGTTATTGCTATTGTGGGGTCATGTCAGGTTATCATTGCTAATTGTGGAGATTCAAGAGCTGTTTTATGCCGTGGAGGGAAGGCCATTCCACTTTCAGATGATCACAAACCCCAGAGAGCGGACGAGATGGCAAGGATTGAGGCAGCAGGGGGCAAGGTTATTTGTTGGAATGGATATCGTGTTTTAGGAGTGCTGGCAATGTCAAGGGCCATTGGTGATCGCTATTTGAAGCCATCTATTATTCCTGACCCTGATATTACATTCACTGAACGAGTAAAAGAAGATGAATGTCTGATACTGGCCAGTGATGGATTATGGGACGTTATATCAAATGAGGAGGCGTGTGATATTGCTCGAAGGCGCATCGCTCATTGGCATAATAAGAATCCAGTTTCATCTTTAAAATCAATGGATCAGTCAATGGACCCTGCAGCACAGTCAGCTGCCAATCATCTCTGCAAGCTTGCTATTCAGCGTGGCAGCTCTGATAATATAACGGTTTTGGTTGTGGACTTGAAGGCAAAAAGGAGGCGGGCACGGAGAAGCTGAAACCATGAAGCTTAAAGTTACTCACGCTGCATTGATGTTCCTCCGAGGAAAATTGTACCATTAGAGTTGTTGATATCCCAAGGTGATTGCTTTCATTGAAATT

Ace file of the UniGene sp_v2.0_unigene1404 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Protein phosphatase 2C n=2 Tax=Physcomitrella patens RepID=B1B5N7_9BRYO	-	-	40%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	53%
Blast2go	abscisic acid insensitivity 1b	-	-	66%

Source	ECs	Term	Type	e value	Identity
Blast2go	Phosphoprotein phosphatase.	EC:3.1.3.16	-	0.0	66%

Source	GOs	Term	Type	e value	Identity
Blast2go	phosphoprotein phosphatase activity	GO:0004721	Molecular Function	0.0	66%
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	0.0	66%

Source	InterPros	Term	Type	Identity
Sma3	Protein phosphatase 2C, manganese/magnesium aspartate binding site	IPR000222	-	-
Sma3	Protein phosphatase 2C-like	IPR001932	-	-
Sma3		IPR014045	-	-
Sma3	Protein phosphatase 2C	IPR015655	-	-
Sma3	Aldehyde dehydrogenase, conserved site	IPR016160	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A8K0

Fln msg: your sequence is longer than subject: 595 - 533

Fln protein: MEEVTTPSIGLIKMSDEGLENPAVAARRRRKEIRRLKVIANAVNLSTEPMSKRSRPITSPSSIVDGDGDMRPPEPDLDVVTRSFDSFPNVNDDEEGVTDDCNPETMFDPLPSSLSLLEEAETLSVETCSLVSYNSEEESVASMDIDLSHEDSNIPFASDDSSFSAGELCGKEIVSDRSTNCSDFDICGFETSEAVKTSADVVPEGSIAGPDQEIPFCVDQQLVSEIDSVVVDAPNRLDCSILGTSGGSVSSGKVNCCIDSNHIPPWAHISIPGRRPEMEDSVASVPGFFSLPCRVVGGCTASGSQKSNESSPLHFFGVYDGHGGAQASNFCKDRLHQALVEELEAVISGEGSSIDGSNSNWKRLWERALVSCFKKVDAEVGQEPNRRDDSGEGNHMECCSEPIAPETVGTTAVIAIVGSCQVIIANCGDSRAVLCRGGKAIPLSDDHKPQRADEMARIEAAGGKVICWNGYRVLGVLAMSRAIGDRYLKPSIIPDPDITFTERVKEDECLILASDGLWDVISNEEACDIARRRIAHWHNKNPVSSLKSMDQSMDPAAQSAANHLCKLAIQRGSSDNITVLVVDLKAKRRRARRS*

Protein Length: 596

Fln nts: ATGGGTATGTCtGTTTTGGGCCTCAAATCAATAATACCCTTGAAGCCAGTCTTCTAAGACCAGGTAGCTGCAAGGTCGATTGCTGTATTGTGGTTGAGACGTCGTGACCTTGGCTGTCACTCATCTGTGTGTTGTTTTCGAGCTGCCCTAGAAATGGATCTGTTATTCTGCTAGACGGAGCAATATGCGAAAACCATGTTGACAGTGGATATAATCGTCTCTTCCTCCGCTGTAAGAGAGGATGAGACGATGCTCAACAAGTGAAAATTCTTGACAGCCCTTGAGTTGGATTGGATGGGGTTTTTGGTCGAGGAAGCTCTATGATTGGAGCCAGCTGGTGCTTTTTGGTATGGGGAATGGAGCAGAGGAACATTTTCTGAGTTGTTGGGTATTAGAAAAGAGTAAGATCCCTTGTCTGTTTCCCATGACATAATGCTGAAGTGTTCTGAGAGCCTTGATCCTCTGGTTAAAGGACAAGTGAGGAGCCAGAAAGTTGAAACAG_-_ATGGAGGAAGTGACCACGCCAAGCATTGGGCTGATCAAGATGAGCGATGAAGGCCTTGAAAACCCAGCTGTAGCAGCAAGGCGGCGTCGGAAGGAAATTAGACGCCTAAAGGTTATAGCTAATGCTGTAAATCTGTCGACCGAGCCAATGAGTAAGCGTTCACGACCTATTACTTCTCCCTCTAGTATTGTTGATGGTGATGGAGACATGAGACCTCCCGAACCTGATCTTGATGTTGTAACCAGGTCCTTTGATAGTTTCCCAAATGTCAATGATGATGAGGAAGGAGTAACTGATGATTGTAACCCAGAAACTATGTTTGATCCCTTGCCATCTTCTCTATCTTTGCTGGAGGAGGCAGAGACCTTATCAGTAGAAACTTGCAGCTTGGTTAGCTATAACAGCGAGGAGGAGAGTGTCGCGAGCATGGATATAGACCTGTCACATGAAGATTCTAATATACCATTCGCTAGTGATGATAGTAGCTTCAGTGCAGGAGAACTATGTGGTAAGGAGATTGTAAGTGATAGAAGTACCAACTGTTCAGACTTTGACATTTGTGGATTTGAAACATCTGAGGCTGTTAAAACTTCGGCTGATGTTGTTCCAGAAGGTTCAATTGCAGGCCCTGATCAGGAAATTCCATTTTGTGTAGATCAGCAGTTGGTTTCAGAGATAGATTCTGTAGTTGTAGATGCTCCAAATCGTCTTGACTGTTCAATCCTTGGCACCAGTGGAGGTAGCGTTTCCAGTGGAAAAGTCAATTGCTGTATAGACAGCAACCACATTCCTCCTTGGGCCCATATCTCCATTCCTGGCAGAAGACCGGAGATGGAAGATTCTGTGGCATCTGTGCCTGGCTTTTTCTCTCTTCCTTGTAGAGTTGTTGGGGGTTGCACTGCCAGCGGTTCACAAAAATCTAATGAAAGTTCTCCACTACATTTTTTTGGAGTATATGATGGACATGGAGGTGCCCAGGCTTCCAATTTCTGCAAAGACCGCCTCCATCAGGCACTGGTCGAGGAGTTAGAGGCAGTAATTAGCGGCGAGGGATCCAGCATCGATGGTAGTAATAGCAACTGGAAGAGACTCTGGGAAAGGGCTTTAGTTAGTTGCTTCAAGAAAGTGGATGCTGAAGTTGGACAGGAGCCTAACAGAAGAGATGACTCAGGGGAGGGAAACCACATGGAATGTTGTTCAGAGCCCATTGCCCCTGAAACTGTGGGAACTACTGCTGTTATTGCTATTGTGGGGTCATGTCAGGTTATCATTGCTAATTGTGGAGATTCAAGAGCTGTTTTATGCCGTGGAGGGAAGGCCATTCCACTTTCAGATGATCACAAACCCCAGAGAGCGGACGAGATGGCAAGGATTGAGGCAGCAGGGGGCAAGGTTATTTGTTGGAATGGATATCGTGTTTTAGGAGTGCTGGCAATGTCAAGGGCCATTGGTGATCGCTATTTGAAGCCATCTATTATTCCTGACCCTGATATTACATTCACTGAACGAGTAAAAGAAGATGAATGTCTGATACTGGCCAGTGATGGATTATGGGACGTTATATCAAATGAGGAGGCGTGTGATATTGCTCGAAGGCGCATCGCTCATTGGCATAATAAGAATCCAGTTTCATCTTTAAAATCAATGGATCAGTCAATGGACCCTGCAGCACAGTCAGCTGCCAATCATCTCTGCAAGCTTGCTATTCAGCGTGGCAGCTCTGATAATATAACGGTTTTGGTTGTGGACTTGAAGGCAAAAAGGAGGCGGGCACGGAGAAGCTGA___AACCATGAAGCTTAAAGTTACTCACGCTGCATTGATGTTCCTCCGAGGAAAATTGTACCATTAGAGTTGTTGATATCCCAAGGTGATTGCTTTCATTGAAATT

Fln Alignment:

Contig1404___PPWAHISIPGRRPEMEDSVASVPGFFSLP----CRVVGGCTASGSQKSNESSPLHFFGVYDGHGGAQASNFCKDRLHQALVEELEAVISGEGSSIDGSNSNWKRLWERALVSCFKKVDAEVGQE-PNRRDDSGEGNHM-ECCSEPIAPETVGTTAVIAIVGSCQVIIANCGDSRAVLCRGGKAIPLSDDHKPQRADEMARIEAAGGKVICWNGYRVLGVLAMSRAIGDRYLKPSIIPDPDITFTERVKEDECLILASDGLWDVISNEEACDIARRRIAHWHNKNPVSSLKSMDQSMDPAAQSAANHLCKLAIQRGSSDNITVLVVDL
D5A8K0______________PPHSTMSIIGRRREMEDAVSAVPSFFSIPKASSIALLDGFP--GFVQPPLSTALHFFAVYDGHGGSQASVFCKDRFHEALAEELR---NSSPFCIDLND------WSRVMSTCFTKIDMAVGGMCPNGSCGSGDSQKSSDCCQDPIAPENVGSTAVVAIVSPSQLVIANCGDSRAVLSRGGKAIPLSSDHKPEREDELSRIEAAGGRVIFWNGYRVGGFLAMSRAIGDRFLKRYVISEPEVTCTERTHEDECLILASDGLWDVLSNDVVCEVARKCLAGYRPH------RSKGITEDTPVGAAAALLTKLALGRGSGDNISVVVIDL

SNPs (tot: 36)

Position	A %	C %	G %	T %	Probability
1403	75		25		0.996473
1570	85		14		0.993533
1571	85	14			0.993533
1621	85			14	0.993533
1622		85	14		0.993533
1625			14	85	0.993533
1628	14		85		0.993533
1629		14	85		0.993533
1631	85		14		0.993533
1632	85	14			0.993533
1634	14			85	0.993533
1636			14	85	0.993533
1637		85	14		0.993533
1638	85			14	0.993533
1639	85	14			0.993533
1641		14	85		0.993533
1645		85	14		0.993533
1654	83	16			0.994512
1780	20			80	0.995493
1798	25	75			0.996473
2271		40		60	1.0
2272		40	60		1.0
2273	75	25			0.996473
2274			75	25	0.996473
2275		25		75	0.996473
2276		25	75		0.996473
2278		75		25	0.996473
2280	25		75		0.996473
2283		75	25		0.996473
2286			75	25	0.996473
2287			75	25	0.996473
2289		75		25	0.996473
2290	75	25			0.996473
2292			75	25	0.996473
2293	75	25			0.996473
2295	25		75		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain