SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1345

Length: 1755 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene1345 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	WS02752.CR_G02 SS-IL-A-FL-14 Picea sitchensis cDNA clone WS02752_G02 3, mRNA sequence.	-	-	88%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	85%
Blast2go	uncharacterized protein	-	-	48%

Source	InterPros	Term	Type	e value	Identity
Sma3	Xklp2 targeting protein	IPR009675	-	0.0	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9P0B3

Fln msg: Unexpected STOP codon in 5 prime region,

Fln protein: PTINHRWRLLLSVWWTVRYPLADSHLIHYHGRNGQHFRRTGIWRRWRNVRLQVQLLKRRHTLMPITERLPPKELCYSCP*

Protein Length: 81

Fln nts: ACATGGGGACAGTTCACTGATGGCACCTCAGCCATCTTTTCAAGCAGGATTTTTTCCATATTTGGTATTGGACCCCGGCTCATTAATGGATGAAGCTTGCATGCCATGATGATGACTGAGAAAGGGAGGGTTGTATAACTTTCCAATGATTTTGCAGGTTGGGGAAGCCATGGGCGAAGAGGAAGCTTGCTCTGAATGGAGTCCTGATGGTCTTACTGACACTCGTGAGATTAAGGACCATAGCCAACAATCAATCACAGGTGGAGGCTGCTCCTGAGTGTCTGGTGGACGGTTCGGTATCCTTTGGCAGATTCTCACTTGATACATTATCATGGGAGAAATGGTCAGCATTTTCGCAGAACAGGTATCTGGAGGAGGTGGAGAAATGTTCGACTCCAGGTTCAGTTGCTAAAAAGGCGGCATACTTTGATGCCCATTACAGAAAGATTGCCGCCCAAAGAGCTTTGCTACAGCTGTCCCTAA___CAGAAGAAGAGAACAACCGGTACAAGGATATGGATGCCCTAGCTCCTGAAGTGCAAAATGAACAAGTGCCTAATGGTGATCCTGCATTGTATATTGACAATAACGCATGCCATGGATCCAATACAGAGGGTGGGGTTCATGACATGAATAAAGATGAAGAGTCTAAAGAGGAAGTAATCCAGGATACACCTTTACAAATAGATGAAGCTCACAATGAAGAAATACCTCTAGAGGTAGACGTATCTTCTTTAACTGTTGAAACTAATGTACCTTCACAAAATGATGAAGTCCACCATGAAGAAGTACCTCTGGAGGCAGTATCTTCAATTGCTGAGGCCAAATTAGACATTAATAGTGTCCCAGAGGATCTGAATATGAATGGAGCAGAGAAGCTTGCTTTGCCTAAACAAGATAGTACCCTTGCAAAGGTGGAAACCATTCCCAAAGGCCTGGAAGGGGGTGCAATGGGAAAAAAGAAATCTTTGAAGCATGCAAAAGAAGAACCCAGGGAATTCAAACAAAAGAAAAAGCTCCCTTCCACGGAGTCTGTTGCTTCAAATGGACACGTGCAAGCTCAAATGAAGAAGAACAAAACCGAATCTGCAACAAATATCTCAAACAGTCACACAACTAGGCCTCCAAAGCCTCAAGTTTCTTCTTCTATAAAAAATCTTCCCAACGTGTCAACAAAAATGGAAAAGGATGTGATGTCCAAGAGGAAAATAACAGGCATGGACATAAGTCGGAGCCAAAAGCCAGCACCTGTTTCCTTGCACATCTCACTGAGTTTGGGATCCCAACGTCCTAATACCACAGTGCCTCAGCCATTTGCTCTTGCAACAGAAAAACGGGCCTCAATTGTGGAAAATCTTGGAGACAAAGAAATTGTGAAGCGTGCTTTCAAGGCTTTTCGACATAATTTATCAATGTCATCATCTCCAAAAGCTGATATTTCAAGGGGTTCCAGTGATATTGTTGCGGCCCAGGGCCCTGGTGTCCAAGGAAGGAGGTTGGGTGCATTACACCATGCTTCATCTCCCGTTTGTAGACACCTTGAGGAGAATGAAGGAAAATCCAGGAAGTTGGCTGATTCCAAAAAGGGACAAGATAAATGCCATTCAACTGAAAACAGTGCTAATGGGAGAAAATCAATTCCAAAGGTGACTTCAACAACATTTAGCTTGAAAAGTGATGAAAGAGCAGAGAAGCGGAAAGAGTTCTATATGAAGTTAGAAGAAAAATTGAATGCCAAGGAAGACGAAAATAAACATT

Fln Alignment:

Contig1345___RWRLLLSVWWTVRYPLADSHLIHYHGRNGQHFRRTGIWRRWRNVRLQVQLLKRRHTLMPITERLPPKELCYSCP
A9P0B3______________KWNLLLSVWWMVRYLLVDSHLIHYHGRNGQHFPRTDIWRRWRNVRLQVQLLRRRHTLMPITKRLPLKELCYSSP

SNPs (tot: 22)

Position	A %	C %	G %	T %	Probability
510	25		75		0.996473
514		75		25	0.996473
622	75		25		0.996473
714	75		25		1.0
727	12		87		0.992554
901		11		88	0.923564
983	11		88		0.923564
1012	22		77		0.999999
1054		25		75	1.0
1122	11			88	0.923564
1136			62	37	1.0
1226		75	25		0.996473
1278		40	60		1.0
1401		85		14	0.999981
1490		10	90		0.90298
1491		10		90	0.90298
1527	88	11			0.923564
1528		11	88		0.923564
1529		11		88	0.923564
1531	88			11	0.923564
1634	66	33			0.997452
1635		33		66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain