SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1339

Length: 1805 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene1339 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	symplekin [Arabidopsis thaliana] dbj\|BAF00598.1\| hypothetical protein [Arabidopsis thaliana] gb\|AEE30851.1\| symplekin [Arabidopsis thaliana]	-	-	75%
FL-Next	tr=Putative uncharacterized protein; Ricinus communis (Castor bean).	-	-	79%
Blast2go	uncharacterized protein loc100788861	-	-	83%

Source	GOs	Term	Type	e value	Identity
Blast2go	binding	GO:0005488	Molecular Function	0.0	83%
Blast2go	plastid	GO:0009536	Cellular Component	0.0	83%

Source	InterPros	Term	Type	Identity
Sma3	HEAT	IPR000357	-	-
Sma3	DNA methylase, N-6 adenine-specific, conserved site	IPR002052	-	-
Sma3	Glycoside hydrolase, family 5, conserved site	IPR018087	-	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: tr_plants

Fln subject: B9R6Q7

Fln msg: Overlapping hits, possible frame ERROR between 618 and 608, query STOP codon too far from subject stop. Distance to subject end: 59 aas, putative chimeric sequence, your sequence is shorter than subject: 324 - 1390

Fln protein: RRDLGCHSCVYGQAPKAVKQAVHRHIPILVRTIGSSSSELLQVISDPPQGSENLLMQVLHILTEGRTPSRDLIMTVKQLYESKLKDAGILIPMLSSLSKEEVLPIFPRLVDLPLDKFQTALDRILQGSVHTGPALTPAEVLVAIHAIDPEKDGIPLKKVTDACSACFEQRTVFTQQVLAKVLNQLVEQTPLPLLFMRTVIQAxxxxxPTLVDFLFEILSRLVSKQIWKLPKLWVGFLKCAYQTKPHSYRVLLQLPAPQLENALNKHPTLRVPLASYALQPIIRSALPRATLLVLGLAQDSQPVNSISPTPQSMNAGLSGQSTT*

Protein Length: 325

Fln nts: CGTCGTGACCTTGGCTGTCACTCATGTGTCTATGGACAAGCACCAAAGGCTGTAAAGCAGGCAGTCCATCGGCATATCCCTATTCTTGTACGGACCATTGGTTCCTCATCATCAGAATTGCTGCAAGTTATATCTGATCCTCCACAAGGGAGTGAGAATCTTCTCATGCAGGTCTTGCATATTTTGACAGAAGGGAGAACTCCTTCCAGGGACCTGATTATGACTGTCAAACAGTTGTATGAAAGCAAACTAAAGGATGCAGGAATTCTTATTCCAATGTTATCGTCACTGTCAAAAGAAGAGGTATTGCCAATTTTTCCTCGACTTGTTGATCTTCCACTTGACAAGTTCCAAACTGCTCTTGATCGAATACTTCAGGGATCAGTTCACACAGGTCCTGCTTTGACACCTGCTGAAGTTTTGGTTGCAATCCATGCAATTGACCCAGAAAAAGATGGAATACCTTTGAAGAAGGTAACAGATGCTTGTTCTGCATGTTTTGAGCAGCGTACTGTGTTTACCCAGCAAGTTTTGGCAAAAGTGTTGAATCAACTGGTCGAACAGACTCCCCTGCCTTTGCTGTTCATGCGCACCGTCATTCAAGCAxxxxxxxxxxxxxxxCCTACTCTGGTTGACTTTTTATTTGAGATACTGTCTCGTTTAGTGAGCAAGCAGATCTGGAAGTTGCCCAAGTTATGGGTGGGGTTCTTAAAATGTGCATACCAGACAAAGCCACATTCATACCGTGTGCTATTACAGCTACCCGCACCACAGCTTGAAAATGCACTGAACAAGCACCCTACACTCAGGGTACCACTTGCTTCTTATGCTCTCCAACCCATCATACGTTCAGCTCTTCCAAGGGCAACTCTACTGGTTTTAGGGCTTGCTCAGGACAGCCAGCCAGTGAACTCGATCTCCCCAACCCCGCAATCTATGAATGCAGGCCTCTCTGGGCAATCAACGACATAGATATATTCTTTGGAAGTCTGCATCTATTAACTGAAATTCTCTTGCCAGCTCTTCAATGTGATCATTAAGCATTATGGTGAAAGTTTCGGAAGCATTTAATGTGATAATATTAATCCATTAATGGATTACTGCTGCATGCTCTGGATGGGGGATGAGCTGTTATTCTGATAGTTCCACCAAGAACATATGGATCTCTTCAATTGTATAGATTTTAATTTGCAGATTTGCAGAGATAAAAATGCTCCAATGAAACCCCAACTTTCAAATGACATATCAAGCATTGACCTCTTGTTGAAGAGCCCCACTGTATGCTTGTCGGGGAAGGATTTTTGGAGAACGTTTTGGAAGCACGTGGATTGTTGAATGGAGGCGGGACTGGGTTATGTCATGGGAGATCGTCAGCAATGTCCAGCCTGTTTTTATCTTCAAAAGAATTCTAGCCGTGATATTTCACCATCATGGGAGGAAAACGCTTGTTATTGTCGGATGCTTGTTAGGGAGATTGTACAGCTTGCAGGGTTTAAACCAGGTTCTGCTTCTTGTTCATTTATTGAACAAATACGCAGATATATGTACAATATCAGTTATGTCACATTGAAGATAAGATTGGTTTATTTTCATTTCCACTGGTTTTAATTCAATTGTGTTATATTAAATTACTGTATGTACAAAAATACCCAGGTCTTGGGGTGCTTCCACAGCCTTTTAATAATCCCACTAACATAGGAAGTTCTTCGTTTGACGATCAGAAAATGTCACTGAATTGGTCAATGTATTTCTAATTTACACGAAACTGTCCATAATCTTCTTGTTACGGTGGAATGGCTGGTTCACG

Fln Alignment:

Contig1339___VYGQAPKAVKQAVHRHIPILVRTIGSSSSELLQVISDPPQGSENLLMQVLHILTEGRTPSRDLIMTVKQLYESKLKDAGILIPMLSSLSKEEVLPIFPRLVDLPLDKFQTALDRILQGSVHTGPALTPAEVLVAIHAIDPEKDGIPLKKVTDACSACFEQRTVFTQQVLAKVLNQLVEQTPLPLLFMRTVIQAxxxxPTLVDFLFEILSRLVSKQIWKLPKLWVGFLKCAYQTKPHSYRVLLQLPAPQLENALNKHPTLRVPLASYALQPIIRSALPRATLLVLGLAQDSQ
B9R6Q7______________IYGRAPKSVKQAVHRHIPILIRALGSSCSELLRVISDPPEGCENLLMLVLQKLTQETTPSADLIATVKHLYETKLKDATILIPILSSLSKNEVLPIFPRLVGLPIEKFQMALAHILQGSAHTGPALTPAEVLVAIHDISPEKDGLALKKITDACSACFEQRTVFTQQVLAKALNQMVDQTPLPLLFMRTVIQAxxxxPTLVDFVMEILSKLVTRQVWKMPKLWVGFLKCVSQARPHSFRVLLQLPPPLLESAMSKHSNLRGPLAAFANQPSIRTSLPRSTLAVLGLLNDSQ

SNPs (tot: 39)

Position	A %	C %	G %	T %	Probability
976	62	37			1.0
977			62	37	1.0
1066		12		87	0.992554
1125		50		50	1.0
1185	87		12		0.992554
1187	12		87		0.992554
1212	40		60		1.0
1245		37		62	1.0
1277		75		25	1.0
1278			28	71	1.0
1279	28			71	1.0
1284	28		71		1.0
1285		28	71		1.0
1288	71		28		1.0
1289	71	28			1.0
1292	28	71			1.0
1293	71		28		1.0
1299	28	71			1.0
1300		28		71	1.0
1369		33	66		0.997452
1727		12	87		0.992554
1728	12		87		0.992554
1729			87	12	0.992554
1730	87			12	0.992554
1734			85	14	0.993533
1735		85	14		0.993533
1736	14	85			0.993533
1744	14			85	0.993533
1748	14			85	0.993533
1749	14		85		0.993533
1750		85	14		0.993533
1751			14	85	0.993533
1753		14		85	0.993533
1756	85		14		0.993533
1757	14		85		0.993533
1759		80	20		0.995493
1774	75	25			0.996473
1775		25		75	0.996473
1777			75	25	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain