SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1321

Length: 2433 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene1321
CCTGCATAGGCATGAAAGACTCGAAGGAATTTGCGGTGGAACTCTTTGATGCATTAGCTCGGCGAAAAGGGCTGAAAATCAATTGCTTGTCCAGGGACGAGCTCTACCAATTCTGGCAGGAGATGGCCGACCAGAACTTTGATTCTAGGCTACAGATCTTCTTTGACATGTGTGATAAGAATGGAGATGGCCGAATATCAGAAGATGAGGTTAAGGAGGTTATTGTGCTGAGTGCTTCTGCAAACAAACTGGCAAAGCTGAAGGAGAATGCTGCAGAATATGCTGGTCTCATCATGGAGGAGCTTGATCCAGATCATTTAGGATACATTGAGATATGGCAGCTTGAAGCTCTGCTACAAGGCATGGTGAACACTTATGGGAACGAAGGATGTGTAAAGCACAGCAAAACTTTGAGTAAGACTATGATTCCCAAGCGTTGGAGAAGTCCCATTTACAGATTCCTTGAGGTGGCAAAGGATTTTGTCCACGAGAACTGGAAGCGCATCTGGATCATCTTACTGTGGATAGCCTTGAATGCTTGTCTGTTCACATGGAAATTTATGCAATACAAGCACAAAGCTGCATTCCAGGTGATGGGTTACTGTGTTTGTGTAGCCAAGGGCGCTGCTGAGACGTTGAAGCTTAACATGGCTCTCATTCTCTTCCCTGTATGTCGCAACACAATCACCTGGCTCAGAAAGACCAGGGCTAGTTCAATTGTCCCGTTTGATGACAATATCAACTTTCATAAGGTGATGGCGGTGGGAATTACAACTGGAGTATTCATTCATGGGGTGGTGCATCTCTCCTGCGACTTCCCCAGAATTGTTTCATGTCCTGACCTCAAGTTTATGGATACAATAGGACGTGGATTCCACTATAGAAAACCAACTTATCAAGATCTGATAACCAGCACAGTGGGCTTAACTGGAATTATTATCGTGTTCCTTATGGCCTTCTCCTTCACGCTGGCCACTCATAGTTTCAGGAGAAACGTAGTCAAACTGCCATGGCCTCTGCATAAGCTAGCTGGCTTCAATGCTTTCTGGTATGCTCATCATTTGTTTATTGCGGTCTACACCCTGCTTATCATCCACTCCATCTTCATATATCTCACACAAGAATGGTACCAGAAAACGGCTTGGATGTACATGGCCATACCCATACTTCTGTACATCGGCGAGCGATTGCTCAGATTTTTCCGTGAAAATACCAACAAAGTTAATCTCGTGAAGGCTGCCATATATCCTGGTAATGTTCTTGCTTTGCATATGACCAAGCCTATGGGATTCAAGTACAAAAGTGGGATGTATTTGTTTATAAAGTGTCCAGATGTCTCTCCATTTGAATGGCATCCTTTTTCTATTACCTCAGCTCCCGGAGACGATTACTTGAGTGTCCACATCCGAACCATGGGTGATTGGACAACAGAACTTAAGAGGATATTTTCTAGGGTTTGTGAACCCCCTACTGCCAATAAAAGTGGTCTACTGAGGGCTGACACTACACTGGTAGCTGACAACGGAGTCCCAGTATGCAGATTTCCCAAGCTTTACATCGATGGACCTTATGGAGCTCCAGCCCAAAATTACAACAAATATGATATTCTGTTACTCGTGGGTTTGGGAATTGGGGCAACGCCTTTCATCAGCATTCTTAAAGATCTTCTGAACAACATCAAGTCTAACGAAGAACAGGCTGAAGCTGGAAATGATGTGAGCAAATTCAAACCCAGTCCAACACGAAGTCCTGAAGGAAGTAATTACAAAGGACGAGTACCGGCCCGGGCATACTTCTACTGGGTTACTAGAGAACAAGGATCCTTTGAATGGTTCAAAGGTGTCATGAACGAAGTGGCAGAGAGCGATCACAACAGAGTGATCGAAATGCACAATTATTTAACGAGCGTGTACGAAGAAGGCGACGCGCGTTCGGCTCTCATAGCCATGGTTCAATCCCTGCATCATGCCAAAAATGGATTCGACATTGTTTCAGGCAGCAGAATTCGAACACACTTTGCGAGGCCAAATTGGAACAGAGTCTTCACCCAACTGTCCACAGCCCACAAGGGTGCTCGAATAGGGGTATTTTACTGTGGGCCGCCTACTCTTACAAAGCAATTACGTTCACTTTCGCAGCAATTTACCCATAACTCAAGCACTCGTTTTGAATTTCACAAGGAGAATTTCTAGCAGAATGGAATTTTTTCCAGATTGTCATTCGTGGGGACACCATCAGTGTGTCTCCGGGTTACAACCACATCCATCCATTTTCCAGTGCTTCGAGAAGAGGATGGTATATGACCACTTGAGTTTGAAATATATTTTCACATACATGTATTCATGTAATAATCCACGATGTTACATCCACGATGGAGGCTGTTATTTTGTTGCATATTTCTATGTCAATGCATGGGAGGGCCCTCTCGGTTTT

Ace file of the UniGene sp_v2.0_unigene1321 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	putative respiratory burst oxidase [Oryza sativa Japonica Group]	-	-	65%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	64%
Blast2go	respiratory burst oxidase homolog protein h-like	-	-	78%

Source	ECs	Term	Type	e value	Identity
Blast2go	Peroxidase.	EC:1.11.1.7	-	0.0	78%

Source	KEGGs	Term	Type	Identity
Blast2go	Phenylalanine metabolism	00360		78%
Blast2go	Methane metabolism	00680		78%
Blast2go	Phenylpropanoid biosynthesis	00940		78%
Blast2go	Biosynthesis of phenylpropanoids	01061		78%
Blast2go	Metabolic pathways	01100		78%
Blast2go	Biosynthesis of secondary metabolites	01110		78%
Blast2go	Oxidoreductases, Acting on NADH or NADPH, With a oxygen as acceptor.	EC:1.6.3.-	-	78%

Source	GOs	Term	Type	Identity
Blast2go	electron transport chain	GO:0022900	Biological Process	78%
Blast2go	flavin adenine dinucleotide binding	GO:0050660	Molecular Function	78%
Blast2go	iron ion binding	GO:0005506	Molecular Function	78%
Blast2go	peroxidase activity	GO:0004601	Molecular Function	78%
Blast2go	electron carrier activity	GO:0009055	Molecular Function	78%
Blast2go	calcium ion binding	GO:0005509	Molecular Function	78%
Blast2go	oxidoreductase activity, acting on NADH or NADPH, oxygen as acceptor	GO:0050664	Molecular Function	78%
Blast2go	integral to membrane	GO:0016021	Cellular Component	78%

Source	InterPros	Term	Type	Identity
Sma3	Cytochrome b245, heavy chain	IPR000778	-	-
Sma3	Calcium-binding EF-hand	IPR002048	-	-
Sma3	EF-hand-like domain	IPR011992	-	-
Sma3	FAD-binding 8	IPR013112	-	-
Sma3	Ferric reductase, NAD binding	IPR013121	-	-
Sma3	Flavoprotein transmembrane component	IPR013130	-	-
Sma3	NADPH oxidase Respiratory burst	IPR013623	-	-
Sma3	Ferredoxin reductase-type FAD-binding domain	IPR017927	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	EF-hand	IPR018248	-	-
Sma3	EF-HAND 2	IPR018249	-	-
Sma3	Chaperonin Cpn60, conserved site	IPR018370	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ABF5

Fln msg: your sequence is longer than subject: 727 - 358

Fln protein: MKDSKEFAVELFDALARRKGLKINCLSRDELYQFWQEMADQNFDSRLQIFFDMCDKNGDGRISEDEVKEVIVLSASANKLAKLKENAAEYAGLIMEELDPDHLGYIEIWQLEALLQGMVNTYGNEGCVKHSKTLSKTMIPKRWRSPIYRFLEVAKDFVHENWKRIWIILLWIALNACLFTWKFMQYKHKAAFQVMGYCVCVAKGAAETLKLNMALILFPVCRNTITWLRKTRASSIVPFDDNINFHKVMAVGITTGVFIHGVVHLSCDFPRIVSCPDLKFMDTIGRGFHYRKPTYQDLITSTVGLTGIIIVFLMAFSFTLATHSFRRNVVKLPWPLHKLAGFNAFWYAHHLFIAVYTLLIIHSIFIYLTQEWYQKTAWMYMAIPILLYIGERLLRFFRENTNKVNLVKAAIYPGNVLALHMTKPMGFKYKSGMYLFIKCPDVSPFEWHPFSITSAPGDDYLSVHIRTMGDWTTELKRIFSRVCEPPTANKSGLLRADTTLVADNGVPVCRFPKLYIDGPYGAPAQNYNKYDILLLVGLGIGATPFISILKDLLNNIKSNEEQAEAGNDVSKFKPSPTRSPEGSNYKGRVPARAYFYWVTREQGSFEWFKGVMNEVAESDHNRVIEMHNYLTSVYEEGDARSALIAMVQSLHHAKNGFDIVSGSRIRTHFARPNWNRVFTQLSTAHKGARIGVFYCGPPTLTKQLRSLSQQFTHNSSTRFEFHKENF*

Protein Length: 728

Fln nts: CCTGCATAGGC_-_ATGAAAGACTCGAAGGAATTTGCGGTGGAACTCTTTGATGCATTAGCTCGGCGAAAAGGGCTGAAAATCAATTGCTTGTCCAGGGACGAGCTCTACCAATTCTGGCAGGAGATGGCCGACCAGAACTTTGATTCTAGGCTACAGATCTTCTTTGACATGTGTGATAAGAATGGAGATGGCCGAATATCAGAAGATGAGGTTAAGGAGGTTATTGTGCTGAGTGCTTCTGCAAACAAACTGGCAAAGCTGAAGGAGAATGCTGCAGAATATGCTGGTCTCATCATGGAGGAGCTTGATCCAGATCATTTAGGATACATTGAGATATGGCAGCTTGAAGCTCTGCTACAAGGCATGGTGAACACTTATGGGAACGAAGGATGTGTAAAGCACAGCAAAACTTTGAGTAAGACTATGATTCCCAAGCGTTGGAGAAGTCCCATTTACAGATTCCTTGAGGTGGCAAAGGATTTTGTCCACGAGAACTGGAAGCGCATCTGGATCATCTTACTGTGGATAGCCTTGAATGCTTGTCTGTTCACATGGAAATTTATGCAATACAAGCACAAAGCTGCATTCCAGGTGATGGGTTACTGTGTTTGTGTAGCCAAGGGCGCTGCTGAGACGTTGAAGCTTAACATGGCTCTCATTCTCTTCCCTGTATGTCGCAACACAATCACCTGGCTCAGAAAGACCAGGGCTAGTTCAATTGTCCCGTTTGATGACAATATCAACTTTCATAAGGTGATGGCGGTGGGAATTACAACTGGAGTATTCATTCATGGGGTGGTGCATCTCTCCTGCGACTTCCCCAGAATTGTTTCATGTCCTGACCTCAAGTTTATGGATACAATAGGACGTGGATTCCACTATAGAAAACCAACTTATCAAGATCTGATAACCAGCACAGTGGGCTTAACTGGAATTATTATCGTGTTCCTTATGGCCTTCTCCTTCACGCTGGCCACTCATAGTTTCAGGAGAAACGTAGTCAAACTGCCATGGCCTCTGCATAAGCTAGCTGGCTTCAATGCTTTCTGGTATGCTCATCATTTGTTTATTGCGGTCTACACCCTGCTTATCATCCACTCCATCTTCATATATCTCACACAAGAATGGTACCAGAAAACGGCTTGGATGTACATGGCCATACCCATACTTCTGTACATCGGCGAGCGATTGCTCAGATTTTTCCGTGAAAATACCAACAAAGTTAATCTCGTGAAGGCTGCCATATATCCTGGTAATGTTCTTGCTTTGCATATGACCAAGCCTATGGGATTCAAGTACAAAAGTGGGATGTATTTGTTTATAAAGTGTCCAGATGTCTCTCCATTTGAATGGCATCCTTTTTCTATTACCTCAGCTCCCGGAGACGATTACTTGAGTGTCCACATCCGAACCATGGGTGATTGGACAACAGAACTTAAGAGGATATTTTCTAGGGTTTGTGAACCCCCTACTGCCAATAAAAGTGGTCTACTGAGGGCTGACACTACACTGGTAGCTGACAACGGAGTCCCAGTATGCAGATTTCCCAAGCTTTACATCGATGGACCTTATGGAGCTCCAGCCCAAAATTACAACAAATATGATATTCTGTTACTCGTGGGTTTGGGAATTGGGGCAACGCCTTTCATCAGCATTCTTAAAGATCTTCTGAACAACATCAAGTCTAACGAAGAACAGGCTGAAGCTGGAAATGATGTGAGCAAATTCAAACCCAGTCCAACACGAAGTCCTGAAGGAAGTAATTACAAAGGACGAGTACCGGCCCGGGCATACTTCTACTGGGTTACTAGAGAACAAGGATCCTTTGAATGGTTCAAAGGTGTCATGAACGAAGTGGCAGAGAGCGATCACAACAGAGTGATCGAAATGCACAATTATTTAACGAGCGTGTACGAAGAAGGCGACGCGCGTTCGGCTCTCATAGCCATGGTTCAATCCCTGCATCATGCCAAAAATGGATTCGACATTGTTTCAGGCAGCAGAATTCGAACACACTTTGCGAGGCCAAATTGGAACAGAGTCTTCACCCAACTGTCCACAGCCCACAAGGGTGCTCGAATAGGGGTATTTTACTGTGGGCCGCCTACTCTTACAAAGCAATTACGTTCACTTTCGCAGCAATTTACCCATAACTCAAGCACTCGTTTTGAATTTCACAAGGAGAATTTCTAG___CAGAATGGAATTTTTTCCAGATTGTCATTCGTGGGGACACCATCAGTGTGTCTCCGGGTTACAACCACATCCATCCATTTTCCAGTGCTTCGAGAAGAGGATGGTATATGACCACTTGAGTTTGAAATATATTTTCACATACATGTATTCATGTAATAATCCACGATGTTACATCCACGATGGAGGCTGTTATTTTGTTGCATATTTCTATGTCAATGCATGGGAGGGCCCTCTCGGTTTT

Fln Alignment:

Contig1321___VYTLLIIHSIFIYLTQEWYQKTAWMYMAIPILLYIGERLLRFFRENTNKVNLVKAAIYPGNVLALHMTKPMGFKYKSGMYLFIKCPDVSPFEWHPFSITSAPGDDYLSVHIRTMGDWTTELKRIFSRVCEPPTANKSGLLRADTTLVADNGVPVCRFPKLYIDGPYGAPAQNYNKYDILLLVGLGIGATPFISILKDLLNNIKSNEEQAEAGNDVSKFKPSPTRSPEGSNYKGRVPARAYFYWVTREQGSFEWFKGVMNEVAESDHNRVIEMHNYLTSVYEEGDARSALIAMVQSLHHAKNGFDIVSGSRIRTHFARPNWNRVFTQLSTAHKGARIGVFYCGPPTLTKQLRSLSQQFTHNSSTRFEFHKENF
D5ABF5______________VYTLLIVHGVFLYLTHKWYKKTTWMYLAVPVLLYGGERILRAFRSGSKPVQILKVAIYPGNVLTLHMSKPQGFRYKSGQYMFVNCPAVSSFEWHPFSITSAPGDNYLSVHIRTLGDWTRELKNSFAEVCEPPMGGKSGLLRADYMQGKNNP----RFPRLLIDGPYGAPAQDYKKYDVLLLVGLGIGATPFISILKDIVNHMKVSEEERNIEN-VGKQKKKTFNT-----------VRAFFYWVTREEGSFDWFKGVMNEVAEIDQKGIIELHNYCTSVYEEGDARSALIAMLQALNHAKHGVDIVSGTRVCTHFAKPNWRNVFKSITLKHKDSRVGVFYCGPAALTDDLRQLSLDFTRKTSTRFDFHKENF

SNPs (tot: 21)

Position	A %	C %	G %	T %	Probability
124		75	25		0.996473
152	50			50	1.0
427	12		87		0.992554
502	90			10	0.90298
669		12		87	0.992554
670	87	12			0.992554
779	87		12		0.992554
911	16		83		0.994512
947	83		16		0.994512
1043	28		71		1.0
1095	28		71		1.0
1096	71			28	1.0
1098	71			28	1.0
1186			83	16	0.994512
1594	25	75			0.996473
1596			75	25	0.996473
1598	75			25	0.996473
1811		50	50		1.0
1829	66		33		0.997452
2182		80		20	0.995493
2184		20		20	0.99255

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain