SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene590

Length: 3259 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene590
GAAAAGAATAACTATATTACAAACAAGAATTACATGCATATGAAAATATGCTTGTCCTGGAATATCATTACTAGATTTAACAAAAAGAAAATATTCGACTAGATTCTCAAGATCTCCCTGAAACATCAAAGAGGAATAGCCGGTACACTTTGGATAATAAAAAGGCCACTTTGCCTTCTTAACTGCCAATAGTCCAGTTCATGCAAGTAACAAGCATTTTGAAGTAAACATTGCAACAGCACCGTAGTTCCAAGTGGTGTTCCCTCCAAAAATTTATGTGCCGAAATCATGGACCATTATGCCCATTATTCCACCCTTGAAACTTGAAGCAGCTCATATGAATAAGTATACATAATATCAACCAGCAGTTTAGTCCAGAAAGATTGTGAGATTTACTTTCTCCTTGTCTTGTCCCGCTTTGCCTTTATAAACTTTCTATTACCCCTATCACCTCCAGCACCAAGAGAGAAGCCTCCACCAGAAAACTCAAGCCCACTGCCACCTGCAGGATTGAACGTGCTTGGCGCACTGGATGCAAAAGGATTAGCTGCTGCTGGACCAGGCTGGGCACCAAACTGGAAAACAGATTGCCCAGCAGACGGAGCAGTTGGCTGAGAGCCAAACACGAAAGATGGAGCTGGTGAAGCAGTCTGTCCACCAAATACTGGTGGAACAGGACTTGATACCTGGGCAGGATCCTCTGCCATGCTGTCTTCCATCTGATCAGCCGGTGGTGTTGGTGATGGTGCAGCCCCAAAAGCAGAACTTGAGTTGGTCAACCCAAAAATTGGTTGAGTCATAGAAGAACTGTCTTTCGAAGCAAATGTAAATGTGCTAGGGGGGTGGTACCAGTTGATCCAAACGAGAATGTAGTTCCAGATGGAGTGCTTCCTGCACCTCCAAAAGTAAAGGAGCCACTGCTTGCAGCTGCAGATCCACCAAAAGAAAAACCTGTAGATGGCAAGGATGCAGTGCCCCCAAACCCAGATCCAAACGAGGTCATTGTTGACTGTGAAGATGTTGTACTGGAAGCTCCAAAAGGCTGTCCCAAGCCAAATGATGGAGTGCTGTTTGAACCAAATAATGGAGTGCTAGCAGAACCATACGAGGCACTGCCTCCACCAAATGCTGGAGTGCTGGCAGCACCAAATGACACACTGCTTCCACCAAATGCTGGAGTGCTGGCAGAAACAAAAGAGGAGCTGCTTCCACCAAATGAAGGAGAACTGCTAGATCCAAATGTACTCCCAGAACCAAATGAGGGGGTGCTGCCAGAACCAAATGGAGGAGTGCTCCCAGAACCAAACGCAGGAGTGCTGCCAGAACCAAATGAAGGGGTGCTCCCTGTGGTAAAGGAAGATGTATTTGAACCAAATGTTACACCAGGGACAAACGAAGGTGCAGTGCTGGAACTAGATTGAAGACTGGTTAATCCAAATGAGGGAGATGTACTGAGAACCAAGGTGACCGGCGTGGTGCTCGACGCAAACGAAGGAGAAGTGGTGGAACCAAACTGTGGAGTAGTACTTAACCCAAATGAAGGAGAGGTATTTGAACCAAACTGAGAGGTGCTACCCAATCCAAATGAAGTACTGCTGCTACCAGAGAAATTAGAAGTGCCGGCGGAACCTACTGAGAGGCTACTGCCAGCACCAAATGTAGGTGCCGTGCCCGACCCAGGTGCAGGGGTACTACTAGAAGCAAATTGGGCAGAAGTGCTACTATTGCTGAGGCCAAAAGAAGGTAGACTGGATGAGCTTAATGAACTGGGTGCAGTTAAGCCAAATAAGCTACCTGAAGCACCCAAAGCCGAAGCAGACAGAGTTGACCCGAACAAAGAGGGGCTTGTATTCCCACTAGAAAGAGACGATGAGTTGGCAGAACTCCCAAACACAAATGAACCTGAGGTAGTAGCTGTAGCAGAACTTGGCACCTGCCCAAAGAGAGATCCTGCAAAAGAAGACTGCGTATTGGGAAGGAGACTACTTGTAGGAGCTGAAATTGCAGTTGTGCTGGATAGAGTGCTTAAAGTACCATTGGTTACTCCAAGAGATGGGGAGGAAGCTAAACCACTGCTTTTAGGTTCATTTTGGGATTTTATTGTGCAATTTTCACCACTGGTGGAGCTTTTAATTGATTGATCTTCTGCTACTACAGATGATGCTTCAACCTGTGTAAGAGTGTCAGGACTTCTCAATTTGGATTCCACCTTTGCGGTAGTGGCTGCCAGAGCTGCTCTTTCCAGCAAACGAAAATGGAACTGAGTTCATACCATTTACCTTGGAGACAGATCCACCGAACAATGGGAGGCCTGCAGAAGTTGTGGCGACAGTGGTAGATGTAGAGAACATACTGCTTGAACTTGAGAAAGCGAGGGAACTCTCTGATAATCCAGAGGTTGATGAACCCTTAAAGGTTGCAGCTAAAGGTTCTGATCCATCTGTAGGTGCAGATGCAGCTGCAGCAACAGCAGTACTGAAAGATGATTCTGGCTTGCTTTCAAATTTGAAATCAAACTTCGGCTTGGTAGTATCAACATCTGTGGATCCACTTGCAGAAAAAGAGAAGACGGGCCCTTTGCTGGGACTTGCTGAACCAAAGCTGTAAGTTGGAGTAACATTGGCTGTAGTAGGCTGAGTGTTACCTTTATTTAAAGGAGATGGAATGACTGAGGGAGTGGGTGGTGGTTCTGCAAAATTACTATTAGATGGAGTAACAGGAAAAGTAAACCGTGACCTGTTATTTGTCGATGAATCAAGGGAAACAGAAGTTGTGGATGTAACTAAATTTTCTTCAGATCTTTTTCGCAATACAGTAGTAGATGTGATTCGTTTATCTCCATCCAATATTATACTATTAGCACCATTGCCATCAGATGTAAAAGATGGGACCTTTCCAGTCTGGGCATGACGTGACATGTCCAGCATTCCTGCTTGCATGGCCCTCATCATCTGACTCCTCAAGAGCACTCATTCGAAAGCCTTTACTCTTTTCTGGAAGATTAGAAGGGCCATCTAAAATCAGTAATTTTGCATCAATTCTTGCAGGACTTGCATCAGGAATTAGGGAAGATACATCTCCAATACCAGAAGATGTAGCTGCCTCCACAGACGTTGCCACTGAATCGAACAGAGGAGATTTACCTTTTCCCCCATATTTCTGTTCAGCACTAGTGACAGCCAAGGTCACGACGAGCTGGTTATTTTTCTGAACAGAAGTTGGACTATACTTATCAAAAGGGGAGTTGCATCTATCCCAT

Ace file of the UniGene sp_v2.0_unigene590 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Herpes_gp2 multi-domain protein	-	-	2.0e-24	23%
FL-Next	tr=Putative uncharacterized protein; Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress).	-	-	0.0	36%
Blast2go	protein	-	-	1.23037e-17	49%

Source	InterPros	Term	Type	e value	Identity
Sma3	Peptidase S59, nucleoporin	IPR007230	-	0.0	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: tr_plants

Fln subject: D7KXT7

Fln msg: query STOP codon is far from subject stop. Distance to subject end: 27 aas, W1: There is no M at the beginning,

Fln protein: VGQPKNWLSHRRTVFRSKCKCARGVVPVDPNENVVPDGVLPAPPKVKEPLLAAADPPKEKPVDGKDAVPPNPDPNEVIVDCEDVVLEAPKGCPKPNDGVLFEPNNGVLAEPYEALPPPNAGVLAAPNDTLLPPNAGVLAETKEELLPPNEGELLDPNVLPEPNEGVLPEPNGGVLPEPNAGVLPEPNEGVLPVVKEDVFEPNVTPGTNEGAVLELD*

Protein Length: 218

Fln nts: GAAAAGAATAACTATATTACAAACAAGAATTACATGCATATGAAAATATGCTTGTCCTGGAATATCATTACTAGATTTAACAAAAAGAAAATATTCGACTAGATTCTCAAGATCTCCCTGAAACATCAAAGAGGAATAGCCGGTACACTTTGGATAATAAAAAGGCCACTTTGCCTTCTTAACTGCCAATAGTCCAGTTCATGCAAGTAACAAGCATTTTGAAGTAAACATTGCAACAGCACCGTAGTTCCAAGTGGTGTTCCCTCCAAAAATTTATGTGCCGAAATCATGGACCATTATGCCCATTATTCCACCCTTGAAACTTGAAGCAGCTCATATGAATAAGTATACATAATATCAACCAGCAGTTTAGTCCAGAAAGATTGTGAGATTTACTTTCTCCTTGTCTTGTCCCGCTTTGCCTTTATAAACTTTCTATTACCCCTATCACCTCCAGCACCAAGAGAGAAGCCTCCACCAGAAAACTCAAGCCCACTGCCACCTGCAGGATTGAACGTGCTTGGCGCACTGGATGCAAAAGGATTAGCTGCTGCTGGACCAGGCTGGGCACCAAACTGGAAAACAGATTGCCCAGCAGACGGAGCAGTTGGCTGAGAGCCAAACACGAAAGATGGAGCTGGTGAAGCAGTCTGTCCACCAAATACTGGTGGAACAGGACTTGATACCTGGGCAGGATCCTCTGCCATGCTGTCTTCCATCTGATCAGCCGGTGGTGTTGGTGATGGTGCAGCCCCAAAAGCAGAACTTGAGTTGGTCAACCCAAAAATTGGTTGAGTCATAGAAGAACTGTCTTTCGAAGCAAATGTAAATGTGCTAGGGGGGTGGTACCAGTTGATCCAAACGAGAATGTAGTTCCAGATGGAGTGCTTCCTGCACCTCCAAAAGTAAAGGAGCCACTGCTTGCAGCTGCAGATCCACCAAAAGAAAAACCTGTAGATGGCAAGGATGCAGTGCCCCCAAACCCAGATCCAAACGAGGTCATTGTTGACTGTGAAGATGTTGTACTGGAAGCTCCAAAAGGCTGTCCCAAGCCAAATGATGGAGTGCTGTTTGAACCAAATAATGGAGTGCTAGCAGAACCATACGAGGCACTGCCTCCACCAAATGCTGGAGTGCTGGCAGCACCAAATGACACACTGCTTCCACCAAATGCTGGAGTGCTGGCAGAAACAAAAGAGGAGCTGCTTCCACCAAATGAAGGAGAACTGCTAGATCCAAATGTACTCCCAGAACCAAATGAGGGGGTGCTGCCAGAACCAAATGGAGGAGTGCTCCCAGAACCAAACGCAGGAGTGCTGCCAGAACCAAATGAAGGGGTGCTCCCTGTGGTAAAGGAAGATGTATTTGAACCAAATGTTACACCAGGGACAAACGAAGGTGCAGTGCTGGAACTAGATTGA___AGACTGGTTAATCCAAATGAGGGAGATGTACTGAGAACCAAGGTGACCGGCGTGGTGCTCGACGCAAACGAAGGAGAAGTGGTGGAACCAAACTGTGGAGTAGTACTTAACCCAAATGAAGGAGAGGTATTTGAACCAAACTGAGAGGTGCTACCCAATCCAAATGAAGTACTGCTGCTACCAGAGAAATTAGAAGTGCCGGCGGAACCTACTGAGAGGCTACTGCCAGCACCAAATGTAGGTGCCGTGCCCGACCCAGGTGCAGGGGTACTACTAGAAGCAAATTGGGCAGAAGTGCTACTATTGCTGAGGCCAAAAGAAGGTAGACTGGATGAGCTTAATGAACTGGGTGCAGTTAAGCCAAATAAGCTACCTGAAGCACCCAAAGCCGAAGCAGACAGAGTTGACCCGAACAAAGAGGGGCTTGTATTCCCACTAGAAAGAGACGATGAGTTGGCAGAACTCCCAAACACAAATGAACCTGAGGTAGTAGCTGTAGCAGAACTTGGCACCTGCCCAAAGAGAGATCCTGCAAAAGAAGACTGCGTATTGGGAAGGAGACTACTTGTAGGAGCTGAAATTGCAGTTGTGCTGGATAGAGTGCTTAAAGTACCATTGGTTACTCCAAGAGATGGGGAGGAAGCTAAACCACTGCTTTTAGGTTCATTTTGGGATTTTATTGTGCAATTTTCACCACTGGTGGAGCTTTTAATTGATTGATCTTCTGCTACTACAGATGATGCTTCAACCTGTGTAAGAGTGTCAGGACTTCTCAATTTGGATTCCACCTTTGCGGTAGTGGCTGCCAGAGCTGCTCTTTCCAGCAAACGAAAATGGAACTGAGTTCATACCATTTACCTTGGAGACAGATCCACCGAACAATGGGAGGCCTGCAGAAGTTGTGGCGACAGTGGTAGATGTAGAGAACATACTGCTTGAACTTGAGAAAGCGAGGGAACTCTCTGATAATCCAGAGGTTGATGAACCCTTAAAGGTTGCAGCTAAAGGTTCTGATCCATCTGTAGGTGCAGATGCAGCTGCAGCAACAGCAGTACTGAAAGATGATTCTGGCTTGCTTTCAAATTTGAAATCAAACTTCGGCTTGGTAGTATCAACATCTGTGGATCCACTTGCAGAAAAAGAGAAGACGGGCCCTTTGCTGGGACTTGCTGAACCAAAGCTGTAAGTTGGAGTAACATTGGCTGTAGTAGGCTGAGTGTTACCTTTATTTAAAGGAGATGGAATGACTGAGGGAGTGGGTGGTGGTTCTGCAAAATTACTATTAGATGGAGTAACAGGAAAAGTAAACCGTGACCTGTTATTTGTCGATGAATCAAGGGAAACAGAAGTTGTGGATGTAACTAAATTTTCTTCAGATCTTTTTCGCAATACAGTAGTAGATGTGATTCGTTTATCTCCATCCAATATTATACTATTAGCACCATTGCCATCAGATGTAAAAGATGGGACCTTTCCAGTCTGGGCATGACGTGACATGTCCAGCATTCCTGCTTGCATGGCCCTCATCATCTGACTCCTCAAGAGCACTCATTCGAAAGCCTTTACTCTTTTCTGGAAGATTAGAAGGGCCATCTAAAATCAGTAATTTTGCATCAATTCTTGCAGGACTTGCATCAGGAATTAGGGAAGATACATCTCCAATACCAGAAGATGTAGCTGCCTCCACAGACGTTGCCACTGAATCGAACAGAGGAGATTTACCTTTTCCCCCATATTTCTGTTCAGCACTAGTGACAGCCAAGGTCACGACGAGCTGGTTATTTTTCTGAACAGAAGTTGGACTATACTTATCAAAAGGGGAGTTGCATCTATCCCAT

Fln Alignment:

Contig590___EPLLAAADPPKEKPVDGKDAVPPNPDPNEVIVDC---------EDVVLEAPKGCPKPNDGVLFEPNNGVLAEPY----------EALPP---------PNAGVLAAPNDTLLP-PNAGVLAETKXXXXXXXXXXXXXPNVLPEPNEGVLPEPNGGVLPEPNAGVLPEPNEGVLPVVKEDVFE-PN
D7KXT7_____________EPVIGLARKPWAPKVD---CVEPKELLPNAEVDCPKAGEEPKLKDGVLDAPKA----GAGVLDAPNAGVLDAPKAPPELKALLPNALPELPKAGAGDTPNAGELEEPNEGVVAAPKAGVLLEPKA-------------DVLDAPNPGVLVAPNPGVLVAPNPGVLVAPNAEVFEAPNAEVFEAPN

SNPs (tot: 113)

Position	A %	C %	G %	T %	Probability
510	40		60		1.0
519	40	60			1.0
520	40			60	1.0
522	40		60		1.0
523	40		60		1.0
525	40			60	1.0
533		60	40		1.0
536		40		60	1.0
537	40			60	1.0
539	16	83			0.994512
540	16			83	0.994512
542			16	83	0.994512
549		16		83	0.994512
611		16		83	0.994512
646		16	83		0.994512
657			16	83	0.994512
886	85		14		0.993533
891	14	85			0.993533
893		14		85	0.993533
911			90	10	0.90298
912		90	10		0.90298
913	90		10		0.90298
940	18		81		0.999994
944		72		9	0.999994
966	18		81		0.999994
970	36		63		1.0
1005	63		36		1.0
1020			10	90	0.90298
1026	88			11	0.923564
1027	88	11			0.923564
1029		11	88		0.923564
1030	88			11	0.923564
1031		11	88		0.923564
1032	11	88			0.923564
1087		88	11		0.923564
1123		18	81		0.999994
1124	81		18		0.999994
1128	81		18		0.999994
1130		81	18		0.999994
1132	81	18			0.999994
1135	80		20		0.999996
1136	80		20		0.999996
1137	80	20			0.999996
1138	80			20	0.999996
1141	10			90	0.90298
1145	88			11	0.923564
1147	11	88			0.923564
1281	80	20			0.995493
1284	80	20			0.995493
1287		80	20		0.995493
1289	20			80	0.995493
1294		80	20		0.995493
1295		80	20		0.995493
1297	80			20	0.995493
1298	80		20		0.995493
1302			80	20	0.995493
1540	33			66	1.0
1541			33	66	1.0
1543	33		66		1.0
1544	33	66			1.0
1855	75	8		16	0.99999
1858	8		16	75	0.99999
1860		83		16	0.999991
1861	75		16	8	0.99999
1862			75	25	1.0
1867		16	83		0.999991
2007	8	92			0.999718
2028	89			3	0.998606
2029		7		92	0.999532
2034	92	7			0.999532
2036		92	7		0.999532
2039			92	7	0.999532
2056		11	88		1.0
2057			94	5	0.998894
2061	91	5		2	0.995912
2062	85	8		5	0.999999
2063		5	94		0.998894
2064		88	2	8	1.0
2065	94			5	0.998894
2066		2	91		0.995912
2069		93	6		0.99903
2073	6			93	0.999264
2074		6		93	0.999264
2411		50	50		1.0
2765		88	11		0.923564
2767		11	88		0.923564
2769			11	88	0.923564
2770	11		77		0.968132
2776			87	12	0.992554
2885	80	8			1.0
2886	72		16		1.0
2890	8			92	0.999718
2891		8		92	0.999718
2894			92	8	0.999718
2896	88		8		0.999262
2899	92			8	0.999718
2900	92	8			0.999718
2907	8		92		0.999718
2925			10	89	1.0
2927	89	10			1.0
2928			10	89	1.0
2930		89	10		1.0
2932		10		89	1.0
2933	89	10			1.0
2935			89	10	1.0
2936	89		10		1.0
2942		11		88	1.0
3039	93			6	0.999999
3101	37		62		1.0
3122			88	11	1.0
3218	80			20	0.995493
3219	80	20			0.995493
3244		75		25	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain