SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene748

Length: 1428 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene748 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Phospholipase C, putative n=1 Tax=Ricinus communis RepID=B9SCA3_RICCO	-	-	66%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	67%
Blast2go	pi-plc x domain-containing protein at5g67130-like	-	-	75%

Source	GOs	Term	Type	Identity
Blast2go	lipid metabolic process	GO:0006629	Biological Process	75%
Blast2go	phospholipase C activity	GO:0004629	Molecular Function	75%
Blast2go	anchored to plasma membrane	GO:0046658	Cellular Component	75%
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	75%
Blast2go	intracellular signal transduction	GO:0035556	Biological Process	75%

Source	InterPros	Term	Type	Identity
Sma3	Protease inhibitor I4, serpin	IPR000215	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Phospholipase C, phosphatidylinositol-specific , X domain	IPR000909	-	-
Sma3		IPR004141	-	-
Sma3	Epidermal growth factor-like	IPR006210	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Six-bladed beta-propeller, TolB-like	IPR011042	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	PLC-like phosphodiesterase, TIM beta/alpha-barrel domain	IPR017946	-	-
Sma3	Strictosidine synthase, conserved region	IPR018119	-	-
Sma3	Carbohydrate kinase, FGGY, conserved site	IPR018483	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5AAN6

Fln msg: Overlapping hits, possible frame ERROR between 116 and 64, your sequence is shorter than subject: 257 - 389

Fln protein: GTINPTEIVTIFIEDYVHATxxxxxxxxxxxxxxxxxxxMSKMPTNGEDWPTVTEMVANNQRLVVFTSMSSKEATEGIAYQWRYITENEPGDGGIKPGSCSNREESVALNSKAAILFLMNYFPTIPDQSGSCKDHSTALIQMLNVCYHVAGKAPNFIAVNFYMRSDGGGVFDAVDKVNGRSLCGCDTVAACQVGAANGVCRNVPVPSESSSTSGSSTSSQPLQASQSSHIFLFHFFSFQALYTYVMLSSLVAQAFF*

Protein Length: 258

Fln nts: ATGGGACCATAAATCCCACAGAAATTGTTACAATCTTCATTGAGGACTATGTACATGCCACCxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxATGTCTAAAATGCCTACGAATGGGGAAGATTGGCCAACTGTGACAGAGATGGTAGCCAACAATCAGCGTTTGGTAGTGTTCACTTCCATGTCCTCAAAGGAAGCAACAGAAGGCATTGCTTATCAGTGGAGATACATTACTGAAAATGAACCTGGTGATGGTGGCATCAAACCAGGCTCCTGCTCAAATCGCGAAGAATCAGTAGCCTTGAATTCAAAAGCAGCTATACTGTTTCTAATGAATTACTTTCCTACCATTCCTGATCAAAGTGGAAGCTGCAAGGATCATTCCACTGCACTTATCCAGATGCTCAATGTGTGTTATCATGTAGCAGGGAAAGCTCCCAATTTCATTGCAGTAAACTTCTACATGAGGAGTGATGGTGGAGGAGTATTCGATGCAGTAGACAAAGTCAATGGTAGATCCCTATGTGGATGCGATACTGTTGCAGCTTGCCAGGTAGGGGCAGCAAATGGAGTGTGCAGGAATGTGCCAGTTCCTTCTGAATCTTCTTCCACAAGTGGCTCCTCAACCTCTTCACAACCTCTGCAGGCGTCACAGTCTTCACATATCTTTCTCTTCCATTTCTTCAGTTTTCAGGCCTTGTACACTTATGTTATGTTATCATCCTTGGTGGCACAGGCCTTTTTCTGATGCATCTTGTTTTTGTTTTCTCCTCTTCCATAGCAGCATTGCATGAGTGAATAACCATTGATTCACACTTCCAGTTTTGTGTAGATGCTGTCGCCCTTTTCCAACCAAATAGAAAACGGCTAGCAGTTTGGAGGTTCAGATCTGACCCTGCCAGTTGGCCATTCTTTATCCAATAGATCCAGTCATAGATGACGATAAAGATGCTTGTGGCAGCTGCTGTCAAATTTGTTTCCAGGAAGACAATTATAAGCTTAACACCAAAATGTAGACTGTTCTTTTCATACCAAACTGTACATGATCCAGTAATTCTTGCAATGCCCTTGGTCCTAGGTACAAAACATTAGGACACAAGTCCAGAATATCTGGAAGCATATTCACAAAAGACTCTGTATTCTTTTCATGTTAAGCAAAGAAACGAGCAAAGTGATTGCATTTGCAGTCAAGAATCAATAGATGGGTGAATTTTAAATGTTTGGACTTCGACAGTTAAAAGGCAACCACTTGCATTCAAAACTATCCTTTCTAGACAACTGGGATATACTTCTTATTGGCAAAAGTTTACAGTGACTTTCAAAACTCTCTGTAGTCTCGATTATGTGAGTCAGTGTATGAGTACATAACCTTTTAACTTTAGATGTAATGTGGAAAGAAGACTAC

Fln Alignment:

Contig748___TINPTEIVTIFIEDYVHATxxxxxxxxxxxxxxxxxxMSKMPTNGEDWPTVTEMVANNQRLVVFTSMSSKEATEGIAYQWRYITENEPGDGGIKPGSCSNREESVALNSKAAILFLMNYFPTIPDQSGSCKDHSTALIQMLNVCYHVAGKAPNFIAVNFYMRSDGGGVFDAVDKVNGRSLCGCDTVAACQV
D5AAN6_____________SLNPTEIVTIFIEDYVHAIxxxxxxxxxxxxxxxxxxVSKMPMNGEDWPTVTEMVANNQRLVVFTSMPSKEATEGIAYQWRYITENEPGDGGIKPGSCSNRKESVPLNSKAAILFLMNYFPTIPDQSGTCKDHSTSLIQMLNVCYHGAGKAPNFIAVNFYMRSDGGGVFDAVDRVNGRSLCGCNTVAACQV

SNPs (tot: 5)

Position	C %	G %	T %	Probability
1144	12		87	0.992554
1269	16	83		0.994512
1270	16		83	0.994512
1273	83		16	0.994512
1329	16	83		0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain