SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene572

Length: 1817 nt

Origin of reads: Inia v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene572 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	AMP dependent ligase, putative n=1 Tax=Ricinus communis RepID=B9S6X4_RICCO	-	-	55%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	51%
Blast2go	amp-binding protein	-	-	72%

Source	GOs	Term	Type	e value	Identity
Blast2go	ligase activity	GO:0016874	Molecular Function	0.0	72%
Blast2go	peroxisome	GO:0005777	Cellular Component	0.0	72%

Source	InterPros	Term	Type	Identity
Sma3	AMP-dependent synthetase/ligase	IPR000873	-	-
Sma3	Lipocalin	IPR002345	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Glycoside hydrolase, family 5, conserved site	IPR018087	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NWM6

Fln msg: atg_distance in limit (1-15): atg_distance = 12, W2: There is no M at the beginning,

Fln protein: LKRCDANDSPLSPIGFMERAATVYGEMTSVIYNDSRFTWCQTFDRCRRLASALSSRNISRGDVVSVVAPNIPALYELHFAVPMAGAVLNNVNIRLNAPAMAIQFRHCQPKFVFVDYQYLPLVLEAIKGTDHKPCLVVIEEESDTDRGKEYLDTTTKITTYHGLLREGDPVFEPRQPKDEWEPIVLNYTSGTTSAPKGVVNSHRAAYISTMNILSMWGIKPRPVFLWTLPMFHNNGWSFPWGIAATGGTNICLRKFDAKLIYSYIQDHGVTHFCAAPVVLSMIANASSSDRKPLPGRVEILTSGASPPSSDLLRMEELGFSVSHGYGLTESGGFVTLCAWKPEWDKLTAVDRSKLRVRQGVGVLTLVQVDVKDPASMVTVPRDGKTMGEIMLRGSTVMMGYLNDERSTAKAMEGGWFHTGDLGVIHSDGYLEIKDRSKDIIISGGENISSVEVESVLYCHPMIAEAAVVGRPDPFWGETPCAFVTNRVKASDPKLSGVRVSEAEMISFCRERLPHYMVPKTVVFMESLPKTSTGKIQKFLLREMAKLSSISSSLRGVGGMYSATA*

Protein Length: 566

Fln nts: CTCAAGCGCTGTGATGCGAATGACAGTCCCTTGTCTCCAATAGGATTCATGGAGAGAGCCGCGACTGTCTATGGAGAAATGACATCAGTCATCTACAATGACAGTCGCTTCACATGGTGTCAAACATTCGACCGCTGTCGCAGACTTGCATCTGCTCTGTCTTCTCGCAATATTTCCCGAGGGGATGTGGTGTCCGTTGTCGCTCCCAACATTCCTGCACTATATGAACTGCATTTCGCAGTACCTATGGCCGGAGCAGTTCTCAACAACGTCAACATCCGTCTCAATGCTCCAGCAATGGCCATTCAATTCCGCCATTGCCAGCCCAAATTTGTATTCGTCGATTACCAATATCTGCCTCTGGTCCTGGAAGCCATTAAGGGAACTGACCATAAACCATGCCTGGTCGTGATTGAAGAAGAGAGCGACACCGATCGTGGCAAAGAATATTTGGACACCACTACAAAAATTACTACATACCATGGACTGCTGCGGGAAGGAGATCCAGTGTTCGAACCCAGGCAACCCAAGGACGAATGGGAACCGATCGTTCTGAACTACACATCGGGTACCACTTCGGCTCCCAAAGGAGTGGTGAACAGCCACAGGGCTGCATACATCAGCACAATGAATATTCTCTCAATGTGGGGAATCAAGCCACGGCCTGTCTTTCTGTGGACTCTGCCCATGTTCCACAATAATGGCTGGAGTTTTCCATGGGGAATAGCAGCTACTGGGGGGACAAATATATGCCTAAGAAAATTCGATGCTAAGCTCATATATTCATATATACAGGATCATGGAGTTACCCATTTCTGTGCCGCCCCGGTCGTGCTCTCCATGATCGCCAATGCGAGTTCGTCAGATCGGAAACCCTTGCCGGGCAGGGTTGAAATCTTGACCTCGGGAGCTTCCCCGCCATCTTCTGATCTGCTGAGAATGGAAGAGCTGGGCTTCTCTGTTAGTCATGGCTATGGGCTCACAGAATCTGGAGGGTTCGTCACACTCTGCGCCTGGAAGCCTGAATGGGATAAATTGACAGCTGTGGACAGATCCAAACTCAGGGTCAGGCAAGGCGTTGGGGTTCTGACTTTGGTTCAAGTCGATGTAAAGGATCCAGCGTCGATGGTTACCGTGCCCAGAGATGGCAAAACGATGGGCGAAATTATGCTGAGAGGAAGCACTGTGATGATGGGATATCTCAATGACGAGAGAAGCACAGCCAAAGCCATGGAAGGGGGCTGGTTCCACACGGGGGATCTTGGAGTGATTCACTCGGACGGTTATCTGGAAATAAAGGATCGTTCCAAAGACATTATCATCTCAGGGGGAGAAAACATTAGCAGTGTGGAGGTTGAGTCTGTGTTGTATTGCCATCCCATGATTGCTGAGGCGGCTGTGGTTGGGAGGCCTGATCCTTTCTGGGGGGAGACCCCATGTGCCTTTGTGACTAACAGAGTTAAAGCGAGCGATCCTAAGCTTTCTGGTGTAAGAGTATCAGAGGCTGAAATGATTTCGTTTTGCAGAGAGAGGCTGCCTCACTACATGGTTCCCAAAACCGTCGTCTTCATGGAGTCACTGCCCAAGACGTCCACTGGTAAAATTCAAAAGTTTCTTCTCAGGGAAATGGCGAAACTTTCTTCGATCAGTTCTTCCCTACGTGGCGTTGGTGGAATGTATTCTGCGACGGCATAA___CATTCGTTTGTATGCTGATTGTGCTTATCTCATTGTATGCTAATTGTGCGGGTGGGTCGATGTTTGTGCGCTGGTTTTAGGCGGCGCTTAAATTCTACGCGTATATATTTATTTTTTCCTTT

Fln Alignment:

Contig572___LKRCDANDSPLSPIGFMERAATVYGEMTSVIYNDSRFTWCQTFDRCXXXXXXXXXRNISRGDVVSVVAPNIPALYELHFAVPMAGAVLNNVNIRLNAPAMAIQFRHCQPKFVFVDYQYLPLVLEAI------KGTDHKPCLVVIEEESDTDRGKEYLDTTTKITTYHGLLREGDPVFEPRQPKDEWEPIVLNYTSGTTSAPKGVVNSHRAAYISTMNILSMWGIKPRPVFLWTLPMFHNNGWSFPWGIAATGGTNICLRKFDAKLIYSYIQDHGVTHFCAAPVVLSMIANASSSDRKPLPGRVEILTSGASPPSSDLLRMEELGFSVSHGYGLTESGGFVTLCAWKPEWDKLTAVDRSKLRVRQGVGVLTLVQVDVKDPASMVTVPRDGKTMGEIMLRGSTVMMGYLNDERSTAKAMEGGWFHTGDLGVIHSDGYLEIKDRSKDIIISGGENISSVEVESVLYCHPMIAEAAVVGRPDPFWGETPCAFVTNRVKASDPKLSGVRVSEAEMISFCRERLPHYMVPKTVVFMESLPKTSTGKIQKFLLREMAK
A9NWM6_____________LPKCPANYTALTPLWFIQRAALVYPKRKSVIHGARQYTWDQTYERCRRLASALAKRSIGPGTTVGVLAPNIPAAYEAHFGIPMAGAVLNSINIRLDARTIAFLLEHSRAVAVLVDQDFFMLLQEALGLMAKKHGSGFEPPFLVVIGDETCDEGNLKRALRSGAVEYEDFLQEGDPGFEWQPPDDEWQSIALGYTSGTTSSPKGVVLSHRGAYVASLGAALAWEMKGSGVYLWTLPMFHCNGWCYAWGMAAFCGTNICLRQVTARAIYASIAEHKVTHFCAAPVVLNTVVNCDPQDRVPIPRGISVMTAGSAPPASVLAKMEEQGFRVTHTYGLSETYGPSVVCAWKEEWDSLPPTDRARMNARQGVRYISLEGLDVVNPDSMVPVPADGSTVGEIVMRGNMMMKGYLRNPEANRESFRGGWFHSGDLAVKHPDGYIEIKDRSKDIIISGGENISSVEVENALYGHPRVLEASVVARPDERWGETPCAFITLK-GSGDPNEDESRIGQ-DIMNYCRARLPGYMVPRSVVF-GPLPKTATGKVQKHILRSKAK

SNPs (tot: 5)

Position	C %	G %	T %	Probability
858	10		90	0.90298
1319	14		85	0.993533
1667		90	10	0.90298
1670		90	10	0.90298
1672	10		90	0.90298

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain