SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1288

Length: 1392 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene1288 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Serine racemase, putative n=1 Tax=Ricinus communis RepID=B9RC97_RICCO	-	-	68%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	95%
Blast2go	serine racemase	-	-	84%

Source	ECs	Term	Type	e value	Identity
Blast2go	Threonine ammonia-lyase.	EC:4.3.1.19	-	0.0	84%

Source	KEGGs	Term	Identity
Blast2go	Glycine, serine and threonine metabolism	00260	84%
Blast2go	Valine, leucine and isoleucine biosynthesis	00290	84%
Blast2go	Metabolic pathways	01100	84%
Blast2go	Biosynthesis of secondary metabolites	01110	84%

Source	GOs	Term	Type	Identity
Blast2go	serine family amino acid metabolic process	GO:0009069	Biological Process	84%
Blast2go	pyridoxal phosphate binding	GO:0030170	Molecular Function	84%
Blast2go	serine racemase activity	GO:0030378	Molecular Function	84%
Blast2go	L-threonine ammonia-lyase activity	GO:0004794	Molecular Function	84%

Source	InterPros	Term	Type	e value	Identity
Sma3	Pyridoxal phosphate-dependent enzyme, beta subunit	IPR001926	-	0.0	-
Sma3	Serine/threonine dehydratase, pyridoxal-phosphate-binding site	IPR000634	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NZE1

Fln msg:

Fln protein: MRRTLAGTHCSLVSLRLIRSFILVSVKMEAAEFATELESSKQQTNYAADISSIQEARDRIGPYIDKTPVLSSSSLNIIAGRTLFFKCEIFQKGGAFKFRGACNAVFSLPVDLADKGVVTHSSGNHAAALALAAKLRGIPAYIVIPQNAPICKVDNVKRYGGQVIWCEPTMQSRESVSAKVQEETGAVLIHPFNDGRIISGQGTISLELLEKVPQLDAIVVPISGGGLISGVALAAKALKPNILIFAAEPKGADDAARSKAAGHIVTLTETKTVADGLRAYLGELTWPIVRDLVDEIIVVEEGEIIEAMKMCYEILKLAVEPSGAIGLAAILSHQFQDNLRWSHCQNIGIVLSGGNVDLSVLWESFHKAH*

Protein Length: 371

Fln nts: _-_ATGCGACGGACCTTGGCTGGCACTCACTGTTCTCTGGTTTCTTTGAGATTAATCCGATCATTCATACTTGTTTCAGTCAAAATGGAAGCAGCAGAGTTTGCCACAGAATTGGAAAGTTCCAAACAACAGACAAATTATGCTGCAGATATTTCATCAATACAAGAAGCTAGAGATCGTATTGGACCATATATAGATAAAACACCAGTTCTTTCTTCATCATCTTTGAACATTATTGCTGGAAGGACTCTTTTCTTCAAGTGTGAAATCTTCCAAAAGGGTGGAGCCTTTAAATTTAGAGGTGCTTGCAATGCTGTGTTTTCTCTTCCAGTGGATTTGGCAGACAAGGGGGTTGTGACTCACAGCAGTGGTAATCATGCAGCAGCATTGGCTTTGGCTGCAAAACTGCGTGGCATTCCAGCATATATTGTAATCCCCCAAAATGCTCCAATTTGTAAAGTAGACAATGTTAAACGATATGGTGGACAGGTCATTTGGTGTGAACCGACAATGCAATCAAGAGAGAGTGTATCTGCTAAAGTACAGGAAGAAACTGGTGCAGTATTGATTCACCCATTTAATGATGGCCGTATCATAAGTGGGCAAGGTACAATTTCTCTGGAACTCTTAGAGAAAGTACCGCAGCTTGATGCGATTGTTGTTCCAATAAGTGGGGGTGGATTGATCTCAGGTGTAGCACTTGCAGCAAAAGCACTGAAGCCTAATATTCTCATATTTGCGGCTGAACCAAAAGGTGCAGATGATGCTGCACGATCAAAAGCAGCAGGACATATTGTGACTCTCACTGAAACTAAGACTGTTGCAGATGGACTAAGGGCTTATCTTGGAGAACTGACATGGCCCATTGTACGAGATTTGGTAGATGAAATTATTGTTGTTGAGGAGGGAGAGATTATAGAAGCCATGAAGATGTGCTATGAAATTCTAAAGCTGGCGGTAGAGCCCAGTGGAGCCATTGGATTAGCTGCTATACTCTCTCATCAGTTCCAAGATAATTTGAGATGGTCACATTGTCAAAATATAGGAATTGTGCTTTCTGGGGGTAATGTTGATCTTTCCGTCCTGTGGGAATCATTTCACAAAGCACATTAA___AGTTCATTATCTCTAGTTTGTTTTGCAGTTTAAAAATGCAGAACAAGAGAATATTATACCTTGCACACGTTTGTGCATGAATCACAACCTATTTATTTGTATTTTAAGCCAATATTACAGATAGAAGACAGGATACAAATGTCCAGGAAGGATTCTCACAGATGCTTTCATGAATTAGTCTCCTTAACTAGATGAAGTGTTAGATCCAGCATACAATAACACCAGTCTAAGTCCACTGAATTGAATTTACAAAAATAAATAAAATCATTATTACTGTTTTCC

Fln Alignment:

Contig1288___MEAAEFATELESSKQQTNYAADISSIQEARDRIGPYIDKTPVLSSSSLNIIAGRTLFFKCEIFQKGGAFKFRGACNAVFSLPVDLADKGVVTHSSGNHXXXXXXXAKLRGIPAYIVIPQNAPICKVDNVKRYGGQVIWCEPTMQSRESVSAKVQEETGAVLIHPFNDGRIISGQGTISLELLEKVPQLDAIVVPISGGGLISGVALAAKALKPNILIFAAEPKGADDAARSKAAGHIVTLTETKTVADGLRAYLGELTWPXXXXXXXXXXXXXXXXXXEAMKMCYEILKLAVEPSGAIGLAAILSHQFQDNLRWSHCQNIGIVLSGGNVDLSVLWESFHKAH
A9NZE1______________MEAAKFSTELESSKQQTNYAADISSIQEARDRIGPYIDKTPVLSSSSLNIIAGRTLFFKCEIFQKGGAFKFRGACNAVFSLPVDLADKGVVTHSSGNHAAALALAAKLRGIPAYIVIPKNAPICKVENVKRYGGQVIWCEPTMQERESVSAKVQEETGAVLIHPFNDGRIISGQGTISLELLEQVAQLDAIVVPISGGGLISGVAVAAKALKPNILIFAAEPKGADDAARSKAAGHIVTLTETKTIADGLRAYLGDLTWPIVRDLVDEIIVVEEGEIVEAMKICYEILKLAVEPSGAIGLAAVLSHQFQDNMRWSHCQNIGIVLSGGNVDLSVLWESFQKAH

SNPs (tot: 17)

Position	A %	C %	G %	T %	Probability
319	75			25	0.996473
676	10	90			0.90298
677	10			90	0.90298
679		90	10		0.90298
680	90			10	0.90298
1046	11	88			0.923564
1048	11	88			0.923564
1317		50	50		1.0
1320			33	66	0.997452
1321			66	33	0.997452
1322	66	33			0.997452
1323	66		33		0.997452
1326	66	33			0.997452
1327	66			33	0.997452
1328		33	66		0.997452
1329		33		66	0.997452
1330	66		33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain