SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1177

Length: 2448 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene1177
ATGGGAGCAAATAAAAAGAGAACCTTTTCCTTTCTGCACACATCCCAGAGGAGGTTGACAACCCCATTAGCTTTGAGTGGTCATGTTTTTCTGTTTGACAAACAGGTGCCTCACTGGTTGTTCCTTCACCTTCAGGTTTGAATGTCCTTGTTGTTCGAAATGGGGACTCCAGCTCAAAAGATGCATCTCCTCCAGTACAACTTGAAATTTGGGGTCCTGGTCAGTTGCGAAAAGAGATATTTGTTCCTCCGTCAGTGCATGGTCCTTTGTATACAGATGGATGGTTTGAAGGTGTTTCATGGAGTTCAGATGAGACTTGTATAGCTTATATTGCAGAGGAACCAGCAACTCCTCAACCTATATTTACCAGTTCTGGGTGCAAGCCAGTAAGTTCAGGTGAAAAGGAGCCAGGAAGTTGGAAAGGTCAAGGAGATTGGTTGGAGGATTGGGGGGAAACCTACTCAGGAAAAAGGAGACCAGCTCTATTTGTTGTCAACATTAAAAGTGGGACTGTGCAGGTTGTTGAAGGCATCCCGGACAATTTAAGTGTTGGACAAGTGGTGTGGGCACCACATGTTTCCACTAATAATTCTGGGTATCTAGTTTTTGTTGGATGGTCAGTAGATGCTGGTGATTCCCAAAACCCCAGAAAGTTAGGAATGAAATACTGTTACAATAGGCCGTGTGCTCTGTATTCAGTTAAAGCACCATTGCCAAAATTAGATTCCTCTAAGGAAATGATAGCAGCACAGAAGCTCACAGAGAAAATTGACAGTGCTTTCTTACCACGGTTCAGTCCAGATGGGAAGCTGTTAGTCTTTTTGTCTGCAAGCACTGCCGTCAATACTGGAGCCCATTGTGTTACCAATTCACTCCACAGTTTTCATTGGCCCTCTGATGGGACAGTGCATCCAAACCTTGAAATAAATGATGTGGTCTCTCTTGTGATGTGCCCAAGGGAGGGTTGTTTTCCAGGATTATACTGCACAAATGTCCTGTTTCATCCATGGCTTTCTGATGGGTGTACCTTGGTTCTATCCTCAATTTGGGGTAGCATGCAAGTTTTACTTGCAGTAAATGTAGTCAGTGGAAAGGTTTCAAGAATTAGCCCTGAGAATTCAAATTCTTCTTGGAGTGTTCTTGCAGTGCATGATAACATTGTTCTTGCAGTGTCTAGCAGCCCAGCCAATCCTCCGAAACTGAAATATGGCCATCTGTCAAGAATTGACACGGATAATGATGAAAAAACAAGGTGGAGGTGGTTAGATATGCCAATTCCCTTCATGGACTATTCAGAGAAAGTGAAAGCTTCGTTGTCCTCAATTGAATTTAATATTTTGAAGATTCCGGTTAAAGATCAAGAATTTTTGTTAACAGAAGGTGCCAAACAGCCTTTCGAGGTGATATTTGTGTCATCTAGATTCTCCAGTGAATCAGAAAGTAAGGAAAATGCTTTTCGGGAGGATGCTTGTAATCCACTCATTGTTATCCTCCATGGTGGGCCACATTCTACTTCATTGACAAGCTTTTCAAGAACTCAAGCCTTTTTGTCATCAATGGGCTACAACCTACTTTATGTAAACTACAGAGGTTCTTTGGGCTTTGGAGAGGAGGCTTTGCAATCTCTACCTGGTAATGTTGGGCGACAGGATGTGGCTGATGTCATAAATGCTGTTGATCTAGTAATTCACAAGGGACTTGCCCTCCCAACAAACATAGTAGTGCTTGGTGGGTCACACGGAGGGTTTCTTGCAACCCATTTAATTGGCCAGGCACCAGATAGGTTTGTTGCTGCAGCTGTCCGGAATCCTGTGTGTAATCTCTCATTAATGGTTGGCACAACAGATATTCCTGATTGGTGTTATGTGGAGGCATATGGAAAGAATGGGAAAAATGTTTACACTGAAGCACCTTCAATAGAAGATATCACCATGTTTTATCAGTGTTCACCAATTAGACATATTTCAAAGGTAAAAGTACCCGTTCTCTTACTTTTGGGTGCGCAAGACCTCAGGGTTCCTGTATCAAATGGATTGCAGTATGCTCGAGCTCTGCGGGAGAAGGGGGTGGAAGTAAAAGTCATCGTTTTCCCAAATGATGTACACGGCATTGACAGGCCGCAATCTGATTTCGAAAGCTTTCTGAACATTGGACTTTGGTTTAAGAGATTCTTGAAATGAAGGCCTTTGGCGGGCCCATGTTCCTCTATTGGTTACGTGCTTGTTTTGTTCAGTAAGATTGTGGATATTAACAAAAAGCATTATATATAGACTACAATATAGGGCCTAATGAAGCTTTAATATAAAGCCTTCATTTCTCCTCAGATGCATGTTCAATTATATAGATTTATAAAACTGATTTTATGATGCATTTTATGTCAATATATTAAAAGGGCTCAATGAGAAGATTAAACTTGCCAATAAATTAATTTATTTTGTTTTGATGTAC

Ace file of the UniGene sp_v2.0_unigene1177 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	acylaminoacyl-peptidase [Arabidopsis thaliana] pir\|\|JC8016 acylaminoacyl-peptidase (EC 3.4.19.1) - Arabidopsis thaliana dbj\|BAC76411.1\| acylamino acid-releasing enzyme [Arabidopsis thaliana] gb\|AEE83461.1\| acylaminoacyl-peptidase [Arabidopsis thaliana]	-	-	55%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	27%
Blast2go	acylamino-acid-releasing enzyme	-	-	70%

Source	GOs	Term	Type	Identity
Blast2go	proteolysis	GO:0006508	Biological Process	70%
Blast2go	serine-type aminopeptidase activity	GO:0070009	Molecular Function	70%
Blast2go	vacuole	GO:0005773	Cellular Component	70%
Blast2go	chloroplast	GO:0009507	Cellular Component	70%

Source	InterPros	Term	Type	Identity
Sma3	Peptidase S9, prolyl oligopeptidase, catalytic domain	IPR001375	-	-
Sma3	Peptidase aspartic, active site	IPR001969	-	-
Sma3	Peptidase S9A, prolyl oligopeptidase	IPR002470	-	-
Sma3	Peptidase S9, serine active site	IPR002471	-	-
Sma3	WD40-like Beta Propeller	IPR011659	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NWZ2

Fln msg: query STOP codon is far from subject stop. Distance to subject end: 17 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 507 - 721

Fln protein: MKYCYNRPCALYSVKAPLPKLDSSKEMIAAQKLTEKIDSAFLPRFSPDGKLLVFLSASTAVNTGAHCVTNSLHSFHWPSDGTVHPNLEINDVVSLVMCPREGCFPGLYCTNVLFHPWLSDGCTLVLSSIWGSMQVLLAVNVVSGKVSRISPENSNSSWSVLAVHDNIVLAVSSSPANPPKLKYGHLSRIDTDNDEKTRWRWLDMPIPFMDYSEKVKASLSSIEFNILKIPVKDQEFLLTEGAKQPFEVIFVSSRFSSESESKENAFREDACNPLIVILHGGPHSTSLTSFSRTQAFLSSMGYNLLYVNYRGSLGFGEEALQSLPGNVGRQDVADVINAVDLVIHKGLALPTNIVVLGGSHGGFLATHLIGQAPDRFVAAAVRNPVCNLSLMVGTTDIPDWCYVEAYGKNGKNVYTEAPSIEDITMFYQCSPIRHISKVKVPVLLLLGAQDLRVPVSNGLQYARALREKGVEVKVIVFPNDVHGIDRPQSDFESFLNIGLWFKRFLK*

Protein Length: 508

Fln nts: ATGGGAGCAAATAAAAAGAGAACCTTTTCCTTTCTGCACACATCCCAGAGGAGGTTGACAACCCCATTAGCTTTGAGTGGTCATGTTTTTCTGTTTGACAAACAGGTGCCTCACTGGTTGTTCCTTCACCTTCAGGTTTGAATGTCCTTGTTGTTCGAAATGGGGACTCCAGCTCAAAAGATGCATCTCCTCCAGTACAACTTGAAATTTGGGGTCCTGGTCAGTTGCGAAAAGAGATATTTGTTCCTCCGTCAGTGCATGGTCCTTTGTATACAGATGGATGGTTTGAAGGTGTTTCATGGAGTTCAGATGAGACTTGTATAGCTTATATTGCAGAGGAACCAGCAACTCCTCAACCTATATTTACCAGTTCTGGGTGCAAGCCAGTAAGTTCAGGTGAAAAGGAGCCAGGAAGTTGGAAAGGTCAAGGAGATTGGTTGGAGGATTGGGGGGAAACCTACTCAGGAAAAAGGAGACCAGCTCTATTTGTTGTCAACATTAAAAGTGGGACTGTGCAGGTTGTTGAAGGCATCCCGGACAATTTAAGTGTTGGACAAGTGGTGTGGGCACCACATGTTTCCACTAATAATTCTGGGTATCTAGTTTTTGTTGGATGGTCAGTAGATGCTGGTGATTCCCAAAACCCCAGAAAGTTAGGA_-_ATGAAATACTGTTACAATAGGCCGTGTGCTCTGTATTCAGTTAAAGCACCATTGCCAAAATTAGATTCCTCTAAGGAAATGATAGCAGCACAGAAGCTCACAGAGAAAATTGACAGTGCTTTCTTACCACGGTTCAGTCCAGATGGGAAGCTGTTAGTCTTTTTGTCTGCAAGCACTGCCGTCAATACTGGAGCCCATTGTGTTACCAATTCACTCCACAGTTTTCATTGGCCCTCTGATGGGACAGTGCATCCAAACCTTGAAATAAATGATGTGGTCTCTCTTGTGATGTGCCCAAGGGAGGGTTGTTTTCCAGGATTATACTGCACAAATGTCCTGTTTCATCCATGGCTTTCTGATGGGTGTACCTTGGTTCTATCCTCAATTTGGGGTAGCATGCAAGTTTTACTTGCAGTAAATGTAGTCAGTGGAAAGGTTTCAAGAATTAGCCCTGAGAATTCAAATTCTTCTTGGAGTGTTCTTGCAGTGCATGATAACATTGTTCTTGCAGTGTCTAGCAGCCCAGCCAATCCTCCGAAACTGAAATATGGCCATCTGTCAAGAATTGACACGGATAATGATGAAAAAACAAGGTGGAGGTGGTTAGATATGCCAATTCCCTTCATGGACTATTCAGAGAAAGTGAAAGCTTCGTTGTCCTCAATTGAATTTAATATTTTGAAGATTCCGGTTAAAGATCAAGAATTTTTGTTAACAGAAGGTGCCAAACAGCCTTTCGAGGTGATATTTGTGTCATCTAGATTCTCCAGTGAATCAGAAAGTAAGGAAAATGCTTTTCGGGAGGATGCTTGTAATCCACTCATTGTTATCCTCCATGGTGGGCCACATTCTACTTCATTGACAAGCTTTTCAAGAACTCAAGCCTTTTTGTCATCAATGGGCTACAACCTACTTTATGTAAACTACAGAGGTTCTTTGGGCTTTGGAGAGGAGGCTTTGCAATCTCTACCTGGTAATGTTGGGCGACAGGATGTGGCTGATGTCATAAATGCTGTTGATCTAGTAATTCACAAGGGACTTGCCCTCCCAACAAACATAGTAGTGCTTGGTGGGTCACACGGAGGGTTTCTTGCAACCCATTTAATTGGCCAGGCACCAGATAGGTTTGTTGCTGCAGCTGTCCGGAATCCTGTGTGTAATCTCTCATTAATGGTTGGCACAACAGATATTCCTGATTGGTGTTATGTGGAGGCATATGGAAAGAATGGGAAAAATGTTTACACTGAAGCACCTTCAATAGAAGATATCACCATGTTTTATCAGTGTTCACCAATTAGACATATTTCAAAGGTAAAAGTACCCGTTCTCTTACTTTTGGGTGCGCAAGACCTCAGGGTTCCTGTATCAAATGGATTGCAGTATGCTCGAGCTCTGCGGGAGAAGGGGGTGGAAGTAAAAGTCATCGTTTTCCCAAATGATGTACACGGCATTGACAGGCCGCAATCTGATTTCGAAAGCTTTCTGAACATTGGACTTTGGTTTAAGAGATTCTTGAAATGAAGGCCTTTGGCGGGCCCATGTTCCTCTATTGGTTACGTGCTTGTTTTGTTCAGTAAGATTGTGGATATTAACAAAAAGCATTATATATAGACTACAATATAGGGCCTAATGAAGCTTTAATATAAAGCCTTCATTTCTCCTCAGATGCATGTTCAATTATATAGATTTATAAAACTGATTTTATGATGCATTTTATGTCAATATATTAAAAGGGCTCAATGAGAAGATTAAACTTGCCAATAAATTAATTTATTTTGTTTTGATGTAC

Fln Alignment:

Contig1177___PLIVILHGGPHSTSLTSFSRTQAFLSSMGYNLLYVNYRGSLGFGEEALQSLPGNVGRQDVADVINAVDLVIHKGLALPTNIVVLGGSHGGFLATHLIGQAPDRFVAAAVRNPVCNLSLMVGTTDIPDWCYVEAYGKNGKNVYTEAPSIEDITMFYQCSPIRHISKVKVPVLLLLGAQDLRVPVSNGLQYARALREKGVEVKVIVFPNDVHGIDRPQS
A9NWZ2______________PLLVKSHGGPTSESHSALDLSIQYWTSRGWAFADVNYGGSTGYGREYRERLNGSWGIVDVNDCCSCAEFLVTTGRVDGERLCITGRSAGGYTTLAAL-VFRETFKAGASLFGVADVSLLKADTH-----KFESY-------YTDSLVGKDESLLYERSPINFVDRLSCPMILFQGLEDKVVPPEQARKIYAAVKEKGLPVALVEYEGEQHGFRKAEN

SNPs (tot: 46)

Position	A %	C %	G %	T %	Probability
2185	10		90		0.90298
2189		90	10		0.90298
2193	10	90			0.90298
2194	90			10	0.90298
2195		90		10	0.90298
2223		66		33	1.0
2250	11			88	0.923564
2253		11		88	0.923564
2257		11	88		0.923564
2258		11	88		0.923564
2259	33	11	55		1.0
2260		11	88		0.923564
2261		11	88		0.923564
2264	77		11		0.968132
2266	11		88		0.923564
2269	11			88	0.923564
2277	11		88		0.923564
2278	11	88			0.923564
2283			37	62	1.0
2302	87			12	0.992554
2303			87	12	0.992554
2347	16		83		0.994512
2349	16			83	0.994512
2350	16			83	0.994512
2353		20		80	0.995493
2354		40		60	1.0
2355	40	60			1.0
2358	60	40			1.0
2359	20	60		20	0.99255
2360	60		40		1.0
2363	25		75		0.996473
2366	75	25			0.996473
2367	75	25			0.996473
2368		25	75		0.996473
2369	25		75		0.996473
2372	75	25			0.996473
2373		75		25	0.996473
2374		75		25	0.996473
2375	75			25	0.996473
2376		25	75		0.996473
2378		25	75		0.996473
2379	25		75		0.996473
2380	33	66			0.997452
2381	66			33	0.997452
2383		33		66	0.997452
2385	66			33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain