SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1227

Length: 1838 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene1227 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	putative 1,3-beta glucanase [Oryza sativa Japonica Group] dbj\|BAD10703.1\| putative 1,3-beta glucanase [Oryza sativa Japonica Group]	-	-	59%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	60%
Blast2go	glucan -beta-glucosidase	-	-	71%

Source	ECs	Term	Type	e value	Identity
Blast2go	Hydrolases, Glycosylases, Glycosidases, i.e. enzymes hydrolyzing O- and S-glycosyl compounds.	EC:3.2.1.-	-	0.0	71%

Source	KEGGs	Term	Identity
Blast2go	Ascorbate and aldarate metabolism	00053	71%
Blast2go	Ubiquinone and other terpenoid-quinone biosynthesis	00130	71%
Blast2go	Amino sugar and nucleotide sugar metabolism	00520	71%
Blast2go	Glycosaminoglycan degradation	00531	71%
Blast2go	Metabolic pathways	01100	71%

Source	GOs	Term	Type	Identity
Blast2go	carbohydrate metabolic process	GO:0005975	Biological Process	71%
Blast2go	hydrolase activity, hydrolyzing O-glycosyl compounds	GO:0004553	Molecular Function	71%
Blast2go	protein binding	GO:0005515	Molecular Function	71%
Blast2go	cation binding	GO:0043169	Molecular Function	71%

Source	InterPros	Term	Type	Identity
Sma3	Glycoside hydrolase, family 5	IPR001547	-	-
Sma3	Beta-hydroxyacyl-(acyl-carrier-protein) dehydratase, FabA/FabZ	IPR013114	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-
Sma3	Glycoside hydrolase, family 5, conserved site	IPR018087	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LKR6

Fln msg: Separated hits, possible frame ERROR between 159 and 164, your sequence is shorter than subject: 450 - 511

Fln protein: QNAVVNRDNASTWETFKMWRVSDGMYQLRVFSGQFLTAENGGGGNVSAEADSxxxDWETFHIIRHHKHPNRVHIRVSNGMYMQAQSQEQLTADYQGDPGWHDNSATFQMIIVANDLHGDYQLANGYGRDKSKKVYHRHRSSFITDKDFKFLSRNGINTVRIPVGWWIAYDPNPPAPFVGGSLQSLDNAFKWADHYGLKVIVDLHAAPGSQNGMEHSASRDGSADWSNSSDHISQSLTVIDFLVSRYSNNSMLLGIELLNEPLATTVPVDVLASYYSNGYQTVRKYSATTYVIMCQRIGNADPHELYQINDAFSNIVVDVHYYNLFDDYFDNKTAQDNIDFILKNREPQVEALNSTNGPLIFVGEWTNEWKVQGASRSEYQQFGDAQLQAYEKASFGWSYWTLKNEEKHWDFEWNIKHNYLRLGNSRATSNKSISWRIYAFLLIFGCIYV*

Protein Length: 451

Fln nts: CAGAATGCTGTTGTTAATAGGGACAATGCTTCTACCTGGGAGACTTTCAAGATGTGGAGAGTCTCAGATGGCATGTATCAACTTAGAGTGTTTAGTGGTCAATTCCTAACAGCAGAAAATGGAGGAGGAGGCAACGTGTCTGCAGAAGCAGATTCTCCxxxxxxTGATTGGGAAACATTCCACATAATTAGGCACCATAAACATCCTAACCGAGTGCATATCAGGGTGTCCAATGGAATGTATATGCAGGCACAATCCCAGGAGCAGCTTACAGCAGATTACCAAGGGGATCCTGGGTGGCATGACAATTCAGCAACCTTCCAGATGATTATAGTTGCCAATGATTTGCATGGAGATTACCAGCTTGCAAATGGATATGGCCGTGATAAATCAAAGAAAGTTTACCATAGACATAGAAGCAGCTTTATTACAGATAAGGATTTCAAATTCCTCTCCAGGAATGGTATAAATACCGTTAGAATTCCTGTTGGGTGGTGGATTGCATATGATCCGAATCCACCAGCACCTTTCGTAGGAGGATCTTTACAATCTCTCGATAATGCTTTTAAATGGGCAGATCATTATGGTTTAAAGGTTATTGTTGACCTCCATGCGGCACCTGGTTCACAAAATGGAATGGAGCATAGTGCCAGCAGAGATGGATCTGCTGACTGGTCTAACTCTTCAGACCATATTTCCCAAAGTTTAACTGTTATTGATTTCCTTGTTTCGAGATATAGTAACAATAGTATGCTCTTAGGTATTGAATTGCTGAATGAACCTCTAGCAACAACTGTTCCTGTGGATGTTTTGGCTTCCTATTATTCAAATGGCTATCAAACTGTGCGGAAGTACTCTGCCACAACATATGTTATCATGTGCCAGAGGATTGGAAATGCTGATCCACATGAGCTGTATCAAATAAATGATGCATTTTCAAATATCGTTGTTGATGTTCACTACTATAATCTTTTTGATGATTATTTTGATAACAAGACTGCCCAAGATAATATTGACTTCATCTTGAAGAACAGGGAACCCCAAGTGGAAGCTCTAAACAGCACAAATGGTCCTCTCATATTTGTCGGTGAATGGACAAATGAGTGGAAGGTGCAAGGAGCTTCAAGGTCGGAATATCAACAGTTTGGTGATGCACAATTACAAGCTTATGAAAAGGCTTCATTTGGTTGGTCATATTGGACTCTAAAGAACGAAGAGAAGCACTGGGATTTTGAGTGGAATATCAAGCACAACTACCTTAGATTAGGTAATTCGAGGGCAACATCAAACAAGAGTATATCTTGGAGAATATATGCCTTTTTGTTGATCTTTGGGTGTATTTATGTATGAGACTTGAGTAGCCGGGCAATCTGACACTTTAATGCTGCTAAAAAATAGCTGGTGGTTCTTTCTGAAATAGATTTGACATATATGATGGGACAATTCCTACATTTTAATCCACTGAAGGGCAACAGTGATTGGTACTTGCAGTGATTCCATGATTCAATTCTTGCTCTTTTTCCAGAGCATAGAATTATATCAACCTCCTGAAGTGTGGAATAAACTTATGACAGAGGACATCACCAGGTTTAAACAGAATCTAAGCAATTTAAAGAGAATTAACATGTGAGATGGTACAGAATCCATACTGTGGACCTCCGCAGAAATGGCAGATTCTATATAAGCAACTGCAAAGGTGGCAAAACCTTGTAATAAGAAATGACCTGGGTTGTAAACGTACATTAAATACTGTTAACTGCATTCACTTCAAGGAATTGACGTCGGATTCATTCTTTGTTTTTGCTGTTCAATTTATCCAACCACTTGAACCTTGTATACG

Fln Alignment:

Contig1227___QNAVVNRDNASTWETFKMWRVSDGMYQLRVFSGQFLTAENGGGGNVSAEADSPxxDWETFHIIRHHKHPNRVHIRVSNGMYMQAQSQEQLTADYQGDPGWHD-NSATFQMIIVAND--LHGDYQLANGYGRDKSKKVYHRHRSSFITDKDFKFLSRNGINTVRIPVGWWIAYDPNPPAPFVGGSLQSLDNAFKWADHYGLKVIVDLHAAPGSQNGMEHSASRDGSADWSNSSDHISQSLTVIDFLVSRYSNNSMLLGIELLNEPLATT------VPVDVLASYYSNGYQTVRKYSATTYVIMCQRIGNADPHELYQINDAFSNIVVDVHYYNLFDD-YFDNKTAQDNIDFILKNREPQVEALNSTNGPLIFVGEWTNEWKVQGASRSEYQQFGDAQLQAYEKASFGWSYWTLKNEEKHWDFEWNIKHNYLRL
B8LKR6______________KNLVVNRGSASGWETFRVWRVKDGTYQFRAFNKQFVRAVNAGGGIVDATADTPxxEWETFQIIRNPSNKNQVHIKAYNGMYIQAQSQDQLTADFQGEPGWNDGNAATFEMNIVTGDYGIGGEYQLTNGLGPDKAPQVMNDHWNSFITESDFEFMSSKGITAVRIPVGWWIASDPNPPAPFVGGSLKALDNAFTWAKNHNIGVIVDLHAVPGSQNGDAHSGTRDGYLEWPNSQDNIDKSISVIDFLAGRYASNSALLGIELLNEPRASTDPNAPGVPLDTLKTYYKRGYDTIRKHSPSAYVIMCNRIGPADAKELFDINNGLTRTVVDVHYYNLYDDSTFKSMSVQQNIDYINNNRAQILQSLTSANGPLIYVGEWTNEWEYQGASMSDYQRFGKAQLQVYGTATFGWSYWTLKNVQNHWSFEWMVQNNYLQL

SNPs (tot: 36)

Position	A %	C %	G %	T %	Probability
843	12		87		0.992554
845		87	12		0.992554
890	75		25		1.0
920			16	83	0.994512
924		12		87	0.992554
929		87	12		0.992554
933		11		88	0.923564
935	11	11		77	0.968132
936		11	88		0.923564
940	11	88			0.923564
943		88		11	0.923564
958	10		90		0.90298
959	10		90		0.90298
960		90		10	0.90298
961	90		10		0.90298
962		90		10	0.90298
1031	87		12		0.992554
1125	85			14	0.993533
1187	20		80		0.995493
1190			80	20	0.995493
1271	33			66	0.997452
1273			33	66	0.997452
1274	66			33	0.997452
1350		85	14		0.993533
1353	85	14			0.993533
1448	40			60	1.0
1449		40	60		1.0
1450	40	40		20	1.0
1686	20		80		0.995493
1687			80	20	0.995493
1688	80		20		0.995493
1689			80	20	0.995493
1758		25		75	0.996473
1793	25			75	0.996473
1800	66	33			0.997452
1802			66	33	0.997452

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain