SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene516

Length: 2428 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

>sp_v2.0_unigene516
CGGGTAAAATTTGCAGAGATACTCCTATCGAGTGCTTAAAGCCGCGACCGGGTCGTTCGCCGAGGGGCAAAAGCTGGGCCAGGGCGGGTTTGGGGCGGTTTACAAGGGGAAATTGCGTAACGGAACGGAAATTGCCGTCAAGGTGCTGGATACCAATTCCGTGCAGGGCGAGCGCGAGTTTCAGAATGAGGTTTCCGTTATAGGAAAGGTCAGCTCGCCGCACATAGTGGGCCTCCTGGGTTTCTGCGCGGATCGGAAAAGAAAGCTTCTGGTTTATGAATTTATGCGGAATCGGAGCCTTCAAGATGCTCTGTTTGGTGATAAATATTCTGTGCAGCTCGACTGGAAGAAAAGATTCGGGATTATGATGGATGCCGCCAAGGGCTTGGCTTTCTTGCATACCAAATGCGAGCCTGCAATAATTCATGGCGACATTAAGCCTAGCAATATTCTACTGGATGGCGGGTTCTCTGCTAGAATTGCCGATTTTGGCCTCGCCAGGCTGAAGGTAGAGAATCTGGAACAGCAGAAAGAGGATCTCGAGGGGCAGAAAGCCGAAGGAAAGAGGGATGAAGCGGCGCCGGTCGATGTGATCGCCGAAGATGATAAGTCGGTGAGGGAAGAAACGGAGAGCGTGATGACTGCGGAATTGGGATTCTCTGCTGTTGATGGAGATCCGGGGTGTCTGGGGAATATGCAGTCGCCGGAAACTTATGGTAGCAGTTCGGTATCGCCGGAGCAGAATCCGGGCTTTCTTTCGAGCAGTGCTTCTCCCGAGGGTTTGAATGATGAAACAGAAACGGTGGTGACCGAGACTCATGACGAAAGAACCCCCTCCGAGATTGTCGATTTGGAGAAAAACATGGGAATGTCGAGCAATGGGATCGTAGCCAAGACTGGATCTGTGGACTGCAGGAGCACCGATTTTTCAAGGGATGATGATGATAAAATGAGCATCGATAGTGGGAGAAAAAAGACTCCAACAAAGGATTGGTGGTGGAGGCAGCAGGACAGCAGTGGGGAATTCAGCGTTAAGGACTATGTTATGGAGTGGATTGGTACTGAAATTAAAAAGGAGAGGCCCAGCGGCAGCGAACGGGATTTGAACCCGGTTGGGAGGAGGAAGAAGCCAGAGAAGAGGCCGAGGAGGGAAAAAAGCTTCGAATGGTGGGTGACTCTTGCAGAGGAGGACGAGAGTGAAGAGCCCAAGAAGAAGGAGAAATCGAAGAACAGAGAGCGCAGGGAATGGTGGAGGGAAGAATTTTGCGAAGAATTGAGCCAGAAGAGCAACCGTAGCAGTAGACGAGTTAATAGCCGAAGCAGAAGCAGAGACTGGTTTCGCGATGAACCTCTTCCCCTCCACCACCATAGTAGGGATTGGGGTAGCGGAGATGTGATGAGAAGCGGAGTGAGCAGCACACCGAGCATGCGAGGCACCGTTTGCTACGTTGCTCCCGAGTATGGCGGTGGCGGTGCGCTCTCAGAGAGGGGCGATGTTTACAGTTTTGGGGTTCTTCTGTTGGTAATTATCTCCGGCCGGAGGCCTCTGCAGGTTATGGCGTCTCCGATGACACATTTCGAGAGAGCGAATCTCATATCGTGGGCCAGACACTTGGCTCGTTCGGGCAACGTCTTGGACCTCGTGGACGCCACGCTTGGTGAGGACTACGATAAAAGCCAGGCTCTGCTTTGCATCACCGTTGCTCTTCTCTGCCTGCAGAGATCGCCAGCAGCGAGACCCGCCATGACTGAGGTGGTCAAGATACTGTCAGGGGAAGTGGAGGCCCCGTCGCTTCCTTTCGAATTTTCTCCTTCGCCACCATATGGGTTTCCCTTTAGGTCGCGCAGGAAGGCCTCTTGCAGGGCTTCCCCTGCAATTCATGCATAAATGAGGCCTTCCCCTGCAATTCATGCATAAATTTTGTAGTGCCTCTTGCAGGGCTTCCCCTGCAATTCATGCATAAATTTTGTAGTGCTTATGTTTTTTTGGTGACTGCATCCATAAATTATTCCCATTCCCATTCCCATTCCAAATGTCACTTTTTTTGCTCTACAGGGCATGTACAATACATCTATTGGCAATACCCTGAGTCCGACTCCGAGCTGACTTTGTTCGAATGCCCAGATGGATTTTCAAAACTTTCATAGGAGTTACTGTAGAGACCGTGGGGTCTGGACTGCAAGCTTAGCTTTCATATTAATTTCACTTACAGATCTAATGTCCTTGAGCCGTAATAATGAATATATTCGTCCTTCTCATCGAAGCAATGTATATATATTTATTTTTAGCAGTATTGTCATAAAGGCATCGTACAGGGTAAGTTATTGCGAATTGCCAAGCATGGCTGTAGCCTTCAAACACGTTTTGAGAGTAGGTGAAACCCATCCTGGCAAGTAATCTCACATCAATTCTTCTGTCTGGCTTGCA

Ace file of the UniGene sp_v2.0_unigene516

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	protein kinase-like protein [Arabidopsis thaliana] sp\|O64639.1\|Y2559_ARATH RecName: Full=Receptor-like serine/threonine-protein kinase At2g45590 gb\|AAC06160.1\| putative protein kinase [Arabidopsis thaliana] gb\|AAO22616.1\| putative protein kinase [Arabidop	-	-	42%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	50%
Blast2go	receptor-like serine threonine-protein kinase at2g45590-like	-	-	66%

Source	ECs	Term	Type	e value	Identity
Blast2go	Transferases, Transferring phosphorous-containing groups, Protein-serine/threonine kinases.	EC:2.7.11.-	-	0.0	66%

Source	GOs	Term	Type	Identity
Blast2go	protein phosphorylation	GO:0006468	Biological Process	66%
Blast2go	ATP binding	GO:0005524	Molecular Function	66%
Blast2go	protein serine/threonine kinase activity	GO:0004674	Molecular Function	66%

Source	InterPros	Term	Type	Identity
Sma3	ATPase, F0 complex, subunit C	IPR000454	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Epidermal growth factor-like domain	IPR000742	-	-
Sma3	S-locus glycoprotein	IPR000858	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Bulb-type lectin domain	IPR001480	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	Alpha-isopropylmalate/homocitrate synthase, conserved site	IPR002034	-	-
Sma3	Lipocalin	IPR002345	-	-
Sma3	Gnk2-homologous domain	IPR002902	-	-
Sma3	Apple-like	IPR003609	-	-
Sma3	Sugar transporter, conserved site	IPR005829	-	-
Sma3	Phosphopantetheine attachment site	IPR006162	-	-
Sma3	Epidermal growth factor-like	IPR006210	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Leucine-rich repeat-containing N-terminal, type 2	IPR013210	-	-
Sma3	PAN-2 domain	IPR013227	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-
Sma3	SKG6/AXL2 alpha-helix transmembrane domain	IPR014805	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	Homeobox, conserved site	IPR017970	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: C0PRI9

Fln msg: Unexpected stop codon in the beginning of your sequence, your sequence is shorter than subject: 629 - 656

Fln protein: G*NLQRYSYRVLKAATGSFAEGQKLGQGGFGAVYKGKLRNGTEIAVKVLDTNSVQGEREFQNEVSVIGKVSSPHIVGLLGFCADRKRKLLVYEFMRNRSLQDALFGDKYSVQLDWKKRFGIMMDAAKGLAFLHTKCEPAIIHGDIKPSNILLDGGFSARIADFGLARLKVENLEQQKEDLEGQKAEGKRDEAAPVDVIAEDDKSVREETESVMTAELGFSAVDGDPGCLGNMQSPETYGSSSVSPEQNPGFLSSSASPEGLNDETETVVTETHDERTPSEIVDLEKNMGMSSNGIVAKTGSVDCRSTDFSRDDDDKMSIDSGRKKTPTKDWWWRQQDSSGEFSVKDYVMEWIGTEIKKERPSGSERDLNPVGRRKKPEKRPRREKSFEWWVTLAEEDESEEPKKKEKSKNRERREWWREEFCEELSQKSNRSSRRVNSRSRSRDWFRDEPLPLHHHSRDWGSGDVMRSGVSSTPSMRGTVCYVAPEYGGGGALSERGDVYSFGVLLLVIISGRRPLQVMASPMTHFERANLISWARHLARSGNVLDLVDATLGEDYDKSQALLCITVALLCLQRSPAARPAMTEVVKILSGEVEAPSLPFEFSPSPPYGFPFRSRRKASCRASPAIHA*

Protein Length: 630

Fln nts: CGGGTAAAATTTGCAGAGATACTCCTATCGAGTGCTTAAAGCCGCGACCGGGTCGTTCGCCGAGGGGCAAAAGCTGGGCCAGGGCGGGTTTGGGGCGGTTTACAAGGGGAAATTGCGTAACGGAACGGAAATTGCCGTCAAGGTGCTGGATACCAATTCCGTGCAGGGCGAGCGCGAGTTTCAGAATGAGGTTTCCGTTATAGGAAAGGTCAGCTCGCCGCACATAGTGGGCCTCCTGGGTTTCTGCGCGGATCGGAAAAGAAAGCTTCTGGTTTATGAATTTATGCGGAATCGGAGCCTTCAAGATGCTCTGTTTGGTGATAAATATTCTGTGCAGCTCGACTGGAAGAAAAGATTCGGGATTATGATGGATGCCGCCAAGGGCTTGGCTTTCTTGCATACCAAATGCGAGCCTGCAATAATTCATGGCGACATTAAGCCTAGCAATATTCTACTGGATGGCGGGTTCTCTGCTAGAATTGCCGATTTTGGCCTCGCCAGGCTGAAGGTAGAGAATCTGGAACAGCAGAAAGAGGATCTCGAGGGGCAGAAAGCCGAAGGAAAGAGGGATGAAGCGGCGCCGGTCGATGTGATCGCCGAAGATGATAAGTCGGTGAGGGAAGAAACGGAGAGCGTGATGACTGCGGAATTGGGATTCTCTGCTGTTGATGGAGATCCGGGGTGTCTGGGGAATATGCAGTCGCCGGAAACTTATGGTAGCAGTTCGGTATCGCCGGAGCAGAATCCGGGCTTTCTTTCGAGCAGTGCTTCTCCCGAGGGTTTGAATGATGAAACAGAAACGGTGGTGACCGAGACTCATGACGAAAGAACCCCCTCCGAGATTGTCGATTTGGAGAAAAACATGGGAATGTCGAGCAATGGGATCGTAGCCAAGACTGGATCTGTGGACTGCAGGAGCACCGATTTTTCAAGGGATGATGATGATAAAATGAGCATCGATAGTGGGAGAAAAAAGACTCCAACAAAGGATTGGTGGTGGAGGCAGCAGGACAGCAGTGGGGAATTCAGCGTTAAGGACTATGTTATGGAGTGGATTGGTACTGAAATTAAAAAGGAGAGGCCCAGCGGCAGCGAACGGGATTTGAACCCGGTTGGGAGGAGGAAGAAGCCAGAGAAGAGGCCGAGGAGGGAAAAAAGCTTCGAATGGTGGGTGACTCTTGCAGAGGAGGACGAGAGTGAAGAGCCCAAGAAGAAGGAGAAATCGAAGAACAGAGAGCGCAGGGAATGGTGGAGGGAAGAATTTTGCGAAGAATTGAGCCAGAAGAGCAACCGTAGCAGTAGACGAGTTAATAGCCGAAGCAGAAGCAGAGACTGGTTTCGCGATGAACCTCTTCCCCTCCACCACCATAGTAGGGATTGGGGTAGCGGAGATGTGATGAGAAGCGGAGTGAGCAGCACACCGAGCATGCGAGGCACCGTTTGCTACGTTGCTCCCGAGTATGGCGGTGGCGGTGCGCTCTCAGAGAGGGGCGATGTTTACAGTTTTGGGGTTCTTCTGTTGGTAATTATCTCCGGCCGGAGGCCTCTGCAGGTTATGGCGTCTCCGATGACACATTTCGAGAGAGCGAATCTCATATCGTGGGCCAGACACTTGGCTCGTTCGGGCAACGTCTTGGACCTCGTGGACGCCACGCTTGGTGAGGACTACGATAAAAGCCAGGCTCTGCTTTGCATCACCGTTGCTCTTCTCTGCCTGCAGAGATCGCCAGCAGCGAGACCCGCCATGACTGAGGTGGTCAAGATACTGTCAGGGGAAGTGGAGGCCCCGTCGCTTCCTTTCGAATTTTCTCCTTCGCCACCATATGGGTTTCCCTTTAGGTCGCGCAGGAAGGCCTCTTGCAGGGCTTCCCCTGCAATTCATGCATAA___ATGAGGCCTTCCCCTGCAATTCATGCATAAATTTTGTAGTGCCTCTTGCAGGGCTTCCCCTGCAATTCATGCATAAATTTTGTAGTGCTTATGTTTTTTTGGTGACTGCATCCATAAATTATTCCCATTCCCATTCCCATTCCAAATGTCACTTTTTTTGCTCTACAGGGCATGTACAATACATCTATTGGCAATACCCTGAGTCCGACTCCGAGCTGACTTTGTTCGAATGCCCAGATGGATTTTCAAAACTTTCATAGGAGTTACTGTAGAGACCGTGGGGTCTGGACTGCAAGCTTAGCTTTCATATTAATTTCACTTACAGATCTAATGTCCTTGAGCCGTAATAATGAATATATTCGTCCTTCTCATCGAAGCAATGTATATATATTTATTTTTAGCAGTATTGTCATAAAGGCATCGTACAGGGTAAGTTATTGCGAATTGCCAAGCATGGCTGTAGCCTTCAAACACGTTTTGAGAGTAGGTGAAACCCATCCTGGCAAGTAATCTCACATCAATTCTTCTGTCTGGCTTGCA

Fln Alignment:

Contig516___YSYRVLKAATGSFAEGQKLGQGGFGAVYKGKLRNGTEIAVKVLDTNSVQGEREFQNEVSVIGKVSSPHIVGLLGFCADRKRKLLVYEFMRNRSLQDALFGDKYSVQLDWKKRFGIMMDAAKGLAFLHTKCEPAIIHGDIKPSNILLDGGFSARIADFGLARLKVEN
C0PRI9_____________FTYEDLEAATNGFSRTNLLGQGGFGYVYKGILPGSKTIAVKQLKVGGSQGEREFQAEVEIISRVHHRHLVSLVGYCIAGSQRLLVYEFVPNDTLEHHLHG-KGQPNMEWPTRLKIAIGAARGLAYLHEDCYPKIIHRDIKASNILLDSNFEAKVADFGLAKLASED

SNPs (tot: 28)

Position	A %	C %	G %	T %	Probability
1374	25		75		0.996473
1376	75		25		0.996473
1523	16		50	33	1.0
1525			16	83	0.994512
1533	73		26		1.0
1703	13	86			1.0
1832		12	87		0.992554
1834			12	87	0.992554
1905		77		11	0.968132
2094		42	57		1.0
2138		14		85	0.993533
2139	14			85	0.993533
2141	85	14			0.993533
2152	90			10	0.90298
2155	90			10	0.90298
2158		10		90	0.90298
2162		10	90		0.90298
2163	10			90	0.90298
2173	10		90		0.90298
2191			10	90	0.90298
2193	90	10			0.90298
2197			11	88	0.923564
2198	11			88	0.923564
2200	88	11			0.923564
2204			11	88	0.923564
2205	88		11		0.923564
2206	88	11			0.923564
2208			11	88	0.923564

SSRs (tot: 2)

Start position	End position	Sequence	Length
935	946	GAT GAT GAT GAT	12
2009	2031	ATTCCC ATTCCC ATTCCC ATTCC	23

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain