SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene616

Length: 1869 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene616 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Serine carboxypeptidase S28 family protein [Arabidopsis thaliana] gb\|ABP88113.1\| At4g36190 [Arabidopsis thaliana] gb\|AEE86629.1\| Serine carboxypeptidase S28 family protein [Arabidopsis thaliana]	-	-	60%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	87%
Blast2go	probable serine protease eda2-like	-	-	75%

Source	GOs	Term	Type	Identity
Blast2go	proteolysis	GO:0006508	Biological Process	75%
Blast2go	serine-type peptidase activity	GO:0008236	Molecular Function	75%
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	75%

Source	InterPros	Term	Type	e value	Identity
Sma3	Peptidase S28	IPR008758	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NWA5

Fln msg: Separated hits, possible frame ERROR between 1264 and 1266,

Fln protein: MNVKWLLLAVIFITLSYLVRECSALPLPVGIKYRYSSSAYLTQDEYWFSQRLDHYSPTDHRQFNQRYYEFLDYFQAPDGPIFLKVCGEYPCDGITNDYMAVLAKKFGAAIVSLEHRYYGKSSPFKELTTHNLQYLSSKQALFDLAMFRNYYQELTNKKQNLSNYENSWFVFGVSYPGALSAWFRLKFPHLTCGSLASSAVVLAIYNFTDFDRQIGESAGPACKKALQEVTRLAEEGLSTSANAVKSLFGAEKLNNDGDFLYLLADAAVMAFQYGNPDVLCSPLVKANSSGQDLMVAYADYVKNYISDESVESYGQQFLKKTTIEADSNDGQCYRCSDLCTEFAYFQVxxNGSIRSSKVDTLYHLVLCENVFGKDIYPEVDITNVYYGGTSIAGSKIIFMNGSQDPWRHASKQKSSDNMPSVLITCHNCGHGVDLRGCPQSPSRIEGDASDCASPDVVHKARQQMVEHIDLWLSQCQDQDHGGIFLV*

Protein Length: 488

Fln nts: CGTCGTGACCTTGGCCGTCGTGACCTTGGCTGTCACTCAGGTATTACAATCAATTCAATCCTGCTCTCATTTCCAAAGGGTTCGTTCGATTCCCGTCCGAAGAAACAGGCTTGGAAGCAAGTGAAATGCATAATAGTGGTTGAACGCTTTCAAGTCGTTGATTCATCTAGTAGAAGATGTAGAGAGATTGACAATATAATTGACATCTGGCATCAGCAGA_-_ATGAATGTGAAGTGGCTTTTGCTTGCAGTCATCTTTATCACTTTGTCCTATCTGGTTCGGGAGTGTTCAGCTCTGCCATTGCCAGTGGGGATCAAATACCGCTATAGCTCAAGCGCCTACTTGACCCAAGATGAATACTGGTTTTCTCAGAGACTGGACCACTATTCTCCCACGGACCATCGCCAGTTTAACCAAAGGTATTATGAGTTCCTTGATTACTTTCAGGCACCTGATGGACCAATATTTTTAAAAGTCTGTGGTGAATATCCTTGTGATGGCATTACGAATGACTATATGGCAGTTTTGGCAAAGAAGTTCGGTGCTGCTATTGTTTCCCTTGAGCATAGATACTATGGAAAGAGCTCTCCATTCAAAGAATTGACGACACATAATCTACAATATCTTTCTTCTAAGCAAGCACTTTTTGACTTGGCTATGTTTCGTAACTATTACCAGGAATTGACAAACAAAAAGCAAAATCTGTCCAACTACGAGAACTCTTGGTTTGTTTTTGGCGTGTCATACCCAGGGGCCCTTAGTGCATGGTTCCGCCTGAAGTTTCCTCACCTTACTTGTGGAAGCCTTGCAAGCTCAGCTGTTGTTCTAGCTATTTACAACTTCACAGATTTTGACAGACAGATCGGGGAGTCAGCAGGGCCTGCCTGCAAAAAGGCTCTGCAAGAAGTCACAAGGCTTGCTGAGGAGGGTTTATCTACTAGTGCAAATGCTGTAAAGTCTTTGTTTGGTGCAGAAAAGTTGAATAACGATGGCGACTTTCTTTACCTATTGGCAGATGCTGCAGTGATGGCGTTTCAATATGGGAATCCCGATGTACTTTGCTCTCCTCTTGTTAAAGCAAATTCATCAGGACAAGATTTGATGGTTGCTTATGCAGATTATGTAAAAAATTATATATCTGATGAAAGTGTTGAAAGTTACGGCCAACAGTTCCTGAAAAAAACAACTATTGAAGCTGATAGCAACGATGgtcagtGcTaTCGGTgtTcagAtcTTTGCACAGAATTTGCCTACTTTCAAGTTTCxxxCaATGgCAGTATACGATCGTCGAAGGTGGATACtcTGTACCaTCTGgtTCTCTGTGAAAATGTTTTTGGCAAAGACATATATCCTGAAGTGGATATAACCAATGTGTACTATGGAGGCACTAGCATTGCAGGTTCAAAGATAATTTTTATGAATGGTTCACAAGATCCATGGAGACATGCATCCAAGCAAAAGTCATCTGATAATATGCCGTCGGTTCTGATCACATGTCATAATTGTGGTCATGGTGTTGACCTTCGAGGATGTCCTCAGTCACCTTCCAGAATCGAGGGGGACGCTTCTGATTGTGCTTCCCCAGACGTAGTGCACAAGGCAAGGCAACAAATGGTTGAGCATATTGACCTATGGCTTTCACAATGCCAAGATCAAGATCACGGTGGGATCTTTTTAGTGTAG___AAAGTTGATTTTGATTGAATAATTTGTCTGAATGTGTAAATGAAGAATAATTATATACATTAGTTTTTAGTAGAAATAAAATGCATTCATGCTTACAGTACAAAATATTGACGTTTAATTAATGGTGTTGATGTATTTTGATTGAATAATTTGTTTGAATTTGTAAATAAAAAAATAATTATGTGCATTA

Fln Alignment:

Contig616___MNVKWLLLAVIFITLSYLVRECSALPLPVGIKYRYSSSAYLTQDEYWFSQRLDHYSPTDHRQFNQRYYEFLDYFQAPDGPIFLKVCGEYPCDGITNDYMAVLAKKFGAAIVSLEHRYYGKSSPFKELTTHNLQYLSSKQALFDLAMFRNYYQELTNKKQNLSNYENSWFVFGVSYPGALSAWFRLKFPHLTCGSLASSAVVLAIYNFTDFDRQIGESAGPACKKALQEVTRLAEEGLSTSANAVKSLFGAEKLNNDGDFLYLLADAAVMAFQYGNPDVLCSPLVKANSSGQDLMVAYADYVKNYISDESVESYGQQFLKKTTIEADSNDGQCYRC--SDLCTEFAYFQVSxNGSIRSSKVDTLYHLVLCENVFGKDIYPEVDITNVYYGGTSIAGSKIIFMNGSQDPWRHASKQKSSDNMPSVLITCHNCGHGVDLRGCPQSPSRIEGDASDCASPDVVHKARQQMVEHIDLWLSQCQDQDHGGIFLV
A9NWA5_____________MNVKWVLLAVILVIFSYVVLECSALPLPVGIKSGRSSSAYLTQDEYWFSQRLDHYSPTDHRQFNQRYYEFLDYFQAHDGPIFLKVCGEYSCDGIANDYMAVLAKKFGAAIVSLEHRYYGKSSPFKESTTHNLQYLSSKQALFDLASFRNYYQELTNKKQNLSNYDNSWFVFGVSYPGALSAWFRLKFPHLTCGSLASSAVVLAVYNFTDFDRQIGESAGPGCKKALQEVTRLAEQGLSTNANAVKSLFGAEKLKNDGDFLYLLADAAVTAFQYGYPDVLCSPLVEASSSGKDLMVAYADYVKNYSSDGGVESYGQQFLKNTTVDADSNGVSNSRSWWYQVCTEFAYFQVAxNDSVRSSKVDTKYHLDLCENVFGKGIYPEVDITNLYYGGTSIAGSKIIFMNGSQDPWRHASKQKSSDNMPSYIITCHNCGHGTDLRGCPQSPSRIEGDASDCASPDVVHKARQQMVEHIDLWLSQCQDEDHGGIFVV

SNPs (tot: 19)

Position	A %	C %	G %	T %	Probability
998		87		12	0.992554
1149	90	10			0.90298
1151		90		10	0.90298
1152		90		10	0.90298
1607		10	90		0.999898
1835	11	44			1.0
1837		20		80	0.999996
1838		22	66	11	0.999999
1839	77	22			0.999999
1840	22		66	11	0.999999
1843	85			14	0.993533
1844	14	85			0.993533
1846			85	14	0.993533
1847	14	85			0.993533
1848		85	14		0.993533
1852			85	14	0.993533
1854		85	14		0.993533
1856		85	14		0.993533
1860	14		85		0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain