SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene523

Length: 1286 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene523 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Heat shock protein 70 (Fragment) n=1 Tax=Picea glauca RepID=Q40846_PICGL	-	-	72%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	55%
Blast2go	heat shock	-	-	73%

Source	GOs	Term	Type	Identity
Blast2go	response to stress	GO:0006950	Biological Process	73%
Blast2go	ATP binding	GO:0005524	Molecular Function	73%
Blast2go	oxidoreductase activity	GO:0016491	Molecular Function	73%

Source	InterPros	Term	Type	Identity
Sma3	Heat shock protein 70, conserved site	IPR018181	-	-
Sma3	Heat shock protein Hsp70	IPR001023	-	-
Sma3	Heat shock protein 70	IPR013126	-	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NXU2

Fln msg: query STOP codon is far from subject stop. Distance to subject end: 19 aas, your sequence is shorter than subject: 392 - 651

Fln protein: DNRMVSHFVQEFQRKYKIDISSNARALRRLRSACERAKRELSSSVEAIVEVPCLQEGQDLHSKIRRAKFEQMNMDLFEKCIEIVAKCLEDTGMSKAQIDNVVLVGGSTRIPKVQELLKAFFDGQKELCKSIHPDEAVAYGASVQAAVLNGDNTANLILRDVTPLSLGVEIQGDMMSVLVPRNTQIPVKREDIYETVEDNQESATVCVYEGERPIVEHNNLLGEFDLGGLPSAPRGDVEIINSLEIDANGIVKVSAEHKTSGVKEAMIMTNDSSRLGDDEIAKMIAEAEKLKIEDQEWKETVELRNALDQYIYDMKKSLNNEKLSGEMKEKDVQNIKAGLEEVGEWLSENANAEKDDLVKKMKKVKRLCNKNRQEPIGAESKSSAIASKLHF*

Protein Length: 393

Fln nts: GACAACAGAATGGTGAGCCATTTTGTGCAGGAATTCCAAAGGAAATACAAAATCGACATATCTTCAAATGCAAGAGCGCTTAGGAGGCTTCGCAGTGCATGCGAGAGAGCCAAGAGAGAGCTGTCTTCCTCTGTGGAGGCCATCGTTGAAGTACCCTGTCTCCAAGAAGGCCAAGACCTTCATTCTAAAATAAGGCGAGCAAAGTTTGAGCAGATGAACATGGATCTGTTTGAAAAATGCATTGAAATTGTTGCCAAGTGCTTGGAGGATACTGGAATGAGTAAAGCCCAAATTGACAACGTTGTGTTGGTGGGAGGTTCTACGCGAATACCCAAAGTGCAGGAGTTGCTTAAAGCTTTCTTCGACGGCCAGAAGGAGTTGTGTAAGTCCATCCATCCTGATGAAGCTGTAGCTTATGGTGCTTCCGTGCAGGCTGCGGTGTTGAATGGCGACAACACAGCAAACCTCATACTTCGAGATGTCACTCCCCTTAGTCTAGGGGTTGAAATTCAAGGGGATATGATGAGCGTATTGGTCCCTAGAAATACACAAATTCCGGTTAAAAGGGAGGACATATATGAGACTGTAGAGGACAATCAGGAAAGTGCAACAGTATGTGTTTATGAAGGGGAAAGGCCTATTGTTGAACACAATAATCTTTTGGGGGAATTTGATTTGGGGGGACTTCCTTCTGCACCTAGAGGCGATGTCGAAATCATCAACAGTCTGGAGATCGATGCAAACGGAATTGTGAAGGTGTCAGCAGAGCATAAGACTAGTGGGGTAAAGGAAGCGATGATCATGACGAATGACAGCAGTAGGTTGGGCGATGATGAGATTGCCAAAATGATTGCAGAGGCAGAGAAACTCAAAATTGAAGATCAAGAATGGAAGGAAACAGTGGAACTGAGGAATGCACTGGATCAATACATCTACGACATGAAGAAAAGCTTGAATAATGAAAAACTGAGCGGAGAGATGAAGGAGAAAGATGTTCAAAACATCAAAGCAGGTCTTGAAGAGGTAGGTGAATGGCTCAGTGAAAATGCGAATGCAGAGAAGGATGACTTGGTAAAGAAAATGAAGAAAGTTAAAAGATTATGTAATAAAAATAGACAAGAGCCCATAGGGGCCGAGTCTAAATCATCTGCTATAGCATCCAAACTACATTTTTGA___GTTTGCTAAATCATTTCTCTATGTAAACCTGTTACATATCGATATTTACAATGCTTATATCATATTGTATATTGTAATTTGTCTCTGAATGATCAATGGTATGGATTGTG

Fln Alignment:

Contig523___DNRMVSHFVQEFQRKYKIDIXXXXXXXXXXXXACERAKRELSSSVEAIVEVPCLQEGQDLHSKIRRAKFEQMNMDLFEKCIEIVAKCLEDTGMSKAQIDNVVLVGGSTRIPKVQELLKAFFDGQKELCKSIHPDEAVAYGASVQAAVLNGDNTA---NLILRDVTPLSLGVEIQGDMMSVLVPRNTQIPVKREDIYETVEDNQESATVCVYEGERPIVEHNNLLGEFDLGGLPSAPRGDVEIINSLEIDANGIVKVSAEHKTSGVKEAMIMTNDSSRLGDDEIAKMIAEAEKLKIEDQEWKETVELRNALDQYIYDMKKSLNNEKLSGEMKEKDVQNIKAGLEEVGEWLSENANAE
A9NXU2_____________DNRMVNHFVQEFKRKHKKDISGNARALRRLRTACERAKRTLSSTTQTTIEIDSLYEGIDFYATITRARFEELNMDLFRRSMEPVEKCMRDAKMDKSQIDDVVLVGGSTRIPKVQQLLQDFFNG-KELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAGGVMTVLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELTGIPPAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNKITITNDKGRLSKEEIEKMVHDAEKYKAEDEEVKKKVDAKNSLENYAYNMRNTVRDEKFAGKLDPADKAKIEEAINGAISWLDQNQLAE

SNPs (tot: 8)

Position	A %	C %	G %	T %	Probability
917		66		33	1.0
1003	33	66			1.0
1008		71	28		1.0
1035		14	85		0.993533
1115		14		85	0.993533
1203	16	83			0.994512
1205		83		16	0.994512
1253	25		75		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain