SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1333

Length: 1095 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene1333 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	haloacid dehalogenase-like hydrolase domain-containing protein [Arabidopsis thaliana] gb\|AAO42848.1\| At1g79790 [Arabidopsis thaliana] dbj\|BAE99879.1\| hypothetical protein [Arabidopsis thaliana] gb\|AEE36298.1\| haloacid dehalogenase-like hydrolase domain-co	-	-	59%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	84%
Blast2go	haloacid dehalogenase-like hydrolase domain-containing protein	-	-	74%

Source	GOs	Term	Type	e value	Identity
Blast2go	hydrolase activity	GO:0016787	Molecular Function	0.0	74%
Blast2go	chloroplast	GO:0009507	Cellular Component	0.0	74%

Source	InterPros	Term	Type	Identity
Sma3	Haloacid dehalogenase-like hydrolase	IPR005834	-	-
Sma3	HAD-superfamily hydrolase, subfamily IA, variant 3	IPR006402	-	-
Sma3	HAD-superfamily hydrolase, subfamily IA, variant 1	IPR006439	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AC02

Fln msg: atg_distance in limit (1-15): atg_distance = 1,

Fln protein: MGIGIDVATHHGLSHGFLISQIPLEHQRRDLGCHSISRTSPQRSIMSFAHLSSLPDVNISKGRKLPIFLFDVMGTIVRDPFYEDVPAFFGLSMKELLEVKHPTAWIEFEKGIITEEELAIKFFSDGRGFDFGGLKQCMSNGYAYLYGIEDILRRLKLNGYEMHAFTNYPCWYSMIENKLKLSTYLSWTFSSCETGKRKPEIEAYSEVSQHLGVPPSSCLFIDDRLANVEVASKLGMTGILFKNAYKLEQDLISLGIEAGSLQ*

Protein Length: 264

Fln nts: _-_ATGGGGATTGGCATTGACGTTGCGACACATCATGGCCTTAGCCATGGCTTTCTCATCTCACAAATTCCTCTCGAACACCAACGTCGTGACCTTGGCTGTCACTCAATTTCTCGGACTTCGCCACAGAGATCAATTATGAGCTTTGCCCATTTGTCATCTCTGCCTGATGTCAACATCTCAAAGGGGAGAAAACTGCCCATTTTCCTGTTTGATGTGATGGGCACCATTGTTCGGGATCCATTCTATGAAGATGTCCCTGCTTTCTTCGGATTATCAATGAAAGAGCTTCTGGAGGTCAAACATCCAACAGCTTGGATTGAATTTGAGAAGGGTATCATTACTGAGGAGGAATTGGCTATCAAATTCTTTAGTGATGGCAGAGGTTTTGACTTTGGTGGTTTGAAGCAATGTATGAGCAATGGATATGCTTATCTTTATGGCATTGAGGATATACTTCGACGACTAAAATTGAATGGTTATGAAATGCATGCTTTTACAAACTATCCATGTTGGTACTCGATGATCGAGAATAAACTAAAACTTTCAACATACTTATCTTGGACATTTTCTTCATGTGAAACCGGAAAGCGAAAACCTGAAATTGAAGCATACTCGGAAGTTTCCCAGCATTTGGGAGTGCCTCCATCTAGCTGTCTCTTCATTGATGACAGACTTGCAAATGTTGAGGTTGCATCAAAGTTGGGTATGACTGGAATTCTCTTCAAGAATGCTTATAAGCTTGAGCAAGATTTAATTTCTCTAGGCATTGAAGCAGGCAGCCTTCAGTAA___AAAATTCAAGTCATCCAATAATTGATACAAATTTAGCTTTGCAGCAAGGAATCACATGCATTTCCAAATTCTGTAAGCATTTGATATGTATGACATTCCGCACATACTGCAAAACTGTTAGCGGTAATAATTGGTTTATGTGCTTCTTGTATGCTTATTGTTTACATTGTAGAATAAGTTTCAACCCCAAAATGCTAAAATAGTATTTACTGAACATTGCAAAATTTTGTTGCAGTGGAAATTTTCCAAAGGTACAGTCGAATGATTGCTTGAGCTTTATTATAAATTTGTTTCATCTTCAAGTAC

Fln Alignment:

Contig1333___MGIGIDVATHHGLSHGFLISQIPLEHQRRDLGCHSISRTSPQRSIMSFAHLSSLPDVNISKGRKLPIFLFDVMGTIVRDPFYEDVPAFFGLSMKELLEVKHPTAWIEFEKGIITEEELAIKXXXXXXXXXXXGLKQCMSNGYAYLYGIEDILRRLKLNGYEMHAFTNYPCWYSMIENKLKLSTYLSWTFSSCETGKRKPEIEAYSEVSQHLGVPPSSCLFIDDRLANVEVASKLGMTGILFKNAYKLEQDLISLGIEAGS
D5AC02______________MGSVIASSTHKFLSN----PKIPLGI--------AISRTSPQRSIMSFTHSSSLPDVNISKGRKLPILLFDVMDTIVRDPFYEDVPFFFGLSMKELLGVKHPTAWIEFEKGIITEEELAIKFFSDGRAFDFDGLKQCMSNGYAYLDGIEDILRRLKLNGYEMHAFTNYPCWYLMIEEKLKLSTYLSWTFSSCETGKRKPEIEAYLEVSKHLGVPPSSCLFIDDRLANVEVASKLGMAGILFKNAYKLEQDLISRGIEAGS

SNPs (tot: 21)

Position	A %	C %	G %	T %	Probability
722	85		14		0.993533
950			88	11	0.923564
958		11		88	0.923564
960	88	11			0.923564
961			11	88	0.923564
964		12		87	0.992554
985		14	85		0.993533
986	85	14			0.993533
987		14	85		0.993533
988	85	14			0.993533
990	85			14	0.993533
996		33		66	1.0
999		66		33	1.0
1000	66	16			0.988813
1001		16	83		0.994512
1004	83		16		0.994512
1005	83			16	0.994512
1009		80	20		0.995493
1010	80		20		0.995493
1014	20	80			0.995493
1015			80	20	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain