SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1276

Length: 1711 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene1276

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Motile_Sperm domain containing protein	-	-	1.0e-17	32%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	58%
Blast2go	vesicle-associated protein 4-2-like	-	-	0.0	73%

Source	GOs	Term	Type	Identity
Blast2go	response to gibberellin stimulus	GO:0009739	Biological Process	73%
Blast2go	structural molecule activity	GO:0005198	Molecular Function	73%
Blast2go	plastid	GO:0009536	Cellular Component	73%

Source	InterPros	Term	Type	Identity
Sma3	Major sperm protein	IPR000535	-	-
Sma3	Alcohol dehydrogenase, zinc-type, conserved site	IPR002328	-	-
Sma3	PapD-like	IPR008962	-	-
Sma3	Aldehyde dehydrogenase, conserved site	IPR016160	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NRI2

Fln msg:

Fln protein: MAAAVESGPSPSSENGTLTGCRLCALPWWQKRPAEGRQDVSENHSTRRPPVQNGQSLLASAKASLLTRRRLRLDPDKKLFFMYEPGKQASSVVKIRNVSRSSVAFKFQTNAPKSCFMKPPNGILGPKESITTTVVKFVEQPENSQERKKTKDKFKIVSLKVREGVEFTPELFDENKESLAVEKVLRVVFLDPQRPSKELEKLKKRLAEAEADSEARKKPVDDKAAKPPGPTEGVLDEWKVRRERYLARQAAEGADSV*

Protein Length: 259

Fln nts: TTTGAACCTAAATTTGTTTTGAATAATCCAATTTCTAGCGTTTTTTTCTTGGAAAGTGAAGTTAAAAACAGGCAAATCAATTTGTCCTTATTAGGGGTTAGGGAAATATTGATTTACGGTCAAATATCCACGAGGAGCATATGGACAACAATTTTCAAGCGTGTTCATGAATTTTTAGCGGTTATTGTTGAAGCAACTAGCCTGAATTTTTTAAAACAATGGATCACGAGTTTCGTGGAGAGTATCAGATAAAACTGGAAATGTTAGGCCTAAGGCCGTACAAGGCATGATCCAGGCGGTTTGAATTTTCGGCGTGTTTATTTGTATCTTTTGGCACCTGTTTGGCTACAAAGTGGTACACGAACTGAAATTTAAGGTAGGGAAGAGTTTCAAAGCTCAAGAGTTTGTAGATCGCATTTAGC_-_ATGGCCGCTGCCGTTGAGTCTGGTCCGAGTCCGAGCTCGGAAAATGGAACTTTAACAGGGTGCAGACTCTGTGCATTGCCGTGGTGGCAAAAAAGACCTGCGGAAGGGCGTCAGGATGTATCTGAGAATCACAGCACTCGGCGTCCCCCTGTTCAGAATGGGCAATCTTTATTAGCATCTGCAAAAGCGTCGCTGTTAACCAGGCGCCGCCTCCGACTTGATCCAGACAAAAAACTCTTCTTCATGTATGAACCTGGAAAGCAAGCTTCCAGCGTTGTGAAGATAAGGAATGTCAGCAGATCGTCTGTAGCCTTTAAATTCCAGACAAATGCTCCCAAAAGCTGTTTTATGAAACCTCCAAATGGTATACTTGGTCCAAAAGAGTCCATAACTACAACTGTTGTAAAATTTGTAGAGCAGCCAGAAAACTCTCAGGAAAGGAAGAAGACGAAGGATAAATTCAAAATTGTGAGCTTGAAAGTAAGAGAGGGCGTTGAATTTACTCCGGAACTGTTTGACGAGAATAAGGAGTCACTGGCAGTGGAGAAAGTATTGCGTGTTGTATTTCTTGATCCACAACGCCCATCCAAGGAGCTGGAGAAATTGAAGAAGCGTTTAGCAGAAGCAGAAGCTGATAGCGAAGCACGCAAGAAGCCTGTGGATGACAAGGCTGCAAAACCTCCTGGTCCTACAGAAGGCGTCCTAGATGAATGGAAGGTACGAAGAGAGAGATATCTGGCAAGACAAGCAGCTGAAGGAGCAGACTCTGTGTGA___ATATATTAGATTTTTCTGAGGGGAACAGCGTGTTTGTTCTGTTAGGAATCTTTTGGTGCTTATTTTCTTTGGAGGTTTAATAGCATACAGGGGAAAATGAATTTGGACATTGTAAAATGGATGCTACAGTACAACATCCTTTTGCTATCTGTCAACATATGCATTGCTGTTTGAGGAGAATCTTTCATGTACATATAGTTTGCCATTGCAAACTGAGGTTCCTATTTATTGTAAGTATTAATTCCACTGTACTAAACATTGCTCCACTATTGTTCTTTAGCTTTTATACTTCTGGAGGTATGCAATTCGACTCCATCCAAGCACGTTAGGACTCTGCATTCGTATTCAGTAATTGTATCTCAGAGGAAGGAGACGGTCGTCAAATTGAGTTGATATCAACCTTGACAGTTTTGATCGGATGACAGAGCCTCTACGTAGGCTCGCTATTTCTTTCTGCAACTGTTTGACATGAATTGAATATATTCTTGATTTCTTCAAATTCAATTTAGTTGTTA

Fln Alignment:

Contig1276___MAAAVESGPSPSSENGTLTGCRLCALPWWQKRPAEG---------RQDVSENHSTRRPPVQNGQXXXXXXXXXXXXXXXXXX-DPDKKLFFMYEPGKQASSVVKIRNVSRSSVAFKFQTNAPKSCFMKPPNGILGPKESITTTVVKFVEQPENSQERKKTKDKFKIVSLKVREGVEFTPELFDENKESLAVEKVLRVVFLDPQRPSXXXXXXXXXXXXXXXDSEARXXXXXXXXXXXXXXTEGVLDEWKVRRERYLARQAAEGADSV
A9NRI2______________MMATADFGGSTAQRSSK--GWNLCPMPWLNNGGSSSNVNSGNNSNRGSVDRNPNSNRHPLNTKQMSLGAIAKASFLVRRRLRFDPDRKLYFIYEPGKQVSSAVRIKNVSRSYVAFKFQTNAPKSCFMRPPNGILAPKESILATVMKFVEQPEHPNE-KKTKDKFRIVSLKVKEGAEFTSELFDEHKELVAVERVLQVVFLDPQRPCQDLEKLKKRLAEAEALNQARKKPQDDNTPKSTDPAEGVLDIWKDRREKYLARQQVE--DSV

SNPs (tot: 17)

Position	A %	C %	G %	T %	Probability
763	10		90		0.90298
773	11		88		0.923564
778		88		11	0.923564
781	11	88			0.923564
789	88			11	0.923564
882	83		16		0.994512
886	83		16		0.994512
887	83		16		0.994512
1047	83			16	0.994512
1049		83	16		0.994512
1050	83		16		0.994512
1088	85		14		0.993533
1089		85	14		0.993533
1207	50			16	1.0
1208	50			50	1.0
1360	16		83		0.994512
1616	10		90		0.90298

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain