SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene753

Length: 2242 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene753
ATGGGGACAACCCAACTCATTTGTAAGAATTTCGTCAGCAAGATCGGGGCAAAATTATTCGCATCCACGTCTAAGTTGTCACAAGCTTTGAATCTCGCTGCCCAAAATTCGCTTTCAAATTGGGATATTGGGAAATTTTTAATGGGAGGAGATGCAATCCCGAAAGTTATCGGCCCACGACTTTCGATCCGGGGCAGGACCGAAATTGCGGGCTTTCTGTTTAAACAAATGGAAAATCGAGGTTCCGCTCATAATAAATAATGGCGCCAGTGCGCCTGCTGTCACGATGTTTCACACACTGATTCTGTCGCAGCACTGTCTTTTTTGAGTACCTAGTTGCATGGCTAATTCCATGGCGGACACAGGCCGGGATCTGGAGATCAATTCCGACACTGCTTCGGTACCCGAACTCCAGAAACCACTTCTCTCGAACAGCATCCGAGACGCACCGGAATCCGACCAGGCCGATTTTGATCCCACGCTCGCGCGCCTGCAAACCGTTCTGGTGTTGCTGGGACTCGATCACTCGGCCGTGCTCAATACGTTGTTGGCTTGGCTTGCCTTCGTGGGGCTGGGAGCAGTGGTGCCTGCGGTCACCCTGTGGGCAACCAACTGCACGGACTGTCATAAAGTTCAGGTGCTTAAGTTTGAAGTGATGATACAGATCTCCGACTCCGGTTTAGCTGCAGTTTCTATGCTGTGCATGGCTTACAATATTAGGAAATATGGGCTCCGCAGGTTGCTTTTCCTGGACCAAACTGGCCATCCTCAAGTGCGTTTTCGCCTCGACTTCCCGAGTCAGCTCCAGGAAAGCTTTAAGCTAGTTAAATGGATTATCTTTCCATGCCTTGTGATGAAGATAACTCGAGAAGTTTGGCGGACTGTATATGTCCATCAAGGACCTTGGTGGGAAAATACGCTCATTCTTATAGCCATTATTATATCATGGCTTTATGTGACTGGCATTTTCCTCTTTGGCTGTGTTCTGTTCAACCTGGTATGCAGTATGCAAATTGTTCATGTTGAAGATTATGTGAGGCTTTTGGAAGGAACATCTGATGTTTCACTATTCTTAAAGGAGCACATGCAGCTTCGCTACCAATTGTATAAAATAAGTCATAGATTTCGTATATTTCTTCTTCTTGCTTTCTTGGTAGTGACTGGAAGCCAGTTTATGACCCTTTTTGAGACCACGTATTACAGTGGAACAATCAACTTTATCAATGCAGGTGATTTTCTGGTGTCCTCCATTGTCCAGGTGGTTGGTGTTATCCTTTGTTTGCGTGCAGCAGCAAAAAACTACCAATAGAGCACGAGGGATAGCCTCAGCTGCAAGTCAATGGCATGCTTGGGCAACATGCAATTCATCTGATGCGATTGAATCAAAGGATGAGGTTAATGGGGTTGGCACTGCAGAAGATGTATTTCCGATATATTCTCTATTTGCAGCTGAGTATGAGAGTGATATAGAGTCTGAAGCTTTAAATTTGCCTAATGATGCTCAGTTTGCTTCCCACGTGGCTTCTTACCACAAGAGACAAGCCCTTGTGATGTACTTTCAGTTCAACCCCACTGGAATCTCCATTTTTGGATGGCAAGTGGACCGTGGCTTGATGACTACATTATTATTTGTGGAGCTATCCCTGATAACTTGGGTTTTGGGAAAAACTATCATTGTAAGTTCAACAAAGTAAAAGACATTCCACTGTTATGGCTGATATCGAGATGGTAGCACACCACTGCGTGTGCTCTTTTCATGGTAATTGATAGTCTGATAGGAGAAACATCAGTGGCATTTTTGTTGCCGAGTACAGGATGTCATTGAGGAATCAATCACAGAGAAGAAAATTCGCAAGGAATACAGGTTGGCTTGGAAGTTGGGGTACTTGTGAATAATCAAGTTTGATTCAAGTGTAGTTTATGAAATAGTGTAATGTGTGATGTATTGTAAGACCAATTATGTTGTTATCTGCTATTTTTTTAGAGGATAAAGCGAGTAAGATCCATATCAATTTTGAGTGAAGTATTTTGGAGTCTTGAGGTTCCACTTGTTCAGAGAATGTGGAACCCTAATGGCTGTGGAAAAGAGGCCCCAGTGAATCCAGGTTAAATGTGATAATATCTTAATGTCTCATTTTACTTTTAACATTTTGTCAACGCTTTTCTCTTGTTCTCTGTCGGAGCCTGTATACTTAATTTTGAAAAAGAAACTGCACAACTGATCTCTGTAATATCAATCTTG

Ace file of the UniGene sp_v2.0_unigene753 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	DUF3537 multi-domain protein	-	-	37%
FL-Next	tr=Putative uncharacterized protein; Vitis vinifera (Grape).	-	-	51%
Blast2go	protein	-	-	64%

Source	GOs	Term	Type	e value	Identity
Blast2go	membrane	GO:0016020	Cellular Component	0.0	64%
Blast2go	nucleus	GO:0005634	Cellular Component	0.0	64%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: tr_plants

Fln subject: D7TAN3

Fln msg: Overlapping hits, possible frame ERROR between 1297 and 1296,

Fln protein: MANSMADTGRDLEINSDTASVPELQKPLLSNSIRDAPESDQADFDPTLARLQTVLVLLGLDHSAVLNTLLAWLAFVGLGAVVPAVTLWATNCTDCHKVQVLKFEVMIQISDSGLAAVSMLCMAYNIRKYGLRRLLFLDQTGHPQVRFRLDFPSQLQESFKLVKWIIFPCLVMKITREVWRTVYVHQGPWWENTLILIAIIISWLYVTGIFLFGCVLFNLVCSMQIVHVEDYVRLLEGTSDVSLFLKEHMQLRYQLYKISHRFRIFLLLAFLVVTGSQFMTLFETTYYSGTINFINAGDFLVSSIVQVVGVILCLRAAAxxTTNRARGIASAASQWHAWATCNSSDAIESKDEVNGVGTAEDVFPIYSLFAAEYESDIESEALNLPNDAQFASHVASYHKRQALVMYFQFNPTGISIFGWQVDRGLMTTLLFVELSLITWVLGKTIIVSSTK*

Protein Length: 453

Fln nts: ATGGGGACAACCCAACTCATTTGTAAGAATTTCGTCAGCAAGATCGGGGCAAAATTATTCGCATCCACGTCTAAGTTGTCACAAGCTTTGAATCTCGCTGCCCAAAATTCGCTTTCAAATTGGGATATTGGGAAATTTTTAATGGGAGGAGATGCAATCCCGAAAGTTATCGGCCCACGACTTTCGATCCGGGGCAGGACCGAAATTGCGGGCTTTCTGTTTAAACAAATGGAAAATCGAGGTTCCGCTCATAATAAATAATGGCGCCAGTGCGCCTGCTGTCACGATGTTTCACACACTGATTCTGTCGCAGCACTGTCTTTTTTGAGTACCTAGTTGC_-_ATGGCTAATTCCATGGCGGACACAGGCCGGGATCTGGAGATCAATTCCGACACTGCTTCGGTACCCGAACTCCAGAAACCACTTCTCTCGAACAGCATCCGAGACGCACCGGAATCCGACCAGGCCGATTTTGATCCCACGCTCGCGCGCCTGCAAACCGTTCTGGTGTTGCTGGGACTCGATCACTCGGCCGTGCTCAATACGTTGTTGGCTTGGCTTGCCTTCGTGGGGCTGGGAGCAGTGGTGCCTGCGGTCACCCTGTGGGCAACCAACTGCACGGACTGTCATAAAGTTCAGGTGCTTAAGTTTGAAGTGATGATACAGATCTCCGACTCCGGTTTAGCTGCAGTTTCTATGCTGTGCATGGCTTACAATATTAGGAAATATGGGCTCCGCAGGTTGCTTTTCCTGGACCAAACTGGCCATCCTCAAGTGCGTTTTCGCCTCGACTTCCCGAGTCAGCTCCAGGAAAGCTTTAAGCTAGTTAAATGGATTATCTTTCCATGCCTTGTGATGAAGATAACTCGAGAAGTTTGGCGGACTGTATATGTCCATCAAGGACCTTGGTGGGAAAATACGCTCATTCTTATAGCCATTATTATATCATGGCTTTATGTGACTGGCATTTTCCTCTTTGGCTGTGTTCTGTTCAACCTGGTATGCAGTATGCAAATTGTTCATGTTGAAGATTATGTGAGGCTTTTGGAAGGAACATCTGATGTTTCACTATTCTTAAAGGAGCACATGCAGCTTCGCTACCAATTGTATAAAATAAGTCATAGATTTCGTATATTTCTTCTTCTTGCTTTCTTGGTAGTGACTGGAAGCCAGTTTATGACCCTTTTTGAGACCACGTATTACAGTGGAACAATCAACTTTATCAATGCAGGTGATTTTCTGGTGTCCTCCATTGTCCAGGTGGTTGGTGTTATCCTTTGTTTGCGTGCAGCAGCAxxxxxxACTACCAATAGAGCACGAGGGATAGCCTCAGCTGCAAGTCAATGGCATGCTTGGGCAACATGCAATTCATCTGATGCGATTGAATCAAAGGATGAGGTTAATGGGGTTGGCACTGCAGAAGATGTATTTCCGATATATTCTCTATTTGCAGCTGAGTATGAGAGTGATATAGAGTCTGAAGCTTTAAATTTGCCTAATGATGCTCAGTTTGCTTCCCACGTGGCTTCTTACCACAAGAGACAAGCCCTTGTGATGTACTTTCAGTTCAACCCCACTGGAATCTCCATTTTTGGATGGCAAGTGGACCGTGGCTTGATGACTACATTATTATTTGTGGAGCTATCCCTGATAACTTGGGTTTTGGGAAAAACTATCATTGTAAGTTCAACAAAGTAA___AAGACATTCCACTGTTATGGCTGATATCGAGATGGTAGCACACCACTGCGTGTGCTCTTTTCATGGTAATTGATAGTCTGATAGGAGAAACATCAGTGGCATTTTTGTTGCCGAGTACAGGATGTCATTGAGGAATCAATCACAGAGAAGAAAATTCGCAAGGAATACAGGTTGGCTTGGAAGTTGGGGTACTTGTGAATAATCAAGTTTGATTCAAGTGTAGTTTATGAAATAGTGTAATGTGTGATGTATTGTAAGACCAATTATGTTGTTATCTGCTATTTTTTTAGAGGATAAAGCGAGTAAGATCCATATCAATTTTGAGTGAAGTATTTTGGAGTCTTGAGGTTCCACTTGTTCAGAGAATGTGGAACCCTAATGGCTGTGGAAAAGAGGCCCCAGTGAATCCAGGTTAAATGTGATAATATCTTAATGTCTCATTTTACTTTTAACATTTTGTCAACGCTTTTCTCTTGTTCTCTGTCGGAGCCTGTATACTTAATTTTGAAAAAGAAACTGCACAACTGATCTCTGTAATATCAATCTTG

Fln Alignment:

Contig753___DPTLARLQTVLVLLGLDHSAVLNTLLAWLAFVGLGAVVPAVTLWATNCTDCHKVQVLKFEVMIQISDSGLAAVSMLCMAYNIRKYGLRRLLFLDQTGHPQVRFRLDFPSQLQESFKLVKWIIFPCLVMKITREVWRTVYVHQGPWWENTLILIAIIISWLYVTGIFLFGCVLFNLVCSMQIVHVEDYVRLLEGTSDVSLFLKEHMQLRYQLYKISHRFRIFLLLAFLVVTGSQFMTLFETTYYSGTINFINAGDFLVSSIVQVVGVILCLRAAAxTTNRARGIASAASQWHAWATCNSSDAIESKDEVNGVGTAEDVFPIYSLFAAEYESDIES-EALNLPNDAQFASHVASYHKRQALVMYFQFNPTGISIFGWQVDRGLMTTLLFVELSLITWVLGKTIIVSS
D7TAN3_____________DETLQRLESFLCVLGFHQSSVLSIGLSWTVFFVIGVLLPVVVLELSNCEGCDKYQIKDFELDIVALQTCLAAVSLLCLSHNLRKYGIRKFLFVDRFSGQMVRFRDQYVQKITGSLRLLVSWILPCFLLKTAREVIRMLYVQHESWWLSVAILLALIVSWTYVSMISLTACTLFHLVCNLQVIHFDDYGKLLESESDVLVFMEEHIRLRHHLSKISHRFRIYLLLEFLVVTASQFVTLFQTTGYSGIITFINGGDFAVSSLVQVVGIILCLHAATxISHRAQGIASLASKWHAMSTCSSTDTSQLRTSTS-TGNLEAYNRLNSLHINYSESDLESLDYVVMPTSAQLASYMSSYHKRQAFVMYLQNNPGGITIFGWTVDRALINTIFFIELSLITFVLGKTIVFSS

SNPs (tot: 39)

Position	A %	C %	G %	T %	Probability
1281	83			16	0.994512
1414	83		16		0.994512
1466			87	12	0.992554
1528	25	75			1.0
1529		75	25		1.0
1531	75		25		1.0
1533		25		75	1.0
1535			25	75	1.0
1536		25		75	1.0
1537		25	75		1.0
1538	25			75	1.0
1540	75		25		1.0
1542		75		25	1.0
1545		25		75	1.0
1576	33		66		1.0
1828		42		57	1.0
1960	14	85			0.993533
1961	71	14		14	0.984337
1962		14	14	71	0.984337
1963		14	14		0.984337
1964	28	71			1.0
1965	14		85		0.993533
1974	83		16		0.994512
1975	33		66		1.0
1977		66	33		1.0
1978			66	33	1.0
1987		83		16	0.994512
1989	25	75			0.996473
1992			25	75	0.996473
1993		25		75	0.996473
1994	75	25			0.996473
1995	25			75	0.996473
1996	25			75	0.996473
1997			25	75	0.996473
1998	75		25		0.996473
1999		25		75	0.996473
2000		25		75	0.996473
2002		25		75	0.996473
2109	33	66			0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain