SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene1125

Length: 1578 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene1125 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	regulator of G-protein signaling 1 [Arabidopsis thaliana] gb\|AAN12971.1\| unknown protein [Arabidopsis thaliana] gb\|AEE77116.1\| regulator of G-protein signaling 1 [Arabidopsis thaliana]	-	-	52%
FL-Next	tr=Putative uncharacterized protein; Vitis vinifera (Grape).	-	-	55%
Blast2go	regulator of g-protein signaling 1	-	-	71%

Source	GOs	Term	Type	Identity
Blast2go	response to glucose stimulus	GO:0009749	Biological Process	71%
Blast2go	response to water deprivation	GO:0009414	Biological Process	71%
Blast2go	G-protein coupled receptor signaling pathway	GO:0007186	Biological Process	71%
Blast2go	response to abscisic acid stimulus	GO:0009737	Biological Process	71%
Blast2go	positive regulation of GTPase activity	GO:0043547	Biological Process	71%
Blast2go	regulation of cell proliferation	GO:0042127	Biological Process	71%
Blast2go	sugar mediated signaling pathway	GO:0010182	Biological Process	71%
Blast2go	G-protein coupled receptor activity	GO:0004930	Molecular Function	71%
Blast2go	GTPase activator activity	GO:0005096	Molecular Function	71%
Blast2go	nucleus	GO:0005634	Cellular Component	71%
Blast2go	plasma membrane	GO:0005886	Cellular Component	71%

Source	InterPros	Term	Type	e value	Identity
Sma3	Regulator of G protein signalling	IPR000342	-	0.0	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: tr_plants

Fln subject: D7TAJ5

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 47 aas, putative chimeric sequence, your sequence is shorter than subject: 339 - 466

Fln protein: MSCYLWTVWLEGPCGFGLLMSCRRALAYRLYYLFVKRRLPLIKSYQLILLILLPWFGAAAFSQAMRPFNHVCHVGKQWVIPVASLHVLYLLVLMGVTWVVSLVVFQFDEFKELSKGVTITLIFIGVWVALYVANEVHDEKSTRLMVASRFMLMLAANILVLSFFFISMSRPLFTQLSMRKRESDKLETMGRILGVPESGLLFPTGMPLNLDEPLEKLLCQKKFRQSFMAFADSRMAGESVHFYDEVHELNKIPIGDTARRIYMAGHIIEQYIAVDSPMEINISHQMRQEILDTTDLAHPDLFTNAVNEMVRMMQMNLEKDVPEFHFLFTIQGRDQRCS*

Protein Length: 340

Fln nts: AATGTCATGTTATCTTTGGACAGTATGGCTAGAAGGCCCATGCGGTTTTGGTTTGCTGATGAGCTGCCGTCGTGCACTAGCTTATCGACTTTATTATTTGTTTGTCAAGAGGCGTTTACCACTGATCAAGTCATATCAGCTGATTCTGTTAATACTTCTACCATGGTTTGGTGCAGCTGCCTTTTCTCAGGCAATGAGACCTTTCAACCATGTTTGTCATGTTGGAAAACAATGGGTTATACCAGTAGCTTCGCTGCATGTGTTGTACTTATTGGTGCTCATGGGTGTTACATGGGTCGTTAGCTTGGTTGTCTTTCAATTTGACGAATTCAAAGAGCTGAGCAAGGGAGTAACTATTACGCTTATATTCATAGGAGTTTGGGTTGCACTATACGTTGCTAATGAGGTTCATGATGAAAAATCAACAAGGCTTATGGTTGCATCGAGGTTCATGTTGATGCTTGCGGCCAATATTCTGGTTCTCTCCTTCTTTTTTATATCTATGTCACGACCTCTATTCACCCAACTGAGCATGAGGAAACGAGAATCTGATAAACTTGAGACAATGGGGCGCATATTAGGAGTTCCAGAAAGCGGATTGCTTTTTCCAACAGGCATGCCTTTGAATTTGGATGAACCTCTTGAAAAGCTGCTTTGTCAGAAAAAATTTCGACAATCTTTTATGGCTTTCGCAGATAGTCGAATGGCTGGGGAGAGTGTACACTTCTATGATGAAGTACATGAGTTGAATAAGATCCCGATTGGTGACACAGCACGTAGGATCTATATGGCAGGACATATAATTGAACAATATATAGCTGTTGATTCACCTATGGAGATTAACATATCACATCAGATGCGCCAAGAAATTTTGGACACAACTGATCTTGCTCATCCTGATCTCTTCACAAATGCAGTCAATGAGATGGTGCGAATGATGCAAATGAATCTGGAGAAGGACGTACCAGAATTCCACTTTCTATTCACGATTCAAGGAAGAGATCAGAGATGCAGCTGA___AGCATGTGAACTTTGGGACAATACAACTATTTGGGATTATTCTCCAAAAGTAAGTTTTGTCCATGGTACAGATGATCCCTTTGACCACGATAGCCTTTGCAGGAATTCTGAGGGATCATGGCAATCTTCCGTACAAAATAATAATGCCTGTCCATAAAACTCAAGTTCAATAAAGTCTTCAGTTCCATTTTTATTGCTAGATGAAGCTGATCTGCATCAATCATCGTTTAAGCAAATATAATGCAGAACCCTTTCAGTGGGAGCTAGGAAATTTCATTCATGGGATGGCTCCTTACCCTTGTCACTTCCATCTTGAAAATCCCATATGTTTAGTTTCTTTGATTTTGACATTTTGTCTATTCATCCAGGTTGGAATTACTATGACCTATTTCAAGAGATACATGATTGCACTTCAAGTTGGTTTTGTATCAGTTATCCAGAGCTGCAGTAGGCACGCTTGTAAATAAGAGATGTATTGCGGTGCCTATGTTTGATAATGTCTACCAGCAGTCTGAACTTTTCATCTAAGTTAATTTCGACCAAATTCGTACTGTTGTTTA

Fln Alignment:

Contig1125___SCYLWTVWLEGPCGFGLLMSCRRALAYRLYYLFVKRRLPLIKSYQLILLILLPWFGAAAFSQAMRPFNHVCHVGKQWVIPVASLHVLYLLVLMGVTWVVSLVVFQFDEFKELSKGVTITLIFIGVWVALYVANEVHDEKSTRLMVASRFMLMLAANILVLSFFFISMSRPLFTQLSMRKRESDKLETMGRILGVPESGLLFPTGMPLNLD--EPLEKLLCQKKFRQSFMAFADSRMAGESVHFYDEVHELNKIPIGDTARRIYMAGHIIEQYIAVDSPMEINISHQMRQEILDTTDLAHPDLFTNAVNEMVRMMQMNLEKD
D7TAJ5______________SCYLWAVWVEGPLGFGLLLSCRIVQAFQLYYIFVKRRLPPIRSYVFLPTIVLPWIAGAALLHKKKPLNERCHLGTRWIIPVVLLHTTYVAALVGFTVAIRHIEFRFHELKDLWRGILVSTSSVGLWVTAYILNEIHDDIEW-LQVTSRFLLLIMASILVLAFFSMSSSQPLLSKMSLRKREATEFETMGRALGIPDSGLLLQREPAPDIDPNEPLDKLLLNKGFRQSFMAFADSCLAGESVHFYDEVHELAKIPVDDPVRRIYMARHIIEKYVIAGATMEVNISHRNRQEILTTPDLAHPDLFNNALNELLQLMKMNLAKD

SNPs (tot: 59)

Position	A %	C %	G %	T %	Probability
767	90		10		0.90298
768	10			90	0.90298
769	90		10		0.90298
772		10		90	0.90298
773	10			90	0.90298
776		10		90	0.90298
780			10	90	0.90298
781			10	90	0.90298
782	90			10	0.90298
783		10		90	0.90298
785		10		90	0.90298
787	10			90	0.90298
789		90	10		0.90298
803	12	87			0.992554
805	87			12	0.992554
901	83		16		0.994512
903	83		16		0.994512
904	83			16	0.994512
906	80		20		0.995493
907		20		80	0.995493
908	20			80	0.995493
912		20	80		0.995493
915	80		20		0.995493
916			25	25	0.995533
918		25		75	0.996473
919	25			75	0.996473
920		25		75	0.996473
921			25	75	0.996473
922	25			75	0.996473
943		25	75		0.996473
1012		20	80		0.995493
1013		80	20		0.995493
1022	20	80			0.995493
1023	20			80	0.995493
1025	80			20	0.995493
1328		83		16	0.994512
1329	83			16	0.994512
1421	50		50		1.0
1446		85		14	0.993533
1453		14		85	0.993533
1466		20	80		0.995493
1496	80		20		0.995493
1501		60		20	0.99255
1505		20		80	0.995493
1509	80		20		0.995493
1510		20	80		0.995493
1511		80		20	0.995493
1512	20	20	20	40	0.994159
1514	60			40	1.0
1516			40	60	1.0
1517			60	40	1.0
1520	40	60			1.0
1521	20		40	40	1.0
1522	40	40	20		1.0
1524		20	80		0.995493
1525	33		66		0.997452
1529		66	33		0.997452
1533		33		66	0.997452
1534		66	33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain