SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1231

Length: 1550 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene1231

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	putative mitogen-activated protein kinase 1 [Medicago sativa]	-	-	63%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	49%
Blast2go	mitogen-activated protein kinase kinase kinase 1-like	-	-	65%

Source	GOs	Term	Type	e value	Identity
Blast2go	protein kinase activity	GO:0004672	Molecular Function	0.0	65%
Blast2go	nucleotide binding	GO:0000166	Molecular Function	0.0	65%

Source	InterPros	Term	Type	Identity
Sma3	Armadillo	IPR000225	-	-
Sma3	HECT	IPR000569	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	NB-ARC	IPR002182	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	AAA+ ATPase domain	IPR003593	-	-
Sma3	DNA-binding WRKY	IPR003657	-	-
Sma3	Paired amphipathic helix	IPR003822	-	-
Sma3	Ribosomal protein S12/S23	IPR006032	-	-
Sma3	Epidermal growth factor-like	IPR006210	-	-
Sma3	Twin-arginine translocation pathway, signal sequence	IPR006311	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Leucine-rich repeat 3	IPR011713	-	-
Sma3	Armadillo-like helical	IPR011989	-	-
Sma3		IPR015748	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	Transcription regulator, HTH DeoR-type, conserved site	IPR018356	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: B8LR19

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 190 aas, your sequence is shorter than subject: 516 - 902

Fln protein: SDWEIRKTKSRCSSLGNEGISQEIKDLSREIEGQTHNIRSLSPPEVSSPKGNGSAAVSPPLLSETPSVPASDSPGESEVGGPEISGLEGGRKLVVSVRTRGDHCRIKPLSEAAATSACVQSFTLAPPPSMSLKVSNDKGASTWDILKSLAPEEDGICLPSTDRALTPNSQESDDGSDSDEEFEEEKQEDDRGAEVKRGRSWVLSSSSSGGESFTSSTEKAVYTVSKDGKFKVRFDSWIKGNMLGRGSFGTVYEGISDTGFFFAVKEVSLVEQGLGAKQCISQLEQEIALLSQLEHKNIVQYLGTDQEPEKLYIFLELVTQGSLQSLYQKYRLIDFQARAYTRQILIGLKYLHDRNVIHRDIKCANILVDTHGVIKLADFGMAKEISKLNELKSCKGSAYWMAPEVINPRKSYWLPADIWSLGCTVLEMLTRRPPNGDLEWHRALWKVGHGEIPPIPNALSSDAKDFIKRCLKIEAADRPSAAELLDHPFVKRSLSADVSTAEPSTKSRRQRPLAR*

Protein Length: 517

Fln nts: CCTCCGATTGGGAAATTCGAAAGACTAAATCGAGATGCAGCTCTTTGGGAAATGAAGGGATTTCTCAGGAAATTAAAGACCTTTCTCGGGAAATTGAAGGGCAGACGCACAACATTAGGTCATTGTCTCCCCCAGAAGTCTCCAGCCCCAAAGGAAACGGGTCCGCTGCCGTGTCCCCGCCTTTGCTCTCAGAGACTCCGTCTGTGCCGGCATCCGATAGCCCCGGAGAGAGCGAAGTCGGGGGTCCTGAAATAAGCGGGTTGGAGGGTGGCCGCAAGCTTGTGGTTTCGGTTCGCACAAGAGGAGACCATTGTAGGATTAAACCCCTTTCTGAGGCCGCGGCTACTTCGGCTTGTGTCCAATCGTTTACCCTGGCTCCGCCGCCGTCGATGTCTTTGAAGGTTTCGAATGACAAGGGTGCTTCGACTTGGGATATTTTGAAGTCTTTGGCTCCCGAGGAGGATGGCATTTGTTTGCCTTCAACTGACAGGGCTTTGACGCCAAATTCGCAGGAGAGTGATGATGGTAGTGACAGTGATGAGGAATTTGAGGAGGAAAAGCAAGAGGATGATAGGGGTGCAGAGGTGAAAAGGGGCAGATCGTGGGTATTGTCGTCGAGCTCAAGCGGAGGTGAGTCATTTACGTCGTCAACAGAGAAGGCCGTCTATACCGTTTCCAAGGATGGGAAATTCAAGGTTCGGTTCGATTCTTGGATTAAAGGGAATATGCTCGGGCGGGGATCATTTGGAACTGTTTACGAGGGAATTAGCGATACTGGATTCTTCTTTGCAGTTAAGGAGGTTTCTCTTGTAGAGCAAGGTCTTGGTGCAAAACAGTGCATTTCTCAGCTGGAACAGGAGATTGCTCTTCTAAGTCAACTTGAACATAAAAACATCGTTCAGTATCTTGGCACCGACCAGGAACCTGAAAAGTTGTACATTTTCCTTGAGCTGGTAACACAGGGTTCACTACAATCACTTTACCAAAAATACCGCTTAATTGATTTTCAAGCTCGTGCATATACAAGACAAATACTAATTGGACTAAAGTACCTTCACGATAGAAATGTCATTCACAGAGACATTAAGTGCGCTAATATATTGGTGGATACCCATGGCGTAATCAAACTTGCAGATTTTGGGATGGCCAAAGAGATATCCAAGTTGAATGAATTAAAGTCGTGCAAAGGAAGTGCTTATTGGATGGCTCCTGAGGTTATTAATCCTCGAAAATCATACTGGCTTCCTGCTGACATATGGAGCTTAGGTTGCACTGTGTTGGAGATGCTAACTCGGAGACCTCCCAATGGTGATTTAGAATGGCATCGAGCCTTGTGGAAAGTTGGACACGGGGAGATACCACCAATACCGAATGCACTATCTAGTGATGCCAAGGACTTTATAAAACGTTGTTTAAAAATTGAGGCTGCAGATCGACCATCAGCAGCCGAGCTCCTGGATCATCCTTTTGTGAAACGGTCACTTTCAGCAGACGTGTCCACTGCTGAGCCTTCCACTAAAAGTAGACGCCAGAGACCTCTAGCAAGATGA

Fln Alignment:

Contig1231___WIKGNMLGRGSFGTVYEGIS-DTGFFFAVKEVSLVEQGLGAKQCISQLEQEIALLSQLEHKNIVQYLGTDQEPEKLYIFLELVTQGSLQSLYQKYRLIDFQA-RAYTRQILIGLKYLHDRNVIHRDIKCANILVDTHGVIKLADFGMAKEISKLNELKSCKGSAYWMAPEVINPRKS-YWLPADIWSLGCTVLEMLTRRPPNGDLEWHRALWKVGHGEIPPIPNALSSDAKDFIKRCLKIEAADRPSAAELLDHPFVKRSLSADVSTAEPSTKS
B8LR19______________WQKGKLLGCGTFGTVYVGFNRETGDMCAMKEVPLVPDDSKSSESIKQLEQEINLLSGLEHPNIVQYYGSETVEDLFYIYLEYVPGGSIYKLVNDYGPLEEPVIRIYTRQILSGLAYLHSMNTVHRDIKGGNILVDTYGRVKLADFGMAKHINGPATPLSLKGSPYWMAPEVLMQKNTGHDLAVDIWSLGCTVIEMATGKPPWSEYEGAAAMFKVFKSEVPPIPDSLSPEGRHFVQCCLCRNPAQRPKASQLLEHLFVRNATQQDSSDLLASATS

SNPs (tot: 44)

Position	A %	C %	G %	T %	Probability
372		80	20		0.995493
423		50	50		1.0
451	12		87		0.992554
531	14		85		0.993533
534	85			14	0.993533
544		14	85		0.993533
639	14	85			0.993533
642			14	85	0.993533
707	16		83		0.994512
718			16	83	0.994512
725	80		20		0.995493
727	20			80	0.995493
729			20	80	0.995493
730	20		80		0.995493
738	20		80		0.995493
761	25		75		0.996473
767	75			25	0.996473
825			33	66	1.0
827		33	66		1.0
926		66		33	1.0
991	60			40	1.0
1008		20		80	0.995493
1012	60			40	1.0
1014		80		20	0.995493
1028	80		20		0.995493
1041		28		71	1.0
1067	66		33		1.0
1075			33	66	1.0
1095		71	28		1.0
1112	57		42		1.0
1121		28	71		1.0
1127	85		14		0.993533
1145	87			12	0.992554
1158		12		87	0.992554
1185			83	16	0.994512
1187	83	16			0.994512
1194		16	83		0.994512
1197		83		16	0.994512
1230	16		83		0.994512
1242		14	85		0.993533
1243			85	14	0.993533
1329		88	11		0.923564
1330			88	11	0.923564
1467	33			66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain